EP3337977A1 - Pompe péristaltique de délivrance d'échantillons continue - Google Patents

Pompe péristaltique de délivrance d'échantillons continue

Info

Publication number
EP3337977A1
EP3337977A1 EP16839908.7A EP16839908A EP3337977A1 EP 3337977 A1 EP3337977 A1 EP 3337977A1 EP 16839908 A EP16839908 A EP 16839908A EP 3337977 A1 EP3337977 A1 EP 3337977A1
Authority
EP
European Patent Office
Prior art keywords
disk
tubing
roller
periphery
rollers
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP16839908.7A
Other languages
German (de)
English (en)
Other versions
EP3337977B1 (fr
EP3337977A4 (fr
Inventor
Nathan Michael Gaskill-Fox
Daniel Nelson FOX
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bio Rad Laboratories Inc
Original Assignee
Bio Rad Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bio Rad Laboratories Inc filed Critical Bio Rad Laboratories Inc
Priority claimed from PCT/US2016/047877 external-priority patent/WO2017035020A1/fr
Publication of EP3337977A1 publication Critical patent/EP3337977A1/fr
Publication of EP3337977A4 publication Critical patent/EP3337977A4/fr
Application granted granted Critical
Publication of EP3337977B1 publication Critical patent/EP3337977B1/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
    • F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
    • F04B43/00—Machines, pumps, or pumping installations having flexible working members
    • F04B43/12—Machines, pumps, or pumping installations having flexible working members having peristaltic action
    • F04B43/1253—Machines, pumps, or pumping installations having flexible working members having peristaltic action by using two or more rollers as squeezing elements, the rollers moving on an arc of a circle during squeezing
    • F04B43/1261—Machines, pumps, or pumping installations having flexible working members having peristaltic action by using two or more rollers as squeezing elements, the rollers moving on an arc of a circle during squeezing the rollers being placed at the outside of the tubular flexible member
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
    • F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
    • F04B43/00—Machines, pumps, or pumping installations having flexible working members
    • F04B43/0009—Special features
    • F04B43/0054—Special features particularities of the flexible members
    • F04B43/0072—Special features particularities of the flexible members of tubular flexible members
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
    • F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
    • F04B43/00—Machines, pumps, or pumping installations having flexible working members
    • F04B43/12—Machines, pumps, or pumping installations having flexible working members having peristaltic action
    • F04B43/1215—Machines, pumps, or pumping installations having flexible working members having peristaltic action having no backing plate (deforming of the tube only by rollers)
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
    • F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
    • F04B43/00—Machines, pumps, or pumping installations having flexible working members
    • F04B43/12—Machines, pumps, or pumping installations having flexible working members having peristaltic action
    • F04B43/1253—Machines, pumps, or pumping installations having flexible working members having peristaltic action by using two or more rollers as squeezing elements, the rollers moving on an arc of a circle during squeezing
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
    • F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
    • F04B43/00—Machines, pumps, or pumping installations having flexible working members
    • F04B43/12—Machines, pumps, or pumping installations having flexible working members having peristaltic action
    • F04B43/1253—Machines, pumps, or pumping installations having flexible working members having peristaltic action by using two or more rollers as squeezing elements, the rollers moving on an arc of a circle during squeezing
    • F04B43/1276—Means for pushing the rollers against the tubular flexible member

Definitions

  • CONTINUOUS SAMPLE DELIVERY PERISTALTIC PUMP and U.S. Patent Application No. 15/242, 122, filed on August 19, 2016 and titled: “CONTINUOUS SAMPLE DELIVERY PERISTALTIC PUMP,” which are hereby incorporated by reference herein in their entirety.
  • Peristaltic pumps are positive displacement pumps. The fluid being pumped only contacts the flexible tubing and is not exposed to other pump components which could possibly cause cross-contamination. Both highly sterile fluids, as well as chemicals, can be pumped through the peristaltic pump, since the fluids only contact the flexible tubing. Peristaltic pumps are especially suited for pumping abrasives, viscous fluids and biological fluids.
  • a method of pumping a fluid through tubing that is positioned partially around the periphery of a first disk of a peristaltic pump and partially around the periphery of a second disk of the peristaltic pump may be provided.
  • the method may include orbiting a plurality of first rollers at a constant angular speed around the periphery of the first disk such that the first rollers are constantly pressed into contact with the periphery of the first disk, the tubing, or the periphery of the first disk and the tubing.
  • the first disk may include a first angular sector that is configured to cause the first rollers to move along a first section of the periphery of the first disk at a first tangential speed and a second angular sector that is configured to cause the first rollers to move along a second section of the periphery of the first disk at a second tangential speed less than the first tangential speed.
  • the method may also include orbiting a plurality of second rollers at the constant angular speed around the periphery of a second disk such that the second rollers are constantly pressed into contact with the periphery of the second disk, the tubing, or the periphery of the second disk and the tubing.
  • the second disk may be configured to cause each second roller to move at substantially the second tangential speed.
  • the method may also include increasing the pressure of a portion of the fluid in the tubing between one first roller and one second roller by causing the one first roller to fully compress the tubing in the first angular sector and simultaneously causing the one second roller to fully compress the tubing in a first section of the periphery of the second disk and moving, after increasing the pressure of the portion of the fluid, the portion of the fluid through the tubing at a constant pressure towards an output of the tubing by causing the one first roller to fully compress the tubing in the second angular sector and simultaneously causing the one second roller to fully compress the tubing.
  • the first disk may have a first nominal radius throughout at least part of the first angular sector and a second nominal radius throughout the second angular sector, the second disk may have the second nominal radius, and the first nominal radius may be larger than the second nominal radius.
  • the first disk may gradually transition in radius from the first radius to the second radius in between the first angular sector and the second angular sector.
  • moving the portion of the fluid through the tubing at a constant pressure towards an output of the tube may further include causing, after the one first roller has moved along the second section of the periphery of the first disk, the one first roller to move along a third angular sector of the first disk that includes a third section of the periphery of the first disk , the one first roller to fully compress the tubing at at least the beginning of the third section, and the one first roller to not fully compress the tubing at at least the end of the third section of the periphery of the first disk.
  • Moving the portion of the fluid through the tubing at the constant pressure towards an output of the tube may also include causing another second roller to fully compress the tubing against the second disk before causing the one first roller to not fully compress the tubing at at least the end of the third section of the periphery of the first disk.
  • the first disk may be configured to cause the one first roller to move along the third section at the second tangential speed.
  • the method may further include causing another first roller to fully compress the tubing before causing the one first roller to not fully compress the tubing at at least the end of the third section of the periphery of the first disk.
  • the method may further include causing the one second roller to fully compress the tubing when the one first roller is at least at the beginning of the third section of the periphery of the first disk and not to compress the tube when the one first roller is at least at the end of the third section of the periphery of the first disk.
  • the method may further include drawing fluid into the tubing through an inlet by causing one of the first rollers to fully compress the tube and orbit around at least part of the periphery of the first disk.
  • orbiting the plurality of first rollers at the constant angular speed around the periphery of the first disk and orbiting the plurality of second rollers at the constant angular speed around the periphery of the second disk may include fixing the first disk and the second disk in a position and causing the plurality of first rollers to orbit around the first disk and causing the plurality of second rollers to orbit around the second disk.
  • the output may be configured to supply the fluid to one of a flow cell or a cuvette.
  • the output of the tubing may have a pressure that substantially matches the constant pressure of the portion of the fluid.
  • the output may be configured to supply the fluid to a nozzle of a flow cytometer.
  • an apparatus may be provided.
  • the apparatus may include a first disk that includes a first recess in the periphery of the first disk, the first recess configured to receive a first portion of tubing for conveying fluid; a first angular sector that has a nominal first radius and includes a first section of the periphery of the first disk; and a second angular sector that has a nominal second radius and includes a second section of the periphery of the first disk.
  • the second radius may be smaller than the first radius
  • the first section of the periphery of the first disk may be longer than the second section of the periphery of the first disk.
  • the apparatus may also include a second disk that is substantially circular, has the nominal first radius, and includes a second recess in the periphery of the second disk, the second recess configured to receive a second portion of the tubing.
  • the apparatus may also include a plurality of first rollers that are configured to orbit around the periphery of the first disk at a constant angular speed and that are also configured to, when the first portion of the tubing is in the first recess, constantly press into contact with the periphery of the first disk, the tubing, or the periphery of the first disk and the tubing.
  • the apparatus may also include a plurality of second rollers that are configured to orbit around the periphery of the second disk at the constant angular speed and that are configured to, when the second portion of the tubing is in the second recess, constantly press into contact with the periphery of the second disk, the tubing, or the periphery of the second disk and the tubing.
  • the first disk may be configured such that each first roller moves in the first angular sector at a first tangential speed while fully compressing the tubing and such that each first roller moves in the second angular sector at a second tangential speed while fully compressing the tubing
  • the second disk may be configured such that each second roller moves around the periphery of the second disk at the second tangential speed.
  • the first disk, second disk, first rollers, and second rollers may be configured to cause one first roller to fully compress the tubing while moving in the first angular sector and to simultaneously cause one second roller to fully compress the tubing while moving in a first section of the periphery of the second disk, and the first disk, second disk, first rollers, and second rollers may be further configured to cause, after the one first roller has moved past the first angular sector, the one first roller to fully compress the tubing in the second angular sector and to simultaneously cause the one second roller to fully compress the tubing.
  • the first disk may gradually transition in radius from the first radius to the second radius in between the first angular sector and the second angular sector.
  • the first disk may be further configured to cause, after the one first roller has moved along the second section of the periphery of the first disk, the one first roller to move along a third angular sector of the first disk that includes a third section of the periphery of the first disk.
  • the first disk may be further configured to cause the one first roller to move along the third section at the second tangential speed, the one first roller to fully compress the tubing at at least the beginning of the third section, and the one first roller to not fully compress the tube at at least the end of the third section.
  • the second disk may be further configured to cause another second roller to fully compress the tubing against the second disk before the one first roller is caused to not fully compress the tubing at at least the end of the third section of the periphery of the first disk.
  • the apparatus may further include a first roller support on which the first rollers are mounted and a second roller support on which the second rollers are mounted.
  • the first roller support and the second roller support may be configured to rotate about a common center axis at the constant angular speed.
  • the apparatus may further include the tubing that is positioned partially around the periphery of the first disk in the first recess and that is positioned partially around the periphery of the second disk in the second recess.
  • the first recess in the sections of the periphery of the first disk where the first rollers fully compress the tubing, the first recess may have a first depth that is less than the nominal outer diameter of the tubing, causing the tubing to extend past the periphery of the first disk such that the first rollers fully compress the tubing, and in the sections of the periphery of the second disk where the second rollers fully compress the tubing, the second recess may have a second depth that is less than the nominal outer diameter of the tubing and that causes the tubing to extend past the periphery of the second disk such that the second rollers fully compress the tubing.
  • the first disk may include a first adjustment plate and the adjustment plate may be movable with respect to the remainder of the first disk such that locations along the periphery of the first disk where full compression of the tubing occurs between the first disk and a first roller are tunable.
  • the second disk includes a second adjustment plate and the adjustment plate may be movable with respect to the remainder of the second disk such that locations along the periphery of the second disk where full compression of the tubing occurs between the second disk and a second roller are tunable.
  • a method of reducing pressure variations of a fluid that is pumped through a peristaltic pump may be provided.
  • the method may include creating a supply of pressurized fluid in a first stage of the peristaltic pump using a first disk to pressurize the fluid by causing first rollers to move at different speeds around a periphery of the first disk, pumping the pressurized fluid in a second stage of the peristaltic pump using a second disk to move the pressurized fluid to an output at a substantially constant pressure by causing second rollers to move at substantially equal speeds around a periphery of the second disk.
  • the first rollers may pivot around the first disk at a substantially constant angular rotational speed.
  • the first disk may have different radii at different angular locations on the first disk, which causes the first rollers to traverse longer and shorter paths around the periphery of the first disk, which causes the first rollers to traverse the periphery of the first disk at different speeds.
  • the second rollers may pivot around the second disk at a substantially constant angular rotational speed, the second disk being substantially round so that the second rollers traverse around the periphery of the second disk at a substantially constant peripheral speed.
  • a peristaltic pump that produces an output flow of fluid at a substantially constant output pressure may be provided.
  • the peristaltic pump may include a first section of flexible tube and a first disk.
  • the first section of flexible tube may be disposed in a recess in the first disk and wrapped around a peripheral portion of the first disk such that the first section of flexible tube protrudes from the recess at first predetermined locations around the periphery of the first disk and does not protrude from the recess at second locations around the peripheral portion of the first disk.
  • the first disk may also have different radii that extend from a pivot point on the first disk to the peripheral portion at different angular locations on the first disk.
  • the pump may also include first rollers that are biased against the peripheral portion of the first disk and that compress the first section of flexible tube wrapped around the peripheral portion of the first disk at the first predetermined angular locations, the first rollers being mounted to rotate around the pivot point at a substantially constant angular rotational speed so that the first rollers traverse shorter and longer paths around the periphery of the first disk, which causes the first rollers to traverse the periphery of the first disk at different speeds and thereby causes the fluid to be pressurized to create a pressurized fluid that flows from the first disk.
  • the pump may also include a second section of flexible tube and a second disk having a round shape and a pivot point at a center of the round shape.
  • the second section of flexible tube may be disposed in a recess in the second disk and wrapped around a peripheral portion of the second disk such that the second section of flexible tube protrudes from the recess at first predetermined locations around the peripheral portion of the second disk and does not protrude from the recess at second predetermined locations around the periphery of the second disk.
  • the pump may also include second rollers that are biased against the peripheral portion of the second disk that compress the second section of flexible tube wrapped around the peripheral portion of the second disk at the first predetermined locations around the periphery of the second disk, and the second rollers may be mounted so as to rotate around the pivot point of the second disk at a substantially constant angular rotational speed so that the second rollers move at a substantially constant speed on the peripheral portion of the second disk and generate an output flow of the fluid that has a substantially constant output pressure.
  • the peristaltic pump may include first adjustment plates disposed on the first disk adjacent to the peripheral portions of the first disk that provide an adjustment of the first predetermined locations where the first and second sections of flexible tube protrudes from the recess.
  • the peristaltic pump may further include second adjustment plates disposed on the second disk adjacent to the peripheral portion of the second disk that provide an adjustment of the first predetermined locations around the peripheral portion of the second disk.
  • Figure 1 is a schematic diagram of the first stage of an embodiment of a peristaltic pump.
  • Figure 2 is a schematic top diagram of the second stage of an embodiment of a peristaltic pump.
  • Figures 3 A and 3B are schematic diagrams illustrating initial positions of rollers on a disk for both the first stage and the second stage of the peristaltic pump.
  • Figures 4A and 4B are schematic illustrations of a second location of rollers on the disks of an embodiment of the first stage and the second stage.
  • Figures 5 A and 5B are schematic illustrations of the third location of rollers on the disks of an embodiment of the first stage and the second stage.
  • Figure 6 is a schematic cross-sectional view of a roller and a disk with the opening in a flexible tubing being fully open.
  • Figure 7 is a schematic illustration of a roller and a disk illustrating the flexible tubing having an opening that is only partially open.
  • Figure 8 is a schematic illustration of a roller and a disk with the flexible tubing having an opening that is fully closed.
  • Figure 9 is a schematic cross-sectional view of a roller and a disk and an adjustment plate to adjust the spacing between the roller and the disk.
  • Figure 10 is a schematic perspective view of an embodiment of a peristaltic pump.
  • Figures 11 A and 1 IB are schematic illustrations of Figures 3A and 3B.
  • FIG 1 is a schematic illustration of the first stage 100 of an embodiment of a peristaltic pump.
  • the first stage 100 of the peristaltic pump includes a first disk 102 having an irregular shape.
  • disk is used to refer to both a round disk and a non-round cam, and such items may have a circular or partially circular shape, as can be seen in Figure 1.
  • An intake tubing 101 is connected to an auto loader 134 that has an uptake probe 132.
  • the word "tubing,” as used herein, may refer to discrete sections of tubing that are joined together, e.g., via couplers, or a single length of unbroken tubing; it may also refer to different portions of such structures.
  • the autoloader 134 moves the uptake probe 132 to the wells that are formed in the well plate 136 (or other media and containers) to obtain biological samples that are then pumped through the peristaltic pump.
  • Figure 1 is but one example of the application of a peristaltic pump; it may be used in other contexts as well aside from a flow cytometer.
  • the peristaltic pump disclosed herein has two stages, the first stage 100 that is illustrated in Figure 1, and a second stage 200 that is illustrated in Figure 2.
  • An advantage of peristaltic pumps is that the fluid that is being pumped through a peristaltic pump does not touch any of the pump parts except for the flexible tubing, such as intake tubing 101 and flexible tubing 104.
  • a conventional peristaltic pump the tubing is wrapped around a disk or cam and two or more spring-loaded rollers are orbited around the disk or cam so that the tubing is compressed against the disk or cam.
  • the rollers as they compress the tubing against the disk or cam during their orbit of the disk or cam, force or squeeze the fluid through the tubing. In this manner, the fluid that is being pumped through the peristaltic pump can avoid being contaminated.
  • a disadvantage, however, of conventional peristaltic pumps is that the output pressure of the liquid varies substantially, such that the output is a pulsed output that pulses with the rotational speed of the peristaltic pump. In many applications, a pulsed output with varying output pressure of the fluid is unacceptable.
  • pulsations of fluid flow adversely affect flow cytometry data because such pressure fluctuations can cause the sample volume to fluctuate within the flow cell where flow cytometry measurements occur, thereby making it more difficult to properly quantify the number of particles or cells in the sample.
  • Some peristaltic pumps attempt to reduce this pulsation by using three or more rollers to average out or smooth out the pulsations, but the additional rollers decrease the lifespan of the flexible tubing of the peristaltic pump thereby leading to increased maintenance costs and pump downtime. For example, the tubing will experience 50% more wear and tear with three rollers instead of two.
  • substantially constant output pressure is desirable in many flow cytometry
  • the pulsing of the output liquid from a conventional peristaltic pump may be acceptable in many instruments and other applications. However, it would be much more desirable to have a substantially constant pressure output that does not pulse in many other applications of a peristaltic pump, e.g., in flow cytometers.
  • samples in flow cytometry may be taken from small volume containers, such as a 5 milliliter tube or 96-well plate, it is more difficult and complex to use an air compression pump that utilizes a seal with such containers.
  • Syringe pumps may also be used for flow cytometry, but such pumps are slow, have functional problems, are difficult to clean out or de-clog, and are unable to effectively draw samples of varying media and/or varying volumes.
  • the embodiments disclosed herein relate to a two-stage peristaltic pump that provides a substantially constant output pressure of the liquid being pumped through the peristaltic pump.
  • the first stage 100 is used to increase the pressure of the liquid in the tubing above the inlet pressure and both the first stage 100 and the second stage 200 pump, e.g., move, the liquid through the tubing to an output of the tubing.
  • the pump is configured to provide a substantially constant pressure which matches or exceeds the back pressure in order to prevent the fluid from flowing backward, into, and through the pump.
  • first stage 100 includes the first disk 102, a plurality of first rollers 106, 108 (as discussed herein, the first disk and the second disk each have two rollers but additional rollers may be used; two rollers per disk results in the longest lifespan of the tubing by a significant margin, as discussed elsewhere herein), and flexible tubing 104 (a.k.a., "tubing" or "tubing 104").
  • the first rollers 106, 108 orbit around the periphery of the first disk 102 at a constant angular speed and during the orbit, each roller is caused to contact the periphery of the first disk 102, the tubing 104, or the periphery of the first disk 102 and the tubing 104, such that in various locations around the periphery of the first disk 102 one or more of the first rollers fully compresses the tubing 104.
  • such orbiting of rollers may also be referred to herein as the rollers rotating around or about the first disk; such orbiting also means the movement of rollers around, or encircling, the periphery of a disk.
  • Such orbiting or movement around the periphery of a disk is not intended to mean the rotation of each roller around each roller's individual pivot point, as discussed below, although the rollers may typically rotate about their own centers as they orbit the disk and roll along the periphery of the disk. Therefore, as each roller orbits around the periphery of the disk, each roller is also simultaneously rotating about its own pivot point.
  • the flexible tubing 104 is wrapped around the majority of the outside perimeter, i.e., the periphery, of the first disk 102.
  • the flexible tubing 104 is positioned in a recess, i.e., a trough, such that in certain positions around the periphery of the first disk 102, the tubing 104 extends past the periphery of the first disk 102 to enable the first rollers 106, 108 that orbit, i.e., rotate, around the periphery of the first disk 102 to compress the flexible tubing 104 in such locations; such compression of the tubing by the first rollers 106, 108, depends upon the depth of the trough or placement of the adjustment plate, as explained in more detail below.
  • roller support 128 is attached to roller brackets 112, 114 and causes the first rollers 106, 108 to rotate around the first disk 102 in a counterclockwise direction, as illustrated by arrow 111 in Figure 1.
  • the first rollers 106, 108 are forced against the periphery of the first disk 102 by springs 120, 122, respectively, so that the flexible tubing 104 is compressed against the first disk 102 in locations where the flexible tubing 104 is exposed to rollers 106, 108.
  • Roller brackets 112, 114 pivot around pivots 118, 116, respectively, that are mounted on the roller support 128.
  • First rollers 106, 108 rotate about points 140 and 142 as they roll along the outer periphery of the first disk 102 during their orbits of the first disk 102.
  • the axis of the rotation 110 of the roller support 128 is located so that radii 124, 125, 126, 127, 129 exist between the axis 110 and the periphery of the first disk 102.
  • the radii e.g., radius 124, 125 and 129
  • the first rollers 106, 108 move at a higher tangential speed along the periphery of the first disk 102 at radii 124, 125, 129 than at radii 126, 127 for a given angular velocity of the roller support 128.
  • FIG. 2 is a schematic top view of the second stage 200 of the peristaltic pump.
  • the second stage 200 includes a second disk 202 and second rollers 206, 208 and roller support 228 on which the second rollers 206, 208 are mounted; roller support 228 is configured to rotate in a clockwise direction around the second disk 202, as illustrated by arrow 211.
  • Roller support 228, in the illustrated embodiment rotates in the same direction and same angular speed as roller support 128; alternatively, the two roller supports may not be connected with one another, but may be driven at the same angular speed and in the same angular direction, e.g., by a common motor via two separate belt drives.
  • Figure 2 illustrates the second stage 200 from a top perspective.
  • the first stage 100 when viewed from a bottom perspective, moves in a counterclockwise direction, while the second stage, which is rotating in the same direction, is rotating in a clockwise direction when viewed from the top.
  • the rotation of the roller support 228 and the rotation of the roller support 128, of Figure 1 are synchronized and, in this embodiment, rotate at the same rotational, i.e. angular, speed and in the same direction.
  • rollers 206, 208 are mounted on roller brackets 214, 212, respectively, and are biased against the outer periphery of the second disk 202 by springs 222, 220, respectively.
  • rollers 206, 208 roll along the periphery of the second disk 202 and rotate around points 240 and 242, respectively, thereby squeezing or compressing the flexible hose 204 at locations along the periphery of the second disk 202 where the flexible hose 204 is exposed to the surface of the second rollers 206, 208.
  • the fluid can be pumped into any device for use and does not necessarily need to be pumped into a flow cytometer.
  • the fluid in the second stage 200 that is in front of each of the second rollers 206, 208 is not subjected to a pressure increase but is simply moved towards the output of the tubing at a constant pressure.
  • Back pressure of the system to which the fluid is being applied assists in maintaining a substantially constant pressure of the fluid pumped from the second stage.
  • the second rollers 206, 208 are biased against (i.e., constantly pressed into contact with) the periphery of the second disk 202, tubing 204, or the periphery of the second disk 202 and tubing 204 by springs 222, 220.
  • the roller brackets 212, 214 pivot around pivots 216, 218, respectively.
  • the second disk 202 has a substantially constant radius 232 so that the second rollers 206, 208 move at substantially the same tangential speed (there may be some minor variation in tangential speed of the rollers due, for example, to shifts in roller position due to the amount of tubing compression by the second rollers; generally speaking, the second rollers will be kept at the same nominal speed) around the periphery of the second disk 202.
  • the pressure of the pumped fluid e.g., the fluid that is pushed by each second roller 206, 208 towards the output of the tubing
  • Figures 3 A, 3B, 4A, 4B, 5A and 5B illustrate the operation of the first stage 100 and the second stage 200 of the peristaltic pump as the first and second rollers proceed around, i.e., orbit, the peripheries of the first and second disks, respectively.
  • first rollers 106, 108 are located in a first position 310 around the first disk 102.
  • First rollers 106, 108 rotate in a counterclockwise direction, as indicated by arrow 111.
  • the first stage 100 has a flexible tubing 104 that is wrapped around the majority of the outside periphery of the first disk 102.
  • first roller support 128 (not pictured) rotates around the axis 110 and supports roller bracket 112 and roller bracket 114. Roller bracket 112 pivots around pivot 116, while roller bracket 114 pivots around pivot 118. Springs 120 and 122 bias rollers 106 and 108, respectively, to contact the periphery of the first disk 102, the tubing 104, or the periphery of the first disk 102 and the tubing 104.
  • the roller support 128 rotates the first rollers 106, 108 around the first disk 102 in the direction of rotation 111, i.e., counterclockwise, as viewed from the bottom. Because of the irregular shape of the first disk 102, the first rollers 106, 108 travel at different tangential speeds around the periphery of the first disk 108 because the roller support 128 moves at a constant angular rotational speed and the first rollers 106, 108 traverse the periphery of disk 102 at different radii 124, 125, 126, 127 and 129. As used herein, the term "tangential speed" refers to the relative speed between a roller and the surface it is rolling along.
  • the tangential speed of the first rollers 106, 108 on the periphery of the first disk 102 is greater than the tangential speed of the first rollers when they are traversing the periphery of the first disk 102 at radii 125, 126, and 127. Since the first rollers 106, 108 move faster in the areas where the radius is greater, the first rollers 106, 108 move along the flexible tubing 104 in these areas at a greater rate of speed.
  • first rollers 106, 108 are moving along the periphery of disk 102 on portions of the first disk 102 that have a shorter radius, such as radii 126, 127, the first rollers 106, 108 move in these areas at a slower rate of speed along the flexible tubing 104.
  • both first rollers 106, 108 are compressing the flexible tubing 104, and one of the first rollers is moving faster on the periphery of first disk 102 than the other first roller, the fluid trapped in the tubing between first rollers 106 and 108 experiences a pressure increase.
  • the first roller support 128 rotates around the first disk 102 at a constant angular rotational speed
  • the first rollers 106, 108 are biased against the periphery of the first disk 102, the tubing 104, or the periphery of the first disk 102 and the tubing 104, and cause the flexible tubing 104 to experience various states of compression at various locations around the periphery of the first disk 102.
  • Fluid from the intake tubing 101 is drawn into the flexible tubing 104 as the first rollers 106, 108 move in a counterclockwise direction 111 and fully compress the flexible tubing 104. Fluid is thus drawn from the intake tubing 101 and is forced out of the interconnecting tubing 130 and proceeds to the second stage that is illustrated in Figure 2.
  • interconnecting tubing 130 extends from the first stage 100, proceeds to the second disk 202, and is wrapped around part of the periphery of the second disk 202 in a clockwise direction.
  • Disk 102 and second disk 202 may be aligned with each other as discussed herein, although it is to be understood that there may be many other arrangements of the first and second disks that may still provide the same functionality as is discussed herein.
  • both disks may actually be arranged as depicted in Figures 3 A and 3B (side-by-side), but with the second disk and rollers flipped over so that the direction of rotation of the roller support 228 rotates in the same direction as the roller support 128— both roller supports 128 and 228 may be driven by the same drive system and the rollers and tubing may operate in effectively the same way as is described herein with regard to the depicted example.
  • First roller support 128 rotates around the first disk 102 (as shown in Figure 1) synchronously with second roller support 228, which rotates round second disk 202. Consequently, the rotational phase of the first rollers 106, 108 and the second rollers 206, 208 remains constant.
  • the roller supports of the first stage 100 and the second stage 200 rotate in the same direction, even though arrow 111 indicates a counterclockwise rotation and arrow 211 indicates a clockwise rotation. Again, this is because Figures 1 and 3 A are bottom views of the peristaltic pump and Figures 2 and 3B are top views of the peristaltic pump.
  • the second disk 202 has a substantially constant radius 232 (e.g., within ⁇ 1% or ⁇ 5% of round; 1% or less may result in the least amount of pressure variation in the fluid that gets trapped between the second rollers as they orbit the second disk).
  • the flexible tubing 204 is wrapped around the majority of the periphery of the second disk 202 so that the second rollers 206, 208 can compress the flexible tubing 204 along portions of the periphery of the second disk 202 that are exposed to the second rollers 206, 208, such as the portions including locations 314, 315, and 316.
  • the fluid enters the flexible tubing 204 from interconnecting tubing 130 from the first stage 100.
  • Second rollers 206, 208 are mounted on roller brackets 212, 214, respectively. As stated above, roller brackets 212, 214 rotate on pivots 216, 218, respectively, and springs 220, 222 constantly press the second rollers 206, 208 into contact with the periphery of the second disk 202, the tubing 204, or the periphery of the second disk 202 and the tubing 204.
  • first roller 106 is located at position 310 on the outer periphery of the first disk 102 where there is no compression of the tubing 104 because at this location the first disk 102 is configured such that the flexible tubing 104 is not exposed to the pressure of the first roller 106. In fact, the flexible tubing 104 is not even disposed along the periphery of the first disk 102 at location 310.
  • Uptake tubing 101 as described above, provides intake fluid to the flexible tubing 104.
  • the flexible tubing 104 is wrapped around the periphery of the first disk 102 from the uptake tubing 101, counterclockwise around the periphery of the first disk 102 to the interconnecting tubing 130.
  • the flexible tubing 104 will be exposed to, partially exposed to, or not exposed to the pressure of the first rollers 106, 108, which results in the flexible tubing 104 being fully compressed, partially compressed or not compressed, as explained in more detail below.
  • roller 108 contacts the first disk 102 at location 305 and fully compresses the flexible tubing 104.
  • the labels of locations 303, 304, 305 and 306, the flexible tubing 104 indicate that tubing 104 is fully compressed by the first rollers 106, 108 at these locations.
  • locations 308 and 310 there is no compression of the flexible tubing 104 by the first rollers 106, 108.
  • the tubing 104 is partially compressed.
  • the first disk 102 has various length radii.
  • the first disk 102 has longer radii 124 and 129 (e.g., longer radii in at least the regions between positions 310 and 304, in a clockwise direction from position 310) which cause the first rollers 106, 108 to move at a faster tangential speed along the peripheral surface of the first disk 102 at and between these positions.
  • Radii 125, 126, and 127 are shorter than the radii 129, 124, such that the first rollers 106, 108 do not move as quickly along the peripheral surface of the first disk 102 for these radii.
  • first roller 106 moves at a faster tangential speed as it moves in the clockwise direction (with the roller support maintaining a constant rotational speed) from position 310 (having radius 129) to position 303 (having radius 124) than its tangential speed as it moves from position 305 (having radius 125) to position 306 (having radius 126, which in some embodiments may be the same length as radius 126) because the radii at positions 305 and 306 are shorter than the radii between positions 310 and 303.
  • first roller 106 when the first rollers 106, 108 are at locations 310, 305, respectively, first roller 106 is not compressing the flexible tubing 104, while first roller 108 is fully compressing the flexible tubing 104. As such, when first roller 108 moves into the position 305, roller 108 is drawing fluid from the intake tubing 101, since the flexible tubing 104 is not compressed by first roller 106, i.e., at these positions the first roller 106 does not affect the fluid flow within the tubing.
  • First roller 108 continues to draw fluid through the intake tubing 101, as it rotates counterclockwise around the periphery of the first disk 102 through position 306 until first roller 106 starts fully compressing flexible tubing 104 between positions 302 and 303. It should be noted that the first disk 102 and first rollers are configured such that as one first roller moves from position 306 to position 308, that first roller does not stop fully compressing the tubing until after the other first roller is fully compressing the tubing.
  • Figure 3B illustrates the operation of the second stage 200.
  • first rollers 106, 108 are in the locations illustrated in Figure 3 A
  • second rollers 206, 208 are located in the corresponding positions illustrated in Figure 3B on the periphery of the second disk 202.
  • second roller 208 is located at position 311 and is not compressing flexible tubing 204.
  • Second roller 206 is at position 315 and is fully compressing the flexible tubing 204.
  • fluid trapped between first roller 108 at position 305 and the second roller 206 at position 315 is moved by first roller 108 and second roller 206 towards the output tubing 230 through the interconnecting tubing 130 and through the flexible tubing 204.
  • first roller 108 moves from position 305 to 306, it is moving at the same tangential speed as the second rollers 206, 208 because the radius of disk 102 at positions 305 through 308 is substantially the same as the radius of the second disk 202. Accordingly, the first roller 108 pushes (and the second roller 206 pulls at the same rate) and causes the fluid in the tubing 104 to move from position 305 towards the second disk 202 without increasing the pressure of the fluid trapped between first roller 108 and the second roller 206 as they move between these positions (i.e., 305 to 306 and 315 to 316, respectively).
  • Flexible tubing 204 is fluidically connected to the output tubing 230, which delivers fluid to a flow cell an embodiment in which the peristaltic pump is used in a flow cytometer.
  • the output tubing 230 is fluidically connected to a nozzle of a flow cytometer and the output pressure of the output tubing 230 may be governed by the pressure of the fluid within the nozzle.
  • output tubing 230 simply comprises the output of the second stage 200 of the peristaltic pump.
  • Figure 4A is a schematic illustration of the first stage 100 of the peristaltic pump with the first rollers 106, 108 in a second position. First roller 106 has moved from position 310 to position 303 where the first roller 106 is fully compressing the flexible tubing 104.
  • first roller 108 has moved in a counterclockwise direction from position 305, where the first roller 108 was fully compressing the flexible tubing 104, to position 306, where there is still full compression of the first roller 308 on the flexible tubing 104.
  • first roller 106 starts compressing and then fully compresses the flexible tubing 104 which causes a portion of fluid to be trapped between first rollers 106 and 108; this trapped portion of fluid will experience a pressure increase as the first rollers continue to advance while both first rollers fully compress the tubing.
  • the fluid that is trapped between the first roller 108 and the second roller 206 which includes the fluid in the interconnecting tubing 130, is already pressurized to the final output flow pressure. Subsequently, second roller 208 transitions to full compression of the tubing while second roller 206 is fully compressing the tubing.
  • FIG. 4B is a schematic illustration of the second stage 200 of the peristaltic pump.
  • Second roller 208 has moved from position 311, where there was no compression of tubing 204, in a clockwise direction, to position 312, where there is partial compression of tubing 204.
  • second roller 206 has moved from position 315, where there is full compression of tubing 204, to position 316, where there is also full compression of tubing 204.
  • first roller 108 is pushing, i.e., moving, the liquid in tubing 104 through tubing 104, tubing 130, and tubing 204 as it advances from position 305 to position 306 and going at the same tangential speed as second roller 206 because radii 125 and 126 are substantially the same as the radius of the second disk 202. This prevents the pressure of this portion of fluid from increasing and instead causes the pressure to remain substantially constant during movement of this portion of the fluid.
  • Figure 5A is a schematic top view of the first stage 100 of the peristaltic pump illustrating first rollers 106, 108 in a third position.
  • first roller 106 has moved in a counterclockwise direction, as indicated by arrow 111, from position 303, where there was full compression of tubing 104, to position 304, where there is also full compression of the flexible tubing 104.
  • First roller 108 has moved from position 306, where there was full compression of the flexible tubing 104, to position 308, where there is no compression of the flexible tubing 104.
  • the pressure of the fluid trapped between the first rollers 106, 108 as one of the first rollers moves from position 303 to 304 and the other first roller simultaneously moves from position 306 to 308 may increase because the roller moving from position 303 to 304 is moving at a faster tangential speed than the roller moving from position 306 to 308.
  • this pressure increase may be negligible, e.g., if one first roller starts fully compressing the tubing just before the other first roller stops fully compressing the tubing. However, after a first roller stops fully compressing the tubing, e.g., between locations 306 and 308, the fluid trapped between the other first roller and one of the second rollers may be further pressurized as those rollers continue to traverse the periphery of their respective disks— indeed, the bulk of the pressure increase that is experienced by the fluid may occur while the fluid is trapped between one of the first rollers and one of the second rollers.
  • the fluid between the first rollers is moved by the first roller 106 to the interconnecting tubing 130 under pressure and transmitted to the second stage 200 that is illustrated in Figure 5B.
  • a "handof ' may occur between the first rollers and the second rollers such that a portion of the fluid trapped between a first roller located in the region between locations 305 and 308 and a second roller located in the region between locations 315 and 318 is subdivided as another second roller fully compresses the tubing in which the trapped fluid is located.
  • the portion of fluid that is trapped between the two second rollers is thus “handed off from the first stage to the second stage, and the second stage rollers move this handed-off portion of the fluid to the outlet under constant pressure.
  • the other portion of the fluid that is trapped between the first roller and the (recently compressing) second roller is also moved at constant pressure towards the outlet.
  • that first roller stops fully compressing the tubing e.g., such as at location 308
  • the portion of the fluid that was trapped between that first roller and a second roller will experience a pressure decrease as it equalizes with the lower-pressure fluid that was previously trapped between the two first rollers (which may be at or slightly above the intake pressure, depending on how much the pressure increases while such fluid is moved while trapped between the first rollers).
  • the differential tangential speeds of the first roller and the second roller(s) as the first roller travels along the periphery of the first disk having the larger radii then raises the pressure of the fluid trapped between the first roller and the second roller up to the desired outlet pressure.
  • FIG. 5B illustrates the second stage 200 of the peristaltic pump with second rollers 206, 208 in a third position.
  • Second roller 208 has moved from position 312 to position 314, and fully compressed tubing 204 at some location after position 312 and before reaching position 314.
  • Second roller 206 has moved from position 316, where there is full compression, to position 318, where there is no compression of tubing 204.
  • second roller 208 has taken on the task of moving the fluid in tubing 204, while second roller 206 has moved to a position (e.g., position 318) where there is no compression, so that the fluid being moved by second roller 208 can pass through to the output tubing 230.
  • the second stage 200 simply moves the fluid by alternately using second rollers 206, 208 to advance the fluid in tubing 204.
  • the second disk 202 is configured such that full compression of tubing 204 is caused by a second roller moving between positions 312 and 314 before another second roller simultaneously moving between positions 316 and 318 is not fully compressing tubing 204.
  • the peristaltic pump disclosed herein increases the pressure of a portion of fluid in the tubing between a first roller and a second roller by causing that first roller to move at a faster tangential speed around the first disk than that second roller.
  • this pressure increase is caused by the first roller pushing fluid against the second roller, thereby decreasing the length of tubing to contain the same volume of fluid, which causes the tubing to expand in order to accommodate the fluid, and thus increases the pressure of the fluid.
  • the movement of the rollers and configuration of the disks to cause this pressure increase will now be discussed in further detail.
  • Figures 11 A and 1 IB depict the peristaltic pump of Figures 3A and 3B, respectively, and as can be seen, most of the labels have been removed from Figures 3 A and 3B and three shaded sectors in each disk have been added.
  • the first rollers 106, 108 orbit around the periphery of the first disk at a constant angular speed and these first rollers 106, 108 are constantly pressed into contact to with the periphery of the first disk 102, the tubing 104, or the periphery of the first disk 102 and the tubing 104.
  • first roller 106 orbits around disk 102 and reaches position 166 (which is between positions 303 and 304 on Figure 3A, and which are not labeled in Figure 11 A), first roller 106 is fully compressing the tubing 104 and first roller 108 is no longer compressing tubing 104.
  • second roller 208 is at position 266 and is fully compressing tubing 204. Accordingly, a portion of fluid exists between first roller 106 and second roller 208 in tubing 104, 130, and 204; the bulk of the pressure increase in the fluid may occur in this trapped portion of the fluid.
  • First disk 102 in Figure 11 A includes a first angular sector 160 that spans between positions 166 and 168 (position 166 is between positions 303 and 304 and position 168 is between positions 304 and 305 of Figure 3A), includes a first section of the periphery of the first disk 102 (not identified, but corresponding with the portion of the periphery between positions 166 and 168), and has a radius greater than the radius of the second disk 202 and greater than the radius in a second angular sector 162.
  • a first roller such as first roller 106, moves along the first section of the periphery of first disk 102 between positions 166 and 168, that first roller is moving at a first tangential speed and is fully compressing the tubing.
  • Figure 11 A also shows the second angular sector 162 that spans between positions 170 and 172 of the first disk (position 170 corresponds to position 305 and position 172 corresponds to position 306 in Figure 3A), includes a second section of the periphery of the first disk 102 (not identified but corresponding with the portion of the periphery of the first disk between positions 170 and 172), and has a radius substantially equal to the radius of the second disk 202 and smaller than the radius of the first angular sector 160 (this radius corresponds to radius 125 in Figure 3A).
  • a first roller moving along the second section of the periphery of the first disk fully compresses the tubing 104 and moves at a second tangential speed that is less than the first tangential speed.
  • the second rollers 206, 208 orbit around the periphery of the second disk 202 at the same constant angular speed as the first rollers 106, 108.
  • the second rollers are constantly pressed into contact with the periphery of the second disk 202, the tubing 204, or the periphery of the second disk 202 and the tubing 204. Because the second disk has a substantially constant radius that also is substantially equal to the radius of the first disk in at least the second angular sector 162, the second rollers 206, 208 also move at substantially the second tangential speed.
  • the second disk 202 also includes an angular sector 260 that includes a portion of the periphery of the second disk 202 as well as another angular sector 262 that includes another portion of the periphery of the second disk 202.
  • the first disk 102 of Figure 11 A and the second disk 202 of Figure 1 IB and their respective rollers are aligned and configured as described herein in order to increase the pressure of the fluid trapped between a first roller and a second roller.
  • first roller 106 when first roller 106 is at position 166 it is fully compressing the tubing 104, first roller 108 is between positions 172 and 174 such that it is not compressing the tubing 104, second roller 208 is at position 266 and fully compressing the tubing 204, and second roller 206 is partially compressing the tubing 204.
  • first roller 106 moves along the periphery of the first disk 102 within the first angular sector 160 at the first tangential speed
  • second roller 208 simultaneously moves along the angular sector 260 of the second disk at the second tangential speed. Because the first tangential speed is greater than the second tangential speed, first roller 106 pushes the fluid between the first roller 106 and the second roller 208 such that the pressure of this fluid is increased.
  • the volume of the fluid remains the same but the length of tubing to contain the fluid is decreased, thus increasing the pressure in the tubing that expanded to accommodate the same fluid volume in the shorter length of tubing.
  • the pressure increase may cause the fluid to have a pressure increase of about 10 psi. This pressure increase occurs throughout the entire first angular sector 160.
  • first angular sector 160 and the second angular section 162 there may be a transition sector, such as between positions 168 and 170, that that transitions from the radius of the first angular sector 160 and the radius of the second angular sector 162.
  • This varying radius of the transition sector allows the radius of the first disk to reduce from the larger, first radius to the smaller, second radius.
  • the pressure of the fluid may also be caused to increase as a first roller transits through the transition sector, although the rate at which the pressure increases increase will decrease as the first roller transits through the transition sector.
  • first disk 102 functions to increase the pressure of the fluid that is being drawn from the intake tubing 101 by causing the first rollers 106, 108 to move faster than the second rollers on longer-radius portions of the first disk 102 during certain portions of the cycle.
  • the two stage peristaltic pump is capable of pumping fluids with minimal pressure variation at the outlet, which results in little or no pulsing of the fluid at the output tubing 230.
  • Second roller 208 moves from position 312, where there is partial compression, to position 314, where there is full compression.
  • second roller 208 compresses the tubing, which displaces the same volume as the volume that is created when second roller 206 moves from position 316 to position 318. In this manner, a constant pressure is maintained.
  • the first disk 102 may have a first nominal radius throughout at least part of the first angular sector 160 and a second a second nominal radius throughout the second angular sector 162, the second disk 202 may have the second nominal radius, and the first nominal radius may be larger than the second nominal radius.
  • the first roller 106 may be caused to move along a third angular sector 164 of the first disk 102 that is adjacent to the second angular sector 162 and spans between points 172 and 174.
  • This third angular sector 164 may be configured to cause the first rollers to move along a third section of the periphery of the first disk at the second tangential speed (because the third angular sector 164 has substantially the same radius as the second angular sector 162 and the second disk 202).
  • This third angular sector 164 may also be configured to cause the first roller to fully compress the tubing 104 at at least the beginning of the third angular sector 164 and to cause the one first roller 106 to not compress the tubing 104 at at least the end of the third angular section 164.
  • the first roller 108 and 106 are fully compressing the tubing from positions 166 to at least 172.
  • that first roller transitions from fully compressing the tubing 104 to not compressing the tubing 104.
  • the second roller is moving through a different angular sector 264 at the second tangential speed.
  • This different angular sector 264 spans between positions 272 and 274 which correspond to positions 316 and 318, respectively. As such, when a second roller traverses this angular section 264, it is fully compressing the tubing 204 at position 272 and not compressing the tubing 204 at position 274.
  • the first disk 102 may be configured to cause another first roller to fully compress tubing 104 before the first roller in the third angular sector stops fully compressing the tubing in the third angular sector, such as fully compressing the tubing 104 at position 303 of Figure 3 A, as described above.
  • the second disk 202 may also be configured to cause another second roller to fully compress the tubing 204 against the second disk 202 before causing the one first roller to not fully compress the tubing 104 in the third angular sector 164.
  • second roller 208 may fully compress tubing 204 at about position 266 on the second disk 202 before first roller 108 is not fully compressing tubing 104.
  • the second disk 202 may also be configured to cause a second roller, such as second roller 206, to fully compress the tubing 204 when one first roller, such as first roller 108, is at least at the beginning of the third section of the periphery of the first disk 102 (i.e., the beginning of the fourth angular sector 164) and not to compress the tubing 204 when first roller 108 is at the end of the third section of the periphery of the first disk 102.
  • a second roller such as second roller 206
  • first roller 108 when first roller 108 is at position 172 it is fully compressing tubing 104 and roller 206 is simultaneously at position 272 and fully compressing tubing 204; when first roller 108 is at position 174 and not compressing tubing 104, second roller 206 is simultaneously at position 274 and is not compressing tubing 204.
  • the second disk may be configured such that the other second roller is fully compressing tubing between positions 312 and 314 (as labeled in Figure 3 A) before the second roller is not compressing tubing 204 in third angular sector 264.
  • Figure 6 is a cross-sectional view of disk 102 and a first roller 602. Although Figures 6 through 9 are shown with the first disk 102, such embodiments may be equally applicable to the second disk. As shown on Figure 6, the outer periphery of the first disk 102 has a trough 606 (i.e., recess). Where the trough 606 has a first depth 610, as shown in Figure 6, the flexible tubing 104 is not compressed, since the first roller 602 rides along the outer, or peripheral portions of the first disk 102 and does not compress the flexible tubing 104.
  • trough 606 i.e., recess
  • the flexible tubing has an opening 608 in this state that is not compressed and is fully open, so that fluid can easily flow through the flexible tubing 104 as a result of the first depth 610 of the trough 606 at this location on the periphery of the first disk 102.
  • the tubing 104 has a nominal outer diameter in an undeformed state and trough 606 is configured such that the first depth 610 substantially matches this nominal outer diameter so that the first roller 602 does not compress the tubing 104.
  • the first roller 602 rolls along the outer peripheral surface of the first disk 102 at the edges of the trough 606 and rotates on the roller shaft 604.
  • Figure 7 is a cross-sectional view of the first disk 102, first roller 602, and flexible tubing 104 at a different location along the periphery of the first disk 102.
  • the trough 606 is not as deep as the trough 606 in Figure 6, i.e., the first depth 610 in Figure 7 is less than the first depth 610 depicted in Figure 6.
  • the surface of the first roller 602 contacts the flexible tubing 104 and causes the flexible tubing 104 to be partially compressed in the trough 606.
  • the first roller 602 is rolling along the outer peripheral surface of the first disk 102 and rotating about roller shaft 604, as illustrated in Figure 7.
  • Figure 8 is a cross-sectional view of the first disk 102, first roller 602, and the flexible tubing 104 at another location along the periphery of the first disk 102. As illustrated in Figure 8, the trough 606 is not as deep as the trough 606 in Figure 7.
  • the trough, or first recess, 606 has a first depth 610 that is less than the nominal outer diameter of the tubing, thereby causing the tubing 104 to extend past the periphery of the first disk 102 such that the first roller 602 fully compresses the tubing 104 in the trough 606 when the surface of the first roller 602 contacts the flexible tubing 104.
  • the first depth 610 would be less than or equal to twice the wall thickness of the tubing in order to cause such full compression.
  • Tubing that is "fully compressed,” as the term is used herein, is tubing htat has been squashed or compressed to the point where no fluid is able to pass the point of compression within the tubing at the operating pressures utilized.
  • Opening 608 is fully closed. Again, the first roller 602 is rolling along the outer peripheral surface of the first disk 102 around roller shaft 604, as illustrated in Figure 8. Since the flexible tubing is fully compressed, the opening 608 is completely closed off so that no fluid can flow through the opening 608 in the flexible tubing 104.
  • FIG 9 illustrates another embodiment of the manner in which the first roller 602 can be used to compress the flexible tubing 104 using an adjustment plate 902.
  • adjustment plate 902 is anchored to disk 102 by adjustment screws 906; the adjustment plate may be considered to be part of the first disk 102.
  • the adjustment screws 906 extend through the openings 904 in the adjustment plate 902 and are screwed into the first disk 102.
  • Other types of connectors could also be used that are well known in the art.
  • the first roller 602, which rotates on roller shaft 604 rests on the outer surface of the adjustment plate 902.
  • the adjustment plate 902 can be used to provide adjustment as to the location and amount which the first roller 602 compresses the flexible tubing 604.
  • the adjustment plate may extend past a portion of the periphery of the first disk 102, thereby effectively extending the periphery of the first disk 102, and thereby cause the first roller 602 to be in contact with the first adjustment plate 902 and offset from the periphery of the first disk 102 such that the first roller 602 partially compresses the tubing 104.
  • this adjustment plate may form part of the trough 606, i.e., recess.
  • the opening 608 is partially open.
  • the opening 608 would be fully closed if the first roller 602 was sitting on the peripheral edge of the first disk 102. In this manner, the pressure of the fluid can be adjusted, as well as the location where fluid can flow along the disk. By adjusting the radial location of the adjustment plate, the location where the roller fully compresses the flexible tubing can be adjusted, which allows both the pressure generated in the compression phase and the alignment of roller 206 and 208' s transition to be adjusted.
  • FIG 10 is a schematic perspective view of the peristaltic pump 1000.
  • second stage 200 is mounted directly over and aligned with the first stage 100.
  • Pulley 1002 drives pulley 1012, with belt 1008.
  • Pulley 1012 is mounted in top plate 1016 and drives the rotation of the first stage 100.
  • pulley 1004 drives belt 1010, which, in turn, drives pulley 1014; pulley 1014 drives the rotation of the second stage 200.
  • Pulley 1002 and 1004 are connected to each other through a common shaft that proceeds through the center of both pulley 1002 and 1004 thus keeping the rotation of pulleys 1002 and 1004, and therefore stages 100 and 200 synchronized.
  • the common shaft between pulleys 1002 and 1004 is driven by motor 1006 and a motor pulley, belt, and shaft pulley (not shown). In this manner, the rotation of the support arms of stage one and stage two is synchronized.
  • Bottom plate 1018 provides the structural support for pulley 1014.
  • Columns 1020, 1022 provide structural support for various portions of the peristaltic pump 1000.
  • Motor 1006 drives the shaft that connects the pulleys 1002, 1004, which provides the rotational force to drive the peristaltic pump 1000. Accordingly, the shafts connecting pulleys 1002, 1004 and 1012, 1014 provide synchronization between the first stage 100 and the second stage 200 of the peristaltic pump. Further, since the two stages are aligned and connected in the manner shown, a compact design is provided for the peristaltic pump 1000.
  • the embodiments disclosed therefore provide a peristaltic pump 1000 with little or no pulsing of the output fluid at the desired output pressure. Disks are used that have varying radii that allow the fluid to be pre-pressurized in the first stage and subsequently pumped to an output by the second stage, resulting in little or no variations in output pressure of the output fluid.
  • the fluid that is pumped by the peristaltic pump 1000 can be either a liquid or gas, or a mixture of liquid and gas.
  • two rollers are illustrated in the various embodiments, three or more rollers can be used in either stage one and/or stage two.

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  • Engineering & Computer Science (AREA)
  • Mechanical Engineering (AREA)
  • General Engineering & Computer Science (AREA)
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Abstract

L'invention concerne un procédé et un appareil pour pomper un fluide à travers une tubulure. Le procédé met en œuvre le fait de mettre en orbite des premiers rouleaux autour de la périphérie d'un premier disque à une première vitesse tangentielle dans un premier secteur angulaire et à une seconde vitesse tangentielle plus lente dans un second secteur angulaire, le fait de mettre en orbite des seconds rouleaux autour de la périphérie d'un second disque à la seconde vitesse tangentielle, et l'augmentation de la pression de fluide dans une tubulure entre un premier rouleau et un second rouleau par le fait d'amener le premier rouleau à comprimer complètement la tubulure à la première vitesse tangentielle et d'amener simultanément le second rouleau à comprimer complètement la tubulure à la seconde vitesse tangentielle. L'appareil comprend un premier disque avec un creux dans sa périphérie, le premier secteur angulaire avec un premier rayon nominal, et un second secteur angulaire avec un second rayon nominal ; et un second disque avec le premier rayon nominal et un creux dans sa périphérie.
EP16839908.7A 2015-08-21 2016-08-19 Pompe péristaltique de délivrance d'échantillons continue Active EP3337977B1 (fr)

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US11460021B2 (en) * 2017-06-29 2022-10-04 Matthews International Corporation Fluid delivery system and method
WO2023037334A1 (fr) 2021-09-13 2023-03-16 Ecole Polytechnique Federale De Lausanne (Epfl) Système et procédé de profilage phénotypique de cellule unique et d'encapsulation de gouttelettes de l'ordre du nanolitre déterministe et ensembles de consortiums de gouttelettes déterministes

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SG2014010284A (en) * 2011-08-17 2014-03-28 Nestec Sa Linear peristaltic pump
JP2013072287A (ja) * 2011-09-26 2013-04-22 Daiichi Techno Co Ltd スクイズポンプ、弾性チューブ接続管
US9291159B2 (en) * 2013-05-30 2016-03-22 Novartis Ag Pump head with independently sprung offset picoting rollers
CN103423134B (zh) * 2013-08-30 2015-07-29 常州远望流体科技有限公司 微型蠕动泵

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CN107923380B (zh) 2020-02-07
CN107923380A (zh) 2018-04-17
EP3337977B1 (fr) 2020-01-29
EP3337977A4 (fr) 2019-01-02

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