EP3186629A1 - Verfahren zum bestimmen der konzentration von glucoraphanin und/oder von sulforaphan in einer pflanze - Google Patents
Verfahren zum bestimmen der konzentration von glucoraphanin und/oder von sulforaphan in einer pflanzeInfo
- Publication number
- EP3186629A1 EP3186629A1 EP15754115.2A EP15754115A EP3186629A1 EP 3186629 A1 EP3186629 A1 EP 3186629A1 EP 15754115 A EP15754115 A EP 15754115A EP 3186629 A1 EP3186629 A1 EP 3186629A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- sample
- plant
- glucoraphanin
- extract
- measurement signal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- RUQCCAGSFPUGSZ-OBWQKADXSA-N Glucoraphanin Natural products C[S@](=O)CCCCC(=NS(=O)(=O)O)S[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O RUQCCAGSFPUGSZ-OBWQKADXSA-N 0.000 title claims abstract description 67
- GMMLNKINDDUDCF-JRWRFYLSSA-N [(2s,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl] (1e)-5-[(r)-methylsulfinyl]-n-sulfooxypentanimidothioate Chemical compound C[S@@](=O)CCCC\C(=N/OS(O)(=O)=O)S[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O GMMLNKINDDUDCF-JRWRFYLSSA-N 0.000 title claims abstract description 67
- 238000000034 method Methods 0.000 title claims abstract description 40
- SUVMJBTUFCVSAD-UHFFFAOYSA-N sulforaphane Chemical compound CS(=O)CCCCN=C=S SUVMJBTUFCVSAD-UHFFFAOYSA-N 0.000 title claims description 62
- SUVMJBTUFCVSAD-JTQLQIEISA-N 4-Methylsulfinylbutyl isothiocyanate Natural products C[S@](=O)CCCCN=C=S SUVMJBTUFCVSAD-JTQLQIEISA-N 0.000 title claims description 30
- 229960005559 sulforaphane Drugs 0.000 title claims description 30
- 235000015487 sulforaphane Nutrition 0.000 title claims description 30
- 239000000284 extract Substances 0.000 claims abstract description 71
- 238000010521 absorption reaction Methods 0.000 claims abstract description 59
- 238000005259 measurement Methods 0.000 claims abstract description 57
- 239000000126 substance Substances 0.000 claims abstract description 21
- 239000002904 solvent Substances 0.000 claims abstract description 19
- 239000003960 organic solvent Substances 0.000 claims abstract description 10
- 239000000470 constituent Substances 0.000 claims abstract description 6
- 241000196324 Embryophyta Species 0.000 claims description 73
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical group OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 63
- 102000004190 Enzymes Human genes 0.000 claims description 18
- 108090000790 Enzymes Proteins 0.000 claims description 18
- 238000003776 cleavage reaction Methods 0.000 claims description 18
- 230000007017 scission Effects 0.000 claims description 18
- 238000010790 dilution Methods 0.000 claims description 16
- 239000012895 dilution Substances 0.000 claims description 16
- 238000011481 absorbance measurement Methods 0.000 claims description 12
- 235000011299 Brassica oleracea var botrytis Nutrition 0.000 claims description 9
- 235000017647 Brassica oleracea var italica Nutrition 0.000 claims description 9
- 240000003259 Brassica oleracea var. botrytis Species 0.000 claims description 9
- 238000009499 grossing Methods 0.000 claims description 9
- FGUUSXIOTUKUDN-IBGZPJMESA-N C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 Chemical compound C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 FGUUSXIOTUKUDN-IBGZPJMESA-N 0.000 claims description 4
- 240000007594 Oryza sativa Species 0.000 claims 1
- 235000007164 Oryza sativa Nutrition 0.000 claims 1
- 235000009566 rice Nutrition 0.000 claims 1
- 238000002474 experimental method Methods 0.000 description 16
- 235000007849 Lepidium sativum Nutrition 0.000 description 9
- 244000211187 Lepidium sativum Species 0.000 description 9
- 238000001914 filtration Methods 0.000 description 6
- 238000011088 calibration curve Methods 0.000 description 5
- 238000000862 absorption spectrum Methods 0.000 description 4
- 208000027418 Wounds and injury Diseases 0.000 description 3
- 238000002835 absorbance Methods 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 208000014674 injury Diseases 0.000 description 3
- 238000001228 spectrum Methods 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 229920006395 saturated elastomer Polymers 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- 229920000997 Graphane Polymers 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- SDIXRDNYIMOKSG-UHFFFAOYSA-L disodium methyl arsenate Chemical compound [Na+].[Na+].C[As]([O-])([O-])=O SDIXRDNYIMOKSG-UHFFFAOYSA-L 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000004811 liquid chromatography Methods 0.000 description 1
- 238000004949 mass spectrometry Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 244000062645 predators Species 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/0098—Plants or trees
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/31—Brassicaceae or Cruciferae (Mustard family), e.g. broccoli, cabbage or kohlrabi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/33—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using ultraviolet light
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/02—Food
- G01N33/025—Fruits or vegetables
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/12—Circuits of general importance; Signal processing
Definitions
- the invention relates to a method for determining the concentration of glucan-5-coraphanin in a plant, wherein
- the sample is contacted with a polar, uncharged organic solvent and comminuted such that glucoraphanin contained in the sample and other plant substances contained in the sample are dissolved in the oil solvent, and
- the invention further relates to a method for determining the concentration of bioavailable sulforaphane in a plant, which plant contains glucoraphanin and a spatially separated cleavage enzyme, upon contact with the glucoraphanin, the plant forms sulforaphane.
- Such a method for determining the concentration of glucoraphanin is known in the art.
- one of the plants to be investigated is prepared and comminuted in a solvent containing acetone such that glucoraphanine contained in the sample is dissolved in the solvent.
- the thus-obtained extract is first clarified and purified, and then a liquid chromatography is performed, in which the extract is transported under high pressure through a column. Along the column, different molecules contained in the extract are separated from each other.
- the column is irradiated with UV light, which is absorbed by the molecules.
- the presence of the glucuraphanine molecules at a predetermined position of the column is detected by means of a UV-sensitive optical sensor.
- the glucoraphanin molecules are then quantified by mass spectrometry in a further process step.
- Bokkolicress has a special high content of glucoraphanin and also contains a spatially separate cleavage enzyme which contacts the glucoraphanin upon injury of the plant.
- the glucoraphanin is split into sulforaphane and a sugar residue.
- the sulforaphane is actually used to prevent predators from the plant.
- Studies have shown, however, that foods containing sulforaphane in sufficient concentrations are also beneficial to human health. Therefore, boccolic cress is preferred for clinical trials.
- concentration of glucoraphanin and, in particular, that of the bioavailable sulforaphane in boccolic cress must be known as accurately as possible.
- concentration of the bioavailable sulforaphane is understood to mean the concentration of the sulforaphane which the plant itself can form when the glucoraphanin contained in the plant comes into contact with the cleavage enzyme also present in the plant.
- the previously known method for determining the concentration of Glucoraphanins has the disadvantage that its naturalfijhrung is relatively cumbersome and time-consuming and that large equipment is required, which are usually present only in a entspre ⁇ accordingly equipped laboratory. It is also unfavorable that the required analyzers are maintenance-intensive and that suitably qualified personnel are required to carry out the process.
- a method for determining the concentration of bioavailable sulforaphane of the type mentioned is not known to the knowledge of the applicants. It is therefore the object to provide a method of the type mentioned above, which makes it possible to determine the concentration of glucoraphanin a plant in a simple manner .. Furthermore, the object is to provide a method which is a simple determination of the concentration of bioavailable sulforaphane in a plant allows.
- a sample of the plant is provided, the sample is contacted with a polar, uncharged organic solvent and comminuted such that eggucoraphanin contained in the sample and other vegetable matter contained in the sample are dissolved in the solvent,
- a UV absorption measurement of the clarified extract containing glucoraphanin and the further plant substances is carried out, in which an absorption measurement signal is recorded for a first wavelength range extending from 200 nm to 270 nm,
- the second derivative of the absorbance measurement signal is formed for a second wavelength range between 240 nm and 250 nm, and the magnitude of the minimum of the second derivative in the second wavelength range is determined and a concentration value for the glucoraphanine concentration is determined as a function of this magnitude.
- the method according to the invention can be carried out in a simple manner with little expenditure on apparatus, for example by means of a corresponding hand-held device. It is even possible to carry out the process at the place of cultivation of the plant to be examined, such as in a field or in a greenhouse. Since the method according to the invention can easily be automated, it can also be carried out reliably and quickly by persons who have no special qualifications in handling analysis devices.
- the absorption measurement signal is smoothing filtered and the second derivative is formed from the smoothing filtered absorption measurement signal.
- the smoothing filtering which may include a low-pass filtering, can suppress background noise and the like disturbances, so that they do not or only significantly affect.
- a smoothing filter for example, a Savitzky-Golay filter can be used.
- the plant contains in addition to the glucoraphanin a spatially separated therefrom cleavage enzyme, in its contact with the glucoraphanin the plant forms sulforaphane, wherein
- a) a first sample of the plant is provided and, with the features of claim 1, a first concentration value for the concentration of glucuraphanine in the first sample is determined,
- the difference between the determined for the first sample concentration value and the concentration value determined for the second sample is determined.
- the sample is mixed with the solution brought into contact, so that then only that part of the glucoraphanine originally present in the sample dissolves in the solvent, which was not cleaved by the cleavage enzyme in Sulföraphan and the sugar residue and evaporated.
- the concentration of glucanaphanine is measured, and the difference is formed from the concentration values thus obtained.
- This difference corresponds to the concentration of glucoraphanin from which sulpho-graphane can be formed on contact with the cleavage enzyme contained in the sample, ie the concentration of the bioavailable sulforaphane of the plant.
- step d) of claim 1 and / or in step f) of claim 1 absorption measurement signal
- step d) of claim 1 is repeated with the dilute extract, wherein the dilution factor is chosen such that the absorbance measurement signal of the dilute extract does not exceed the limit and steps e)
- the limit value is preferably selected such that the measurement signal detected during the absorption measurement is smaller than the value at which the absorption measurement 5 comes into the limit.
- the threshold is preferably between 2 and 3.
- the first sample is contacted with a polar, uncharged organic solvent and comminuted such that glucoraphanin contained in the sample and other substances contained in the sample are dissolved in the solvent; 0 c) from the thus obtained first extract unresolved convinced to clarify the first extract,
- the second sample is comminuted such that the glucoraphanin comes into contact with the cleavage enzyme
- Runaway ⁇ performs a first measurement of UV absorbance of the clarified first, the glucoraphanin and the extract contained other plant materials, wherein for a first wavelength range between 200 nm and 270 nm, a first absorption measurement signal is detected,
- the amount of the minimum of the second derivative of the difference signal in the second wavelength range is determined and a concentration value for the concentration of the sulphonaphane is determined as a function of this amount.
- the concentration value for the concentration of the plant-available bioavailable sulforaphane can therefore also be determined by first forming the difference between the absorption measurement signals measured during the first and second experiments, and then forming the second derivative of the difference signal and in the wavelength range between 240 nm and 250 nrm the amount of the minimum of the second derivative is determined. Also in this method can be carried out directly with the extracts prepared in the experiments, the UV absorption measurement without the extracts must be previously purified. The process is simple to perform and can be easily automated.
- the measured in step i) of claim ⁇ absorption measurement signal is compared with a limit, wherein in the event that the absorption measurement signal exceeds the limit, the extract diluted according to a dilution factor and step i) of claim ⁇ with the diluted Extract is repeated, wherein the dilution factor is selected such that the absorbance measurement signal of the diluted extract does not exceed the limit, and that in step k) of claim ⁇ as the first absorption measurement signal multiplied by the dilution factor absorption signal of the diluted extract is used.
- the limit value is preferably selected such that the measurement signal detected in the absorption measurement is smaller than the value at which the absorption measurement comes within the limit.
- the limit value is preferably between 2 and 3.
- the absorption measurement signal and / or the difference signal is smooth-filtered and if the second derivative is formed from the smoothing-filtered difference signal.
- the smoothing filtering which may include low-pass filtering, can suppress background noise and the like disturbances, so that they do not or only insignificantly affect the measuring accuracy.
- the plant is broccoli cress. This has a particularly high content of glucoraphanin and bioavailable sulforaphane
- methanol is preferably used as the solvent.
- the solvent can then be disposed of environmentally friendly after its use.
- FIG. 1 is a graphical representation of an absorption measurement signal ⁇ ( ⁇ ) and its first derivative da (A) / dA and its second derivative d 2 a (A) / dA 2 , wherein the abscissa on the wavelength in nanometers and on the Or ⁇ dinate left the absorption or its first derivative, and on the right Ordi ⁇ nate the second derivative of absorbance are plotted, and
- Fig. 2 is a graph of a calibration curve, wherein the abscissa on the second derivative of the absorption and on the ordinate the concentration of glucoraphanin are applied in an extract.
- a first sample of broccoli cress which has a mass of 251 mg.
- the broccoli cress contains glucoraphanin and a spatially separate cleavage enzyme, which upon contact with the glucoraphanin sulforaphane is formed.
- the broccoli cress contain other plant substances.
- the first sample is mixed with a first amount of methanol having a mass of 1700 mg and pureed in such a way that the glucoraphanin contained in the first sample and the other plant substances are dissolved in the solvent.
- a first amount of methanol having a mass of 1700 mg and pureed in such a way that the glucoraphanin contained in the first sample and the other plant substances are dissolved in the solvent.
- undissolved plant constituents are clarified by filtering and / or centrifuging.
- 18 mg are removed and mixed with a second amount of methanol having a mass of 759 mg, i.
- the dilution factor is determined with the aid of an expectation value for the glucoraphanin concentration in such a way that an absorbance meter used in a UV absorption measurement carried out in a further process step to be described below would be optimally controlled if the concentration of glucoraphanine in the sample was of the expected value would agree.
- the absorption measurement signal ⁇ ( ⁇ ) shown in FIG. 1 is detected for a first wavelength range which extends from 200 nm to 270 nm.
- the absorption measurement signal ⁇ ( ⁇ ) has a resolution of 0.2 nm.
- the Absorp ⁇ tion measurement signal ⁇ ( ⁇ ) is smoothed with a Savitzky-Golay filter, for example, about from 21 to 5 31 points of the spectral resolution.
- the experiment i o is aborted and a new first attempt is made, in which a larger dilution factor is chosen than the first attempt.
- the abovementioned method steps are carried out in a corresponding manner for the new experiment.
- the absorption measurement signal ⁇ ( ⁇ ) is mathematically derived twice in a second wavelength range extending from 240 nm to 250 nm.
- the first derivative da (A) / dA and the second derivative d 2 a (A) / dA 2 are shown graphically in FIG. These can be calculated numerically using a microcomputer. If necessary, the dual derivative can also be done in one step together with the Savitzky-Golay smoothing.
- the magnitude of the minimum 5 M of the second derivative d 2 a (A) / dA 2 of the absorption measurement signal a (A) is determined.
- the minimum M is at a wavelength AM of about 248 nm and the magnitude of the minimum M is 9.37 x 10 -5.
- the absorption line is Therefore, the absorbance measurement signal a (A) 0 can not be directly used for the determination of the concentration of glucoraphanine in the first sample.
- the second derivative shows a distinct local minimum at 246 nm, followed by a local maximum lying in the wavelength range between 241 nm and 203 nm.
- the background spectrum that the makes up the largest part of the spectrum becomes negligibly small by the second derivative and focusing on a small, lying between 200 nm and 270 nm region of the spectrum.
- a concentration value for the glucoraphanine concentration c of the diluted extract is assigned to it using the calibration curve shown in FIG. This is 0.54 ⁇ g / ml.
- y (l) of glucoraphanin in the sample is then given as follows: y (1) - c ( ⁇ ) m ( extract ü + m ( meth ° 12) m ( methanol> l K l62, ⁇ 5 mg / 100 g,
- the calibration curve shown in FIG. 2 can be determined by means of experiments which initially provide a number of reference extracts which contain glucoraphanine in different concentrations.
- the glucoraphenine concentrations of the reference extracts are preferably determined by weighing a predetermined amount of glucoraphanine and then dissolving it in a predetermined amount of methanol.
- the amount of methanol can also be determined by weighing.
- the glucoraphanin concentration of the individual reference extracts then corresponds in each case to the quotient of the amount of glucoraphanin and the sum of the amount of glucoraphanin and the amount of methanol.
- the amount or value of the minimum M of the second derivative of the absorption measurement signal in the second wavelength range is determined with the aid of the method according to the invention and assigned to the concentration value of the relevant reference extract.
- the value combinations obtained in this way are stored as calibration parameters for determining the glucoraphanine concentration in future experiments, for example in a data memory of a microcomputer.
- the calibration curve may be in the form of a calculation rule, such as a straight line equation, or in the form of sampling points, each comprising the amount or value of the minimum and a concentration of glucoraphanin. Intermediate values between two interpolation points can be interpolated if necessary.
- a second sample is provided which has a mass of 250 mg.
- the second sample is pureed such that the glucoraphanin contained in the first sample contacts the cleavage enzyme to form sulforaphane.
- a predetermined period of time is waited, which is dimensioned such that the sulforaphane contained in the second sample can substantially completely evaporate.
- the second sample is so mixed with a third amount of methanol in the amount of 1 772 mg that still contained in the second sample glucoraphanin, which was not cleaved in sulforaphane, and the other, still contained in the second sample plant substances in the methanol be solved. From the extract thus obtained, undissolved plant constituents are clarified by filtering and / or centrifuging.
- the already mentioned UV absorption measurement of the clarified extract containing the glucoraphanin and the further plant substances is carried out, in which an absorption measurement signal is detected in the first wavelength range. If the measured absorption spectrum in the first wavelength range has readings above 2.5, it is assumed that the absorption meter has become saturated. In this case, the second attempt is aborted and a new second attempt is made using a larger dilution factor.
- the absorption measurement signal in the second wavelength range is mathematically derived twice.
- the magnitude of the minimum of the second derivative of the absorbance measurement signal at the wavelength AM of about 248 nm is determined.
- the amount of the minimum is 5.29 x 10 "5
- This value is assigned a concentration value for the glucoraphanine concentration c of the diluted extract on the basis of the calibration curve shown in FIG. This is 0.31 g / ml.
- concentration y (2) of the glucoraphanin in the sample, which is not cleaved into sulfobraphane upon injury of the sample, is then given as follows: m ⁇ extract, 2) + m (methanol, 4) m (methanol, 3)
- m extract,! m (Viobe, 2) where: m (sample, 2) the amount of sample of the second experiment in [mg], m (methanol, 3) the third amount of methanol [mg], m (methanol, 4) the fourth amount of methanol [mg],
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Abstract
Description
Claims
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE102014012367.7A DE102014012367B3 (de) | 2014-08-25 | 2014-08-25 | Verfahren zum Bestimmen der Konzentration von Glucoraphanin und/oder von Sulforaphan in einer Pflanze |
PCT/EP2015/001652 WO2016029998A1 (de) | 2014-08-25 | 2015-08-11 | Verfahren zum bestimmen der konzentration von glucoraphanin und/oder von sulforaphan in einer pflanze |
Publications (1)
Publication Number | Publication Date |
---|---|
EP3186629A1 true EP3186629A1 (de) | 2017-07-05 |
Family
ID=53782744
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP15754115.2A Withdrawn EP3186629A1 (de) | 2014-08-25 | 2015-08-11 | Verfahren zum bestimmen der konzentration von glucoraphanin und/oder von sulforaphan in einer pflanze |
Country Status (4)
Country | Link |
---|---|
US (1) | US10444212B2 (de) |
EP (1) | EP3186629A1 (de) |
DE (1) | DE102014012367B3 (de) |
WO (1) | WO2016029998A1 (de) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
SG11201701015QA (en) * | 2014-08-29 | 2017-03-30 | Univ Tohoku | Optical concentration measuring method |
DE102019118171A1 (de) * | 2019-07-04 | 2021-01-07 | Endress+Hauser Conducta Gmbh+Co. Kg | Verfahren zum Betreiben eines automatischen Analysegeräts und ein automatisches Analysegerät |
Family Cites Families (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5725895B1 (en) * | 1995-09-15 | 2000-10-10 | Hopkins J School Of Medicine | Method of preparing food product from cruciferous seeds |
US6242018B1 (en) * | 1997-04-11 | 2001-06-05 | Johns Hopkins School Of Medicine | Cancer Chemoprotective food products |
WO2006102236A1 (en) * | 2005-03-18 | 2006-09-28 | Caudill Seed Inc. | Cancer chemoprotective compositions and natural oils and methods for making same |
US20080312164A1 (en) * | 2006-07-14 | 2008-12-18 | Rajski Scott R | Isothiocyanates and glucosinolate compounds and anti-tumor compositions containing same |
US20080131578A1 (en) * | 2006-10-27 | 2008-06-05 | Caudill Seed and Warehouse Company, Inc. | Food or drink products, supplements or additives produced from high glucoraphanin-containing broccoli variety 'hopkins' |
HUE046151T2 (hu) * | 2007-01-23 | 2020-02-28 | Pharmagra Labs Inc | Stabilizált szulforafán |
NZ581988A (en) * | 2007-07-19 | 2012-06-29 | Univ Oregon State | Method for converting glucosinolate |
US8927007B2 (en) * | 2010-09-17 | 2015-01-06 | Paul Talalay | Formulations for treatment with glucosinolates |
MX2011005064A (es) * | 2011-05-13 | 2012-11-19 | Ceuticos De Vida S A De C V | Metodo de cuantificación de sulforafano por cromatografía líquida de alta resolución en plantas crucíferas. |
FR2977161B1 (fr) * | 2011-07-01 | 2013-07-19 | Sojasun Technologies | Compositions pour le traitement ou la prevention du cancer de la prostate a base d'extrait de graines de brocoli. |
EP3062802B1 (de) * | 2013-11-01 | 2023-11-15 | Rutgers, The State University of New Jersey | Pflanzenextrakte aus moringa oleifera und verfahren zur herstellung |
US20180332881A1 (en) * | 2017-05-17 | 2018-11-22 | Mead Johnson Nutrition Company | Preterm infant formula containing butyrate and uses thereof |
-
2014
- 2014-08-25 DE DE102014012367.7A patent/DE102014012367B3/de active Active
-
2015
- 2015-08-11 WO PCT/EP2015/001652 patent/WO2016029998A1/de active Application Filing
- 2015-08-11 EP EP15754115.2A patent/EP3186629A1/de not_active Withdrawn
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2017
- 2017-02-24 US US15/441,307 patent/US10444212B2/en active Active
Non-Patent Citations (2)
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Also Published As
Publication number | Publication date |
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US20170205384A1 (en) | 2017-07-20 |
WO2016029998A1 (de) | 2016-03-03 |
US10444212B2 (en) | 2019-10-15 |
DE102014012367B3 (de) | 2015-08-27 |
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