EP3641943A1 - Zentrifuge - Google Patents
ZentrifugeInfo
- Publication number
- EP3641943A1 EP3641943A1 EP18733249.9A EP18733249A EP3641943A1 EP 3641943 A1 EP3641943 A1 EP 3641943A1 EP 18733249 A EP18733249 A EP 18733249A EP 3641943 A1 EP3641943 A1 EP 3641943A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- rotor
- reaction vessel
- centrifuge
- housing
- vessel unit
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000006243 chemical reaction Methods 0.000 claims abstract description 119
- 238000004140 cleaning Methods 0.000 claims abstract description 21
- 238000006073 displacement reaction Methods 0.000 claims description 20
- 238000001514 detection method Methods 0.000 claims description 15
- 230000008878 coupling Effects 0.000 claims description 10
- 238000010168 coupling process Methods 0.000 claims description 10
- 238000005859 coupling reaction Methods 0.000 claims description 10
- 238000009423 ventilation Methods 0.000 claims description 10
- 230000003287 optical effect Effects 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 7
- 229920001343 polytetrafluoroethylene Polymers 0.000 claims description 5
- 239000004810 polytetrafluoroethylene Substances 0.000 claims description 5
- 238000011156 evaluation Methods 0.000 claims description 2
- 230000005484 gravity Effects 0.000 claims description 2
- 230000002209 hydrophobic effect Effects 0.000 claims description 2
- 239000007788 liquid Substances 0.000 abstract description 40
- 239000004033 plastic Substances 0.000 description 5
- 239000000463 material Substances 0.000 description 3
- 238000007789 sealing Methods 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 239000006260 foam Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 238000005507 spraying Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241000237983 Trochidae Species 0.000 description 1
- 230000001464 adherent effect Effects 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- UQMRAFJOBWOFNS-UHFFFAOYSA-N butyl 2-(2,4-dichlorophenoxy)acetate Chemical compound CCCCOC(=O)COC1=CC=C(Cl)C=C1Cl UQMRAFJOBWOFNS-UHFFFAOYSA-N 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000012459 cleaning agent Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000005202 decontamination Methods 0.000 description 1
- 230000003588 decontaminative effect Effects 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000005187 foaming Methods 0.000 description 1
- 239000003779 heat-resistant material Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- -1 polytetrafluoroethylene Polymers 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 238000009987 spinning Methods 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
- B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
- B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L13/00—Cleaning or rinsing apparatus
- B01L13/02—Cleaning or rinsing apparatus for receptacle or instruments
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B15/00—Other accessories for centrifuges
- B04B15/06—Other accessories for centrifuges for cleaning bowls, filters, sieves, inserts, or the like
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B7/00—Elements of centrifuges
- B04B7/02—Casings; Lids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B7/00—Elements of centrifuges
- B04B7/02—Casings; Lids
- B04B7/04—Casings facilitating discharge
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0829—Multi-well plates; Microtitration plates
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L9/00—Supporting devices; Holding devices
- B01L9/52—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips
- B01L9/523—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips for multisample carriers, e.g. used for microtitration plates
Definitions
- the present invention relates to a centrifuge for cleaning a reaction vessel unit having a rotor and a rotor space, in which the rotor is arranged and rotatably mounted, wherein the rotor has a receiving area for receiving the reaction vessel unit.
- EP 937502 A2 describes a method for handling a microtiter plate, wherein the microtiter plate is cleaned by centrifugation.
- the microtiter plate is placed over a conveyor belt in the rotary housing, so that the openings of the microtiter plate are directed away from the axis of rotation.
- This foam can quickly fill much of the volume of the rotor and exit at the door. Also, the foaming material can not be pumped out well by the pump described, but instead remains in the rotor chamber or in the drainage channel.
- the close distance of the rotor from the trough is mainly due to the cylindrical shape of the rotor space, which is chosen to produce the desired circulation wind. It is known that arise in cylindrical rotor chambers of centrifuge through the rotating rotor circulation winds. In this context, reference is made to US 2007/0037684 A1, DE 103 55 179 A1, DBP 1033446, EP 2 705 903 A1 and DE 2404036.
- DE 10 2008 042 971 A1 discloses a centrifuge in which a magnetic device is integrated in order to thereby hold magnetizable particles within a reaction vessel by means of the magnetic force.
- CN 102175855 A discloses a fully automatic 360 ° plate washing machine. The axis of rotation of this machine runs parallel to the horizontal plane, allowing the washing of multiple plates simultaneously in one housing, which can increase efficiency and greatly reduce costs.
- US 4,953,575 relates to a washing device for cuvettes. For this, the cuvettes are placed in a holder in a rotor. Turning the rotor removes the liquid from the cuvettes.
- the disclosed centrifuge housing has at its lowest point an opening through which the removed liquid can leave the housing.
- JP 2009264927 A discloses a device comprising a drum in which a microplate can be placed.
- the drum can be loaded with several microtiter plates, which then rotate around a horizontal axis of rotation.
- the drum is loaded with the microtiter plate so that its openings are directed towards the interior of the drum.
- the invention has for its object to provide a centrifuge for cleaning a reaction vessel unit having a rotor and a rotor chamber in which the rotor is rotatably mounted, so that the structure of the centrifuge is simpler than in the conventional centrifuges described above and a reliable operation is possible.
- a further object of the present invention is to develop a centrifuge described at the outset for cleaning a reaction vessel unit in such a way that the risk of contamination of the reaction vessel units is reduced.
- the present invention has for its object to provide a centrifuge for cleaning a reaction vessel unit, with a fast, thorough and reliable cleaning should be possible.
- a centrifuge for cleaning a reaction vessel unit having a rotor and a rotor space, in which the rotor is arranged and rotatably mounted.
- the rotor has a receiving area for receiving the reaction vessel unit.
- the rotor space is limited by a housing.
- the centrifuge is characterized in that the housing below the rotor has a gutter and the inner surfaces of the housing adjacent to the channel form a funnel, which opens into the channel.
- the funnel forming inner surfaces of the housing have a curvature which is substantially greater than the curvature of a worn by the rotor in one revolution with its outer edges cylindrical surface.
- the radius of curvature of these inner surfaces forming the funnel is preferably at least 0.5 m, in particular at least 1 m, and is preferably infinite, that is, the inner surfaces forming the funnel are planar.
- the funnel-forming inner surfaces hereinafter referred to as funnel surfaces, preferably extend laterally from the gutter to the outer edge of a rotational volume of the rotor, that is, the funnel and the gutter in plan view cover the complete vertical projection of the rotor. In other words, this means that the rotor in any position, even in a horizontal position, does not extend beyond the lateral edge of the funnel.
- Such a formation of the funnel surfaces with a small curvature has the effect that one revolution of the rotor comes closest to a central region of the funnel surface and a greater distance from both the outer edge and the central edge, which is formed adjacent to the groove has. In these areas with a greater distance from the rotor there is a small air flow when turning the rotor, so that the liquids collect here and flow away along the funnel surface.
- the distance of the channel from a rotation axis about which the rotor rotates is preferably at least 1.31 times the maximum radius of the rotor and in particular at least 1.2 times or 1.3 times the maximum radius of the rotor rotor. This distance is measured from the top of the trough to the axis of rotation.
- a free space is created in the funnel, in which liquid can temporarily accumulate. If a reaction vessel unit with large-volume reaction vessels is cleaned, which are almost completely filled, then at a first or in the first rotations of the rotor a large part of the liquid contained in the reaction vessels emptied at once. This can accumulate in this space without this liquid comes into contact with the rotating rotor. The liquid can then gradually drain from this space over the gutter.
- the inner surface of the housing is preferably coated with a smooth layer at least in the area of the funnel and the gutter.
- This layer may be a hydrophobic layer which is advantageous for draining off an aqueous solution.
- This layer can be formed, for example, from PTFE (polytetrafluoroethylene).
- the axis of rotation of the rotor is preferably arranged parallel to a standing surface of the housing. As a result, the axis of rotation of the rotor is arranged horizontally during operation.
- Such an arrangement of the axis of rotation allows a simple loading of the centrifuge with a reaction vessel unit, since this can be introduced with the openings of the reaction vessels facing up when inserted into the rotor chamber.
- reaction vessel units with large volume reaction vessels e.g., 96 reaction well microtiter plate
- the liquid does not necessarily adhere completely due to capillary forces in reaction vessels.
- such reaction vessel units can be turned by 180.degree.
- the channel preferably has an inclination with respect to the footprint of the housing. At the lower end of the channel, this opens into an outlet opening of the housing. At this outlet opening a hose may be connected, with which the liquid is passed into a container.
- This arrangement should be designed so that no back pressure is generated when the liquid is discharged. This can be done for example by providing a ventilation opening in the container.
- the housing has a vent opening opening into the rotor chamber. If the rotor space is otherwise closed in a substantially airtight manner, when the liquid drains off, when it completely fills the cross section of the outlet opening, a negative pressure in the rotor space could occur arise, which would counteract the further flow of the liquid. This is prevented by the provision of a ventilation opening.
- a filter such as an activated carbon filter, is provided, which prevents germs from entering the rotor space from the outside.
- a ventilation opening is preferably provided in the region of an end wall of the housing, to which liquid is not sprayed directly from the reaction vessel unit during centrifuging.
- the ventilation opening may also be shielded with a screen relative to the rotor space, wherein the screen is arranged at a distance from the ventilation opening.
- Such a centrifuge has a housing with an opening for feeding or withdrawing a reaction vessel unit to and from the rotor space.
- This opening can be closed by means of a door, which is typically actuated automatically. If this door does not seal perfectly, it forms a ventilation opening even when closed.
- the door is provided with a filter element arranged at its closing edge, so that the air flowing in at the edges of the door must pass through the filter.
- a centrifuge for cleaning a reaction vessel unit, which has a rotor and a rotor space, in which the rotor is arranged and rotatably supported.
- the rotor has a receiving area for receiving a reaction vessel unit.
- the centrifuge is provided with a housing which limits the rotor space. Furthermore, the centrifuge has a drive for rotating the rotor.
- the centrifuge is designed with an exchange module which comprises the rotor and a housing section of the housing, this housing section enclosing the rotor.
- the replacement module is detachably formed from the remaining parts of the centrifuge.
- This replacement module preferably comprises only mechanical elements, such as rotor, housing, bearings, etc. and no electronic elements. This makes it possible to autoclave the replacement module and thus clean and sterilize it without residue.
- a centrifuge can be cleaned by introducing cleaning agent, which is filled, for example, into the reaction vessel of a reaction vessel unit and then centrifuged in the centrifuge, or introduced into the rotor space by means of appropriate nozzles. However, such cleaning is not always completely residue-free.
- An exchange module comprises a few mechanical parts, which cause only a small part of the manufacturing costs with respect to the entire centrifuge.
- a centrifuge has a drive for rotating the rotor, a control device for controlling the rotational movement of the centrifuge and further components, such as a dispensing device and / or a loading and unloading device.
- Such a loading and unloading device can be relatively complex, in particular if it is designed with a detection device for determining the position of a displacement bar of the loading and unloading device.
- This replacement module may therefore be a consumable that is used only once or a predetermined amount of time or a predetermined number of cleaning operations. If the replacement module is provided as a consumable, then it may also be expedient to provide parts thereof made of plastic, in particular as injection-molded articles, such as, for example, the rotor and / or the housing section of the replacement module. On the other hand, the replacement module can also be made heat-resistant, so that it can reliably withstand temperatures of up to 100 ° C. and preferably up to 150 ° C., for example, so that the replacement module can be cleaned by means of autoclaving. The fact that the replacement module is separable from the electronic components of the centrifuge, it is possible to form the replacement module heat resistant. This allows a residue-free cleaning and reuse of the replacement module.
- Such an exchange module preferably has a fastening mechanism with which it can be easily and quickly coupled to the remaining parts of the centrifuge, fixed and coupled to the other functional elements, such as drive device, pipetting device, camera and / or loading and unloading device in that the function of these elements is executable.
- the centrifuge according to one of the above-described embodiments may be provided with a loading and unloading device which provides a rigid displacement. Bestange for positioning a reaction vessel unit in or for removing a reaction vessel unit from the rotor.
- the displacement rod is displaceably arranged between an unloading position in which it extends in the rotor space through the rotor and a loading position in which it is pulled out at least from the region of the rotor space which is claimed by the rotor in one revolution. can be moved.
- a linear drive can be provided to move the sliding bar between the unloading position and the loading position.
- a coupling element At a free, located in the rotor chamber end of the displacement rod, a coupling element may be arranged.
- the coupling element is used for reconnecting the displacement rod with a reaction vessel unit or a carrier unit for a reaction vessel unit.
- the coupling element can have a latching element which can engage with a counter-latching element provided on the reaction-vessel unit or on the carrier unit, wherein at least the latching element or the counter-latching element is elastically mounted.
- the counter-detent element of the reaction vessel unit or the carrier unit can be elastically mounted and coupled to a locking bracket, so that the locking bracket is pivotable between two positions, wherein an unlocking position is taken when the latching element and the counter-latching element are locked together, and a locking position is taken, when the locking element and the counter-locking element are separated from each other, wherein the locking bracket has a locking element which can engage in a locking position with a corresponding counter-locking element.
- the sliding rod preferably has a smooth surface.
- the sliding rod may be hollow and open at the rear end facing away from the rotor space, and a threaded rod may be provided coaxially with the sliding rod.
- the threaded rod can be in meshing engagement with a thread connected to the sliding rod, so that a translational movement of the sliding rod is carried out by a rotational movement of the threaded rod.
- the threaded rod dips here in the rear end in the sliding bar.
- the displacement rod can be guided through an opening in a housing wall, wherein in the region of the opening a sealing element ment is provided, which seals the sliding bar against the housing. If an exchange module is provided, then an additional housing wall is preferably provided which is not part of the replacement module and in which the opening is formed with a sealing ring. In a corresponding opening of the housing wall of the replacement module, a further sealing element may be provided.
- a detection device For determining the position of the displacement rod, a detection device may be provided. This detection device is preferably an optical detection device.
- the centrifuge can have a pipetting or dispensing unit, wherein the dispenser unit preferably has a plurality of pipetting or dispensing nozzles.
- the dispenser nozzles are preferably arranged side by side along a line, this line extending transversely to the direction of movement of the reaction vessel unit during loading or unloading.
- the nozzles of the dispenser unit are preferably arranged adjacent to an opening for loading and unloading the centrifuge with the reaction vessel unit.
- an optical detection unit may be provided which is arranged adjacent to the rotor space in the movement space of the reaction vessel unit in order to scan it.
- this detection unit comprises a line camera for scanning a reaction vessel unit in a cell-like manner, one scanning line being oriented approximately perpendicular to the direction of movement of the reaction vessel unit.
- the detection unit may include a color camera to spectrally scan a reaction vessel unit.
- the optical detection unit can also be designed for 3D scanning.
- the centrifuge can have an evaluation device with which the signals obtained with the optical detection device are automatically evaluated according to one or more of the following parameters:
- a spraying device for spraying a decontamination solution or cleaning solution into the interior can be provided.
- the housing may be provided in the region of the rotor space with one or more windows.
- the centrifuge can have a control device which detects the position of a reaction vessel unit or a carrier of a reaction vessel unit and these via an interface to another device, such as a robot or another component in the centrifuge, such as a dispensing unit so that the further device or the further component can obtain the position of the reaction vessel unit or of the carrier of the reaction vessel unit.
- a control device which detects the position of a reaction vessel unit or a carrier of a reaction vessel unit and these via an interface to another device, such as a robot or another component in the centrifuge, such as a dispensing unit so that the further device or the further component can obtain the position of the reaction vessel unit or of the carrier of the reaction vessel unit.
- a method of cleaning a reaction vessel unit with a centrifuge having a rotor The reaction vessel unit is arranged in the rotor with the openings of the reaction vessel pointing radially outward.
- the rotor is rotated together with the reaction vessel unit about an axis of rotation, so that the contents of the reaction vessels is ejected. Alone by the rotational movement of the rotor and gravity, the contents of the reaction vessels in the gutter and is driven outwards therefrom. In this case, no suction pump is used to suck the liquid out of the rotor chamber.
- a method for cleaning a reaction vessel unit with a centrifuge having a rotor, wherein the reaction vessel unit is arranged in the rotor with the openings of the reaction vessel pointing radially outward.
- the rotor is rotated together with the reaction vessel unit about an axis of rotation, so that the contents of the reaction vessels is ejected.
- An exchange module comprising a housing section and the rotor, after cleaning one or more Reaction vessel units separated from the remainder of the centrifuge and either cleaned or replaced by another exchange module.
- a centrifuge can be used, as explained above.
- FIG. 2 shows the part of the housing from FIG. 1 in a sectional view looking diagonally from the front
- FIG. 3 shows the part of the housing from FIG. 1 in a longitudinal section
- FIG. 4 parts of a centrifuge with the housing part from FIG. 1 in a longitudinal section
- FIG. 5 schematically shows another embodiment of a centrifuge with an exchange module
- Figure 6 schematically another embodiment of a centrifuge with an exchange module in a longitudinal section.
- a centrifuge 1 according to the invention (FIG. 4) has a rotor 2, a housing 3, a drive device 4 for rotating the rotor 2 about an axis of rotation 5.
- the rotor has at least one receiving area 6 for receiving a reaction vessel unit 7.
- the reaction vessel unit 7 is usually a microtiter plate.
- Such microtiter plates may be formed with a different number of reaction vessels.
- the individual reaction vessels are so thin that a liquid normally adheres therein alone due to capillary forces, so that even when placing such a microtiter plate with its openings down, the liquid does not flows. For microtiter plates with fewer reaction vessels, which are larger, this is not true.
- Such a reaction vessel unit 7 can be inserted alone into a recording device 6 or on a carrier unit.
- a carrier unit is used which has a coupling element which can be coupled to a loading and unloading device 8.
- a loading and unloading device is described for example in DE 10 2016 101 163. It will be explained in more detail below.
- the housing 3 delimits a rotor chamber 9.
- the region of the housing 3 delimiting the rotor chamber 9 is formed from a lower shell 10, upper shell 11, front end wall 12 and rear end wall 13.
- In the front end wall 12 and rear end wall 13 are each a ball bearing 14, in which a continuous shaft 15 of the rotor 2 is rotatably mounted.
- the center line of the shaft 15 forms the axis of rotation 5.
- the axis of rotation 5 extends parallel to a base surface 16, which is formed by the underside of the lower shell 10.
- the rear end of the shaft 15 is coupled to the drive device 4.
- the further part of the housing which adjoins the back of the housing, contains the drive device 17, the loading and unloading device 8 and a central control device (not shown), with which all components of the centrifuge 1 are controlled.
- a balcony 18 is mounted on the outside, which serves to receive a reaction vessel unit 7.
- a loading and unloading opening 19 is formed in the front end wall 12, through which a reaction vessel unit 7 can be inserted into the rotor chamber 9 and pushed out again.
- the loading and unloading opening 19 is provided with a pivotable door 20, so that the rotor space can be closed.
- a dispenser unit with a plurality of dispenser nozzles and / or an optical detection unit, in particular in the form of a line scan camera can be provided.
- the loading and unloading device 8 has a displacement rod (not shown), which can be moved horizontally through the rotor chamber 9 through a passage opening 21 in the rear end wall 13 with its free end.
- the loading and unloading device 8 has a linear drive, so that the displacement bar can be moved linearly along its longitudinal direction.
- the displacement rod has at its free end a coupling element which can be coupled to a corresponding coupling element on the carrier unit or on a reaction vessel unit 7, so that the carrier unit with a reaction vessel unit or the reaction vessel unit directly by moving the displacement rod from the balcony 18 through the Be and the discharge opening 19 can be moved into the rotor space 9, wherein the rotor 2 is arranged here with a receiving area 6 adjacent to the loading and unloading opening 19, so that the carrier unit or the reaction vessel unit is moved into the receiving area 6 of the rotor 2.
- the coupling between the displacement rod and the support unit or the reaction vessel unit 7 can be released, so that the support unit or the reaction vessel unit is freely movable in the rotor 2 and the rotor can be rotated accordingly with this unit.
- the carrier unit or reaction vessel unit 7 can be pushed back out of the receiving area 6 of the rotor 2 through the loading and unloading opening 19 onto the balcony 18.
- the reaction vessel unit 7 can be removed, for example by means of a robot.
- the lower shell 10 has a groove 22, which runs approximately parallel to the axis of rotation 5.
- the channel 22 extends from the rear end wall 13 into the region to the front end wall 12, wherein it is inclined or sloping toward the front ( Figure 4).
- an outlet opening 23 is formed, at which the channel 22 opens.
- a connection pin 24 is arranged, to which a hose 25 can be connected.
- the tube 25 usually opens into a receptacle (not shown), in which the liquids are received, which are ejected in the centrifuge 1 from the reaction vessels of the reaction vessel unit 7.
- the container preferably has a ventilation opening or the tube extends through the container with some play, so that liquid emerging from the centrifuge through the tube 25 does not generate a back pressure in the container.
- the lower shell 10 has, adjacent to the gutter 22, inner surfaces which extend obliquely rising outwards from an upper edge of the gutter 22 (FIG. 2). These inner surfaces thus form a funnel 26 and are referred to below as funnel surfaces 27.
- the funnel surfaces 27 are inclined at an angle of about 30 ° to 60 ° relative to the horizontal.
- Substantially planar means that the funnel surfaces have a radius of curvature of more than 0.5 m, and preferably more than 1 m.
- the funnel surfaces 27 extend laterally beyond the region of the rotor 2, even if it is in its horizontal position.
- the inner surfaces of the lower shell 10 extend approximately vertically upward. They thus form vertical surfaces 28.
- the upper shell 1 1 is fixed, which has a trough-shaped form of a semi-circular cross-sectional shape.
- the inner surface of the upper shell 1 1 goes flush on the vertical surface 28.
- the cross section of the housing 3 is therefore not, as known from the prior art, cylindrical, but has only in the upper region of the shell 1 1 has a cylindrical curvature, whereas the lower shell 10 tapers in cross section in a funnel shape and ends in the groove 22.
- the channel 22 is slightly offset from the funnel-shaped lower shell 10 down and has two approximately vertically disposed side walls 37 a, 37 b.
- the channel itself is formed with an inclination, so that a liquid therein is drained.
- the lower shell 10 and the upper shell 1 1 is formed of metal.
- the inner surfaces of the lower shell 10 and the upper shell 1 1 are coated with a smooth plastic layer, so that liquids that are ejected from the reaction vessels of the reaction vessel units 7, run quickly along the inner surfaces, are guided by the hopper 26 to the channel 22 and out there emerge from the rotor space 9.
- the plastic layer is made of PTFE.
- the upper edge of the channel 22 is spaced from the axis of rotation 5 at least 1.32 times the maximum radius of the rotor 2. As a result, a clearance is formed in the hopper 26, which is not touched by the rotor 2 in one revolution. In This space can accumulate fluid.
- a maximum level 29 of the liquid is shown, which can accumulate in the hopper 26, without coming into contact with the rotor. This makes it possible, in the case of large-volume reaction vessels of a reaction vessel unit 7, to empty the main part of the liquid contained therein at once, to collect it in the funnel 26, so that it can flow off gradually through the outlet opening 23.
- the groove is bounded by approximately vertical side walls 37a, 37b, even if an air flow in the direction of rotation 38 is generated, it can no longer drive the liquid out of the groove. Once inside the channel 22 located liquid is thus trapped therein and can escape only through the outlet opening 23. In the exemplary embodiment shown in FIG. 2, an air flow can strike against the side wall 37a, which is arranged downstream in the groove 22 in the direction of rotation 38 of the rotor. But since the side wall 37a is approximately perpendicular to the flow direction, the liquid in the gutter can not be driven back into the rotor space. In principle, a channel with an approximately vertical side wall on the side of the channel 22 following in the direction of rotation 38 is sufficient. However, in terms of production, it is expedient to produce a channel with two approximately vertical side walls 37a, 37b.
- the centrifuge 1 in turn has a housing 3 with a rotor 2, a drive device 4, a balcony 18, a loading and unloading device 8 and a central control device 30.
- a rotor chamber 17 is closed at the top by a pivotable lid 31.
- the pivotable cover 31 is connected with a pivot joint with the remaining part of the housing 3 in the region of the rear end wall 13.
- the rotor 2 is mounted, which is part of a replacement module 32 in this embodiment.
- the replacement module 32 has an exchange module housing 33, which substantially completely surrounds the rotor 2 and fits into the rotor chamber 17 of the housing 3 with little play.
- the exchange module housing 33 has a front end wall 12a, a rear end wall 13a, a lower shell 10a and an upper shell 11a.
- These walls 12a, 13a, or shells 10a, 11a are thin-walled walls, which preferably form inner surfaces which correspond to the shape of the inner surfaces of the rotor space 9 of the above-explained first embodiment.
- the replacement module 33 can be removed from the housing 3 in the vertical direction 34 and replaced by a further replacement module 33 or cleaned and then reused.
- the loading and unloading device 8 and the drive means 4 are moved horizontally a distance away from the rotor chamber 17.
- the free end of the sliding bar of the loading and unloading device 8 and a shaft journal of the drive device 4 is pulled out of the rotor chamber 17, so that they are not a movement of the exchange module housing 33 up in the way.
- the loading and unloading device 8 and the drive means 4 are pushed back horizontally in the direction of the arrow 34.
- the replacement module housing 33 in turn has a channel 22, which opens to an outlet opening 23 of the housing 3.
- FIG. 6 shows a variant of the second exemplary embodiment, in which instead of a pivotable cover 31 for limiting the rotor chamber 17, a detachable bare hood 35, which has the front end wall 12 and a, the rotor chamber 17 upwardly delimiting top wall 36.
- This hood 35 can be pulled off in the horizontal direction (arrow 34), so that the replacement module housing 33 is exposed.
- the replacement module housing 33 can be withdrawn in the horizontal direction (arrow 34) from the remaining part of the housing 3.
- no mechanism for moving the drive device 4 or the loading and unloading device 8 is provided.
- the replacement module housing 33 can be replaced or removed for cleaning and replaced.
- the replacement module housing 33 is to be cleaned, for example by autoclaving, then it is expedient that it is made of heat-resistant materials, such as metal. However, it may also be expedient to completely replace the replacement module after a certain use. On the one hand, the reaction vessel units can be cleaned of substances which attack the exchange module 32 and, for example, corrode. Then it makes sense to completely replace the replacement module 32 after a predetermined period of operation of a few weeks to a few months. If such replacement modules 32 are used as consumables, then it may also be expedient to form them essentially from plastic.
- the replacement module housing 33 is preferably formed of a material with high chemical resistance, such as PTFE.
- the rotor is preferably made of a hard plastic material, in particular an injection molded part.
- Both the hood 35 and the pivotable lid 31 can be attached to the rest of the housing 3 with a latch or snap mechanism.
- Reaction vessel unit 25 hose
- Rotor chamber 28 vertical surface
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- Health & Medical Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Centrifugal Separators (AREA)
Abstract
Description
Claims
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
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DE102017113583.9A DE102017113583A1 (de) | 2017-06-20 | 2017-06-20 | Zentrifuge |
PCT/EP2018/066495 WO2018234420A1 (de) | 2017-06-20 | 2018-06-20 | Zentrifuge |
Publications (1)
Publication Number | Publication Date |
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EP3641943A1 true EP3641943A1 (de) | 2020-04-29 |
Family
ID=62705600
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EP18733249.9A Pending EP3641943A1 (de) | 2017-06-20 | 2018-06-20 | Zentrifuge |
Country Status (6)
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US (1) | US11738354B2 (de) |
EP (1) | EP3641943A1 (de) |
CN (1) | CN110913992A (de) |
CA (1) | CA3067655A1 (de) |
DE (1) | DE102017113583A1 (de) |
WO (1) | WO2018234420A1 (de) |
Families Citing this family (12)
Publication number | Priority date | Publication date | Assignee | Title |
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DE102017113583A1 (de) * | 2017-06-20 | 2018-12-20 | Bluecatbio Gmbh | Zentrifuge |
EP4347137A1 (de) * | 2021-05-31 | 2024-04-10 | Cytena Gmbh | Zentrifuge zum rotieren eines probenträgers |
WO2023007224A1 (en) * | 2021-07-29 | 2023-02-02 | Ecole Polytechnique Federale De Lausanne (Epfl) | Biological material pooling device for biological or biomolecule purification |
DE102021121265B4 (de) | 2021-08-16 | 2024-03-07 | Bluecatbio Gmbh | Dispensiervorrichtung, Zentrifuge mit einer solchen Dispensiervorrichtung und Verfahren zum Reinigen von Dispensierdüsen |
US20230072202A1 (en) * | 2021-09-07 | 2023-03-09 | Grenova, Inc. | Laboratory well plate washing device and associated method |
DE102021124023A1 (de) | 2021-09-16 | 2023-03-16 | Bluecatbio Gmbh | Zentrifuge und Verfahren zum Reinigen einer Zentrifuge |
DE102021128326A1 (de) | 2021-10-29 | 2023-05-04 | Heinkel Holding Gmbh | Zentrifuge |
DE102021006144A1 (de) | 2021-12-13 | 2023-06-15 | BlueCat Solutions GmbH | Reaktionsgefäßeinheit, Verfahren zum selektiven Entfernen einer Flüssigkeit sowie zum Einbringen einer einen Zielstoff enthaltenden Flüssigkeit aus einem bzw. in ein Reaktionsgefäß einer Reaktionsgefäßeinheit |
DE102022102705A1 (de) | 2022-02-04 | 2023-08-10 | BlueCat Solutions GmbH | Auffangvorrichtung für eine Reaktionsgefäßeinheit und Reaktionsgefäßeinheit |
DE102022102701A1 (de) | 2022-02-04 | 2023-08-10 | Bluecatbio Gmbh | Ableitvorrichtung für eine Reaktionsgefäßeinheit, Zentrifuge und Verfahren zum Reinigen einer Reaktionsgefäßeinheit |
LU502092B1 (de) * | 2022-05-14 | 2023-11-14 | Cytena Gmbh | Zentrifuge zum Rotieren eines Probenträgers |
CN116422482B (zh) * | 2023-04-17 | 2023-10-27 | 河北冠能石油机械制造有限公司 | 一种离心机清洗装置 |
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DE1033446B (de) | 1955-05-13 | 1958-07-03 | Martin Christ Fa | Gekuehlter Rotorraum fuer hochtourige Laborzentrifuge |
DE2404036A1 (de) | 1974-01-29 | 1975-07-31 | Heraeus Christ Gmbh | Zentrifuge zur behandlung biologischer fluessigkeiten wie blut |
JPS57937Y2 (de) * | 1978-03-13 | 1982-01-07 | ||
FI884502A (fi) | 1988-09-30 | 1990-03-31 | Labsystems Oy | Tvaettningsanordning. |
JPH0427457A (ja) * | 1990-05-23 | 1992-01-30 | Matsushita Electric Ind Co Ltd | 遠心分離機および自動遠心分離装置 |
FI982645A (fi) | 1997-12-15 | 1999-06-16 | Wallac Oy | Menetelmä ja laite näytelevyjen käsittelemiseksi |
US6296602B1 (en) * | 1999-03-17 | 2001-10-02 | Transfusion Technologies Corporation | Method for collecting platelets and other blood components from whole blood |
EP1270078B1 (de) * | 2001-06-22 | 2004-09-15 | Jouan Italia S.R.L. | Vorrichtung und Verfahren zum automatischen Be- und Entladen von Zentrifugenbechern |
DE10135317A1 (de) | 2001-07-19 | 2003-01-30 | Bayer Ag | Umlenkring für eine selbstaustragende Zentrifuge |
DE10355179B4 (de) | 2003-11-26 | 2007-07-12 | Thermo Electron Led Gmbh | Luftgekühlte Zentrifuge |
US7422554B2 (en) | 2005-08-10 | 2008-09-09 | The Drucker Company, Inc. | Centrifuge with aerodynamic rotor and bucket design |
JP2009264927A (ja) | 2008-04-25 | 2009-11-12 | Micronics Kk | マイクロプレート処理装置 |
DE102008042971A1 (de) | 2008-10-20 | 2010-04-22 | Agilent Technologies Inc., Santa Clara | Zentrifuge mit Magneteinrichtung |
CN102175855A (zh) | 2011-01-13 | 2011-09-07 | 邱重任 | 一种全自动360度甩干的卧式酶标洗板机 |
GB201207178D0 (en) * | 2012-04-24 | 2012-06-06 | Bowyer Andrew | Miniaturised centrifiguration apparatus |
EP2705903A1 (de) | 2012-09-06 | 2014-03-12 | Eppendorf AG | Rotoreinrichtung, Zentrifugenkessel und Zentrifuge, und deren Herstellungsverfahren |
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WO2016052265A1 (ja) * | 2014-09-30 | 2016-04-07 | 日立工機株式会社 | 遠心機及び遠心機用スイングロータ |
DE102015108741A1 (de) | 2015-06-02 | 2016-12-08 | Gea Mechanical Equipment Gmbh | Separator |
DE102016101163A1 (de) * | 2016-01-22 | 2017-07-27 | Bluecatbio Gmbh | Zentrifuge |
DE102017113583A1 (de) * | 2017-06-20 | 2018-12-20 | Bluecatbio Gmbh | Zentrifuge |
DE102021124023A1 (de) * | 2021-09-16 | 2023-03-16 | Bluecatbio Gmbh | Zentrifuge und Verfahren zum Reinigen einer Zentrifuge |
-
2017
- 2017-06-20 DE DE102017113583.9A patent/DE102017113583A1/de active Pending
-
2018
- 2018-06-20 WO PCT/EP2018/066495 patent/WO2018234420A1/de unknown
- 2018-06-20 EP EP18733249.9A patent/EP3641943A1/de active Pending
- 2018-06-20 US US16/624,749 patent/US11738354B2/en active Active
- 2018-06-20 CA CA3067655A patent/CA3067655A1/en active Pending
- 2018-06-20 CN CN201880041332.0A patent/CN110913992A/zh active Pending
Also Published As
Publication number | Publication date |
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WO2018234420A1 (de) | 2018-12-27 |
US11738354B2 (en) | 2023-08-29 |
CA3067655A1 (en) | 2018-12-27 |
CN110913992A (zh) | 2020-03-24 |
US20210138485A1 (en) | 2021-05-13 |
DE102017113583A1 (de) | 2018-12-20 |
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