EP2934750B1 - Utilisation de tubes d'échantillons et de bouchons vissés pour stockage à froid de matériel biologique - Google Patents

Utilisation de tubes d'échantillons et de bouchons vissés pour stockage à froid de matériel biologique Download PDF

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Publication number
EP2934750B1
EP2934750B1 EP13821457.2A EP13821457A EP2934750B1 EP 2934750 B1 EP2934750 B1 EP 2934750B1 EP 13821457 A EP13821457 A EP 13821457A EP 2934750 B1 EP2934750 B1 EP 2934750B1
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EP
European Patent Office
Prior art keywords
screw
tube
screw cap
section
use according
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Application number
EP13821457.2A
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German (de)
English (en)
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EP2934750A1 (fr
Inventor
Bernd Roman KRANZ
Sven MÜHLFRIEDEL
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Helmholtz Zentrum Muenchen Deutsches Forschungszentrum fuer Gesundheit und Umwelt GmbH
Greiner Bio One GmbH Germany
Original Assignee
Helmholtz Zentrum Muenchen Deutsches Forschungszentrum fuer Gesundheit und Umwelt GmbH
Greiner Bio One GmbH Germany
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Publication of EP2934750A1 publication Critical patent/EP2934750A1/fr
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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D41/00—Caps, e.g. crown caps or crown seals, i.e. members having parts arranged for engagement with the external periphery of a neck or wall defining a pouring opening or discharge aperture; Protective cap-like covers for closure members, e.g. decorative covers of metal foil or paper
    • B65D41/02—Caps or cap-like covers without lines of weakness, tearing strips, tags, or like opening or removal devices
    • B65D41/04—Threaded or like caps or cap-like covers secured by rotation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082—Test tubes per se
    • B01L3/50825—Closing or opening means, corks, bungs
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50853—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates with covers or lids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B01L2300/042—Caps; Plugs

Definitions

  • the present invention relates to a screw tube for biomaterial, a screw cap for closing such a screw tube for biomaterial and a system with a screw tube and a screw cap. Furthermore, the present invention relates to a storage device for receiving a plurality of such screw tubes.
  • biomaterial is introduced into so-called tubes or tubes for this purpose. These tubes or tubes are then closed with a lid.
  • the tubes and the lid may be configured as a screw tube and screw cap.
  • the tubes are placed in corresponding storage devices or racks. Typically, these are then cooled for storage. Depending on the individual study, the cooling temperature is different. In particular, for storage over a long period of time, such as over several decades, a very low temperature, for example, -180 ° C may be advantageous.
  • biobanks In recent years, several larger biomaterials banks, hereafter referred to as biobanks, have been set up in what are known as cohort studies. In such biobanks, a plurality of individual biological samples are stored for a very long time (several decades) at very low temperatures (about -180 ° C). Typically, these are more than 100,000, sometimes more than 1,000,000 individual samples stored for several decades. Such storage can also be done in particular by means of automation in so-called "high-throughput" method.
  • biobanks of large epidemiological cohort studies may, for example, be the follow-up of representative populations over decades.
  • biomaterial resources they can be used to analyze the causes of complex diseases and their early detection.
  • All such biobanks have in common that a large number of relatively small individual samples of different biomaterials are used for the single analysis use. In other words, repeated cycles of thawing and deep-freezing should be avoided.
  • these biobanks also have in common that the analysis methods are automated in the aforementioned "high-throughput" analysis methods. Therefore, the corresponding tubes for the biomaterial should be easy to automate.
  • a biobank for the storage of 25 million samples at an average storage temperature of -180 ° C in the gas phase of liquid nitrogen over a storage period of up to 30 years is currently being set up in Germany within the framework of the so-called National Cohort.
  • FIGS. 1a-1d Examples of such prior art screwed-type screw-type tubing are shown in FIGS. 1a-1d.
  • FIGS. 1a-1d Examples of such prior art screwed-type screw-type tubing are shown in FIGS. 1a-1d.
  • the space distribution in these screw tubes and screw caps from the prior art is not optimal. In other words, more space and more material is used in the prior art than would be necessary. This results in more volume and mass that must be cooled down with the biological samples and kept at a low temperature than would be necessary.
  • the construction of the known tubes sometimes also leads to artificial losses of biological sample material, which is undesirable.
  • the WO 01/90731 A2 discloses a screw cap for closing a screw tube for biomaterial.
  • the screw cap has a cylindrical screw portion with an external thread and a flange.
  • the flange has a larger outer diameter than the screw portion.
  • the lid has a recess coaxial with the longitudinal axis, which extends longitudinally through the flange and partially along the screw portion.
  • a first aspect of the present invention relates to the use of a screw cap for closing a biomaterial screw tube.
  • the screw cap can be understood as a screw and the tube as a nut.
  • the screw cap has a cylindrical screw portion having an external thread. Alternatively, this section may also be referred to as a threaded section, at least over part of its Length has a thread.
  • This cylindrical screw portion defines a longitudinal axis.
  • the screw on a flange portion which can be understood as a screw head.
  • This flange section adjoins the screw section.
  • the screw cap has a recess which extends coaxially to the longitudinal axis of the screw cap. This recess extends longitudinally through the flange portion and at least partially along the screw portion.
  • this recess has a driving inner profile.
  • This driving inner profile is suitable for opening and closing with a corresponding key. Furthermore, this driving inner profile extends axially at least partially along the screw portion.
  • the driving inner profile of the screw cap or of the rotary closure extends into a sealed cavity of the screw section, which has the external thread.
  • Such entrainment inner profile may have a relatively small diameter of less than 5 mm, preferably less than 4 mm and in particular of about 3.6 mm in comparison to the prior art, especially in the suitability for high torques for opening and Close a screw tube with the screw cap.
  • the ability to provide the driving inner profile with a relatively small diameter is advantageous, since in this way a suitable material thickness between driving profile and external thread can be realized.
  • This material thickness should in particular be large enough to ensure a suitable stability during power transmission to simultaneously driving profile and external thread.
  • the diameter of the driving profile is therefore kept as large as possible in order to keep the contact surface between wrench and screw cap as large as possible. It has been found that the above size ratios are advantageous in this respect.
  • the above-described screw cap allows a more compact and space-saving design than is possible with screw caps from the prior art. At the same time it is temperature resistant and resistant enough to be fully suitable for storage in the liquid nitrogen gas phase.
  • the screw cap according to the invention thus fulfills the objects of the invention.
  • the driving inner profile extends axially over 30% to 99% of the screw section, preferably over 50% to 90%, more preferably over 60% to 80% and in particular over about 70%. In this way, a sufficient and comparable with the prior art contact surface between driving profile and key can be created without the waste of space that have screw cap from the prior art, which do not use the screw section for the driving profile.
  • the coaxial length of the driving profile and the sealing of the tube contents are positively correlated with each other so far as maximum torque and thus the contact pressure between the screw as a screw and the tube as a mother are determined by the length of the driving profile.
  • the screw portion may have a substantially constant over the length outer diameter.
  • the flange portion corresponding to a screw head is substantially cylindrical and has a larger outer diameter (for example, 8.7 mm) than the screw portion (5.95 mm). This allows the contact surfaces of the screw tube, in particular a fferrschreibchenflansches, and the fferdeckelflansches are congruent at each rotational position, thus resulting in a maximum of sealing.
  • the flange portion is flush with the outer diameter of a fferrschreibchens.
  • the projection of the flange of the screw cap over the screw portion forms an annular contact surface with the end portion of the screw tube, for example with the flange portion of the screw tube.
  • the width of the contact surface also determines the quality of the seal between the screw tube contents and the screw tube environment.
  • the external thread is designed to close the tube in about one revolution, for example, for 0.7 to 1.3 revolutions, particularly preferably for 0.9 to 1.1 revolutions.
  • the screw cap can also be made particularly small and compact, which further contributes to size / volume and material reduction of the screw.
  • the screw cap has a longitudinal length along the longitudinal axis of a total length between 5.2 mm and 8.0 mm, more preferably between 6.2 mm and 7.0 mm, most preferably between 6.5 mm and 6.7 mm, for example, 6.6 mm. It has been found that such a dimensioning ensures a suitable compromise between the shortest possible length on the one hand and a good sealing function on the other hand.
  • the total length of the screw cap is the sum of the lengths of the screw portion and the flange portion.
  • the quotient between the length of the screw portion and the length of the flange portion is between 1.4 and 3.4, preferably between 2.1 and 2.7, more preferably between 2.3 and 2.5 and in particular about 2.4.
  • the flange portion protrudes longitudinally, typically above the screw tube. It thus contributes directly to the overall length of a system with a screw tube and a screw cap. Therefore, it is particularly preferable if the length of the flange portion is selected as small as possible.
  • the flange portion also provides a part of the sealing effect of the screw cap. Therefore, he can not be chosen arbitrarily small.
  • the above quotients are particularly suitable to realize a suitable balance between sufficient sealing effect on the one hand and the lowest possible material and space on the other hand.
  • the absolute length of the flange portion which is typically between 1.4 mm and 2.4 mm, preferably between 1.7 mm and 2.1 mm, more preferably between 1.8 mm and 2.0 mm and in particular about 1.9 mm.
  • the flan length or height is preferably sufficient, together with a Flanschrändelung to open the tube in the very rare exceptional case also manually (rule: automated opening using 'Capper Decapper' machine, if necessary by key).
  • the flange height can also ensure sufficient stability to transmit the necessary contact pressure on the sealing ring between the flange and the annular widened upper tube end.
  • the external thread may extend over 70% to 100%, preferably over 75% to 95%, more preferably over 80% to 90%, and for example over about 85% of the length of the screw portion. Again, this dimensioning has been found to be particularly advantageous in terms of sealing function on the one hand and a low material and space requirements on the other hand.
  • the screw cap comprises plastic and particularly preferably consists of plastic.
  • the screw cap in this case polypropylene and is particularly preferably made of this material. This material is particularly well suited to achieve a suitable sealing effect, even at low temperatures, even at very low temperatures. It is preferably a "medical grade" plastic or 'USP Class VI' plastic.
  • the screw cap particularly preferably has an additional sealing ring, which is preferably formed of silicone and very particularly preferably of TPE or TPV.
  • a sealing ring can further improve the seal between the screw cap according to the invention and a corresponding screw tube.
  • Flat rings are preferred in which the tube has no bevel or chamfer at the top, but preferably a flange. This type of seal is preferred because thinner.
  • a screw tube for biomaterial is designed to be closed by a screw cap used in the invention. Furthermore, the screw tube has a hollow cylinder section. This hollow cylinder section has an internal thread. This internal thread is designed to be in engagement with the external thread of the screw cap. Further, the hollow cylinder portion defines a tube axis and a tube longitudinal direction. Such a screw tube, in particular in cooperation with the aforementioned screw cap, can ensure a suitable seal, especially at low temperatures and when using liquid nitrogen, with simultaneous material and space reduction.
  • the screw tube is preferably suitable for storage at very low temperatures, for example from -180 ° or even lower temperatures.
  • the screw tube may be a nitrogen-adapted cryotube.
  • the internal thread of the hollow cylinder portion extends in the longitudinal direction of the screw tube in the vicinity of one end of the hollow cylinder portion.
  • Proximity in this context is understood to mean a distance of not more than 3 mm, preferably not more than 2 mm, more preferably not more than 1 mm and in particular not more than 0.5 mm.
  • the internal thread is thus displaced further into the vicinity of one end of the hollow cylinder section. In this way, a particularly suitable sealing can be achieved with the previously described screw cap with reduced length.
  • a flange section to adjoin an end of the hollow cylinder section.
  • Such a flange portion which may in particular have a larger area, further facilitates the sealing between the screw tube and the screw cap.
  • the screw tube preferably has a plastic and is particularly preferably made of a plastic. Particularly preferably, the screw tube to a polypropylene and very particularly preferably consists thereof.
  • the screw cap has at least one of the materials comprising the screw tube.
  • the screw cap is made of the same material or of the same materials as the screw tube.
  • screw tubes and screw caps have the same or at least similar temperature expansion properties. This can be especially important as screw caps and screw tubes are for use at very low levels Temperatures, for example, -180 ° C, can be designed. Also by the same choice of material and by the same or similar temperature properties, such as temperature coefficient of expansion, a suitable seal with low material costs and low space requirement is guaranteed.
  • the invention finds application in a storage device for receiving a plurality of screw tubes.
  • This device is designed such that the recorded screw tubes terminate flush in their longitudinal direction approximately with a flat end portion of the storage device.
  • this flush termination is such that there is a clearance between a lower end of the accommodated screw tubes and the flat end portion of the storage device that is less than 3 mm, and preferably a distance less than 2 mm, and more preferably a clearance which is smaller than 1 mm, for example, a distance of about 0.6 mm.
  • the side walls of the storage device or the "rack" compared to the prior art are reduced in height.
  • the screw tube or the tubes in height of a support surface of the storage device or the rack. This further contributes to the reduction of the space requirement in the present invention.
  • the present invention thus avoids loss of space. This leads to a reduction in space requirements and a gain in storage efficiency. Furthermore, this is accompanied by a reduction in the cooling capacity to be maintained, ie, the entire system cooling can be dimensioned smaller and designed. This can be associated with considerable financial advantages in individual cases: for example, the reduction of the primary investment costs of nitrogen storage, be it manual or automated, the reduction of equipment maintenance and repair costs and the reduction of operating and especially energy costs.
  • the present invention is also ecologically advantageous because less volume and mass must be cooled, which consumes less energy and emits less CO 2 .
  • Figures 1a-1d show errschreibchen 102 for biomaterial and corresponding screw cap 104 for closing the screw tubes from the prior art.
  • the screw cap 104 has a screw portion 106 and a projection portion 108. Further, the screw cap 104 has a sealing ring 116 made of silicone. The screw portion 106 has an external thread 110.
  • the overhang section which can be considered as a kind of screw head, has two formations, both of which serve for opening, namely a high knurling for manual opening on the outside and a recess for the opening on the inside by means of a wrench.
  • the prior art screw cap 104 has a recess 112 which extends coaxially with a longitudinal axis of the screw cap. This recess 112 is arranged in the overhang section 108 and has an entrainment inner profile, which is indicated by the lines 114.
  • Screw tube 102 is designed to be closed by the screw cap 104.
  • Screw tube 102 in this case has a hollow cylinder section 122 with an internal thread 124.
  • the internal thread 124 is typically several millimeters from the open end of the hollow cylinder 122.
  • the screw cap 4 according to the invention is according to FIGS. 2, 3 . 4, 5a . 5b and 5c to differentiate from the screw caps 104 of the prior art.
  • the screw according to the invention according to Figures 2 and 6 to distinguish from the screw tubes 102 of the prior art.
  • Screw cap 4 is suitable for closing a screw tube 2 for biomaterial.
  • the screw cap 4 has a screw portion 6.
  • This screw portion 6 is cylindrical and has an external thread 10 at one longitudinal end. Further, the screw portion 6 defines a longitudinal axis.
  • the screw cap 4 has a flange section 8, which adjoins the screw section 6.
  • the flange portion can be understood as a screw head with low knurling.
  • the flange portion 8 is formed substantially cylindrical.
  • the screw portion 6 preferably has a constant outer diameter.
  • flange portion 8 typically has a constant outer diameter that is greater than the diameter of screw portion 6 Figures 3 and 4 to see.
  • Fig. 4 a schematic sectional view of a screw cap according to the invention 4.
  • the coarsely hatched area here shows the cutting plane through the screw 4.
  • the surface 12 shows a surface of the screw 4, which is set back with respect to the cutting plane.
  • Figures 1c, 1d , and 6 are some areas opposite the cut surface are set back, finely hatched.
  • recess 112 in Fig. 1d as well as recesses 12 in Fig. 5a to 5c set back from the cut surface.
  • the recess 12 has a driving inner profile, which in Fig. 4 is indicated by lines 14.
  • This driving inner profile is designed for opening and closing with a corresponding key or suitable.
  • this entrainment inner profile, as indicated by structures 14, extends axially at least partially along the screw section 6.
  • this driving inner profile also extends at least partially along the flange portion 8.
  • this driving inner profile may also extend along the entire longitudinal direction of the recess 12. That is to say, in particular along the entire section of the recess 12 which extends along the flange section 8 and along the entire section which extends along the screw section 6.
  • a further embodiment also allows the driving inner profile not to extend along the entire flange portion 8, but to terminate at a distance from one end of the flange portion 8, in particular spaced from a surface or top 18 of the flange portion 8.
  • the recess 12 is "open” only to one side, namely to "outside”, but not to "inside”, ie not to an inner volume of the screw.
  • the cost of materials in the tubes and lids according to the invention can be reduced. Furthermore, artificial sample losses can also be avoided or reduced. These can in particular thereby can be reduced or avoided that the screw portion 6 has no recess which opens on the side opposite to the flange portion in the longitudinal direction, that has no cavity, which is arranged in use in the interior of the tube. If this is the case, as is the case with the screw caps of the prior art, liquid can accumulate in it which can be lost during opening.
  • the screw cap 4 can also be understood as a screw cap, which corresponds in its essential structure of a screw.
  • the tube 2 then corresponds to a mother.
  • a sample can guarantee.
  • FIGS. 5a-5c The respective hatched area 18 respectively designates the surface or upper side 18 of the flange section 8.
  • the hatch 12 is not hatched in these figures.
  • the entrainment inner profile may be formed by corresponding webs 22. Although in Fig. 5a six such webs are shown, it is clear to the expert that he can also 3, 4, 5, 7, 8, etc. webs use. It is also possible that instead of the webs 22 corresponding set back exceptions are formed (not shown).
  • the driving inner profile can also be done by appropriate design of the recess 12.
  • the recess 12 is formed as a polygon, here for example as an octagon 24. Again, it will be clear to the skilled person that he can arbitrarily choose from different, preferably uniform, polygons.
  • Fig. 5c shows another possible embodiment of the driving inner profile as eight-pointed star 26. Again, it will be apparent to those skilled in the art that the invention is not limited to an eight-pointed star, but that various multi-pointed stars can be used.
  • the integrated driving profile can also be designed as a so-called Torx inner profile (a star of David-like six-round) or as a so-called Torx Plus inner profile.
  • Torx inner profile a star of David-like six-round
  • Torx Plus inner profile a so-called Torx Plus inner profile.
  • These driving profiles, as well as the others described, are suitable for use in opening with a corresponding wrench outer profile.
  • the Torx profile and in particular the Torx Plus profile also allow easier placement of a wrench in an automated Capper Decapper system, in particular a good power transmission with high torque.
  • the driving inner profile can be made relatively short with a suitable power transmission, which further reduces the material and space requirements of the tube cover.
  • a high force or torque transmission and the rotary closure external thread can be made relatively short. This can lead to an increase of the inner volume of the tube at the same external height or a reduction of the outer height with the same inner volume of the tube.
  • a high power transmission with high torque but also positively affect the closing and sealing properties between the external thread 10 and an internal thread 36 of a tube and therefore allow it to be designed relatively short, for example, designed for only about one revolution. This can cause a further shortening of the total length with the same filling volume (or a higher filling volume with the same total length).
  • the driving inner profile can preferably be understood as an outwardly directed inner profile. This can be opened and closed with a Capper Decapper wrench.
  • the embodiment is also suitable for automated operation and can very particularly preferably replace a high configuration of the overhang section with inner profile, as in the prior art.
  • any non-circular design of the driving inner profile is suitable, which allows opening and closing with a corresponding key, but are preferably those that allow a good power transmission with high torque.
  • sealing ring 16 which preferably surrounds the screw portion 6.
  • this seal ring 16 is located at the portion of the screw portion 6 that does not have the external thread 10.
  • sealing ring 16 is made of silicone and particularly preferably of TPE or TPV.
  • This sealing ring 16 puts into use, as in Fig. 2 shown between the flange portion 8 of the screw cap 4 and the flange portion 38 of the screw tube 2 to seal the two elements against each other.
  • the sealing ring 16 closes (as in Fig. 4 Best seen) in the circumferential direction, which is perpendicular to the longitudinal direction, preferably flush with the flange 8 from.
  • the sealing ring in use with a tube 2 flush with an outer edge of the tube 2, for example, flush with a flange portion 38 terminates.
  • the recess 12 according to the invention and the corresponding driving inner profile extends axially at least partially along the screw portion 6. This contributes to a compact and space-saving design of the screw cap 4 according to the invention or allows them.
  • the driving inner profile extends axially over 30% to 99%, preferably over 50% to 90%, more preferably over 60% to 80%, for example over about 70% of the screw portion.
  • this parameter contributes to saving space, but on the other hand it also allows a sufficient stability of the screw cap and in particular of the screw portion. In particular, it is particularly preferred that it is ensured that the screw cap 4 can withstand very low temperatures for a very long period of time without being damaged.
  • the screw portion 6 of the screw 4 has an external thread 10. This is typically arranged at a longitudinal end of the screw section 6. It is preferred that this external thread 10 is designed approximately for one revolution, for example for 0.7 to 1.3 and particularly preferably for 0.9 to 1.1 revolutions. This configuration for only a small number of revolutions further contributes to the compactness and thus to the space savings. Furthermore, the steepness of the thread can also play a role.
  • screw cap 4 is molded from plastic and more preferably polypropylene. This ensures a lightweight construction of the screw 4. Next is a Such material so resistant that it allows the simple and compact design of the screw 4 and therefore further contributes to the compactness of the screw 4.
  • Screw tube 2 is designed to be closed by a screw cap 4 as described above.
  • Screw 2 has a hollow cylinder section 32.
  • screw 2 may also have a tapered portion 34 adjoining the hollow cylinder portion 32, which tapers with increasing distance from the hollow cylinder portion 32.
  • hollow cylinder section 32 has an internal thread 36. This internal thread is designed to be in engagement with the external thread 10 of the above-described screw cap 4.
  • the hollow cylinder section 32 of screw tubes 2 defines a tube axis as well as a tube longitudinal direction.
  • the internal thread 36 of the screw tube 2 is designed for approximately one revolution, preferably for 0.7 to 1.3 revolutions and particularly preferably for 0.9 to 1.1 revolutions.
  • the screw cap 4 is made relatively compact.
  • the thread 10 may extend only over 3 to 5 mm, for example about 3.9 mm.
  • the internal thread 36 of the hollow cylindrical portion 32 of the screw tube 2 in the longitudinal direction of the screw 2 in the vicinity of one end of the hollow cylinder portion 32 extends.
  • Proximity should in this context mean a distance of not more than 3 mm, preferably not more than 2 mm, more preferably not more than 1 mm and in particular not more than 0.5 mm.
  • a flange section 38 adjoins one end of the hollow cylinder section 32 of the screw tube 2. This can for example become a Surface enlargement, which further facilitates sealing between the screw 2 and the screw cap 4.
  • the screw tube on plastic is particularly preferably formed from a plastic or consists thereof.
  • a typical plastic that can be used for this is polypropylene.
  • the invention such as in Fig. 2 also shown a system which has on the one hand a screw 4 and on the other hand a screw 2. It is preferred that in this system screw cap 4 and screw 2 have the same materials and more preferably consist of the same materials. This may therefore be advantageous, for example, that in this case tube 2 and cover 4 have the same or similar coefficients of thermal expansion. This can be particularly important when using such tubes and samples at very low temperatures. As a result, a suitable seal between tube 2 and cover 4 can be achieved even under extreme conditions. In particular, this also makes possible a particularly compact embodiment of the tube 2 and in particular of the lid 4.
  • screw tube 2 has a tapered portion 34 at a lower end. This allows the inclusion of such tubes 2 in a suitable storage device and further serves the most residue-free sample recording when pipetting off the contents.
  • a suitable storage device is typically designed to receive a plurality of screw tubes 2.
  • the device is designed such that the recorded screw 2 close in its longitudinal direction approximately flush with a flat end portion of the storage device.
  • this flush termination is such that there is a clearance between the accommodated screw tubes 2 and the flat end portion of the storage device that is less than 3 mm, preferably a distance less than 2 mm, and more preferably a clearance that is smaller than 1 mm.
  • the distance can be about 0.6 mm.
  • Such Storage device for screw 2 further contributes to the compactness in the storage of the screw 2 and further reduces the space required for this.
  • an embodiment of the screw cap and the screw tube according to the invention is compared with a screw cap and a screw tube of the prior art.
  • the screw tube or the tube from the prior art is the so-called "0.5 ml screw cap tube” from Micronic.
  • Table 1 gives the dimensions of a prototype of the present invention in comparison with the Micronic 0.5 ml Screw Cap Tube.
  • Table 1 Table 1: Comparison of the dimensions of a prototype of the present invention with an example of the prior art.
  • ⁇ / b> All measured values for 1st and 2nd were determined with vernier calipers, 3.1. was determined by pipetting water, 3.2. about multiplication of 3.1. with factor 0.917149.
  • the height of the screw tube without the screw cap is called (1.1.).
  • the screw tubes can accommodate approximately equal volumes of fluid, even the screw tube of the prototype of the invention is 3.8 mm shorter than the prior art screw tube. This is due among other things to the more compact design of the inventive screw cap and in particular the more compact design of the external thread of the screw. As a result, less volume of the screw is required for receiving the external thread of the screw. With approximately equal working volumes, this leads to a smaller height of the screw tube.
  • the flange portion of the present prototype has a height of about 1.9 mm in the longitudinal direction, as opposed to about 7.0 mm of the prior art protrusion portion. This leads to a further reduction in the overall height of the screw cap with screw cap, when this screw cap is screwed tight, as in parameter 1.3. reproduced in the above table.
  • the maximum working volume in this case results from the pipetting determined maximum filling volume up to the lower edge of the screw portion of the screw cap divided by the factor 1.0903355. This takes into account the fact that aqueous liquids, which are typically filled into the screw tubes at room temperature, are subject to expansion when the water content freezes.
  • the screw portion 106 with external thread 110 in the conventional rotary closure of Micronic over 7.1 mm (manufacturer) or 7.25 mm (own measurement) extends (see. Figures 1b and 1d ) and the supernatant portion 108 with recess 112 and driving inner profile 114 extends over 7.2 mm (manufacturer) or 6.95 mm (own measurement). That is, these mentioned proportions each represent approximately 50% of the total height of the screw cap or the screw cap 104.
  • the flange portion 8 makes up less than 30% of the total length of the screw cap, especially since the driving internal profile in this case overlaps with the screw portion in the longitudinal direction, as by parameter 1.2.4. played.
  • This material ring is wider than in the prior art.
  • Said sealing surface, together with the torque and material of the sealing ring, determines the degree of sealing of tube contents to tube environment.
  • This design can contribute to their better sealing effect that, for example, the thread can be made shorter without the Retrohafte Abdichtrial is reduced too much, and thus can contribute to material and size savings.
  • the section of the external thread of the screw cap directed in use to the internal volume of the tube or to the internal volume of the tube is hollow in the prior art by Micronic to the inner volume of the tube.
  • This cavity is on the one hand unusable and thus wasted in the sample storage space, on the other hand at the same time an artifact source insofar as liquid tube contents (before or after possibly deep freezing), for example, by shaking into this cavity and thus lost a use.
  • the height of the screw cap according to the embodiment of the invention is only 6.6 mm, thus reduced by 54% over the prior art of Micronic.
  • the substantial reduction of this height is achieved inter alia by the overlap in the longitudinal direction between the screw portion with external thread on the one hand and the recess with driving inner profile on the other.
  • the space requirement of the tube according to the invention in the storage device is reduced by over 39% over the prior art of Micronic.
  • the considerable reduction in energy consumption (and thus also in energy costs) goes hand in hand with a considerable reduction in environmental pollution in the context of large, epidemiological cohort studies that have been going on for decades.

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
  • Mechanical Engineering (AREA)
  • Closures For Containers (AREA)
  • Prostheses (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Claims (11)

  1. Utilisation d'un bouchon vissé pour la fermeture d'un tube d'échantillon pour un matériau biologique pour le stockage à froid, dans laquelle le bouchon vissé présente une section de vis (6) cylindrique, qui présente un filetage extérieur (10), dans laquelle la section de vis (6) cylindrique définit un axe longitudinal,
    une section de bride (8), qui se raccorde à la section de vis (6), dans laquelle la section de bride (8) est sensiblement cylindrique et présente un plus grand diamètre extérieur que la section de vis (6),
    dans laquelle la longueur totale du bouchon vissé est la somme des longueurs de la section de vis et de la section de bride et le quotient entre la longueur de la section de vis et la longueur de la section de bride se situe entre 1,4 et 3,4,
    un évidement (12) coaxial à l'axe longitudinal, qui s'étend longitudinalement à travers la section de bride (8) et au moins en partie le long de la section de vis (6), dans laquelle l'évidement (12) présente un profil intérieur d'entraînement, qui convient à l'ouverture et à la fermeture avec une clé correspondante,
    dans laquelle le profil intérieur d'entraînement s'étend axialement au moins en partie le long de la section de vis (6), dans laquelle le profil intérieur d'entraînement s'étend axialement sur 30 % à 99 % de la section de vis (6).
  2. Utilisation selon la revendication 1, dans laquelle le filetage extérieur est conçu à peu près pour 0,7 à 1,3 rotation.
  3. Utilisation selon l'une quelconque des revendications précédentes, dans laquelle le bouchon vissé a dans la direction longitudinale le long de l'axe longitudinal une longueur totale entre 5,2 mm et 8,0 mm.
  4. Utilisation selon l'une quelconque des revendications précédentes, dans laquelle la longueur de la section de bride se situe entre 1,4 mm et 2,4 mm.
  5. Utilisation selon l'une quelconque des revendications précédentes, dans laquelle le filetage extérieur s'étend dans la direction longitudinale sur 70 % à 100 % de la longueur de la section de vis.
  6. Utilisation selon l'une quelconque des revendications précédentes, dans laquelle le bouchon vissé présente du plastique.
  7. Utilisation selon la revendication 6, dans laquelle le bouchon vissé présente du polypropylène.
  8. Utilisation selon l'une quelconque des revendications précédentes, dans laquelle le bouchon vissé présente en plus une bague d'étanchéité en silicone, TPE ou TPV.
  9. Utilisation selon l'une quelconque des revendications 1 à 8 avec un tube d'échantillon pour un matériau biologique, dans laquelle le tube d'échantillon présente une section cylindrique creuse avec un filetage intérieur, qui est conçu pour être en prise avec le filetage extérieur du bouchon vissé et dans laquelle la section cylindrique creuse définit un axe de tube et une direction longitudinale de tube.
  10. Utilisation selon la revendication 9, dans laquelle le bouchon vissé est formé du même matériau que le tube d'échantillon.
  11. Utilisation selon la revendication 9 ou 10, dans laquelle le tube d'échantillon présente du polypropylène.
EP13821457.2A 2012-12-21 2013-12-17 Utilisation de tubes d'échantillons et de bouchons vissés pour stockage à froid de matériel biologique Active EP2934750B1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102012025254.4A DE102012025254A1 (de) 2012-12-21 2012-12-21 Schraubröhrchen und Schraubdeckel für Biomaterial
PCT/EP2013/076872 WO2014095840A1 (fr) 2012-12-21 2013-12-17 Tubule vissable et couvercle vissé pour matériau biologique

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EP2934750A1 EP2934750A1 (fr) 2015-10-28
EP2934750B1 true EP2934750B1 (fr) 2019-08-07

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US (1) US10155608B2 (fr)
EP (1) EP2934750B1 (fr)
JP (1) JP6542126B2 (fr)
KR (1) KR102044878B1 (fr)
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DE (1) DE102012025254A1 (fr)
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US20150048085A1 (en) 2013-08-16 2015-02-19 Corning Incorporated Vessels and methods for cryopreservation
CN108430217B (zh) * 2015-11-16 2021-10-22 康宁股份有限公司 低温用瓶组件
US10638748B2 (en) 2015-12-22 2020-05-05 Corning Incorporated Break away/tear away cryopreservation vial and methods for manufacturing and using same
CN106931270A (zh) * 2017-03-21 2017-07-07 广东联塑科技实业有限公司 一种管堵螺帽结构
CN107344129B (zh) * 2017-08-31 2022-07-22 中国科学院金属研究所 一种用于空间在轨材料科学实验的通用型样品安瓿
CN111077108B (zh) * 2019-12-31 2020-08-25 中国科学院地质与地球物理研究所 一种适用于深空探测的激光样品室

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Also Published As

Publication number Publication date
DK2934750T3 (da) 2019-10-21
WO2014095840A1 (fr) 2014-06-26
DE102012025254A1 (de) 2014-06-26
KR102044878B1 (ko) 2019-11-14
AU2013361755A1 (en) 2015-08-06
US10155608B2 (en) 2018-12-18
US20160031611A1 (en) 2016-02-04
JP6542126B2 (ja) 2019-07-10
EP2934750A1 (fr) 2015-10-28
AU2013361755B2 (en) 2018-03-08
JP2016502846A (ja) 2016-02-01
KR20150096799A (ko) 2015-08-25

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