DE69415669T2 - Sialidase defiziente zellen - Google Patents
Sialidase defiziente zellenInfo
- Publication number
- DE69415669T2 DE69415669T2 DE69415669T DE69415669T DE69415669T2 DE 69415669 T2 DE69415669 T2 DE 69415669T2 DE 69415669 T DE69415669 T DE 69415669T DE 69415669 T DE69415669 T DE 69415669T DE 69415669 T2 DE69415669 T2 DE 69415669T2
- Authority
- DE
- Germany
- Prior art keywords
- sialidase
- dna
- deficient cells
- disrupted
- purified
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 102000005348 Neuraminidase Human genes 0.000 title abstract 5
- 108010006232 Neuraminidase Proteins 0.000 title abstract 5
- 230000002950 deficient Effects 0.000 title 1
- 210000004027 cell Anatomy 0.000 abstract 2
- 108020005544 Antisense RNA Proteins 0.000 abstract 1
- 108020005187 Oligonucleotide Probes Proteins 0.000 abstract 1
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 1
- 239000012930 cell culture fluid Substances 0.000 abstract 1
- 210000004978 chinese hamster ovary cell Anatomy 0.000 abstract 1
- 239000003184 complementary RNA Substances 0.000 abstract 1
- 230000006801 homologous recombination Effects 0.000 abstract 1
- 238000002744 homologous recombination Methods 0.000 abstract 1
- 239000002751 oligonucleotide probe Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/005—Glycopeptides, glycoproteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01018—Exo-alpha-sialidase (3.2.1.18), i.e. trans-sialidase
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- General Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Fats And Perfumes (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US6258693A | 1993-05-17 | 1993-05-17 | |
| US18732794A | 1994-01-25 | 1994-01-25 | |
| PCT/US1994/005471 WO1994026908A1 (en) | 1993-05-17 | 1994-05-17 | Cho cell sialidase by recombinant dna technology |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE69415669D1 DE69415669D1 (de) | 1999-02-11 |
| DE69415669T2 true DE69415669T2 (de) | 1999-07-01 |
Family
ID=26742448
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69435304T Expired - Lifetime DE69435304D1 (de) | 1993-05-17 | 1994-05-17 | Rekombinante CHO Zell Sialidase |
| DE69415669T Expired - Lifetime DE69415669T2 (de) | 1993-05-17 | 1994-05-17 | Sialidase defiziente zellen |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69435304T Expired - Lifetime DE69435304D1 (de) | 1993-05-17 | 1994-05-17 | Rekombinante CHO Zell Sialidase |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US5928915A (de) |
| EP (2) | EP0700443B1 (de) |
| JP (1) | JPH08510133A (de) |
| AT (2) | ATE474925T1 (de) |
| AU (2) | AU6834994A (de) |
| CA (1) | CA2160458A1 (de) |
| DE (2) | DE69435304D1 (de) |
| DK (2) | DK0866130T3 (de) |
| ES (2) | ES2128562T3 (de) |
| GR (1) | GR3029781T3 (de) |
| NZ (1) | NZ266714A (de) |
| PT (1) | PT866130E (de) |
| WO (1) | WO1994026908A1 (de) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6562588B2 (en) * | 1993-05-17 | 2003-05-13 | Genentech, Inc. | Sialidase and recombinant cell lines |
| JP3088681B2 (ja) | 1997-05-22 | 2000-09-18 | 宮城県 | 形質膜局在シアリダーゼ及びそれをコードするｄｎａ |
| US6261805B1 (en) * | 1999-07-15 | 2001-07-17 | Boyce Thompson Institute For Plant Research, Inc. | Sialyiation of N-linked glycoproteins in the baculovirus expression vector system |
| KR100604258B1 (ko) * | 1999-12-30 | 2006-07-31 | 씨제이 주식회사 | 당단백질의 생체내 활성을 증가시키는 방법 |
| WO2003052104A1 (en) * | 2001-12-14 | 2003-06-26 | Provost, Fellows And Scholars Of The College Of The Holy And Undivided Trinity Of Queen Elizabeth Near Dublin | A reporter gene |
| WO2005075498A1 (en) * | 2004-01-30 | 2005-08-18 | Amgen Inc. | Process for purifying proteins |
| WO2007108464A1 (ja) * | 2006-03-20 | 2007-09-27 | Kyowa Hakko Kogyo Co., Ltd. | 哺乳動物由来細胞質シアリダーゼに対する抗体 |
| WO2008101893A2 (en) * | 2007-02-20 | 2008-08-28 | Dsm Ip Assets B.V. | Novel sialidase |
| WO2008120801A1 (ja) | 2007-04-02 | 2008-10-09 | Kyowa Hakko Kirin Co., Ltd. | アンチトロンビン組成物の製造方法 |
| JP2011500032A (ja) * | 2007-10-12 | 2011-01-06 | シグマ−アルドリッチ・カンパニー | 糖タンパク質のシアリル化を改善するための組成物および方法 |
| JP2008263996A (ja) * | 2008-06-02 | 2008-11-06 | National Institute Of Advanced Industrial & Technology | 発光甲虫由来赤色発光酵素安定体の生産及び精製法 |
| US11060083B2 (en) | 2013-07-19 | 2021-07-13 | Larix Bioscience Llc | Methods and compositions for producing double allele knock outs |
| US9663782B2 (en) | 2013-07-19 | 2017-05-30 | Larix Bioscience Llc | Methods and compositions for producing double allele knock outs |
| PL3044323T3 (pl) | 2013-09-13 | 2022-06-27 | F.Hoffmann-La Roche Ag | Sposoby wykrywania i ilościowego oznaczania białka komórki gospodarza w liniach komórkowych |
| NZ756750A (en) | 2013-09-13 | 2022-05-27 | Genentech Inc | Methods and compositions comprising purified recombinant polypeptides |
| CN112795583A (zh) * | 2020-11-16 | 2021-05-14 | 上海大学 | 重组唾液酸外切酶的制备方法、表达基因、重组表达载体及构建方法 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4912040A (en) * | 1986-11-14 | 1990-03-27 | Genetics Institute, Inc. | Eucaryotic expression system |
| US5075218A (en) * | 1987-12-29 | 1991-12-24 | Biomira, Inc. | Screening for antibodies which bind carbohydrate epitopes of tumor-associated antigens, and uses thereof |
| US5047335A (en) * | 1988-12-21 | 1991-09-10 | The Regents Of The University Of Calif. | Process for controlling intracellular glycosylation of proteins |
| WO1993010260A1 (en) * | 1991-11-21 | 1993-05-27 | The Board Of Trustees Of The Leland Stanford Junior University | Controlling degradation of glycoprotein oligosaccharides by extracellular glycosisases |
-
1994
- 1994-05-17 DE DE69435304T patent/DE69435304D1/de not_active Expired - Lifetime
- 1994-05-17 AT AT98106858T patent/ATE474925T1/de active
- 1994-05-17 AU AU68349/94A patent/AU6834994A/en not_active Abandoned
- 1994-05-17 PT PT98106858T patent/PT866130E/pt unknown
- 1994-05-17 AT AT94916789T patent/ATE175239T1/de active
- 1994-05-17 DK DK98106858.8T patent/DK0866130T3/da active
- 1994-05-17 DK DK94916789T patent/DK0700443T3/da active
- 1994-05-17 WO PCT/US1994/005471 patent/WO1994026908A1/en active IP Right Grant
- 1994-05-17 EP EP94916789A patent/EP0700443B1/de not_active Expired - Lifetime
- 1994-05-17 EP EP98106858A patent/EP0866130B1/de not_active Expired - Lifetime
- 1994-05-17 ES ES94916789T patent/ES2128562T3/es not_active Expired - Lifetime
- 1994-05-17 CA CA002160458A patent/CA2160458A1/en not_active Abandoned
- 1994-05-17 NZ NZ266714A patent/NZ266714A/en not_active IP Right Cessation
- 1994-05-17 ES ES98106858T patent/ES2346941T3/es not_active Expired - Lifetime
- 1994-05-17 DE DE69415669T patent/DE69415669T2/de not_active Expired - Lifetime
- 1994-05-17 JP JP6525776A patent/JPH08510133A/ja not_active Withdrawn
-
1997
- 1997-06-09 US US08/871,074 patent/US5928915A/en not_active Expired - Lifetime
-
1998
- 1998-07-27 AU AU78419/98A patent/AU7841998A/en not_active Abandoned
-
1999
- 1999-03-23 GR GR990400869T patent/GR3029781T3/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP0700443A1 (de) | 1996-03-13 |
| ATE474925T1 (de) | 2010-08-15 |
| DE69415669D1 (de) | 1999-02-11 |
| DK0700443T3 (da) | 1999-08-30 |
| PT866130E (pt) | 2010-10-26 |
| AU6834994A (en) | 1994-12-12 |
| GR3029781T3 (en) | 1999-06-30 |
| AU7841998A (en) | 1998-10-01 |
| EP0700443B1 (de) | 1998-12-30 |
| DK0866130T3 (da) | 2010-10-04 |
| CA2160458A1 (en) | 1994-11-24 |
| ATE175239T1 (de) | 1999-01-15 |
| US5928915A (en) | 1999-07-27 |
| ES2128562T3 (es) | 1999-05-16 |
| NZ266714A (en) | 1997-12-19 |
| ES2346941T3 (es) | 2010-10-21 |
| EP0866130B1 (de) | 2010-07-21 |
| JPH08510133A (ja) | 1996-10-29 |
| WO1994026908A1 (en) | 1994-11-24 |
| DE69435304D1 (de) | 2010-09-02 |
| EP0866130A1 (de) | 1998-09-23 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition |