ES2128562T3 - Celulas deficientes en sialidasa. - Google Patents
Celulas deficientes en sialidasa.Info
- Publication number
- ES2128562T3 ES2128562T3 ES94916789T ES94916789T ES2128562T3 ES 2128562 T3 ES2128562 T3 ES 2128562T3 ES 94916789 T ES94916789 T ES 94916789T ES 94916789 T ES94916789 T ES 94916789T ES 2128562 T3 ES2128562 T3 ES 2128562T3
- Authority
- ES
- Spain
- Prior art keywords
- sialidase
- dna
- sialidasa
- deficient cells
- arn
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 230000002950 deficient Effects 0.000 title 1
- 102000005348 Neuraminidase Human genes 0.000 abstract 4
- 108010006232 Neuraminidase Proteins 0.000 abstract 4
- 241000699802 Cricetulus griseus Species 0.000 abstract 1
- 108020005187 Oligonucleotide Probes Proteins 0.000 abstract 1
- 150000001413 amino acids Chemical class 0.000 abstract 1
- 230000000692 anti-sense effect Effects 0.000 abstract 1
- 238000004113 cell culture Methods 0.000 abstract 1
- 239000012530 fluid Substances 0.000 abstract 1
- 239000002751 oligonucleotide probe Substances 0.000 abstract 1
- 210000001672 ovary Anatomy 0.000 abstract 1
- 230000006798 recombination Effects 0.000 abstract 1
- 238000005215 recombination Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/005—Glycopeptides, glycoproteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01018—Exo-alpha-sialidase (3.2.1.18), i.e. trans-sialidase
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- General Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Fats And Perfumes (AREA)
Abstract
UN LINEA CELULAR RECOMBINANTE QUE TIENE UNA SIALIDASA CONSTITUTIVA CUYA EXPRESION FUNCION QUEDA INTERRUMPIDA MEDIANTE, POR EJEMPLO, UNA RECOMBINACION HOMOLOGA O MEDIANTE LA UTILIZACION DE UN ARN ANTISENTIDO. LA SIALIDASA SE PURIFICA A PARTIR DEL FLUIDO DE UN CULTIVO DE CELULAS DE CELULAS DE LOS OVARIOS DE HAMSTERES CHINOS. EL ADN QUE CODIFICA LA SIALIDASA SE PUEDE OBTENER UTILIZANDO UNA SONDA DE OLIGONUCLEOTIDOS DISEÑADA UTILIZANDO LOS DATOS DE UNA SECUENCIA DE AMINOACIDOS SOBRE LA SIALIDASA Y EL ADN SE EXPRESA EN LAS CELULAS HUESPEDES TRANSFORMADAS CON EL ADN.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US6258693A | 1993-05-17 | 1993-05-17 | |
| US18732794A | 1994-01-25 | 1994-01-25 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2128562T3 true ES2128562T3 (es) | 1999-05-16 |
Family
ID=26742448
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES98106858T Expired - Lifetime ES2346941T3 (es) | 1993-05-17 | 1994-05-17 | Sialidasa recombinante de celula cho. |
| ES94916789T Expired - Lifetime ES2128562T3 (es) | 1993-05-17 | 1994-05-17 | Celulas deficientes en sialidasa. |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES98106858T Expired - Lifetime ES2346941T3 (es) | 1993-05-17 | 1994-05-17 | Sialidasa recombinante de celula cho. |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US5928915A (es) |
| EP (2) | EP0700443B1 (es) |
| JP (1) | JPH08510133A (es) |
| AT (2) | ATE474925T1 (es) |
| AU (2) | AU6834994A (es) |
| CA (1) | CA2160458A1 (es) |
| DE (2) | DE69415669T2 (es) |
| DK (2) | DK0866130T3 (es) |
| ES (2) | ES2346941T3 (es) |
| GR (1) | GR3029781T3 (es) |
| NZ (1) | NZ266714A (es) |
| PT (1) | PT866130E (es) |
| WO (1) | WO1994026908A1 (es) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6562588B2 (en) * | 1993-05-17 | 2003-05-13 | Genentech, Inc. | Sialidase and recombinant cell lines |
| JP3088681B2 (ja) * | 1997-05-22 | 2000-09-18 | 宮城県 | 形質膜局在シアリダーゼ及びそれをコードするｄｎａ |
| US6261805B1 (en) * | 1999-07-15 | 2001-07-17 | Boyce Thompson Institute For Plant Research, Inc. | Sialyiation of N-linked glycoproteins in the baculovirus expression vector system |
| KR100604258B1 (ko) * | 1999-12-30 | 2006-07-31 | 씨제이 주식회사 | 당단백질의 생체내 활성을 증가시키는 방법 |
| WO2003052104A1 (en) * | 2001-12-14 | 2003-06-26 | Provost, Fellows And Scholars Of The College Of The Holy And Undivided Trinity Of Queen Elizabeth Near Dublin | A reporter gene |
| US7781395B2 (en) * | 2004-01-30 | 2010-08-24 | Immunex Corporation | Process for purifying proteins |
| WO2007108464A1 (ja) * | 2006-03-20 | 2007-09-27 | Kyowa Hakko Kogyo Co., Ltd. | 哺乳動物由来細胞質シアリダーゼに対する抗体 |
| CN101636489B (zh) * | 2007-02-20 | 2014-08-13 | 帝斯曼知识产权资产管理有限公司 | 新颖的唾液酸酶 |
| US8076462B2 (en) | 2007-04-02 | 2011-12-13 | Kyowa Hakko Kirin Co., Ltd | Method for producing antithrombin composition |
| CN101896616B (zh) * | 2007-10-12 | 2014-06-04 | 西格马-奥利奇股份有限公司 | 提高糖蛋白唾液酸化作用的组合物与方法 |
| JP2008263996A (ja) * | 2008-06-02 | 2008-11-06 | National Institute Of Advanced Industrial & Technology | 発光甲虫由来赤色発光酵素安定体の生産及び精製法 |
| JP6482546B2 (ja) * | 2013-07-19 | 2019-03-13 | ラリクス・バイオサイエンス・リミテッド・ライアビリティ・カンパニーＬａｒｉｘ Ｂｉｏｓｃｉｅｎｃｅ， Ｌｌｃ | 二重対立遺伝子ノックアウトを生成するための方法および組成物 |
| US11060083B2 (en) | 2013-07-19 | 2021-07-13 | Larix Bioscience Llc | Methods and compositions for producing double allele knock outs |
| BR112016004437A2 (pt) * | 2013-09-13 | 2017-10-17 | Genentech Inc | métodos de imunoteste e de seleção de linhagem de células, anticorpos e kit |
| SG10201802525QA (en) | 2013-09-13 | 2018-04-27 | Genentech Inc | Methods and compositions comprising purified recombinant polypeptides |
| CN112795583A (zh) * | 2020-11-16 | 2021-05-14 | 上海大学 | 重组唾液酸外切酶的制备方法、表达基因、重组表达载体及构建方法 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4912040A (en) * | 1986-11-14 | 1990-03-27 | Genetics Institute, Inc. | Eucaryotic expression system |
| US5075218A (en) * | 1987-12-29 | 1991-12-24 | Biomira, Inc. | Screening for antibodies which bind carbohydrate epitopes of tumor-associated antigens, and uses thereof |
| US5047335A (en) * | 1988-12-21 | 1991-09-10 | The Regents Of The University Of Calif. | Process for controlling intracellular glycosylation of proteins |
| AU3144193A (en) * | 1991-11-21 | 1993-06-15 | Board Of Trustees Of The Leland Stanford Junior University | Controlling degradation of glycoprotein oligosaccharides by extracellular glycosisases |
-
1994
- 1994-05-17 AT AT98106858T patent/ATE474925T1/de active
- 1994-05-17 DE DE69415669T patent/DE69415669T2/de not_active Expired - Lifetime
- 1994-05-17 DK DK98106858.8T patent/DK0866130T3/da active
- 1994-05-17 EP EP94916789A patent/EP0700443B1/en not_active Expired - Lifetime
- 1994-05-17 JP JP6525776A patent/JPH08510133A/ja not_active Withdrawn
- 1994-05-17 AU AU68349/94A patent/AU6834994A/en not_active Abandoned
- 1994-05-17 ES ES98106858T patent/ES2346941T3/es not_active Expired - Lifetime
- 1994-05-17 NZ NZ266714A patent/NZ266714A/en not_active IP Right Cessation
- 1994-05-17 ES ES94916789T patent/ES2128562T3/es not_active Expired - Lifetime
- 1994-05-17 EP EP98106858A patent/EP0866130B1/en not_active Expired - Lifetime
- 1994-05-17 DE DE69435304T patent/DE69435304D1/de not_active Expired - Lifetime
- 1994-05-17 PT PT98106858T patent/PT866130E/pt unknown
- 1994-05-17 DK DK94916789T patent/DK0700443T3/da active
- 1994-05-17 WO PCT/US1994/005471 patent/WO1994026908A1/en active IP Right Grant
- 1994-05-17 AT AT94916789T patent/ATE175239T1/de active
- 1994-05-17 CA CA002160458A patent/CA2160458A1/en not_active Abandoned
-
1997
- 1997-06-09 US US08/871,074 patent/US5928915A/en not_active Expired - Lifetime
-
1998
- 1998-07-27 AU AU78419/98A patent/AU7841998A/en not_active Abandoned
-
1999
- 1999-03-23 GR GR990400869T patent/GR3029781T3/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| JPH08510133A (ja) | 1996-10-29 |
| NZ266714A (en) | 1997-12-19 |
| DK0866130T3 (da) | 2010-10-04 |
| CA2160458A1 (en) | 1994-11-24 |
| DE69415669D1 (de) | 1999-02-11 |
| DK0700443T3 (da) | 1999-08-30 |
| GR3029781T3 (en) | 1999-06-30 |
| EP0700443A1 (en) | 1996-03-13 |
| ES2346941T3 (es) | 2010-10-21 |
| EP0866130B1 (en) | 2010-07-21 |
| EP0700443B1 (en) | 1998-12-30 |
| WO1994026908A1 (en) | 1994-11-24 |
| PT866130E (pt) | 2010-10-26 |
| DE69415669T2 (de) | 1999-07-01 |
| US5928915A (en) | 1999-07-27 |
| DE69435304D1 (de) | 2010-09-02 |
| EP0866130A1 (en) | 1998-09-23 |
| ATE474925T1 (de) | 2010-08-15 |
| ATE175239T1 (de) | 1999-01-15 |
| AU7841998A (en) | 1998-10-01 |
| AU6834994A (en) | 1994-12-12 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG2A | Definitive protection |
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