ES2093174T3 - Procedimiento y equipo de ensayo para la deteccion de metabolismo microbiano. - Google Patents

Procedimiento y equipo de ensayo para la deteccion de metabolismo microbiano.

Info

Publication number
ES2093174T3
ES2093174T3 ES92200617T ES92200617T ES2093174T3 ES 2093174 T3 ES2093174 T3 ES 2093174T3 ES 92200617 T ES92200617 T ES 92200617T ES 92200617 T ES92200617 T ES 92200617T ES 2093174 T3 ES2093174 T3 ES 2093174T3
Authority
ES
Spain
Prior art keywords
dye
sample
procedure
detection
microbial metabolism
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES92200617T
Other languages
English (en)
Inventor
Thomas E Thompson
Ruth F Eden
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Difco Laboratories Inc
Original Assignee
Difco Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Difco Laboratories Inc filed Critical Difco Laboratories Inc
Application granted granted Critical
Publication of ES2093174T3 publication Critical patent/ES2093174T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2304/00Chemical means of detecting microorganisms
    • C12Q2304/20Redox indicators
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00Chemistry: analytical and immunological testing
    • Y10S436/80Fluorescent dyes, e.g. rhodamine

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Toxicology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

SE MUESTRA UN PROCESO Y EQUIPO PARA DETECTAR METABOLISMO MICROBIANO, INCLUYENDO EL PROCESO LOS PASOS DE COMBINAR MEDIO, UN TINTE METABOLICO CON UN ESPECTRO DE ABSORBENCIA QUE EN PRESENCIA DE MICROORGANISMOS CAMBIA Y RESPONDE A METABOLISMO DE LOS MICROORGANISMOS Y UN TINTE ANALITICO SEGUNDO CON UN ESPECTRO DE EXCITACION O EMISION QUE SE SUPERPONE CON EL ESPECTRO DE ABSORBENCIA CAMBIADO O NO CAMBIADO DEL TINTE PRIMERO. EL MEDIO CONTIENE UNA SOLUCION MUESTRA QUE SE ANALIZARA PARA CONTENER MICROORGANISMOS METABILIZANTES. EL USUARIO OBSERVA BIEN QUE NO SE HA PRODUCIDO NINGUN CAMBIO EN LAS EMISIONES DE FLUORESCENCIA DEL TINTE ANALITICO SEGUNDO LO QUE INDICA LA AUSENCIA DE MICROORGANISMOS METABILIZANTES EN LA MUESTRA O BIEN UN CAMBIO LO QUE INDICA LA PRESENCIA DE MICROORGANISMOS METABILIZANTES EN LA MUESTRA
ES92200617T 1990-06-22 1992-03-03 Procedimiento y equipo de ensayo para la deteccion de metabolismo microbiano. Expired - Lifetime ES2093174T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/542,115 US5164301A (en) 1990-06-22 1990-06-22 Process and kit for detecting microbial metabolism

Publications (1)

Publication Number Publication Date
ES2093174T3 true ES2093174T3 (es) 1996-12-16

Family

ID=24162403

Family Applications (1)

Application Number Title Priority Date Filing Date
ES92200617T Expired - Lifetime ES2093174T3 (es) 1990-06-22 1992-03-03 Procedimiento y equipo de ensayo para la deteccion de metabolismo microbiano.

Country Status (6)

Country Link
US (1) US5164301A (es)
EP (1) EP0558827B1 (es)
JP (1) JP3095833B2 (es)
AT (1) ATE144796T1 (es)
DE (1) DE69214931T2 (es)
ES (1) ES2093174T3 (es)

Families Citing this family (52)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5372784A (en) * 1988-08-31 1994-12-13 Baxter Diagnostics Inc. Measurement of bacterial CO2 production in an isolated fluorophore by monitoring an absorbance regulated change of fluorescence
US5716798A (en) * 1992-09-22 1998-02-10 Becton Dickinson And Company Enhanced detection of microorganisms in samples
US5580784A (en) * 1993-09-29 1996-12-03 Becton Dickinson And Company Data collection apparatus for use with chemical sensors
US5474910A (en) * 1993-10-15 1995-12-12 Alfano; Robert R. Method and device for detecting biological molecules and/or microorganisms within a desired area or space
US5620865A (en) * 1994-11-04 1997-04-15 Idexx Laboratories, Inc. Medium for detecting Enterococci in a sample
AU5094296A (en) * 1995-03-09 1996-10-02 Xechem, Inc. A rapid method of and diagnostic kit for the detection of microorganisms
US6046021A (en) * 1995-04-12 2000-04-04 Biolog, Inc. Comparative phenotype analysis of two or more microorganisms using a plurality of substrates within a multiwell testing device
US5627045A (en) * 1995-04-12 1997-05-06 Biolog, Inc. Multi-test format with gel-forming matrix for characterization of microorganisms
US6387651B1 (en) 1995-04-12 2002-05-14 Biolog, Inc. Comparative phenotype analysis of two or more microorganisms using a plurality of substrates within a microwell device
US5882882A (en) * 1995-04-12 1999-03-16 Biolog, Inc. Gel matrix with redox purple for testing and characterizing microorganisms
US5605812A (en) * 1995-05-19 1997-02-25 Charm Sciences, Inc. Test kit and method for the quantitative determination of coliform bacteria and E. coli
KR100436473B1 (ko) * 1995-06-05 2004-11-26 악조 노벨 엔.브이. 미생물검출장치및방법
DE19621312A1 (de) 1996-05-28 1997-12-04 Bayer Ag Maskierung der Hintergrundfluoreszenz und Signalverstärkung bei der optischen Analyse biologisch medizinischer Assays
WO1998004674A1 (en) 1996-07-26 1998-02-05 Idexx Laboratories, Inc. METHOD AND MEDIUM FOR DETECTING VANCOMYCIN-RESISTANT $i(ENTEROCOCCUS)
US5863751A (en) * 1996-09-30 1999-01-26 Becton Dickinson And Company Addition of lactose or other sugars in correcting false susceptibility results for resistant microorganisms in antimicrobial susceptibility tests
US6063591A (en) * 1997-05-14 2000-05-16 3M Innovative Properties Company System for measuring the efficacy of a sterilization cycle
US5863790A (en) * 1997-05-14 1999-01-26 Minnesota Mining And Manfacturing Company Biological sterility indicator
US6025189A (en) * 1997-05-14 2000-02-15 3M Innovative Properties Company Apparatus for reading a plurality of biological indicators
US6165741A (en) * 1997-05-30 2000-12-26 The Trustees Of The University Of Pennsylvania Method for rapid detection of bacterial growth in cultures
US6184040B1 (en) 1998-02-12 2001-02-06 Polaroid Corporation Diagnostic assay system and method
US6284526B1 (en) 1998-03-07 2001-09-04 Wardlaw Partners Lp Method and apparatus for determining the sensitivity of a microorganism to a growth altering agent
US6022734A (en) * 1998-03-07 2000-02-08 Wardlaw Partners, L.P. Disposable apparatus for determining antibiotic sensitivity of bacteria
US6140069A (en) * 1998-03-07 2000-10-31 Wardlaw Partners, Ll.P. Method for determining antibiotic sensitivity of bacteria
US6555060B1 (en) 1998-10-14 2003-04-29 Polaroid Corporation Apparatus for performing diagnostic testing
US6495373B1 (en) 1998-10-14 2002-12-17 Polaroid Corporation Method and apparatus for performing diagnostic tests
US6331715B1 (en) 1998-10-14 2001-12-18 Polaroid Corporation Diagnostic assay system and method having a luminescent readout signal
US6328930B1 (en) 1999-02-11 2001-12-11 Polaroid Corporation Apparatus for performing diagnostic testing
JP2002543434A (ja) * 1999-04-29 2002-12-17 デイド マイクロスキャン インコーポレーテッド 迅速な抗菌物質感受性アッセイと微生物同定を組み合わせたシステム
FI107167B (fi) 1999-06-07 2001-06-15 Marko Virta Menetelmä mikro-organismin kasvun ja kuolemisen määrittämiseksi
US6472166B1 (en) 2000-02-17 2002-10-29 Wardlaw Partners Lp Method for determining the effects of a growth-altering agent on a microbial colony
US6696239B1 (en) 2000-04-20 2004-02-24 Biolog, Inc. Comparative phenotype analysis for assessment of biological active compounds such as antimicrobials
DE10024086C1 (de) * 2000-05-18 2001-09-27 Merck Patent Gmbh Phenolfreie Färbelösung
WO2002055015A2 (en) * 2000-09-28 2002-07-18 Essential Therapeutics, Inc. Rapid antibiotic susceptibility test
US6641782B1 (en) 2000-11-15 2003-11-04 Polaroid Corporation Apparatus for performing diagnostic testing
FR2819266B1 (fr) * 2001-01-05 2004-01-16 Alain Rambach Milieu de culture pour la detection et/ou la discrimination des bacteries du genre listeria et procede de mise en oeuvre
CA2494182A1 (en) * 2002-08-07 2004-02-19 University Of South Florida Interpretation model for the uv-vis spectra of microorganisms
US20050112772A1 (en) * 2003-11-20 2005-05-26 Farone William A. Measurement of oxidation-reduction potential of a solution
CN1914332A (zh) * 2003-12-15 2007-02-14 快速实验室微系统公司 用于鉴定微生物的电化学分析
EP2647723B1 (en) 2004-06-23 2017-02-01 Zyzeba Testing Limited Improvements in and relating to micro-organism test apparatus and methods of using the same
WO2006022823A1 (en) * 2004-07-29 2006-03-02 Centrus International, Inc. Detection of microorganisms with a fluorescence-based device
US20080145316A1 (en) * 2006-12-14 2008-06-19 Macdonald John Gavin Skin coating with microbial indicator
US20080220465A1 (en) * 2007-03-05 2008-09-11 Pocared Diagnostics, Inc. Antibiotic Sensitivity Testing Method
WO2009091402A1 (en) * 2008-01-17 2009-07-23 Gideon Eden Co2 optical sensor for detection and enumeration of microorganisms
US8481302B2 (en) * 2008-11-03 2013-07-09 General Electric Company Total bacteria monitoring system
US20100112630A1 (en) * 2008-11-03 2010-05-06 Scott Martell Boyette Methods for measuring microbiological content in aqueous media
JP5816164B2 (ja) * 2009-05-07 2015-11-18 ビオメリュー・インコーポレイテッド 抗生物質耐性測定方法
CN102803504B (zh) 2009-06-15 2015-07-01 3M创新有限公司 产酸菌的检测
EP3551046B1 (en) 2016-12-07 2023-07-19 Biora Therapeutics, Inc. Gastrointestinal tract detection methods, devices and systems
JP7154615B2 (ja) * 2017-01-09 2022-10-18 ポカード・ディアグノスティクス・リミテッド 単一蛍光膜色素染色およびフローサイトメトリーによる特有のスペクトル強度比分析に基づく迅速抗菌薬感受性試験
WO2019108125A1 (en) * 2017-11-30 2019-06-06 Ascilion Ab A method for determining microbial susceptibility to antibiotic agents
FR3103900B1 (fr) * 2019-11-29 2024-07-19 Univ Du Mans Méthode d'identification rapide de microorganismes par analyse de matrices excitation-émission
CN116626001B (zh) * 2023-05-24 2024-04-05 中国矿业大学 一种基于荧光分光光度法测定硫酸盐还原菌在煤表面的吸附率的方法

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3564588A (en) * 1964-07-07 1971-02-16 Us Navy Chemiluminescent system for detecting living microorganisms
US3676679A (en) * 1970-04-22 1972-07-11 Johnston Lab Inc Apparatus for detecting biological activity
US3957583A (en) * 1974-12-02 1976-05-18 Mcdonnell Douglas Corporation Apparatus and process for determining the susceptibility of microorganisms to antibiotics
US4025393A (en) * 1975-11-17 1977-05-24 Block Engineering, Inc. System for detecting growth in microorganisms
US4116775A (en) * 1976-05-03 1978-09-26 Mcdonnell Douglas Corporation Machine and process for reading cards containing medical specimens
US4118280A (en) * 1976-05-03 1978-10-03 Mcdonnell Douglas Corporation Automated microbial analyzer
US4049499A (en) * 1976-07-01 1977-09-20 Corning Glass Works Microbial medium having fluorescent growth indicator
US4448534A (en) * 1978-03-30 1984-05-15 American Hospital Corporation Antibiotic susceptibility testing
US4311794A (en) * 1978-11-09 1982-01-19 Baylor College Of Medicine Determination of bacterial growth activity and antibiotic sensitivity by catalase measurement
US4221867A (en) * 1979-02-02 1980-09-09 Minnesota Mining And Manufacturing Company Optical microbiological testing apparatus and method
JPS5817598B2 (ja) * 1979-10-31 1983-04-08 味の素株式会社 微生物の迅速検出方法
US4250266A (en) * 1979-12-19 1981-02-10 Honeywell Inc. Automated micro-organism culture growth and detection instrument
CA1190838A (en) * 1981-07-17 1985-07-23 Cavit Akin Homogeneous nucleic acid hybridization diagnostics by non-radiative energy transfer
US4495293A (en) * 1983-02-24 1985-01-22 Abbott Laboratories Fluorometric assay
US4556636A (en) * 1983-06-09 1985-12-03 Eastman Kodak Company Composition, analytical element and method for the detection of bacteria
US4639421A (en) * 1984-10-01 1987-01-27 Becton, Dickinson And Company Fluorescent gram stain
US4665024A (en) * 1984-10-01 1987-05-12 Becton, Dickinson And Company Fluorescent gram stain
US4743561A (en) * 1985-03-05 1988-05-10 Abbott Laboratories Luminescent assay with a reagent to alter transmitive properties of assay solution
US4861709A (en) * 1985-05-31 1989-08-29 Technicon Research A.G. Detection and/or identification of microorganisms in a test sample using bioluminescence or other exogenous genetically-introduced marker
US4916060A (en) * 1985-09-17 1990-04-10 Massachusetts Institute Of Technology Process for chemical measurement in small volume samples by fluorescent indicators
US4859584A (en) * 1986-10-31 1989-08-22 Smithkline Beckman Corporation Cell growth rate determination by measurement of changes in cyanine dye levels in plasma membranes
FR2649410B2 (fr) * 1989-04-27 1994-08-19 Eurec Perfectionnement d'un milieu d'isolement pour l'identification de la bacterie salmonella

Also Published As

Publication number Publication date
ATE144796T1 (de) 1996-11-15
EP0558827B1 (en) 1996-10-30
EP0558827A1 (en) 1993-09-08
JP3095833B2 (ja) 2000-10-10
US5164301A (en) 1992-11-17
DE69214931D1 (de) 1996-12-05
JPH05137594A (ja) 1993-06-01
DE69214931T2 (de) 1997-06-05

Similar Documents

Publication Publication Date Title
ES2093174T3 (es) Procedimiento y equipo de ensayo para la deteccion de metabolismo microbiano.
Marose et al. Two‐dimensional fluorescence spectroscopy: A new tool for on‐line bioprocess monitoring
Wolfbeis et al. Reversible optical sensor membrane for hydrogen peroxide using an immobilized fluorescent probe, and its application to a glucose biosensor
ES2166429T3 (es) Dispositivo de analisis que comprende un colorante copulante estable, para la determinacion de analitos.
MY117657A (en) Diagnostics based on tetrazolium compounds
MY103023A (en) Composition and method for rapid detection of microorganisms in clinical samples.
EP1104772A4 (en) ANTIBODIES FOR DETECTING MICROORGANISMS
EP1221341B8 (de) Verfahren und Vorrichtung zur Bestimmung des Volumens einer Flüssigkeitsprobe
KR0171534B1 (en) Method for activation of polyanionic fluorescent dyes in flow dielectric media with quaternary onium compounds
ES2089009T3 (es) Aparato y metodos para ensayar la susceptibilidad antimicrobiana de microorganismos.
Sipior et al. Phase fluorometric optical carbon dioxide gas sensor for fermentation off‐gas monitoring
Taranova et al. Bacteria-degraders as the base of an amperometric biosensor for detection of anionic surfactants
ES2100141T1 (es) Ensayo biologico para contaminacion microbiana.
GB9002274D0 (en) Colorimetric analysis
Shelly et al. Multiparameter appraoch to the" fingerprinting" of fluorescent Pseudomonads.
IT1307270B1 (it) Procedimento e dispositivo per la determinazione della concentrazionedi eparina in un campione di fluido.
Lloyd Simultaneous dissolved oxygen and redox measurements: use of polarographic, bioluminescence and mass spectrometric monitoring combined with dual-wavelength spectrophotometry
Hume et al. Chemical tests for intoxication: what do the numbers really mean?
Podrazky et al. Determination of concentration of living immobilized yeast cells by fluorescence spectroscopy
JPH08173190A (ja) 微生物の存在の検査法および該検査法に使用する検査キット
Hartmann et al. Static and dynamic quenching of luminescent species in polymer media
Schaffar Biosensors based on fluorimetric fibre-optic detection
EP0231127A3 (en) Composition, element and method using polymeric mordants to increase the intensity of rigid fluorescent dyes
Heatley et al. The assay of micrococcin, an almost insoluble antibiotic
Schlager On-line fluorometric microbiological analysis for life support systems

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 558827

Country of ref document: ES