ES2078923T3 - Inmunoensayo enzimatico para detectar los antigenos y fase solida para su realizacion. - Google Patents

Inmunoensayo enzimatico para detectar los antigenos y fase solida para su realizacion.

Info

Publication number
ES2078923T3
ES2078923T3 ES90112454T ES90112454T ES2078923T3 ES 2078923 T3 ES2078923 T3 ES 2078923T3 ES 90112454 T ES90112454 T ES 90112454T ES 90112454 T ES90112454 T ES 90112454T ES 2078923 T3 ES2078923 T3 ES 2078923T3
Authority
ES
Spain
Prior art keywords
solid phase
derivative
substance
biotin
antibody
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90112454T
Other languages
English (en)
Inventor
Yoji Marui
Chozo Hayashi
Shigeki Ito
Mieko Fujio
Hiroki Tanaka
Toshihide Nagasaki
Yasuji Soda
Hitoshi Kaneta
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nippon Shoji Co Ltd
Original Assignee
Nippon Shoji Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=26411503&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2078923(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Nippon Shoji Co Ltd filed Critical Nippon Shoji Co Ltd
Application granted granted Critical
Publication of ES2078923T3 publication Critical patent/ES2078923T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57473—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving carcinoembryonic antigen, i.e. CEA
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57476—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving oncofetal proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6878—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids in eptitope analysis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Oncology (AREA)
  • Hospice & Palliative Care (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)

Abstract

UN INMUNOENSAYO DE ENZIMAS PARA UN ANTIGENO UTILIZANDO UNA FASE SOLIDA, QUE EMPLEA (A) UN ANTICUERPO BIOTINILATADO, EN EL QUE UN DERIVADO DE BIOTINA SE UNE A UN GRUPO TIOL DE UN FRAGMENTO FAB DE UN ANTICUERPO; (B) UN ANTICUERPO ETIQUETADO ENZIMA; Y (C) UNA FASE SOLIDA, INMOVILIZADA CON UNA SUSTANCIA CAPAZ DE REACCIONAR ESPECIFICAMENTE CON EL DERIVADO ANTERIOR DE BIOTINA, SELECCIONADO DE UN GRUPO FORMADO POR AVIDINA, ESTREPTOAVIDINA Y UN DERIVADO DE LAS MISMAS QUE SE UNE A LA FASE SOLIDA DIRECTAMENTE, O POR MEDIO DE UN ENLACE ENTRE OTRO DERIVADO DE BIOTINA Y UNA SUSTANCIA DE GRAN PESO MOLECULAR, UNIDA A LA FASE SOLIDA, O ALTERNATIVAMENTE EMPLEA (A'') UNA FASE SOLIDA INMOVILIZADA CON UN ANTICUERPO BIOTINILATADO, POR MEDIO DE UNA SUSTANCIA SELECCIONADA DE UN GRUPO FORMADO POR AVIDINA, ESTREPTOAVIDINA Y UN DERIVADO DE LAS MISMAS, DONDE LA CITADA SUSTANCIA SE UNE A LA MITAD DE LA BIOTINA DEL CITADO ANTICUERPO BIOTINILATADO Y TAMBIEN SE UNE A LA FASE SOLIDA DIRECTAMENTE, O MEDIANTE UN ENLACE ENTREOTRO DERIVADO DE BIOTINA Y UNA SUSTANCUIA DE GRAN PESO MOLECULAR, UNIDA A LA FASE SOLIDA, Y (B'') UN ANTICUERPO ETIQUETADO ENZIMA, Y UNA FASE SOLIDA UTILIZADA PARA EL MISMO.
ES90112454T 1989-06-29 1990-06-29 Inmunoensayo enzimatico para detectar los antigenos y fase solida para su realizacion. Expired - Lifetime ES2078923T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP16769789 1989-06-29
JP2070341A JP2619549B2 (ja) 1989-06-29 1990-03-20 抗原の定量法およびそれに用いる固相

Publications (1)

Publication Number Publication Date
ES2078923T3 true ES2078923T3 (es) 1996-01-01

Family

ID=26411503

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90112454T Expired - Lifetime ES2078923T3 (es) 1989-06-29 1990-06-29 Inmunoensayo enzimatico para detectar los antigenos y fase solida para su realizacion.

Country Status (8)

Country Link
US (1) US5378608A (es)
EP (2) EP0405578B1 (es)
JP (1) JP2619549B2 (es)
DE (1) DE69021953T2 (es)
DK (1) DK0405578T3 (es)
ES (1) ES2078923T3 (es)
FI (1) FI101022B (es)
NO (1) NO178047C (es)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6274325B1 (en) * 1990-06-25 2001-08-14 Boehringer Mannheim Gmbh Method for carrying out a homogeneous-immunoassay based on agglutination
JP2968039B2 (ja) * 1990-11-29 1999-10-25 株式会社京都第一科学 化学発光免疫測定法
DE4140142A1 (de) * 1991-12-05 1993-06-09 Boehringer Mannheim Gmbh, 6800 Mannheim, De Multivalentes dextranreagenz zum einsatz in praezipitationstests
ES2051247B1 (es) * 1992-11-27 1994-12-16 Univ Cordoba Procedimiento para la deteccion y medida de los niveles de hormona luteotropica porcina.
US5747352A (en) * 1994-05-23 1998-05-05 Beckman Instruments, Inc. Reagents and methods for the rapid and quantitative assay of pharmacological agents
DE19530078A1 (de) * 1995-08-16 1997-02-20 Bayer Ag Optischer Festphasenbiosensor auf Basis von Streptavidin und Biotin
DE19534988A1 (de) * 1995-09-21 1997-03-27 Forssmann Wolf Georg Verfahren zur Herstellung und Anwendung synthetischer, biotinylierter Peptide
US5958704A (en) * 1997-03-12 1999-09-28 Ddx, Inc. Sensing system for specific substance and molecule detection
US6303325B1 (en) 1998-05-29 2001-10-16 Dade Behring Inc. Method for detecting analytes
WO2000028326A1 (fr) * 1998-11-06 2000-05-18 Iatron Laboratories, Inc. Nouveaux complexes contenant de l'avidine reticulee, procede analytique dans lequel on utilise de l'avidine reticulee, reactifs et kits d'analyse
US7105311B2 (en) * 2001-05-03 2006-09-12 Immunetics, Inc. Systems and methods for detection of analytes in biological fluids
US20040209294A1 (en) * 2002-09-02 2004-10-21 National Food Research Institute Method to produce a receptor chip using biotinylated protein
AU2004210964A1 (en) * 2003-02-11 2004-08-26 University Of Washington Stimuli-responsive polymer conjugates and related methods
US20040248223A1 (en) * 2003-06-05 2004-12-09 Idexx Laboratories, Inc. Reduction of interfering peroxidase activity in peroxidase based detection methods
BRPI0718009B1 (pt) * 2006-11-01 2016-02-16 Beckman Coulter Inc suporte de ligação de afinidade, metodo para preparar o referido suporte, uso do mesmo, e ensaio de afinidade
US7981688B2 (en) 2007-03-08 2011-07-19 University Of Washington Stimuli-responsive magnetic nanoparticles and related methods
US8426214B2 (en) * 2009-06-12 2013-04-23 University Of Washington System and method for magnetically concentrating and detecting biomarkers
US9080933B2 (en) 2009-11-09 2015-07-14 University Of Washington Through Its Center For Commercialization Stimuli-responsive polymer diagnostic assay comprising magnetic nanoparticles and capture conjugates
US20110117668A1 (en) * 2009-11-09 2011-05-19 University Of Washington Through Its Center For Commercialization Self-powered smart diagnostic devices
EP2560003B1 (en) * 2010-04-14 2018-07-04 Eiken Kagaku Kabushiki Kaisha Complex of labeled probe and water-soluble carrier
WO2013016653A1 (en) 2011-07-28 2013-01-31 Cell Signaling Technology, Inc. Multi component detection

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE430062B (sv) * 1977-03-04 1983-10-17 Pharmacia Fine Chemicals Ab Kopplings- eller tioleringsreagens
JPS5510590A (en) * 1978-05-04 1980-01-25 Wellcome Found Enzyme immunity quantity analysis
US4282287A (en) * 1980-01-24 1981-08-04 Giese Roger W Biochemical avidin-biotin multiple-layer system
SE8003732L (sv) * 1980-05-19 1981-11-20 Pharmacia Diagnostics Ab Sett vid bestemningsmetoder involverande biospecifika affinitetsreaktioner
EP0072902B1 (de) * 1981-08-21 1985-04-24 F. HOFFMANN-LA ROCHE & CO. Aktiengesellschaft Verfahren zur Bestimmung von carcinoembryonalem Antigen (CEA) und zur Bestimmung geeignete Antikörperlösung
US4434150A (en) * 1981-10-19 1984-02-28 Ortho Diagnostic Systems, Inc. Immunological reagents employing polymeric backbone possessing reactive functional groups
US4429008B1 (en) * 1981-12-10 1995-05-16 Univ California Thiol reactive liposomes
GB8307156D0 (en) * 1983-03-15 1983-04-20 Celltech Ltd Heterogeneous binding assays
US4496654A (en) * 1983-04-08 1985-01-29 Quidel Detection of HCG with solid phase support having avidin coating
US4935339A (en) * 1985-05-07 1990-06-19 Nichols Institute Diagnostics Delayed solid phase immunologic assay
WO1987004794A1 (en) * 1986-02-06 1987-08-13 Microbiological Associates, Inc. Latex agglutination using avidin/biotin system
US4778751A (en) * 1986-05-12 1988-10-18 Diagnostic Products Corporation Method for measuring antigens or antibodies in biological fluids using ligand labeled antigens or ligand labeled antibodies
US4798795A (en) * 1987-02-17 1989-01-17 Hoechst Celanese Corporation Biotinylating agents
JPH07107537B2 (ja) * 1987-08-03 1995-11-15 イーストマン コダック カンパニー アビジン−及びビオチン−固定試薬，分析要素並びにその使用法
US4916075A (en) * 1987-08-19 1990-04-10 Ohmicron Corporation Differential homogeneous immunosensor device
JPS6488367A (en) * 1987-09-30 1989-04-03 Japan Chem Res Immobilized antibody
JPH03501777A (ja) * 1988-10-17 1991-04-18 モレキユラー　デヴアイシズ　コーポレイシヨン ハプテン誘導捕獲膜及び該膜を使用する診断アツセイ法

Also Published As

Publication number Publication date
FI101022B (fi) 1998-03-31
EP0405578A3 (en) 1991-05-29
JPH03128460A (ja) 1991-05-31
DE69021953D1 (de) 1995-10-05
DK0405578T3 (da) 1995-09-18
DE69021953T2 (de) 1996-04-04
NO178047C (no) 1996-01-10
FI903228A0 (fi) 1990-06-27
JP2619549B2 (ja) 1997-06-11
NO902899D0 (no) 1990-06-28
NO178047B (no) 1995-10-02
EP0405578A2 (en) 1991-01-02
EP0405578B1 (en) 1995-08-30
US5378608A (en) 1995-01-03
NO902899L (no) 1991-01-02
EP0620438A1 (en) 1994-10-19

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