ES2137468T3 - Oligonucleotido utilizable como iniciador en un procedimiento de amplificacion basado en una replica con desplazamiento de cadena. - Google Patents

Oligonucleotido utilizable como iniciador en un procedimiento de amplificacion basado en una replica con desplazamiento de cadena.

Info

Publication number
ES2137468T3
ES2137468T3 ES95402411T ES95402411T ES2137468T3 ES 2137468 T3 ES2137468 T3 ES 2137468T3 ES 95402411 T ES95402411 T ES 95402411T ES 95402411 T ES95402411 T ES 95402411T ES 2137468 T3 ES2137468 T3 ES 2137468T3
Authority
ES
Spain
Prior art keywords
replica
initiator
procedure based
amplification procedure
chain displacement
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES95402411T
Other languages
English (en)
Inventor
Philippe Cleuziat
Francoise Guillou-Bonnici
Pierre Levasseur
Francois Mallet
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biomerieux SA
Original Assignee
Biomerieux SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biomerieux SA filed Critical Biomerieux SA
Application granted granted Critical
Publication of ES2137468T3 publication Critical patent/ES2137468T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates

Abstract

LA INVENCION SE REFIERE A OLIGONUCLEOTIDOS COMPRENDIENDO, DESDE 5'' HACIA 3'', UNA PRIMERA PARTE QUE DISPONE DE UN SEGMENTO ENTRE ELLOS Y UN SEGMENTO QUE FORMA UNA ESTRUCTURA DE TRONCO DE BUCLE, CON UNA SEGUNDA PARTE DE AUTOAPAREAMIENTO NO SUSCEPTIBLE. LA PRIMERA PARTE COMPRENDE AL MENOS UN AGENTE DE BLOQUEO, CON CAPACIDAD DE BLOQUEAR LA REPLICA MEDIANTE UNA POLIMERASA DEL OLIGONUCLEOTIDO, DE FORMA QUE AL MENOS UNA PARTE DEL SEGMENTO SEA REPLICADA Y DE MANERA QUE EL SEGMENTO INTERMEDIO NO SEA REPLICADO NI TOTAL Y PARCIALMENTE. ESTE OLIGONUCLEOTIDO ES UTILIZADO COMO CEBO EN UN PROCESO DE AMPLIFICACION CICLICA DE UNA SECUENCIA CICLICA DE UN ACIDO NUCLEICO.
ES95402411T 1994-10-28 1995-10-27 Oligonucleotido utilizable como iniciador en un procedimiento de amplificacion basado en una replica con desplazamiento de cadena. Expired - Lifetime ES2137468T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR9413010A FR2726277B1 (fr) 1994-10-28 1994-10-28 Oligonucleotide utilisable comme amorce dans une methode d'amplification basee sur une replication avec deplacement de brin

Publications (1)

Publication Number Publication Date
ES2137468T3 true ES2137468T3 (es) 1999-12-16

Family

ID=9468373

Family Applications (1)

Application Number Title Priority Date Filing Date
ES95402411T Expired - Lifetime ES2137468T3 (es) 1994-10-28 1995-10-27 Oligonucleotido utilizable como iniciador en un procedimiento de amplificacion basado en una replica con desplazamiento de cadena.

Country Status (8)

Country Link
US (1) US5874260A (es)
EP (1) EP0713922B1 (es)
AT (1) ATE184324T1 (es)
CA (1) CA2161577C (es)
DE (1) DE69512003T2 (es)
DK (1) DK0713922T3 (es)
ES (1) ES2137468T3 (es)
FR (1) FR2726277B1 (es)

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Also Published As

Publication number Publication date
DE69512003T2 (de) 2000-05-25
CA2161577A1 (fr) 1996-04-29
DK0713922T3 (da) 2000-03-27
ATE184324T1 (de) 1999-09-15
DE69512003D1 (de) 1999-10-14
CA2161577C (fr) 2010-08-24
FR2726277A1 (fr) 1996-05-03
FR2726277B1 (fr) 1996-12-27
US5874260A (en) 1999-02-23
EP0713922B1 (fr) 1999-09-08
EP0713922A1 (fr) 1996-05-29

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