ES2053818T3 - Vectores de expresion para la sintesis de proteinas y replicones de plasmidos y cassettes de secuencias para utilizarse en la construccion de tales vectores. - Google Patents

Vectores de expresion para la sintesis de proteinas y replicones de plasmidos y cassettes de secuencias para utilizarse en la construccion de tales vectores.

Info

Publication number
ES2053818T3
ES2053818T3 ES88906974T ES88906974T ES2053818T3 ES 2053818 T3 ES2053818 T3 ES 2053818T3 ES 88906974 T ES88906974 T ES 88906974T ES 88906974 T ES88906974 T ES 88906974T ES 2053818 T3 ES2053818 T3 ES 2053818T3
Authority
ES
Spain
Prior art keywords
sequences
vectors
proteins
expression
expression vectors
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES88906974T
Other languages
English (en)
Inventor
David Hugh Langler Bishop
Vincent Clive Emry
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Natural Environmental Research Council
Original Assignee
Natural Environmental Research Council
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB878719108A external-priority patent/GB8719108D0/en
Priority claimed from GB888816084A external-priority patent/GB8816084D0/en
Application filed by Natural Environmental Research Council filed Critical Natural Environmental Research Council
Application granted granted Critical
Publication of ES2053818T3 publication Critical patent/ES2053818T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/14011—Baculoviridae
    • C12N2710/14111—Nucleopolyhedrovirus, e.g. autographa californica nucleopolyhedrovirus
    • C12N2710/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2710/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

Abstract

Un replicón de plasmidos para emplearse en la introducción de una pluralidad de genes en un vector de expresión, comprendiendo dicho replicón de plasmidos DNA de doble hebra que contiene las secuencias (a), (b1), (b2) y (c) como a continuación se definen:(a) una secuencia que permite reproducir el replicón en un hospedante bacterial; (b1 y b2) una primera y una segunda secuencias adaptadas para permitir situar una secuencia interviniente entre dichas primera y segunda secuencias que han de introducirse en un vector de expresión; y (c) una secuencia interviniente situada entre dichas y primera y segunda secuencias (b1) y (b2); caracterizado porque la secuencia interviniente (c) comprende una primera y una segunda secuencias de expresión de polipéptidos (PESs) designadas por (c1) y (c2) en donde dicha primera PES (c1) incluye (I) un primer promotor transcripcional, (II) un primer punto de restricción único para la introducción en primer lugar de un primer gen que es natural o foráneo al vector de expresión, encontrándose dicho primer gen bajo el control de dicho primer promotor transcripcional, y (III) un primer punto de terminación transcripcional; y en donde dicha segunda PES (c2) incluye (IV) un segundo promotor transcripcional, (V) un segundo punto de restricción único para la introducción de un segundo gen que es natural o foráneo al vector de expresión, encontrándose dicho segundo gen bajo el control de dicho segundo promotor transcripcional, y (VI) un segundo punto de terminación transcripcional.
ES88906974T 1987-08-12 1988-08-11 Vectores de expresion para la sintesis de proteinas y replicones de plasmidos y cassettes de secuencias para utilizarse en la construccion de tales vectores. Expired - Lifetime ES2053818T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB878719108A GB8719108D0 (en) 1987-08-12 1987-08-12 Expression vectors
GB888816084A GB8816084D0 (en) 1988-07-12 1988-07-12 Expression vectors for synthesis of proteins & plasmid replicons & sequence cassettes for use in constructing such vectors

Publications (1)

Publication Number Publication Date
ES2053818T3 true ES2053818T3 (es) 1994-08-01

Family

ID=26292601

Family Applications (1)

Application Number Title Priority Date Filing Date
ES88906974T Expired - Lifetime ES2053818T3 (es) 1987-08-12 1988-08-11 Vectores de expresion para la sintesis de proteinas y replicones de plasmidos y cassettes de secuencias para utilizarse en la construccion de tales vectores.

Country Status (10)

Country Link
EP (2) EP0305087A1 (es)
JP (1) JPH02501114A (es)
CN (1) CN1034757A (es)
AT (1) ATE88499T1 (es)
AU (1) AU628788B2 (es)
DE (1) DE3880473T2 (es)
ES (1) ES2053818T3 (es)
GR (1) GR3007687T3 (es)
IL (1) IL87394A0 (es)
WO (1) WO1989001518A1 (es)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5876962A (en) * 1987-08-12 1999-03-02 Natural Environment Research Council Expression vectors for the synthesis of proteins and plasmid replicons and sequence cassettes for use in constructing such vectors
US5686270A (en) * 1989-07-07 1997-11-11 Oravax, Inc. Production of antigens by self-assembly of polypeptide components
US5162222A (en) * 1989-07-07 1992-11-10 Guarino Linda A Use of baculovirus early promoters for expression of foreign genes in stably transformed insect cells or recombinant baculoviruses
GB8915572D0 (en) * 1989-07-07 1989-08-23 Natural Environment Res Use of bluetongue virus proteins as vaccine components
GB8915571D0 (en) * 1989-07-07 1989-08-23 Natural Environment Res Production of antigens by self-assembly of polypeptide components
US5169784A (en) * 1990-09-17 1992-12-08 The Texas A & M University System Baculovirus dual promoter expression vector
GB9106185D0 (en) * 1991-03-22 1991-05-08 Wellcome Found Biological control agents
AU4248297A (en) * 1996-09-03 1998-03-26 General Hospital Corporation, The Inhibition of viral replication
DE69831417T2 (de) 1997-09-23 2006-06-22 Genvec, Inc. Duale selektionkassette und sie enthaltende plasmide

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0275300B1 (en) * 1986-08-01 1996-01-03 Commonwealth Scientific And Industrial Research Organisation Recombinant vaccine

Also Published As

Publication number Publication date
AU628788B2 (en) 1992-09-24
DE3880473D1 (de) 1993-05-27
GR3007687T3 (es) 1993-08-31
EP0327626B1 (en) 1993-04-21
EP0327626A1 (en) 1989-08-16
WO1989001518A1 (en) 1989-02-23
ATE88499T1 (de) 1993-05-15
DE3880473T2 (de) 1993-08-05
JPH02501114A (ja) 1990-04-19
EP0305087A1 (en) 1989-03-01
IL87394A0 (en) 1989-12-15
AU2253288A (en) 1989-03-09
CN1034757A (zh) 1989-08-16

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