ES2047749T3 - Procedimiento para la deteccion de sustancias capaces de fijarse especificamente en liquidos corporales. - Google Patents

Procedimiento para la deteccion de sustancias capaces de fijarse especificamente en liquidos corporales.

Info

Publication number
ES2047749T3
ES2047749T3 ES90108656T ES90108656T ES2047749T3 ES 2047749 T3 ES2047749 T3 ES 2047749T3 ES 90108656 T ES90108656 T ES 90108656T ES 90108656 T ES90108656 T ES 90108656T ES 2047749 T3 ES2047749 T3 ES 2047749T3
Authority
ES
Spain
Prior art keywords
ligand
coating
procedure
detection
carbohydrate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90108656T
Other languages
English (en)
Inventor
Beatus Dr Ofenloch-Hahnle
Michael Dr Berger
Arno Dr Deger
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Application granted granted Critical
Publication of ES2047749T3 publication Critical patent/ES2047749T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/537—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody
    • G01N33/538—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody by sorbent column, particles or resin strip, i.e. sorbent materials
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/962—Prevention or removal of interfering materials or reactants or other treatment to enhance results, e.g. determining or preventing nonspecific binding
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25125—Digestion or removing interfering materials

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PARA COMPROBAR LA EXISTENCIA DE SUSTANCIAS ESPECIFICAS LIGANTES EN LIQUIDOS CORPORALES SEGUN EL PRINCIPIO DEL ENSAYO DE INMUNIDAD UTILIZANDO CONJUGADOS LIGANTES PARA LA MARCACION, INMOVILIZACION O COMPLEXACION QUE CONTIENEN EN FORMA LIBRE LOS LIGADOS QUE SE HAN UTILIZADO PARA EL CONJUGADO LIGANTE, SE EXTRAEN LOS LIGANTES PRESENTES EN FORMA LIBRE DEL LA REACCION INMUNOLOGICA INCUBANDO LA SOLUCION DE ENSAYO CON PARTICULAS DE POLIMEROS, QUE CONSISTEN DE UN NUCLEO Y ENVOLTURA, QUE COMO NUCLEO TIENEN UN POLIMERO QUE DISPONE DE UNA CANTIDAD DE PUNTOS DE LIGADURA PARA EL LIGANTE Y COMO ENVOLTURA POR LO MENOS UNA CAPA DE PROTEINA, PEPTICOS, POLIPOLIMEROS DE ACIDO NUCLEINICO, HIDRATOS DE CARBONO Y/O UN COPOLIMERISATO DE HIDRATOS DE CARBONO Y AMINOACIDOS. EL ESPESOR DE LA CAPA DE LA ENVOLTURA SE AJUSTA DE FORMA QUE EL REVESTIMIENTO SEA PERMEABLE PARA EL LIGANTE LIBRE PERO NO PARA EL CONJUGADO LIGANTE.
ES90108656T 1989-05-09 1990-05-08 Procedimiento para la deteccion de sustancias capaces de fijarse especificamente en liquidos corporales. Expired - Lifetime ES2047749T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE3915135A DE3915135A1 (de) 1989-05-09 1989-05-09 Verfahren zum nachweis spezifisch bindefaehiger substanzen in koerperfluessigkeiten

Publications (1)

Publication Number Publication Date
ES2047749T3 true ES2047749T3 (es) 1994-03-01

Family

ID=6380321

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90108656T Expired - Lifetime ES2047749T3 (es) 1989-05-09 1990-05-08 Procedimiento para la deteccion de sustancias capaces de fijarse especificamente en liquidos corporales.

Country Status (7)

Country Link
US (1) US5212063A (es)
EP (1) EP0397113B1 (es)
JP (1) JPH07117536B2 (es)
AT (1) ATE97496T1 (es)
DE (2) DE3915135A1 (es)
DK (1) DK0397113T3 (es)
ES (1) ES2047749T3 (es)

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WO1995023801A1 (de) * 1994-03-05 1995-09-08 Boehringer Mannheim Gmbh Entstörmittel zum einsatz bei immunoassays
AU6720096A (en) 1995-08-09 1997-03-05 Quidel Corporation Test strip and method for one step lateral flow assay
ES2127691B1 (es) * 1996-10-21 2000-04-01 Biolatex S L Procedimiento de union covalente de ligandos para la preparacion de r eactivos inmunoquimicos para utilizacion en inmunoanalisis homogeneos que emplean microparticulas (mps) y su aplicacion a la medida de ferritina y del receptor soluble de la transferrina en suero o plasma sangu
JPH10246725A (ja) * 1997-03-03 1998-09-14 Mikio Nakayama 抗体又は抗原の検出方法
US5958704A (en) * 1997-03-12 1999-09-28 Ddx, Inc. Sensing system for specific substance and molecule detection
DE19724787A1 (de) * 1997-06-06 1998-12-10 Biotez Berlin Buch Gmbh Bioche Streptavidin/Avidin beschichtete Oberflächen
US6410692B2 (en) * 1998-02-02 2002-06-25 Novadx, Inc. Removal of abundant interfering proteins from a liquid sample using a collapsible affinity matrix
US6303325B1 (en) * 1998-05-29 2001-10-16 Dade Behring Inc. Method for detecting analytes
US6143507A (en) * 1998-10-29 2000-11-07 Boehringer Ingelheim Pharmaceuticals, Inc. High throughput compatible assay for receptor-TRAF interactions
EP1215496A4 (en) * 1999-09-13 2005-06-08 Nissui Pharm Co Ltd METHOD AND KIT FOR DETECTING OR DETERMINING A SUBSTANCE
DE10009503A1 (de) * 2000-02-29 2001-08-30 Roche Diagnostics Gmbh Verfahren zur Immobilisierung von Konjugaten in diagnostischen Tests
EP1184666A3 (en) * 2000-08-21 2002-04-10 Roche Diagnostics GmbH Auxiliary haptens and haptenylated agents in displacement immunoassays
WO2002044695A1 (en) * 2000-11-16 2002-06-06 Burstein Technologies, Inc. Methods and apparatus for detecting and quantifying lymphocytes with optical biodiscs
CA2446246A1 (en) * 2001-05-03 2002-11-14 Immunetics, Inc. Systems and methods for detection of analytes in biological fluids
JP4839530B2 (ja) * 2001-06-08 2011-12-21 東ソー株式会社 測定妨害を低減する方法及び試薬組成物
US20030143637A1 (en) * 2001-08-31 2003-07-31 Selvan Gowri Pyapali Capture layer assemblies for cellular assays including related optical analysis discs and methods
AU2002335715A1 (en) * 2001-09-07 2003-03-24 Burstein Technologies, Inc. Optical bio-disc systems for nuclear morphology based identification
EP1448992A4 (en) * 2001-11-20 2005-11-02 Burstein Technologies Inc OPTICAL BIODISTICS AND FLUIDIC CIRCUITS FOR CELL ANALYSIS AND CORRESPONDING METHODS
US7108993B2 (en) * 2002-07-19 2006-09-19 Bayer Healthcare Llc Use of dual conjugated labels in the elimination of serum interference in immunochromatographic assays
US20050112586A1 (en) 2003-11-24 2005-05-26 Roland Janzen Method and composition for stabilizing liquid reagents
US20060046310A1 (en) * 2004-08-25 2006-03-02 Zong-Li Xia Amplification method for solid phase immunoassays
EP2541250A1 (en) * 2011-06-30 2013-01-02 Koninklijke Philips Electronics N.V. Molecular architecture on magnetic particles for affinity assays with low non-specific binding
EP3338094B1 (en) 2015-08-20 2019-05-08 H. Hoffnabb-La Roche Ag Particle-based immunoassay using a pegylated analyte-specific binding agent
JP7068323B2 (ja) 2017-02-02 2022-05-16 エフ．ホフマン－ラ ロシュ アーゲー 少なくとも２種のペグ化された分析物特異的結合剤を使用する免疫アッセイ
EP3791167A1 (en) 2018-05-07 2021-03-17 Immundiagnostik AG System for analysing quantitative lateral flow chromatography
CN112703400B (zh) * 2018-09-25 2024-10-15 美国西门子医学诊断股份有限公司 用于从使用环糊精阱的测定除去生物素干扰的方法和组合物
EP3857226B1 (en) * 2018-09-25 2023-11-15 Siemens Healthcare Diagnostics Inc. Methods and compositions for removing biotin interference from assays using conjugated molecular traps
WO2020068541A1 (en) 2018-09-25 2020-04-02 Siemens Healthcare Diagnostics Inc. Methods and compositions for removing biotin interference from assays using molecular traps
WO2020068548A1 (en) 2018-09-25 2020-04-02 Siemens Healthcare Diagnostics Inc. Compositions, kits, and methods for multiplex assays to correct for biotin interference in target analyte measurements
CN111122842A (zh) * 2018-10-31 2020-05-08 博阳生物科技（上海）有限公司 一种抗干扰剂及其应用
CN111122845A (zh) * 2018-10-31 2020-05-08 博阳生物科技（上海）有限公司 一种抗生物素干扰的化学发光免疫分析试剂盒及其应用
CN111122851A (zh) * 2018-10-31 2020-05-08 博阳生物科技（上海）有限公司 一种抗干扰剂的制备方法及由此方法制备的抗干扰剂的应用
WO2020165456A1 (en) 2019-02-15 2020-08-20 Immundiagnostik Ag Rapid test for diagnosis of bacterial infections in neonates
CN112129933B (zh) * 2019-06-25 2024-01-05 迈克生物股份有限公司 一种免疫分析系统中抗生物素干扰的试剂、试剂盒及方法
WO2023114993A1 (en) * 2021-12-17 2023-06-22 Siemens Healthcare Diagnostics Inc. Biotin-trap beads and methods of production and use thereof

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US3951748A (en) * 1974-11-11 1976-04-20 Medical Products, Inc. Sensitized matrix for detection of disease
US4282287A (en) * 1980-01-24 1981-08-04 Giese Roger W Biochemical avidin-biotin multiple-layer system
US4550075A (en) * 1983-06-22 1985-10-29 Kallestad Laboratories, Inc. Method for ligand determination utilizing an immunoassay monitorable by biotin-containing enzymes, and compositions therefor
US4582810A (en) * 1983-09-30 1986-04-15 Becton, Dickinson And Company Immuno-agglutination particle suspensions
US4752638A (en) * 1983-11-10 1988-06-21 Genetic Systems Corporation Synthesis and use of polymers containing integral binding-pair members
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DE3829245A1 (de) * 1988-08-29 1990-03-01 Boehringer Mannheim Gmbh Verfahren zur bestimmung einer spezifisch bindefaehigen substanz

Also Published As

Publication number Publication date
EP0397113A2 (de) 1990-11-14
JPH07117536B2 (ja) 1995-12-18
US5212063A (en) 1993-05-18
ATE97496T1 (de) 1993-12-15
DE59003503D1 (de) 1993-12-23
JPH02309254A (ja) 1990-12-25
EP0397113B1 (de) 1993-11-18
DE3915135A1 (de) 1990-11-15
EP0397113A3 (de) 1991-05-29
DK0397113T3 (da) 1994-02-07

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