ES2034245T3 - Determinacion de concentraciones ambiente de varios analitos. - Google Patents

Determinacion de concentraciones ambiente de varios analitos.

Info

Publication number
ES2034245T3
ES2034245T3 ES8888307273T ES88307273T ES2034245T3 ES 2034245 T3 ES2034245 T3 ES 2034245T3 ES 8888307273 T ES8888307273 T ES 8888307273T ES 88307273 T ES88307273 T ES 88307273T ES 2034245 T3 ES2034245 T3 ES 2034245T3
Authority
ES
Spain
Prior art keywords
ligand
sample
determination
support
several analyzes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES8888307273T
Other languages
English (en)
Inventor
Roger Philip Ekins
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Multilyte Ltd
Original Assignee
Multilyte Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=10631410&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2034245(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from PCT/GB1987/000558 external-priority patent/WO1988001058A1/en
Application filed by Multilyte Ltd filed Critical Multilyte Ltd
Application granted granted Critical
Publication of ES2034245T3 publication Critical patent/ES2034245T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • G01N33/78—Thyroid gland hormones, e.g. T3, T4, TBH, TBG or their receptors
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/973—Simultaneous determination of more than one analyte

Abstract

UN METODO PARA DETERMINAR LAS CONCENTRACIONES AMBIENTES DE UNA DIVERSIDAD DE ANALITOS EN UNA MUESTRA LIQUIDA DE V LITROS DE VOLUMEN, COMPRENDE CARGAR DIVERSOS AGENTES DE ENLACE DIFERENTES, SIENDO CAPAZ CADA UNO DE ENLAZAR REVERSIBLEMENTE UN ANALITO QUE ESTA O PUEDE ESTAR PRESENTE EN LA MUESTRA LIQUIDA Y ES ESPECIFICO DEL ANALITO EN COMPARACION CON OTROS ANALITOS DE LA MUESTRA LIQUIDA, EN UN MEDIO SOPORTE, EN UNA DIVERSIDAD DE POSICIONES SEPARADAS, TALES QUE CADA POSICION TIENE NO MAS QUE 0,1 V/K, PREFERIBLEMENTE MENOS QUE 0,01 V/K, MOLES DE UN AGENTE ENLAZANTE SENCILLO, DONDE K LITROS POR MOL ES LA CONSTANTE DE EQUILIBRIO DEL AGENTE ENLAZANTE DEL ANALITO; PONER EN CONTACTO EL MEDIO SOPORTE CARGADO CON LA MUESTRA LIQUIDA QUE SE ANALIZA, DE MODO QUE CADA POSICION SEPARADA ESTE EN CONTACTO EN LA MISMA OPERACION CON LA MUESTRA LIQUIDA, SIENDO LA CANTIDAD DE LIQUIDO USADO EN LA MUESTRA TAL QUE SOLO UNA PROPORCION INSIGNIFICANTE DEL ANALITO PRESENTE EN LA MUESTRA LIQUIDA SE ENLAZA AL AGENTE ENLAZANTE ESPECIFICO; Y MEDIR UN PARAMETRO REPRESENTATIVO DE LA OCUPACION FRACCIONADA POR LOS ANALITOS DE LOS AGENTES ENLAZANTES EN LAS POSICIONES SEPARADAS MEDIANTE UNA TECNICA DE ENSAYO COMPETITIVA O NO COMPETITIVA, UTILIZANDO UN REACTIVO DE RECONOCIMIENTO DE POSICION PARA CADA AGENTE ENLAZANTE CAPAZ DE RECONOCER, YA LAS POSICIONES DE ENLACE NO LLENAS, O LAS POSICIONES DE ENLACE LLENAS EN EL AGENTE ENLAZANTE, SIENDO MARCADO DICHO REACTIVO DE RECONOCIMIENTO DE POSICIONES CON UN MARCADOR QUE REPRESENTA LA CANTIDAD DE DICHO REACTIVO EN LA POSICION PARTICULAR QUE SE MIDE. TAMBIEN SE PROPORCIONA UN DISPOSITIVO Y EQUIPO PARA UTILIZAR EN EL METODO.
ES8888307273T 1987-08-06 1988-08-05 Determinacion de concentraciones ambiente de varios analitos. Expired - Lifetime ES2034245T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
PCT/GB1987/000558 WO1988001058A1 (en) 1986-08-06 1987-08-06 Determination of analyte concentration using two labelling markers
GB888803000A GB8803000D0 (en) 1988-02-10 1988-02-10 Determination of ambient concentrations of several analytes

Publications (1)

Publication Number Publication Date
ES2034245T3 true ES2034245T3 (es) 1993-04-01

Family

ID=10631410

Family Applications (1)

Application Number Title Priority Date Filing Date
ES8888307273T Expired - Lifetime ES2034245T3 (es) 1987-08-06 1988-08-05 Determinacion de concentraciones ambiente de varios analitos.

Country Status (18)

Country Link
US (1) US5432099A (es)
EP (2) EP0304202B1 (es)
JP (1) JP2562194B2 (es)
KR (1) KR970007079B1 (es)
AT (1) ATE78101T1 (es)
AU (1) AU625052B2 (es)
BR (1) BR8807644A (es)
CA (1) CA1334278C (es)
DE (1) DE3872621T2 (es)
DK (1) DK164944C (es)
ES (1) ES2034245T3 (es)
FI (1) FI92110C (es)
GB (1) GB8803000D0 (es)
GR (1) GR3005905T3 (es)
HU (1) HU884720D0 (es)
NO (1) NO177205C (es)
WO (1) WO1989001157A1 (es)
ZA (1) ZA885825B (es)

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Also Published As

Publication number Publication date
GR3005905T3 (es) 1993-06-07
CA1334278C (en) 1995-02-07
EP0304202B1 (en) 1992-07-08
ATE78101T1 (de) 1992-07-15
NO177205B (no) 1995-04-24
BR8807644A (pt) 1990-08-07
EP0304202A1 (en) 1989-02-22
EP0375700A1 (en) 1990-07-04
FI92110C (fi) 1994-09-26
DK29390A (da) 1990-04-05
FI900557A0 (fi) 1990-02-05
US5432099A (en) 1995-07-11
DK164944C (da) 1993-02-01
AU625052B2 (en) 1992-07-02
KR890702033A (ko) 1989-12-22
DK164944B (da) 1992-09-14
FI92110B (fi) 1994-06-15
NO177205C (no) 1995-08-02
DK29390D0 (da) 1990-02-05
DE3872621D1 (es) 1992-08-13
NO900534L (no) 1990-02-05
JP2562194B2 (ja) 1996-12-11
JPH03503081A (ja) 1991-07-11
NO900534D0 (no) 1990-02-05
ZA885825B (en) 1989-04-26
AU2253488A (en) 1989-03-01
HU884720D0 (en) 1990-09-28
DE3872621T2 (de) 1993-02-18
GB8803000D0 (en) 1988-03-09
KR970007079B1 (ko) 1997-05-02
WO1989001157A1 (en) 1989-02-09

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