ES2031818T3 - Plasmido de analisis y clonacion de regiones reguladoras para bacilos. - Google Patents

Plasmido de analisis y clonacion de regiones reguladoras para bacilos.

Info

Publication number
ES2031818T3
ES2031818T3 ES198686201951T ES86201951T ES2031818T3 ES 2031818 T3 ES2031818 T3 ES 2031818T3 ES 198686201951 T ES198686201951 T ES 198686201951T ES 86201951 T ES86201951 T ES 86201951T ES 2031818 T3 ES2031818 T3 ES 2031818T3
Authority
ES
Spain
Prior art keywords
regions
regulatory regions
baciles
analysis
plasmids
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES198686201951T
Other languages
English (en)
Inventor
Jan Hendrik Van Ee
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gist Brocades NV
Original Assignee
Gist Brocades NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gist Brocades NV filed Critical Gist Brocades NV
Application granted granted Critical
Publication of ES2031818T3 publication Critical patent/ES2031818T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/832—Bacillus

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

COMPRENDEN NUEVAS CONSTRUCCIONES EN PLASMIDOS SUMINISTRADAS PARA LA EVALUACION DE LA EFICIENCIA DE EXPRESION Y SECRECION DE GENES ESTRUCTURALES. LAS CONSTRUCCIONES SUMINISTRAN PARA LOS ESPACIOS REGULADORES TRANSCRIPCIONES Y TRASLACIONES, Y UN GEN ESTRUCTURAL Y DE SECUENCIA DE SEÑAL, QUE TAMBIEN PUEDE SER EXCINDIDA Y SUSTITUIDA DE TAL FORMA QUE PERMITE LA MEZCLA E IGUALACION DE LOS ESPACIOS REGULADORES, SECUENCIAS DE SEÑAL Y GENES PARA EVALUAR LOS ESPACIOS PARA EL USO EN LA EXPRESION DE LOS PEPTIDOS DESEADOS; LOS PLASMIDOS SE SUMINISTRAN PARA INVESTIGAR SECUENCIAS DE BACILOS PARA ESPACIOS DE REGULACION ESPECIALMENTE PROMOTORES QUE EMPLEAN UN GEN ESTRUCTURAL QUE SEGREGA UNA ENZIMA QUE PUEDE PRODUCIR UN PRODUCTO QUE PERMITE LA DETECCION VISUAL.
ES198686201951T 1985-11-08 1986-11-10 Plasmido de analisis y clonacion de regiones reguladoras para bacilos. Expired - Lifetime ES2031818T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
NL8503074 1985-11-08

Publications (1)

Publication Number Publication Date
ES2031818T3 true ES2031818T3 (es) 1993-01-01

Family

ID=19846830

Family Applications (1)

Application Number Title Priority Date Filing Date
ES198686201951T Expired - Lifetime ES2031818T3 (es) 1985-11-08 1986-11-10 Plasmido de analisis y clonacion de regiones reguladoras para bacilos.

Country Status (9)

Country Link
US (1) US5024943A (es)
EP (1) EP0224294B1 (es)
JP (1) JPS62181789A (es)
AT (1) ATE56989T1 (es)
DE (1) DE3674548D1 (es)
DK (1) DK533286A (es)
ES (1) ES2031818T3 (es)
FI (1) FI864547A (es)
PT (1) PT83714B (es)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5278066A (en) * 1985-08-09 1994-01-11 Gist-Brocades Nv Molecular cloning and expression of gene encoding lipolytic enzyme
US6238889B1 (en) 1986-12-16 2001-05-29 Dsm N.V. Molecular cloning and expression of the Pro8 isoform of human IL-3
US5830735A (en) * 1987-03-06 1998-11-03 Gist-Brocades Nv Method for producing lipolytic enzymes using transformed Pseudomonas
US5304637A (en) * 1987-07-13 1994-04-19 Gist-Brocades N.V. Expression and purification of human interleukin-3 and muteins thereof
US6384194B1 (en) 1987-12-16 2002-05-07 Dsm N.V. Expression and purification of human interleukin-3 and muteins thereof
EP0424473B1 (en) * 1988-07-05 1996-05-08 Baylor College Of Medicine Methods for identification of bacteria
ES2117625T3 (es) * 1989-06-29 1998-08-16 Genencor Int Alfa-amilasas microbianas mutantes con estabilidad termica, acida y/o alcalina incrementada.
US8232081B2 (en) 1999-09-21 2012-07-31 Basf Se Methods and microorganisms for production of panto-compounds
EP1214420B1 (en) * 1999-09-21 2016-03-23 Basf Se Methods and microorganisms for production of panto-compounds
CN102112621A (zh) * 2008-06-06 2011-06-29 丹尼斯科美国公司 用来自枯草芽孢杆菌的α-淀粉酶从淀粉产生葡萄糖
BRPI0915606A2 (pt) * 2008-06-06 2021-03-09 Danisco Us Inc. Alfa-amilases variantes de bacillus subtilis e métodos de uso das mesmas
WO2009149283A1 (en) * 2008-06-06 2009-12-10 Danisco Us Inc. Saccharification enzyme composition
US8871441B2 (en) * 2008-08-21 2014-10-28 Novozymes A/S Microfluidic device screening method
CA2743047A1 (en) 2008-11-11 2010-05-20 Danisco Us Inc. Compositions and methods comprising serine protease variants
CN103122345A (zh) 2008-11-11 2013-05-29 丹尼斯科美国公司 包含一种或多种可组合的突变的芽孢杆菌属枯草杆菌蛋白酶
CA2743060C (en) 2008-11-11 2017-03-07 Danisco Us Inc. Compositions and methods comprising a subtilisin variant
CN101434308B (zh) * 2008-11-12 2010-08-25 中材科技股份有限公司 桶
CA2778471A1 (en) 2009-10-23 2011-04-28 Danisco Us Inc. Methods for reducing blue saccharide

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2048894A (en) * 1979-05-11 1980-12-17 Gist Brocades Nv Plasmid
US4711843A (en) * 1980-12-31 1987-12-08 Cetus Corporation Method and vector organism for controlled accumulation of cloned heterologous gene products in Bacillus subtilis
FI64813C (fi) * 1980-12-31 1984-01-10 Ilkka Antero Palva Foerfarande foer producering av ett utvalt aeggviteaemne och vid foerfarandet anvaenda rekombinantplasmidvektorer
US4624926A (en) * 1981-01-02 1986-11-25 The Research Foundation Of State University Of New York Novel cloning vehicles for polypeptide expression in microbial hosts
DE3480411D1 (en) * 1983-07-06 1989-12-14 Gist Brocades Nv Molecular cloning and expression in industrial microorganism species

Also Published As

Publication number Publication date
EP0224294A1 (en) 1987-06-03
DE3674548D1 (de) 1990-10-31
ATE56989T1 (de) 1990-10-15
DK533286D0 (da) 1986-11-07
PT83714A (en) 1986-12-01
FI864547A (fi) 1987-05-09
US5024943A (en) 1991-06-18
PT83714B (pt) 1988-08-17
JPS62181789A (ja) 1987-08-10
DK533286A (da) 1987-05-09
FI864547A0 (fi) 1986-11-07
EP0224294B1 (en) 1990-09-26

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