EP3950934A4 - Verfahren zur züchtung einer zellpopulation und verwendung davon - Google Patents
Verfahren zur züchtung einer zellpopulation und verwendung davon Download PDFInfo
- Publication number
- EP3950934A4 EP3950934A4 EP20778085.9A EP20778085A EP3950934A4 EP 3950934 A4 EP3950934 A4 EP 3950934A4 EP 20778085 A EP20778085 A EP 20778085A EP 3950934 A4 EP3950934 A4 EP 3950934A4
- Authority
- EP
- European Patent Office
- Prior art keywords
- cell population
- culturing cell
- culturing
- population
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000012258 culturing Methods 0.000 title 1
- 238000000034 method Methods 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0668—Mesenchymal stem cells from other natural sources
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0221—Freeze-process protecting agents, i.e. substances protecting cells from effects of the physical process, e.g. cryoprotectants, osmolarity regulators like oncotic agents
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0226—Physiologically active agents, i.e. substances affecting physiological processes of cells and tissue to be preserved, e.g. anti-oxidants or nutrients
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/32—Bones; Osteocytes; Osteoblasts; Tendons; Tenocytes; Teeth; Odontoblasts; Cartilage; Chondrocytes; Synovial membrane
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P19/00—Drugs for skeletal disorders
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/70—Undefined extracts
- C12N2500/76—Undefined extracts from plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/30—Synthetic polymers
- C12N2533/32—Polylysine, polyornithine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/50—Proteins
- C12N2533/52—Fibronectin; Laminin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/50—Proteins
- C12N2533/54—Collagen; Gelatin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Cell Biology (AREA)
- Rheumatology (AREA)
- Developmental Biology & Embryology (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Virology (AREA)
- Immunology (AREA)
- Orthopedic Medicine & Surgery (AREA)
- Environmental Sciences (AREA)
- Dentistry (AREA)
- General Chemical & Material Sciences (AREA)
- Physical Education & Sports Medicine (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Biophysics (AREA)
- Physiology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Materials For Medical Uses (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2019012571 | 2019-03-25 | ||
PCT/JP2020/013307 WO2020196615A1 (ja) | 2019-03-25 | 2020-03-25 | 細胞集団の培養方法及びその利用 |
Publications (2)
Publication Number | Publication Date |
---|---|
EP3950934A1 EP3950934A1 (de) | 2022-02-09 |
EP3950934A4 true EP3950934A4 (de) | 2022-12-28 |
Family
ID=71103931
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP20778085.9A Pending EP3950934A4 (de) | 2019-03-25 | 2020-03-25 | Verfahren zur züchtung einer zellpopulation und verwendung davon |
Country Status (11)
Country | Link |
---|---|
US (1) | US20220056418A1 (de) |
EP (1) | EP3950934A4 (de) |
JP (2) | JP6712740B1 (de) |
KR (1) | KR20210153066A (de) |
CN (1) | CN113874489A (de) |
AU (1) | AU2020244911A1 (de) |
BR (1) | BR112021019037A2 (de) |
CA (1) | CA3134256A1 (de) |
IL (1) | IL286558A (de) |
SG (1) | SG11202110461UA (de) |
WO (1) | WO2020196615A1 (de) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114591900B (zh) * | 2022-03-18 | 2022-10-18 | 河北北冥生物科技有限公司 | 一种提高干细胞分化能力的体外培养方法及培养基和应用 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20150125542A1 (en) * | 2010-12-02 | 2015-05-07 | Maruzen Pharmaceuticals Co., Ltd. | TIE2 Activator, Vascular Endothelial Growth Factor (VEGF) Inhibitor, Angiogenesis Inhibitor, Vascular Maturing Agent, Vascular Normalizing Agent and Vascular Stabilizing Agent, and Pharmaceutical Composition |
Family Cites Families (24)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5150824A (en) | 1974-10-31 | 1976-05-04 | Inoue Japax Res | Chojakukinzokutaino hyomenshorisochi |
US5252248A (en) | 1990-07-24 | 1993-10-12 | Eaton Corporation | Process for preparing a base nitridable silicon-containing material |
US6723335B1 (en) * | 2000-04-07 | 2004-04-20 | Jeffrey William Moehlenbruck | Methods and compositions for treating intervertebral disc degeneration |
JP4778629B2 (ja) * | 2001-04-24 | 2011-09-21 | 生化学工業株式会社 | コラーゲン分解促進剤 |
US20030220692A1 (en) * | 2002-02-09 | 2003-11-27 | Shapiro Irving M. | Preparations of nucleus pulposus cells and methods for their generation, identification, and use |
WO2006138720A2 (en) * | 2005-06-17 | 2006-12-28 | Sun Uk Song | Isolation of multi-lineage stem cells |
JP2009506769A (ja) * | 2005-09-02 | 2009-02-19 | エージェンシー フォー サイエンス,テクノロジー アンド リサーチ | 間充織幹細胞誘導方法 |
WO2009009020A1 (en) | 2007-07-12 | 2009-01-15 | Discgenics | Human disc tissue |
JP5126500B2 (ja) | 2007-12-26 | 2013-01-23 | いすゞ自動車株式会社 | マーキング機能付きスパナ型トルクレンチ |
ES2655487T3 (es) | 2008-03-31 | 2018-02-20 | Shiseido Company, Ltd. | Extracto de canela para tratar el fotoenvejecimiento |
JP5274431B2 (ja) | 2009-11-11 | 2013-08-28 | 株式会社 資生堂 | Tie2活性化剤、血管の成熟化、正常化又は安定化剤、リンパ管安定化剤並びにしわ防止・改善剤及びむくみ改善・予防剤 |
JP5143109B2 (ja) | 2009-11-11 | 2013-02-13 | 株式会社 資生堂 | Tie2活性化剤、血管の成熟化、正常化又は安定化剤、リンパ管安定化剤並びにしわ防止・改善剤及びむくみ改善・予防剤 |
JP2011102275A (ja) | 2009-11-11 | 2011-05-26 | Shiseido Co Ltd | Tie2活性化剤、血管の成熟化、正常化又は安定化剤、リンパ管安定化剤並びにしわ防止・改善剤及びむくみ改善・予防剤 |
JP2011102273A (ja) | 2009-11-11 | 2011-05-26 | Shiseido Co Ltd | Tie2活性化剤、血管の成熟化、正常化又は安定化剤、リンパ管安定化剤並びにしわ防止・改善剤及びむくみ改善・予防剤 |
JP2011201811A (ja) | 2010-03-25 | 2011-10-13 | Shiseido Co Ltd | Tie2活性化剤、血管の成熟化、正常化又は安定化剤、リンパ管安定化剤並びにしわ防止・改善剤及びむくみ改善・予防剤 |
JP5863639B2 (ja) | 2010-03-30 | 2016-02-16 | 学校法人東海大学 | 椎間板の状態に関する指標を得る方法、椎間板障害の治療または予防方法、および髄核細胞集団のポテンシャルまたは品質の評価方法 |
JP5926895B2 (ja) | 2011-05-11 | 2016-05-25 | 株式会社 資生堂 | Tie2活性化剤及びリンパ管安定化剤 |
CN102258809A (zh) * | 2011-07-15 | 2011-11-30 | 中国人民解放军海军总医院 | 脐带华通胶间充质干细胞在制备细胞移植材料中的应用 |
JP6293402B2 (ja) | 2012-05-18 | 2018-03-14 | 丸善製薬株式会社 | Tie2活性化剤、血管新生抑制剤、血管の成熟化剤、及び血管の正常化剤、並びに飲食品 |
JP6211380B2 (ja) | 2012-10-17 | 2017-10-11 | 丸善製薬株式会社 | Tie2活性化剤、血管新生抑制剤、血管の成熟化剤、血管の正常化剤、及び血管の安定化剤 |
US11891626B2 (en) * | 2013-03-15 | 2024-02-06 | Discgenics, Inc. | Isolated discogenic cells, methods of using, and methods of preparing same from mammalian tissue |
TWI679985B (zh) | 2014-10-16 | 2019-12-21 | 日商三得利控股股份有限公司 | 含有橄欖果實萃取物的Tie2活化劑 |
CN108697812A (zh) * | 2016-01-14 | 2018-10-23 | 脊核细胞有限责任公司 | 用于软骨细胞或软骨类型细胞再生的细胞混合物 |
CN106047801A (zh) * | 2016-05-30 | 2016-10-26 | 深圳大学 | 一种髓核细胞分离纯化方法 |
-
2019
- 2019-03-25 JP JP2019546051A patent/JP6712740B1/ja active Active
-
2020
- 2020-03-25 BR BR112021019037A patent/BR112021019037A2/pt unknown
- 2020-03-25 KR KR1020217034444A patent/KR20210153066A/ko active Search and Examination
- 2020-03-25 SG SG11202110461UA patent/SG11202110461UA/en unknown
- 2020-03-25 US US17/598,057 patent/US20220056418A1/en active Pending
- 2020-03-25 WO PCT/JP2020/013307 patent/WO2020196615A1/ja unknown
- 2020-03-25 CA CA3134256A patent/CA3134256A1/en active Pending
- 2020-03-25 JP JP2021509498A patent/JPWO2020196615A1/ja active Pending
- 2020-03-25 EP EP20778085.9A patent/EP3950934A4/de active Pending
- 2020-03-25 AU AU2020244911A patent/AU2020244911A1/en active Pending
- 2020-03-25 CN CN202080038296.XA patent/CN113874489A/zh active Pending
-
2021
- 2021-09-22 IL IL286558A patent/IL286558A/en unknown
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20150125542A1 (en) * | 2010-12-02 | 2015-05-07 | Maruzen Pharmaceuticals Co., Ltd. | TIE2 Activator, Vascular Endothelial Growth Factor (VEGF) Inhibitor, Angiogenesis Inhibitor, Vascular Maturing Agent, Vascular Normalizing Agent and Vascular Stabilizing Agent, and Pharmaceutical Composition |
Non-Patent Citations (6)
Title |
---|
CHAN SCW ET AL: "Minimizing cryopreservation-induced loss of disc cell activity for storage of whole intervertebral discs", EUROPEAN CELLS AND MATERIALS, vol. 19, 1 January 2010 (2010-01-01), pages 273 - 283, XP093000535, Retrieved from the Internet <URL:https://www.ecmjournal.org/papers/vol019/pdf/v019a26.pdf> DOI: 10.22203/eCM.v019a26 * |
LI XIAO-CHUAN ET AL: "Co-culturing nucleus pulposus mesenchymal stem cells with notochordal cell-rich nucleus pulposus explants attenuates tumor necrosis factor-[alpha]-induced senescence", STEM CELL RESEARCH & THERAPY, vol. 9, no. 1, 1 December 2018 (2018-12-01), XP093000244, Retrieved from the Internet <URL:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6019307/pdf/13287_2018_Article_919.pdf> DOI: 10.1186/s13287-018-0919-9 * |
SAKAI DAISUKE ET AL: "Successful fishing for nucleus pulposus progenitor cells of the intervertebral disc across species", JOR SPINE, vol. 1, no. 2, 1 June 2018 (2018-06-01), pages e1018, XP093000278, ISSN: 2572-1143, Retrieved from the Internet <URL:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6686801/pdf/JSP2-1-e1018.pdf> DOI: 10.1002/jsp2.1018 * |
See also references of WO2020196615A1 * |
TEKARI ADEL ET AL: "Angiopoietin-1 receptor Tie2 distinguishes multipotent differentiation capability in bovine coccygeal nucleus pulposus cells", STEM CELL RESEARCH & THERAPY, vol. 7, no. 1, 23 May 2016 (2016-05-23), XP055796514, Retrieved from the Internet <URL:http://link.springer.com/content/pdf/10.1186/s13287-016-0337-9.pdf> DOI: 10.1186/s13287-016-0337-9 * |
YAN ZHENGJIAN ET AL: "A Novel Organ Culture Model of Mouse Intervertebral Disc Tissues", CELLS TISSUES ORGANS, vol. 201, no. 1, 9 October 2015 (2015-10-09), CH, pages 38 - 50, XP093000272, ISSN: 1422-6405, Retrieved from the Internet <URL:https://www.karger.com/Article/Pdf/439268> DOI: 10.1159/000439268 * |
Also Published As
Publication number | Publication date |
---|---|
CN113874489A (zh) | 2021-12-31 |
JPWO2020196615A1 (de) | 2020-10-01 |
AU2020244911A2 (en) | 2021-12-09 |
BR112021019037A2 (pt) | 2021-11-30 |
CA3134256A1 (en) | 2020-10-01 |
KR20210153066A (ko) | 2021-12-16 |
EP3950934A1 (de) | 2022-02-09 |
SG11202110461UA (en) | 2021-10-28 |
JP6712740B1 (ja) | 2020-06-24 |
WO2020196615A1 (ja) | 2020-10-01 |
AU2020244911A1 (en) | 2021-11-11 |
US20220056418A1 (en) | 2022-02-24 |
IL286558A (en) | 2021-12-01 |
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