ES2070823T3 - Liasa amonica de l-fenilalanina. - Google Patents

Liasa amonica de l-fenilalanina.

Info

Publication number
ES2070823T3
ES2070823T3 ES87308119T ES87308119T ES2070823T3 ES 2070823 T3 ES2070823 T3 ES 2070823T3 ES 87308119 T ES87308119 T ES 87308119T ES 87308119 T ES87308119 T ES 87308119T ES 2070823 T3 ES2070823 T3 ES 2070823T3
Authority
ES
Spain
Prior art keywords
pal
new
phenylalanine
production
liasa
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES87308119T
Other languages
English (en)
Inventor
Nobuhiro Fukuhara
Setsuo Yoshino
Kaoru Yamamoto
Tomoyuki Se
Satori Sone
Yoshiyuki Mitsui Toat Nakajima
Maki Mitsui Toatsu Iiji Suzuki
Nobuyoshi Makiguchi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mitsui Toatsu Chemicals Inc
Original Assignee
Mitsui Toatsu Chemicals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mitsui Toatsu Chemicals Inc filed Critical Mitsui Toatsu Chemicals Inc
Application granted granted Critical
Publication of ES2070823T3 publication Critical patent/ES2070823T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/22—Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
    • C12P13/222—Phenylalanine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

PARA PROPORCIONAR LA INFORMACION TECNICA NECESARIA PARA LA PRODUCCION DE UNA LIASA AMONIACAL DE L-FENILALANINA (PAL) MEDIANTE LA UTILIZACION DE TECNICAS DE INGENIERIA GENETICA, SE HAN DILUCIDADO EL GEN ESTRUCTURAL DE LA PAL Y LA SECUENCIA AMINOACIDA DE LA PAL EN RHODOSPORIDIUM TORULOIDES Y SE HAN CREADO NUEVOS PLASMIDOS DE ADN RECOMBINANTES (EJ., PSW101, PYTRP6 Y PKY201) MEDIANTE LA INSERCION DE UNA CEPA DE ADN QUE CODIFICA AL GEN DE LA PAL ENTRE EL TERMINO 3'' DE LA REGION PROMOTORA Y EL TERMINO 5'' DE LA REGION TERMINAL. ADEMAS, SE HAN CREADO TRANSFORMADORES QUE TIENEN TAL PLASMIDO NUEVO DE ADN RECOMBINANTE Y SE HA ESTABLECIDO UN PROCESO PARA LA PRODUCCION DE LA PAL MEDIANTE EL DESARROLLO DE TAL TRANSFORMADOR NUEVO PARA ASI CONSEGUIR QUE SE PRODUZCA LA PAL Y SE ACUMULE EN EL CULTIVO. ADEMAS, SE HA ESTABLECIDO UNA NUEVA TECNICA PARA LA PRODUCCION DE L-FENILALANINA MEDIANTE LA REACCION DE UN DONANTE DE AMONIACO CON ACIDO CINAMICO ANTE LA PRESENCIA DE LA PAL PREPARADA SEGUN EL NUEVO PROCESO ANTEDICHO.
ES87308119T 1986-09-16 1987-09-15 Liasa amonica de l-fenilalanina. Expired - Lifetime ES2070823T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP61215864A JP2507345B2 (ja) 1986-09-16 1986-09-16 Ｌ―フェニルアラニン―アンモニアリ・ア―ゼ構造遺伝子

Publications (1)

Publication Number Publication Date
ES2070823T3 true ES2070823T3 (es) 1995-06-16

Family

ID=16679534

Family Applications (1)

Application Number Title Priority Date Filing Date
ES87308119T Expired - Lifetime ES2070823T3 (es) 1986-09-16 1987-09-15 Liasa amonica de l-fenilalanina.

Country Status (8)

Country Link
US (1) US6472196B1 (es)
EP (1) EP0260919B1 (es)
JP (1) JP2507345B2 (es)
KR (1) KR910000461B1 (es)
CA (1) CA1341230C (es)
DE (1) DE3751106T2 (es)
DK (1) DK487787A (es)
ES (1) ES2070823T3 (es)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4946790A (en) * 1987-02-06 1990-08-07 Mitsui Toatsu Chemicals, Inc. Recombinant plasmid for the expression of L-phenylalanine ammonia-lyase and transformed strain carrying same
US5043277A (en) * 1987-02-19 1991-08-27 Mitsui Toatsu Chemicals, Incorporated Method of regulating expression of a foreign gene by controlling culture temperature and a process of producing a foreign gene product thereby
US5139935A (en) * 1987-02-19 1992-08-18 Mitsui Toatsu Chemicals, Incorporated Method of regulating expression of a foreign gene by controlling the sugar concentration in a medium and a process of producing a foreign product thereby
CA1319630C (en) * 1987-02-19 1993-06-29 Satori Sone Method of regulating expression of a foreign gene by controlling the sugar concentration in a medium and a process of producing a foreign gene product thereby
JP2507423B2 (ja) * 1987-05-22 1996-06-12 三井東圧化学株式会社 Ｌ―フェニルアラニン・アンモニアリア―ゼ
FR2632969B1 (fr) * 1988-06-16 1990-11-02 Ecole Nale Superieure Chimie Procede de synthese par voie enzymatique d'acides l-alpha-amines ou l-alpha-amino-n-hydroxyles
US6368837B1 (en) * 1999-08-06 2002-04-09 E. I. Du Pont Nemours And Company Bioproduction of para-hydroxycinnamic acid
JP4786844B2 (ja) * 1999-08-06 2011-10-05 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー パラ−ヒドロキシ桂皮酸の生物学的製造
US6521748B2 (en) 1999-08-06 2003-02-18 E. I. Du Pont De Nemours And Company Polynucleotide encoding a mutant Rhodotorula glutinis tyrosine ammonia lyase polypeptide

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1338400C (en) * 1983-08-31 1996-06-18 David H. Gelfand Recombinant fungal cellulases

Also Published As

Publication number Publication date
CA1341230C (en) 2001-05-15
KR880004088A (ko) 1988-06-01
DK487787D0 (da) 1987-09-16
JP2507345B2 (ja) 1996-06-12
DK487787A (da) 1988-05-17
DE3751106D1 (de) 1995-04-06
EP0260919B1 (en) 1995-03-01
KR910000461B1 (ko) 1991-01-25
DE3751106T2 (de) 1995-06-22
EP0260919A1 (en) 1988-03-23
JPS6371179A (ja) 1988-03-31
US6472196B1 (en) 2002-10-29

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