ES2052710T3 - Ensayo de ckmb, anticuerpos monoclonales para su uso en el mismo y lineas celulares hibridas. - Google Patents

Ensayo de ckmb, anticuerpos monoclonales para su uso en el mismo y lineas celulares hibridas.

Info

Publication number
ES2052710T3
ES2052710T3 ES88303056T ES88303056T ES2052710T3 ES 2052710 T3 ES2052710 T3 ES 2052710T3 ES 88303056 T ES88303056 T ES 88303056T ES 88303056 T ES88303056 T ES 88303056T ES 2052710 T3 ES2052710 T3 ES 2052710T3
Authority
ES
Spain
Prior art keywords
antibody
ckmb
creatine kinase
isoenzyme
hybrid cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES88303056T
Other languages
English (en)
Inventor
Uri Piran
Christine Anne Vitkauskas
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer Corp
Original Assignee
Ciba Corning Diagnosys Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=21886371&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2052710(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Ciba Corning Diagnosys Corp filed Critical Ciba Corning Diagnosys Corp
Application granted granted Critical
Publication of ES2052710T3 publication Critical patent/ES2052710T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

SE DESCRIBE UN METODO PARA DETECTAR EL ISOENZIMA MB DE CREATINA QUINASA EN UN FLUIDO BIOLOGICO, CARACTERIZADO PORQUE COMPRENDE: (A) PREPARAR UNA MEZCLA DE (I) UNA MUESTRA DEL FLUIDO BIOLOGICO, (II) UN ANTICUERPO DEL MONOMERO B DE CREATINA QUINASA, ESTANDO ENLAZADO DICHO ANTICUERPO A UN SOPORTE SOLIDO, Y (III) UN ANTICUERPO MONOCLONAL MARCADO AL ISOENZIMA MB DE LA CREATINA QUINASA; (B) INCUBAR LA MEZCLA; (C) SEPARAR EL SOPORTE SOLIDO DE LA MEZCLA; Y (D) DETECTAR LA CANTIDAD DE MARCA ASOCIADA CON EL SOPORTE SOLIDO. TAMBIEN SE DESCRIBE UN ANTICUERPO MONOCLONAL CARACTERIZADO PORQUE SE PRODUCE POR UNA FAMILIA DE CELULAS HIBRIDAS DERIVADAS DE LA MURINA, SIENDO CAPAZ DICHO ANTICUERPO DE UNIRSE ESPECIFICAMENTE A POR LO MENOS UN DETERMINADO ANTIGENO DEL ISOENZIMA MB DE LA CREATINA QUINASA HUMANA, Y QUE ES DEL TIPO IGG1. SE DESCRIBE A DEMAS UNA FAMILIA DE CELULAS HIBRIDAS DERIVADAS DELA MURINA ATCC HB 9389 O HB 9388 Y SUS MUTANTES. MAS PARTICULARMENTE, SE PROPORCIONA UN ENSAYO DE INCUBACION CKMB SENCILLA QUE EMPLEA UN ANTICUERPO DE CAPTURA ANTI CKB UNIDO A PARTICULAS MAGNETICAS Y UN ANTICUERPO TRAZADOR CKMB MARCADO CON UN ESTER DE ACRIDINIO. EL ENSAYO LOGRA UNA DOSIS MINIMA DETECTABLE DE POR LO MENOS 1 MG/L DE CKMB EN INCUBACIONES TAN CORTAS COMO 10 MINUTOS A TEMPERATURA AMBIENTE Y NO ESTA SOMETIDO A INTERFERENCIA DEBIDA A LA PRESENCIA DE MACRO-CK-1 O DE ALTOS NIVELES DE CKMM O CKBB DE MUESTRAS DE SUERO. DE ACUERDO CON CIERTAS INCORPORACIONES PREFERIDAS DE LA INVENCION, EL ANTICUERPO TRAZADOR ANTI-CKMB ES UN ANTICUERPO MONOCLONAL DE RATON DEL TIPO IGG1. EL PRESENTE ENSAYO ES MAS RAPIDO, MAS SENCILLO Y MENOS SOMETIDO A INTERFERENCIA QUE ENSAYOS DE TECNICAS ANTERIORES Y TIENE SU SENSIBILIDAD.
ES88303056T 1987-04-09 1988-04-06 Ensayo de ckmb, anticuerpos monoclonales para su uso en el mismo y lineas celulares hibridas. Expired - Lifetime ES2052710T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US3605787A 1987-04-09 1987-04-09

Publications (1)

Publication Number Publication Date
ES2052710T3 true ES2052710T3 (es) 1994-07-16

Family

ID=21886371

Family Applications (1)

Application Number Title Priority Date Filing Date
ES88303056T Expired - Lifetime ES2052710T3 (es) 1987-04-09 1988-04-06 Ensayo de ckmb, anticuerpos monoclonales para su uso en el mismo y lineas celulares hibridas.

Country Status (7)

Country Link
EP (1) EP0288179B1 (es)
JP (1) JP2657388B2 (es)
AT (1) ATE78102T1 (es)
AU (1) AU598358B2 (es)
CA (1) CA1340557C (es)
DE (1) DE3872575T2 (es)
ES (1) ES2052710T3 (es)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IE59210B1 (en) * 1985-11-14 1994-01-26 Univ Washington Creatine kinase mb determination method
CA2027434C (en) * 1990-10-12 1999-01-05 George Jackowski Diagnostic kit for diagnosing and distinguishing chest pain in early onset thereof
DE69228817T2 (de) * 1991-07-26 1999-09-23 Dade Chemistry Systems Inc., Deerfield Signalerkennungspruefung in der anwesenheit von einem suspendierten festen träger
DE4314254A1 (de) * 1993-04-30 1994-11-03 Boehringer Mannheim Gmbh Monoklonale Antikörper gegen CK-MB
US5650334A (en) * 1995-08-31 1997-07-22 First Medical, Inc. Fluorescent labelling compositions and methods for their use
US6299839B1 (en) 1995-08-31 2001-10-09 First Medical, Inc. System and methods for performing rotor assays
CN102435738A (zh) * 2011-08-31 2012-05-02 内蒙古科慧生物科技有限责任公司 肌酸激酶同工酶(ck-mb)定量测定试剂盒及其检测方法
CN103278623B (zh) * 2013-06-14 2015-09-23 博奥赛斯（天津）生物科技有限公司 一种肌酸激酶同功酶纳米磁微粒化学发光免疫定量检测试剂盒及其制备方法
CN105132384B (zh) * 2015-07-25 2018-07-03 大庆麦伯康生物技术有限公司 抗ck-mb单克隆抗体产生的杂交瘤
CN105435867B (zh) * 2015-10-26 2018-05-22 深圳华迈兴微医疗科技有限公司 检测全血中肌酸激酶同工酶的磁微粒化学发光微流控芯片
CN111349168B (zh) * 2018-12-20 2022-06-03 东莞市朋志生物科技有限公司 一种抗人ckmb的抗体及其应用
CN112979816B (zh) * 2019-12-18 2022-11-08 菲鹏生物股份有限公司 针对ckmb的结合蛋白及其应用
CN113004411B (zh) * 2019-12-18 2022-11-08 菲鹏生物股份有限公司 可特异性结合ckmb的结合蛋白、其应用以及检测ckmb的方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IE59210B1 (en) * 1985-11-14 1994-01-26 Univ Washington Creatine kinase mb determination method

Also Published As

Publication number Publication date
AU1409688A (en) 1988-10-13
JP2657388B2 (ja) 1997-09-24
CA1340557C (en) 1999-05-25
ATE78102T1 (de) 1992-07-15
JPH01262471A (ja) 1989-10-19
AU598358B2 (en) 1990-06-21
DE3872575D1 (de) 1992-08-13
EP0288179B1 (en) 1992-07-08
DE3872575T2 (de) 1992-12-24
EP0288179A1 (en) 1988-10-26

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