EP4043551A1 - Nährmedien mit pflanzenproteinhydrolysaten für zellkultur - Google Patents

Nährmedien mit pflanzenproteinhydrolysaten für zellkultur Download PDF

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Publication number
EP4043551A1
EP4043551A1 EP21156945.4A EP21156945A EP4043551A1 EP 4043551 A1 EP4043551 A1 EP 4043551A1 EP 21156945 A EP21156945 A EP 21156945A EP 4043551 A1 EP4043551 A1 EP 4043551A1
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EP
European Patent Office
Prior art keywords
plant
hydrolysate
acid
dry matter
bran
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
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EP21156945.4A
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English (en)
French (fr)
Inventor
Frank CORDESMEYER
Jay O'NIEN
Beatrice Conde-Petit
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Buehler AG
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Buehler AG
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Filing date
Publication date
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Priority to EP21156945.4A priority Critical patent/EP4043551A1/de
Priority to IL304952A priority patent/IL304952A/en
Priority to CN202280013969.5A priority patent/CN116829694A/zh
Priority to AU2022219484A priority patent/AU2022219484A1/en
Priority to CA3206235A priority patent/CA3206235A1/en
Priority to KR1020237028436A priority patent/KR20230135123A/ko
Priority to PCT/EP2022/053163 priority patent/WO2022171696A1/en
Priority to US18/276,886 priority patent/US20240043795A1/en
Publication of EP4043551A1 publication Critical patent/EP4043551A1/de
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0043—Medium free of human- or animal-derived components
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
    • A23J1/001—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from waste materials, e.g. kitchen waste
    • A23J1/005—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from waste materials, e.g. kitchen waste from vegetable waste materials
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
    • A23J1/006—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from vegetable materials
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
    • A23J1/12—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from cereals, wheat, bran, or molasses
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
    • A23J1/14—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J3/00—Working-up of proteins for foodstuffs
    • A23J3/14—Vegetable proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J3/00—Working-up of proteins for foodstuffs
    • A23J3/14—Vegetable proteins
    • A23J3/18—Vegetable proteins from wheat
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J3/00—Working-up of proteins for foodstuffs
    • A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
    • A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/70—Undefined extracts
    • C12N2500/76—Undefined extracts from plants

Definitions

  • the present invention relates to nutritional components suitable for cell culture of eukaryotic cells. These nutritional components can be prepared from plant-based protein hydrolysates.
  • the invention further relates to nutrient media comprising these nutritional components.
  • the nutrient media of the invention can be prepared by replacing serum components, which are common in such media, - at least partially - by the nutritional components of the invention.
  • the nutritional components and the nutrient media of the invention are particularly suitable for use in the food industry, such as in the production of cultured meat.
  • WO 98/15614 A1 describes cell culture medium formulations for the in vitro cultivation of animal cells. These media formulations comprise at least one plant-derived peptide and/or at least one plant-derived lipid and/or at least one plant-derived fatty acid. Rice, soy, potato, and corn are named as suitable sources of proteins, peptides, lipids, and/or fatty acids. Wheat is specifically excluded as a source of plant-derived proteins.
  • the plant peptides to be used in the formulations of WO 98/15614 A1 may be prepared by digesting plant extracts with enzymes such as trypsin or chymotrypsin. The plant peptides are to be added to the basal medium at concentrations of about 10 - 1000 mg/liter.
  • WO 03/045995 A2 describes the cost-effective production of recombinant glycoproteins such as human erythropoietin using a serum-free culture medium.
  • the culture medium comprises a plant-derived peptone.
  • the examples of WO 03/045995 A2 show a serum-free adaptation medium and a serum-free production medium. Each one of these two media contains 0.25% soya-peptone.
  • US 9,714,411 B2 describes animal protein-free cell culture media comprising polyamines and a plant- and/or yeast-derived hydrolysate.
  • the total concentration of the plant- and/or yeast-derived protein hydrolysate in the cell culture medium is about 0.05% to about 5% (w/v).
  • the plant-derived protein hydrolysate may be selected from a cereal hydrolysate and/or a soy hydrolysate.
  • US 9,714,411 B2 defines the term "hydrolysate" as relating to any enzymatic digest of a vegetable or yeast extract.
  • JP2013-247927 A discloses that a rice bran extract has a cell growth promoting action, when used in cell culture medium.
  • the rice bran extract preferably comprises 50% by mass or more of protein.
  • the rice bran extract may be a molecular weight fractionated product, preferably in the range between 3 kDa and 10kDa.
  • JP2013-247927 A it was shown that a rice bran extract with a protein content of 55% improved proliferation of CHO cells when used in concentrations of 0.1 or 0.3 g/L, while cytotoxicity was observed at a concentration of 1 g/L.
  • nutrient media for cell culture are a mixture of about 20 to 60 high grade and pure ingredients. The high number and the high purity of the ingredients renders such nutrient media very expensive.
  • plant-based hydrolysates were used as additives for cell culture media.
  • the plant-based extracts used in the prior art were obtained from isolated plant proteins or from protein-rich plant material, which can be grouped as primary food stream material.
  • nutrient media components can be prepared using plant-based protein hydrolysates that were produced from secondary food stream material, i.e. material having lower economic value than primary food stream material.
  • novel composition containing plant-based hydrolysates prepared from secondary food stream are much cheaper than the media ingredients of the prior art.
  • These novel compositions can be used as media components and support the growth of eukaryotic cells, and can even be used in the production of cultured meat.
  • the components and compositions of the present invention can be used for cell cultivation in an industrial scale.
  • the present invention relates to a composition
  • a composition comprising:
  • the present invention relates to a use of the composition according to the first aspect in the production of cultured meat.
  • the present invention relates to a method for the production of a plant-based hydrolysate obtained from secondary food stream material, comprising the steps:
  • the present invention relates to a plant-based hydrolysate obtainable by the method of the third aspect.
  • the present invention relates to a method for the production of the composition of the first aspect, comprising the step(s):
  • the expression material from "primary food stream” refers to plant material that is suitable for human consumption. This material is characterized by high protein content (at least 30% (w/w dry matter) but typically at least 50% (w/w dry matter)) and low fibre content (5% or less (w/w dry matter), but typically 1% or less (w/w dry matter)).
  • primary food stream material include, without limitation, protein-rich material derived from soy, fava bean, wheat (e.g. wheat gluten), rice, pea, potato, or cotton seed.
  • secondary food stream refers to plant material that is generally not used for human consumption. Material from secondary food stream is mostly used as animal feed, as fertilizer or is simply burnt. As compared to material from primary food stream, secondary food stream material has a lower protein content (4% to 35%, and typically 10% to 30% (w/w dry matter)) and a higher fibre content (1% to 50%, and typically 10% to 20% (w/w dry matter)). Examples of secondary food stream material include, without limitation, rice bran, defatted rice bran, wheat bran, rye bran, maize bran, spelt bran, or brewer's spent grain.
  • a “growth factor” refers to a protein or a peptide that promotes proliferation of eukaryotic cells in cell culture.
  • growth factors usable in the present invention include, without limitation insulin and insulin-like growth factors (IGF).
  • a protein content of "about 10% (w / w dry matter)" encompasses a protein content ranging from 9.5% (w/w dry matter) to about 10.5% (w/w dry matter).
  • a temperature of "about 80°C” refers to a temperature range between 76°C and 84°C.
  • the present invention is directed to a composition
  • a composition comprising:
  • the secondary food stream material has a protein content ranging from about 5% to about 34%; preferably from about 6% to about 33%, preferably from about 7% to about 32%, preferably from about 8% to about 31%, preferably from about 9% to about 30%, preferably from about 10% to about 29%, preferably from about 11% to about 28%, preferably from about 12% to about 27%, preferably from about 13% to about 26%, preferably from about 14% to about 25%, and most preferably from about 15% to about 25% (w/w dry matter).
  • the secondary food stream material has a fibre content ranging from about 2% to about 45%, preferably from about 3% to about 40%, preferably from about 4% to about 35%, preferably from about 5% to about 30%, and most preferably from about 6% to about 25% (w/w dry matter).
  • the secondary food stream material is or is prepared from rice bran, wheat bran, rye bran, maize bran, spelt bran, or brewer's spent grain.
  • the secondary food stream material is or is prepared from
  • the first plant-based hydrolysate is present in the composition in a concentration in the range from 0.1% to 50% (w/w).
  • the food grade premix is present in the composition in a concentration in the range from 10% to 99.9% (w/w).
  • composition further comprises:
  • the primary food stream material has a protein content of at least 32%, preferably at least 34%, preferably at least 36%, preferably at least 38%, preferably at least 40%, preferably at least 42%, preferably at least 44%, preferably at least 46%, preferably at least 48, and most preferably at least 50% (w/w dry matter).
  • the primary food stream material has a fibre content of 4% (w/w dry matter) or less, preferably 3% (w/w dry matter) or less, preferably 2% (w/w dry matter) or less, and most preferably 1% (w/w dry matter) or less.
  • the second plant-based hydrolysate prepared from primary food stream material has been prepared from soy bean, fava bean, wheat, rice, pea, potato, or cotton seed.
  • the second plant-based hydrolysate obtained from primary food stream is present in a concentration in the range from 0.1% to 50% (w/w).
  • the second plant-based hydrolysate obtained from primary food stream was prepared by applying the method of the third aspect. This means that a plant-based material obtained from primary food stream, as defined above, is provided and that then steps (b) to (g) of the method of the third aspect are carried out.
  • the composition according to the first aspect is used as a replacement of cell culture medium.
  • An aqueous solution of the composition according to the first aspect can be prepared, thereby obtaining a plant-based medium.
  • This plant-based medium can be used to partially replace a standard cell culture medium. For example, from about 100 mL to about 500 mL (preferably from about 150 mL to about 450 mL, preferably from about 200 mL to about 400 mL, more preferably from about 250 mL to about 350 mL, even more preferably about 300 mL) are removed from 1 L of a standard cell culture medium and the same volume of the plant-based medium as the volume that was removed is added to the remaining volume of standard cell culture medium as a replacement. For example, 300 mL are removed from 1 L of a standard cell culture medium and 300 mL of the plant-based medium are added to the remaining 700 mL as a replacement.
  • the composition according to the first aspect is used as the starting material for the preparation of a cell culture medium.
  • further components salts, buffer, energy sources (e.g. glucose), trace minerals, essential amino acids, growth factors) can then be added.
  • Osmolality and pH can be adjusted.
  • a person skilled in the art will be well-aware which further components, which pH and which osmolality will be suitable to promote growth of eukaryotic cells.
  • the present invention is directed to a use of the composition according to the first aspect in the production of cultured meat.
  • a method for the production of cultured meat comprising the steps:
  • the sterile aqueous solvent is selected from the group consisting of water, phosphate buffer, and a solution of sodium chloride.
  • the appropriate cells are selected from the group consisting of myosatellite cells, mesenchymal stromal cells, fibroblast cells, muscle cells, stem cells, and induced pluripotent stem cells (iPSCs).
  • the appropriate cells are bovine cells, porcine cells or chicken cells.
  • stem cells are human embryonic stem cells
  • these human embryonic stem cells were prepared by a process that does not require the destruction of the human embryo.
  • a person skilled in the art of producing cultured meat will be well aware which temperature ranges and which time-periods are required for obtaining cultured meat, taking into consideration the type of cells and the amount of cells used for inoculation.
  • the present invention is directed to a method for the production of a plant-based hydrolysate obtained from secondary food stream, comprising the steps:
  • the plant-based material obtained from secondary food stream has a protein content ranging from about 5% to about 34%; preferably from about 6% to about 33%, preferably from about 7% to about 32%, preferably from about 8% to about 31%, preferably from about 9% to about 30%, preferably from about 10% to about 29%, preferably from about 11% to about 28%, preferably from about 12% to about 27%, preferably from about 13% to about 26%, preferably from about 14% to about 25%, and most preferably from about 15% to about 25% (w/w dry matter).
  • the plant-based material obtained from secondary food stream has a fibre content ranging from about 2% to about 45%, preferably from about 3% to about 40%, preferably from about 4% to about 35%, preferably from about 5% to about 30%, and most preferably from about 6% to about 25% (w/w dry matter).
  • the plant-based material obtained from secondary food stream is or is obtained from rice bran, wheat bran, rye bran, maize bran, spelt bran, or brewer's spent grain.
  • the plant-based material obtained from secondary food stream is or is prepared from
  • the acid added in step (b) is a concentrated solution of HCl.
  • the concentration of HCl is in the range from 0.1 M to 2 M, more preferably in the range from 0.2 M to 1.5 M, more preferably in the range from 0.3 M to 1 M, even more preferably in the range from 0.4 M to about 0.75 M, and most preferably at about 0.5 M.
  • the incubation of step (c) is carried out at a temperature in the range between 61°C and 99°C, preferably at a temperature in the range between 62°C and 98°C, preferably at a temperature in the range between 63°C and 97°C, preferably at a temperature in the range between 64°C and 96°C, preferably at a temperature in the range between 65°C and 95°C, preferably at a temperature in the range between 66°C and 94°C, preferably at a temperature in the range between 67°C and 93°C, preferably at a temperature in the range between 68°C and 92°C, preferably at a temperature in the range between 69°C and 91°C, preferably at a temperature in the range between 70°C and 90°C, preferably at a temperature in the range between 71°C and 89°C, preferably at a temperature in the range between 72°C and 88°C, preferably
  • step (c) is carried out for a time period between 12 hours and 48 hours, preferably between 15 hours and 36 hours, more preferably between 18 hours and 30 hours, and most preferably for about 24 hours.
  • step (e) is carried out for about 5 min to about 20 min at about 4000 to 6000 x g, preferably for about 10 min to 15 min at about 4500 to about 5500 x g; most preferably for about 10 min at about 5250 x g.
  • the sterile filter is a filter with a pore size of 50 ⁇ m or less, preferably 20 ⁇ m or less, more preferably 10 ⁇ m or less, and even more preferably, 0.2 ⁇ m or less.
  • filtration steps with increasingly smaller pore sizes are used; e.g. a first filtration step with a filter having a pore size of 50 ⁇ m or less, a second filtration step with a filter having a pore size of 20 ⁇ m or less, a third filtration step with a filter having a pore size of 10 ⁇ m or less, and a fourth filtration step with a filter having a pore size of 0.2 ⁇ m or less.
  • the method of the third aspect and its preferred embodiments are also suitable for producing a plant-based hydrolysate obtained from primary food stream when using a plant-based material obtained from primary food stream as starting material.
  • the present invention is directed to a plant-based hydrolysate obtainable by the method of the third aspect.
  • the plant-based hydrolysate has been obtained by the method of the third aspect.
  • the present invention is directed to a method for the production of the composition of the first aspect, comprising the step(s):
  • the plant-based hydrolysate prepared from secondary food stream is obtainable by the method of the third aspect.
  • the plant-based hydrolysate prepared from secondary food stream has been obtained by the method of the third aspect.
  • the secondary food stream material is as defined in the embodiments of the first aspect.
  • the food grade premix is defined as in the embodiments of the first aspect of the invention.
  • the primary food stream material is as defined in the embodiments of the first aspect.
  • Fig. 1 shows a cell culture experiment with CHO cells.
  • CHO cells were grown in reference medium or in media, in which components of the reference medium were replaced by a hydrolysate of defatted rice bran (RBD) (20% protein content). Two different lots of RBD hydrolysate were tested. At each day of cultivation, viable cell density (in 10 6 cells/mL) and viability of cells were recorded for each experiment.
  • RBD defatted rice bran
  • the plant material (either from primary food stream or from secondary food stream) was hydrolysed using the following protocol:
  • Plant hydrolysates were prepared from the following starting materials (protein content is indicated in brackets): rye bran (17.1%), wheat bran (14.3%), fava bean (66%), spelt bran (19.7%), legria (21.2%), stabilized rice bran (15.1%), and defatted rice bran (19.0%).
  • CHO-K1 cells Chinese hamster ovary cell line, subclone K1 Reference medium Liquid SBF medium, obtainable from Xell AG (Bielefeld, Germany) upon request under the product number 2052-0001
  • CHO-K1 cells were grown in reference medium or in a medium containing a replacement with a hydrolysate of defatted rice bran (RBD) (20% protein content).
  • RBD defatted rice bran
  • the hydrolysates of defatted rice bran had been prepared according to the acid hydrolysis protocol described in Example 1 above. Two different lots of RBD hydrolysate were tested.
  • Reference medium 1:3 means that 300 mL per 1 L of reference medium were replaced by distilled water.
  • RBD and RBD1 both refer to a medium composition, in which 300 mL of defatted rice bran medium were added to 700 mL reference medium.
  • Glucose concentration in all media variants was adjusted to either 4.5 g/L (for RBD and RBD1) or 6.2 g/L (for Reference 1:3), depending of glucose concentration of the food grade materials used.
  • Glutamine was added to a final concentration of 6 mM.
  • Cells were cultivated in 125 mL plain shake flasks with a working volume of 50 mL (in growth curves) in a 37°C incubator with an atmosphere of 5 % CO 2 .
  • the maximum cell density was lower when using a medium containing a replacement of rice bran hydrolysate (about 4 x 10 6 cells/mL for the first lot; about 3.5 x 10 6 cells/mL for the second lot) as compared to the reference medium (5 x 10 6 cells/mL).
  • maximum cell density was reached only after longer cultivation times (at day 7 for the first lot; at day 8 for the second lot) as compared to the reference medium (at day 6).
  • the above results provide a proof of principle that a hydrolysate from rice bran (i.e. from a secondary food stream material) can be used as a supplement in cell culture media and supports the growth of eukaryotic cells.

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EP21156945.4A 2021-02-12 2021-02-12 Nährmedien mit pflanzenproteinhydrolysaten für zellkultur Pending EP4043551A1 (de)

Priority Applications (8)

Application Number Priority Date Filing Date Title
EP21156945.4A EP4043551A1 (de) 2021-02-12 2021-02-12 Nährmedien mit pflanzenproteinhydrolysaten für zellkultur
IL304952A IL304952A (en) 2021-02-12 2022-02-09 Nutrient media for cell culture that includes plant protein hydrolysates
CN202280013969.5A CN116829694A (zh) 2021-02-12 2022-02-09 用于细胞培养的包含植物蛋白水解物的营养培养基
AU2022219484A AU2022219484A1 (en) 2021-02-12 2022-02-09 Nutrient media for cell culture containing plant protein hydrolysates
CA3206235A CA3206235A1 (en) 2021-02-12 2022-02-09 Nutrient media for cell culture containing plant protein hydrolysates
KR1020237028436A KR20230135123A (ko) 2021-02-12 2022-02-09 식물 단백질 가수분해물을 함유하는 세포 배양용 영양배지
PCT/EP2022/053163 WO2022171696A1 (en) 2021-02-12 2022-02-09 Nutrient media for cell culture containing plant protein hydrolysates
US18/276,886 US20240043795A1 (en) 2021-02-12 2022-02-09 Nutrient media for cell culture containing plant protein hydrolysates

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EP21156945.4A EP4043551A1 (de) 2021-02-12 2021-02-12 Nährmedien mit pflanzenproteinhydrolysaten für zellkultur

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CN (1) CN116829694A (de)
AU (1) AU2022219484A1 (de)
CA (1) CA3206235A1 (de)
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Citations (5)

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Publication number Priority date Publication date Assignee Title
WO1998015614A1 (en) 1996-10-10 1998-04-16 Life Technologies, Inc. Animal cell culture media comprising plant-derived nutrients
WO2003045995A2 (en) 2001-11-28 2003-06-05 Sandoz Gmbh Cell culture process
US20060286668A1 (en) * 1999-04-30 2006-12-21 Invitrogen Corporation Animal-cell culture media comprising non-animal or plant-derived nutrients
JP2013247927A (ja) 2012-06-01 2013-12-12 Univ Of Fukui 米糠由来の細胞増殖促進剤
US9714411B2 (en) 2004-10-29 2017-07-25 Baxalta GmbH Animal protein-free media for cultivation of cells

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20110101782A (ko) * 2010-03-09 2011-09-16 씨제이제일제당 (주) 미강 펩톤을 포함하는 미생물 배양용 조성물

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
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