EP2877595A1 - Procédé de différenciation entre cellules vivantes et mortes - Google Patents

Procédé de différenciation entre cellules vivantes et mortes

Info

Publication number
EP2877595A1
EP2877595A1 EP13729251.2A EP13729251A EP2877595A1 EP 2877595 A1 EP2877595 A1 EP 2877595A1 EP 13729251 A EP13729251 A EP 13729251A EP 2877595 A1 EP2877595 A1 EP 2877595A1
Authority
EP
European Patent Office
Prior art keywords
dna
cells
living
amplification reaction
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP13729251.2A
Other languages
German (de)
English (en)
Other versions
EP2877595B1 (fr
Inventor
Alexandra Scholz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sartorius Stedim Biotech GmbH
Original Assignee
Sartorius Stedim Biotech GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sartorius Stedim Biotech GmbH filed Critical Sartorius Stedim Biotech GmbH
Publication of EP2877595A1 publication Critical patent/EP2877595A1/fr
Application granted granted Critical
Publication of EP2877595B1 publication Critical patent/EP2877595B1/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne un procédé de détermination quantitative de cellules vivantes et mortes dans un échantillon biologique. Le procédé de l'invention repose sur la détermination de la quantité d'ADN présente dans l'échantillon en utilisant une réaction d'amplification d'ADN qui ne dégrade pas l'intégrité de la membrane des cellules vivantes.
EP13729251.2A 2012-07-26 2013-05-27 Procédé de différenciation entre cellules vivantes et mortes Active EP2877595B1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102012014981A DE102012014981B3 (de) 2012-07-26 2012-07-26 Verfahren zur Differenzierung zwischen lebenden und toten Zellen
PCT/EP2013/001564 WO2014015925A1 (fr) 2012-07-26 2013-05-27 Procédé de différenciation entre cellules vivantes et mortes

Publications (2)

Publication Number Publication Date
EP2877595A1 true EP2877595A1 (fr) 2015-06-03
EP2877595B1 EP2877595B1 (fr) 2016-08-24

Family

ID=48628598

Family Applications (1)

Application Number Title Priority Date Filing Date
EP13729251.2A Active EP2877595B1 (fr) 2012-07-26 2013-05-27 Procédé de différenciation entre cellules vivantes et mortes

Country Status (4)

Country Link
US (1) US9738939B2 (fr)
EP (1) EP2877595B1 (fr)
DE (1) DE102012014981B3 (fr)
WO (1) WO2014015925A1 (fr)

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6410223B1 (en) 1994-11-04 2002-06-25 The United States Of America As Represented By The Secretary Of Agriculture Direct polymerase chain reaction assay, or bio-PCR
NO974299D0 (no) 1997-09-18 1997-09-18 Forskningsparken I Aas As Metode for stabil merking av mikroorganismer
US6146834A (en) 1999-09-10 2000-11-14 The United States Of America As Represented By The Secretary Of Agriculture PCR primers for detection of plant pathogenic species and subspecies of acidovorax
JP2001299399A (ja) 2000-04-14 2001-10-30 Joji Oshima 核酸定量を応用した細胞の生死判別法
WO2002052034A1 (fr) 2000-12-26 2002-07-04 Joji Oshima Methodes de bioscopie et d'amplification d'acides nucleiques
ATE400663T1 (de) * 2002-02-21 2008-07-15 Asm Scient Inc Rekombinase-polymerase-amplifikation
WO2007064313A2 (fr) 2004-11-24 2007-06-07 Invitrogen Corporation Systeme de detection et de confirmation biologiques
EP1992690B1 (fr) 2006-02-17 2014-05-21 Morinaga Milk Industry Co., Ltd. Procede et kit de detection de microorganismes
JP5295787B2 (ja) 2006-02-28 2013-09-18 モンタナ ステート ユニバーシティ 核酸に基づく分子的技法を用いて無傷細胞と膜損傷細胞とを識別するためのフェナントリジウム誘導体の使用
KR101120270B1 (ko) 2007-08-16 2012-03-06 모리나가 뉴교 가부시키가이샤 미생물 검출법 및 미생물 검출 키트
US20090123959A1 (en) 2007-10-25 2009-05-14 U.S. Environmental Protection Agency Microorganism discriminator and method
DE102008029999A1 (de) 2008-06-24 2009-12-31 Leclerque, Andreas, Dr.rer.nat. MA Neue PCR-gestützte Nachweismethode zur Unterscheidung lebender von toten Zellen
AU2010275576B2 (en) 2009-07-24 2013-02-28 Morinaga Milk Industry Co., Ltd. Method and kit for detection of microorganism
WO2011043737A1 (fr) 2009-10-05 2011-04-14 Nanyang Technological University Analyse de viabilité de protozoaires au moyen d'une amplification en chaîne par polymérase (acp)
WO2011068465A1 (fr) 2009-12-02 2011-06-09 Haiqing Gong Procede et appareil de recuperation de cellules et leur analyse
US9228240B2 (en) 2010-06-03 2016-01-05 California Institute Of Technology Methods for detecting and quantifying viable bacterial endo-spores

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO2014015925A1 *

Also Published As

Publication number Publication date
US9738939B2 (en) 2017-08-22
EP2877595B1 (fr) 2016-08-24
US20150203898A1 (en) 2015-07-23
WO2014015925A1 (fr) 2014-01-30
DE102012014981B3 (de) 2013-07-18

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