ES2077058T3 - Procedimiento de recombinacion in vivo de secuencias de adn parcialmente homologas. - Google Patents

Procedimiento de recombinacion in vivo de secuencias de adn parcialmente homologas.

Info

Publication number
ES2077058T3
ES2077058T3 ES90900900T ES90900900T ES2077058T3 ES 2077058 T3 ES2077058 T3 ES 2077058T3 ES 90900900 T ES90900900 T ES 90900900T ES 90900900 T ES90900900 T ES 90900900T ES 2077058 T3 ES2077058 T3 ES 2077058T3
Authority
ES
Spain
Prior art keywords
dna sequences
vivo recombination
recombination procedure
recombination
partially approved
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90900900T
Other languages
English (en)
Inventor
Miroslav Cnrs Radman
Christiane Rayssiguier
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SETRATECH
Original Assignee
SETRATECH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by SETRATECH filed Critical SETRATECH
Application granted granted Critical
Publication of ES2077058T3 publication Critical patent/ES2077058T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

LA PRESENTE INVENCION SE REFIERE A UN PROCESO DE RECOMBINACION "IN VIVO" DE SECUENCIAS DE ADN PARCIALMENTE HOMOLOGAS QUE PRESENTAN HASTA 30 % DE DESEMPAREJAMIENTO DE BASES. SEGUN SU CARACTERISTICA ESENCIAL, DICHAS SECUENCIAS SE PONEN EN CONTACTO EN LAS CELULAS O EN SU ORGANISMO CUYO SISTEMA ENZIMATICO DE REPARACION DE LOS DESEMPAREJAMIENTOS ES DEFECTUOSO O HA ESTADO EN ACTIVO TRANSITORIAMENTE POR SATURACION DURANTE EL TIEMPO SUFICIENTE PARA OBTENER LA RECOMBINACION ENTRE DICHAS SECUENCIAS DE ADN O CUALQUIER OTRO MUTANTE QUE AUMENTE LA RECOMBINACION INTERGENETICA
ES90900900T 1988-12-26 1989-12-22 Procedimiento de recombinacion in vivo de secuencias de adn parcialmente homologas. Expired - Lifetime ES2077058T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR8817185A FR2641793B1 (fr) 1988-12-26 1988-12-26 Procede de recombinaison in vivo de sequences d'adn presentant des mesappariements de bases

Publications (1)

Publication Number Publication Date
ES2077058T3 true ES2077058T3 (es) 1995-11-16

Family

ID=9373422

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90900900T Expired - Lifetime ES2077058T3 (es) 1988-12-26 1989-12-22 Procedimiento de recombinacion in vivo de secuencias de adn parcialmente homologas.

Country Status (13)

Country Link
US (2) US5912119A (es)
EP (1) EP0449923B1 (es)
JP (1) JP3056524B2 (es)
AT (1) ATE127519T1 (es)
AU (1) AU4834390A (es)
CA (1) CA2006549C (es)
DE (1) DE68924174T2 (es)
ES (1) ES2077058T3 (es)
FR (1) FR2641793B1 (es)
IE (1) IE72469B1 (es)
IL (1) IL92856A (es)
WO (1) WO1990007576A1 (es)
ZA (1) ZA899902B (es)

Families Citing this family (64)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6165793A (en) 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US6406855B1 (en) 1994-02-17 2002-06-18 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6309883B1 (en) 1994-02-17 2001-10-30 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6395547B1 (en) 1994-02-17 2002-05-28 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US20060257890A1 (en) 1996-05-20 2006-11-16 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6995017B1 (en) 1994-02-17 2006-02-07 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
WO1997005268A1 (en) * 1995-07-26 1997-02-13 Setratech Homologous recombination in mismatch repair inactivated eukaryotic cells
US6489145B1 (en) 1996-07-09 2002-12-03 Diversa Corporation Method of DNA shuffling
US20020028443A1 (en) * 1999-09-27 2002-03-07 Jay M. Short Method of dna shuffling with polynucleotides produced by blocking or interrupting a synthesis or amplification process
CN1210557A (zh) * 1996-01-10 1999-03-10 诺沃挪第克公司 用于在细胞中体内产生突变体文库方法
US6165718A (en) 1996-01-10 2000-12-26 Novo Nordisk A/S Novo Alle Method for in vivo production of a mutant library in cells
US6506602B1 (en) 1996-03-25 2003-01-14 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US7115413B2 (en) 1996-04-01 2006-10-03 Mixis France S.A. Meiotic recombination in vivo of partially homologous DNA sequences
US6242211B1 (en) 1996-04-24 2001-06-05 Terragen Discovery, Inc. Methods for generating and screening novel metabolic pathways
US20070009930A1 (en) * 1996-12-18 2007-01-11 Maxygen, Inc. Methods and compositions for polypeptide engineering
EP0948615B1 (en) * 1996-12-20 2004-12-15 Novozymes A/S In vivo recombination
US7148054B2 (en) * 1997-01-17 2006-12-12 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
DK1717322T3 (da) 1997-01-17 2012-10-22 Codexis Mayflower Holdings Llc Udvikling af hele celler og organismer ved rekursiv sekvensrekombination
AU6029998A (en) 1997-01-17 1998-08-07 Regents Of The University Of Minnesota Dna molecules and protein displaying improved triazine compound degrading ability
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US6153410A (en) * 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
AUPO974597A0 (en) 1997-10-10 1997-11-06 Rhone-Poulenc Agro Methods for obtaining plant varieties
DK1036198T3 (da) 1997-12-08 2013-01-02 California Inst Of Techn Fremgangsmåde til fremstilling af polynukleotid- og polypeptidsekvenser
US6365408B1 (en) 1998-06-19 2002-04-02 Maxygen, Inc. Methods of evolving a polynucleotides by mutagenesis and recombination
US6846655B1 (en) * 1998-06-29 2005-01-25 Phylos, Inc. Methods for generating highly diverse libraries
US6438561B1 (en) * 1998-11-19 2002-08-20 Navigation Technologies Corp. Method and system for using real-time traffic broadcasts with navigation systems
US20030054390A1 (en) * 1999-01-19 2003-03-20 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US6368861B1 (en) 1999-01-19 2002-04-09 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US6917882B2 (en) * 1999-01-19 2005-07-12 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
WO2000042559A1 (en) * 1999-01-18 2000-07-20 Maxygen, Inc. Methods of populating data structures for use in evolutionary simulations
US6376246B1 (en) 1999-02-05 2002-04-23 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US7024312B1 (en) * 1999-01-19 2006-04-04 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
US20070065838A1 (en) * 1999-01-19 2007-03-22 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
EP1062614A1 (en) 1999-01-19 2000-12-27 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides
US6961664B2 (en) 1999-01-19 2005-11-01 Maxygen Methods of populating data structures for use in evolutionary simulations
US7026155B2 (en) 1999-02-02 2006-04-11 Regents Of The University Of California Method of reducing bacterial proliferation
US6518042B1 (en) * 1999-02-24 2003-02-11 Novozymes A/S Process for making DNA libraries in filamentous fungal cells using a novel cloned gene involved in the mismatch repair system of filamentous fungal cells
CN1341146A (zh) * 1999-02-24 2002-03-20 诺维信公司 具有灭活的dna错配修复系统的真菌细胞
US7430477B2 (en) * 1999-10-12 2008-09-30 Maxygen, Inc. Methods of populating data structures for use in evolutionary simulations
WO2002024890A2 (en) * 2000-09-18 2002-03-28 Pioneer Hi-Bred International, Inc. Rice mlh1 ortholog and uses thereof
EP1354064A2 (en) 2000-12-01 2003-10-22 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
CA2473741C (en) * 2002-01-18 2015-12-22 Morphotek, Inc. A method for generating engineered cells for locus specific gene regulation and analysis
SI1333095T1 (sl) 2002-01-31 2006-12-31 Mixis France Sa Postopek za reverzibilno funkcionalno inaktivacijo sistema za popravljanje neujemanja
EP1501927A2 (en) * 2002-05-02 2005-02-02 University Of North Carolina At Chapel Hill In vitro mutagenesis, phenotyping, and gene mapping
JP2008504832A (ja) * 2004-07-06 2008-02-21 ミクシス フランス ソシエテ アノニム バクテリオファージにおける組換え遺伝子の産生
EP1734125A1 (en) * 2005-06-16 2006-12-20 Institut National De La Recherche Agronomique Homeologous recombination in MSH2 inactivated plants or cells thereof
US20080124355A1 (en) * 2006-09-22 2008-05-29 David Gordon Bermudes Live bacterial vaccines for viral infection prophylaxis or treatment
US20100255487A1 (en) 2009-03-27 2010-10-07 Life Technologies Corporation Methods and apparatus for single molecule sequencing using energy transfer detection
SG184470A1 (en) 2010-04-09 2012-11-29 Eviagenics S A Method of generating gene mosaics
EP2677881B1 (en) 2011-02-24 2020-04-01 Erber Aktiengesellschaft Feed processing enzymes
AU2012274098A1 (en) 2011-06-21 2014-01-09 Eviagenics S.A. Method of metabolic evolution
CN104105793A (zh) 2011-10-12 2014-10-15 埃微杰尼克斯有限公司 在真核细胞中产生基因嵌合体的方法
EP2749644B1 (en) 2012-12-27 2018-08-22 Rhodia Operations Recombinant host cell for biosynthetic production of vanillin
CN105283547B (zh) 2012-12-27 2022-10-04 罗地亚经营管理公司 用于生物合成生产的重组宿主细胞
WO2014160418A2 (en) 2013-03-13 2014-10-02 GeneWeave Biosciences, Inc. Non-replicative transduction particles and transduction particle-based reporter systems
US9481903B2 (en) 2013-03-13 2016-11-01 Roche Molecular Systems, Inc. Systems and methods for detection of cells using engineered transduction particles
US9540675B2 (en) 2013-10-29 2017-01-10 GeneWeave Biosciences, Inc. Reagent cartridge and methods for detection of cells
US10351893B2 (en) 2015-10-05 2019-07-16 GeneWeave Biosciences, Inc. Reagent cartridge for detection of cells
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4863727A (en) * 1986-04-09 1989-09-05 Cetus Corporation Combination therapy using interleukin-2 and tumor necrosis factor
DE3724625A1 (de) * 1987-07-24 1989-02-02 Boehringer Mannheim Gmbh Enzymatisch inaktive, immunologisch aktive ss-galactosidase-muteine

Also Published As

Publication number Publication date
US5912119A (en) 1999-06-15
DE68924174D1 (de) 1995-10-12
ZA899902B (en) 1990-09-26
ATE127519T1 (de) 1995-09-15
EP0449923B1 (fr) 1995-09-06
WO1990007576A1 (fr) 1990-07-12
JP3056524B2 (ja) 2000-06-26
AU4834390A (en) 1990-08-01
IE72469B1 (en) 1997-04-09
US5965415A (en) 1999-10-12
FR2641793A1 (fr) 1990-07-20
IE894182L (en) 1990-06-26
EP0449923A1 (fr) 1991-10-09
DE68924174T2 (de) 1996-03-14
IL92856A0 (en) 1990-09-17
JPH04503601A (ja) 1992-07-02
CA2006549A1 (fr) 1990-06-26
CA2006549C (fr) 2000-09-05
FR2641793B1 (fr) 1993-10-01
IL92856A (en) 2001-04-30

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