EP2781925B1 - Versiegelte afm-zelle - Google Patents

Versiegelte afm-zelle Download PDF

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Publication number
EP2781925B1
EP2781925B1 EP12849122.2A EP12849122A EP2781925B1 EP 2781925 B1 EP2781925 B1 EP 2781925B1 EP 12849122 A EP12849122 A EP 12849122A EP 2781925 B1 EP2781925 B1 EP 2781925B1
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EP
European Patent Office
Prior art keywords
liquid
main body
sealed
afm
lid part
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Not-in-force
Application number
EP12849122.2A
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English (en)
French (fr)
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EP2781925A1 (de
EP2781925A4 (de
Inventor
Takeshi Fukuma
Yukitoshi KATAGIRI
Hitoshi Asakawa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kanazawa University NUC
Original Assignee
Kanazawa University NUC
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Filing date
Publication date
Application filed by Kanazawa University NUC filed Critical Kanazawa University NUC
Publication of EP2781925A1 publication Critical patent/EP2781925A1/de
Publication of EP2781925A4 publication Critical patent/EP2781925A4/de
Application granted granted Critical
Publication of EP2781925B1 publication Critical patent/EP2781925B1/de
Not-in-force legal-status Critical Current
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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01Q—SCANNING-PROBE TECHNIQUES OR APPARATUS; APPLICATIONS OF SCANNING-PROBE TECHNIQUES, e.g. SCANNING PROBE MICROSCOPY [SPM]
    • G01Q30/00—Auxiliary means serving to assist or improve the scanning probe techniques or apparatus, e.g. display or data processing devices
    • G01Q30/08—Means for establishing or regulating a desired environmental condition within a sample chamber
    • G01Q30/12—Fluid environment
    • G01Q30/14—Liquid environment
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01Q—SCANNING-PROBE TECHNIQUES OR APPARATUS; APPLICATIONS OF SCANNING-PROBE TECHNIQUES, e.g. SCANNING PROBE MICROSCOPY [SPM]
    • G01Q10/00—Scanning or positioning arrangements, i.e. arrangements for actively controlling the movement or position of the probe
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01Q—SCANNING-PROBE TECHNIQUES OR APPARATUS; APPLICATIONS OF SCANNING-PROBE TECHNIQUES, e.g. SCANNING PROBE MICROSCOPY [SPM]
    • G01Q30/00—Auxiliary means serving to assist or improve the scanning probe techniques or apparatus, e.g. display or data processing devices
    • G01Q30/08—Means for establishing or regulating a desired environmental condition within a sample chamber
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01Q—SCANNING-PROBE TECHNIQUES OR APPARATUS; APPLICATIONS OF SCANNING-PROBE TECHNIQUES, e.g. SCANNING PROBE MICROSCOPY [SPM]
    • G01Q30/00—Auxiliary means serving to assist or improve the scanning probe techniques or apparatus, e.g. display or data processing devices
    • G01Q30/08—Means for establishing or regulating a desired environmental condition within a sample chamber
    • G01Q30/12—Fluid environment
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01Q—SCANNING-PROBE TECHNIQUES OR APPARATUS; APPLICATIONS OF SCANNING-PROBE TECHNIQUES, e.g. SCANNING PROBE MICROSCOPY [SPM]
    • G01Q30/00—Auxiliary means serving to assist or improve the scanning probe techniques or apparatus, e.g. display or data processing devices
    • G01Q30/18—Means for protecting or isolating the interior of a sample chamber from external environmental conditions or influences, e.g. vibrations or electromagnetic fields
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01Q—SCANNING-PROBE TECHNIQUES OR APPARATUS; APPLICATIONS OF SCANNING-PROBE TECHNIQUES, e.g. SCANNING PROBE MICROSCOPY [SPM]
    • G01Q60/00—Particular types of SPM [Scanning Probe Microscopy] or microscopes; Essential components thereof
    • G01Q60/24—AFM [Atomic Force Microscopy] or apparatus therefor, e.g. AFM probes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01Q—SCANNING-PROBE TECHNIQUES OR APPARATUS; APPLICATIONS OF SCANNING-PROBE TECHNIQUES, e.g. SCANNING PROBE MICROSCOPY [SPM]
    • G01Q70/00—General aspects of SPM probes, their manufacture or their related instrumentation, insofar as they are not specially adapted to a single SPM technique covered by group G01Q60/00
    • G01Q70/02—Probe holders
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y35/00—Methods or apparatus for measurement or analysis of nanostructures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01Q—SCANNING-PROBE TECHNIQUES OR APPARATUS; APPLICATIONS OF SCANNING-PROBE TECHNIQUES, e.g. SCANNING PROBE MICROSCOPY [SPM]
    • G01Q20/00—Monitoring the movement or position of the probe
    • G01Q20/02—Monitoring the movement or position of the probe by optical means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01Q—SCANNING-PROBE TECHNIQUES OR APPARATUS; APPLICATIONS OF SCANNING-PROBE TECHNIQUES, e.g. SCANNING PROBE MICROSCOPY [SPM]
    • G01Q60/00—Particular types of SPM [Scanning Probe Microscopy] or microscopes; Essential components thereof
    • G01Q60/24—AFM [Atomic Force Microscopy] or apparatus therefor, e.g. AFM probes
    • G01Q60/32—AC mode

Definitions

  • the present invention relates to a sealed AFM cell in which an observation liquid is sealed.
  • An atomic force microscope detects a force of interaction working between the surface of a sample and a probe held near the surface of the sample. Moreover, the AFM performs feedback control for the distance between the probe and the sample to keep the detected force of interaction constant. Furthermore, the AFM horizontally scans the sample while maintaining the feedback control. This allows the probe to go up and down along the surface asperities of the sample with a certain distance between the probe and the sample. The AFM can obtain a topographic image of the surface of the sample by recording the vertical movement of the probe with respect to the scanned horizontal position.
  • a cantilever having the probe at its one end is used for a force sensor for detecting a force of interaction between the probe and the sample.
  • An optical lever method for example, is widely used for detecting the displacement magnitude of this cantilever.
  • the displacement magnitude of the cantilever is detected by irradiating the back side of the cantilever with a light beam such as a laser beam and detecting light reflected off the back side with a position detection sensor such as a four-split photodiode.
  • the AFM is especially useful as a method for directly observing the shape and physical properties of the surface of a sample in a liquid.
  • an AFM for observing a sample in a liquid is referred to as liquid AFM.
  • the liquid AFM measures the surface of a sample in the state where the cantilever and the sample are immersed in an observation liquid.
  • a problem is in that evaporation of the observation liquid constantly changes the concentration of dissolved matter. Moreover, an increase in the amount of evaporation changes the solution shape of the observation liquid. Thus, a laser beam path may change. This makes it difficult to detect the accurate displacement magnitude of the cantilever.
  • Patent Literature 1 PTL 1
  • Patent Literature 2 (PTL 2)
  • a cantilever and a drive unit such as a scanner are not mechanically joined.
  • the cantilever and the drive unit such as the scanner are mechanically joined, mechanical vibration occurring in the drive unit is transmitted to the cantilever. This makes it difficult to accurately detect the displacement magnitude of the cantilever.
  • the cantilever and the scanner are mechanically joined with the rubber O ring to seal the AFM cell.
  • vibration of the scanner is transmitted to the cantilever via the O ring.
  • a cantilever is oscillated at a minute amplitude to detect a force of interaction between a probe and a sample as a change in frequency, phase, amplitude, or others of the oscillation of the cantilever.
  • an acoustic excitation method using acoustic waves generated in a piezoelectric vibrator is widely used as an excitation method for oscillating the cantilever.
  • the present invention provides a sealed AFM cell which does not decrease measurement accuracy or limit usable observation liquids.
  • An aspect of a sealed atomic force microscope (AFM) cell is a sealed AFM cell for an AFM which measures a sample immersed in an observation liquid according to claim 1.
  • the sealed AFM cell includes: a cantilever including a probe; a sample holder for fixing the sample; a scanner which is a drive unit which moves the sample holder; a lid part which holds the cantilever so as to position the probe near a measurement surface of the sample; and a main body part which is a component for holding the scanner and positioned opposite the lid part with the sample in between, in which the lid part and the main body part are joined via a sealing liquid to seal the observation liquid inside a space formed by the lid part, the main body part, and the sealing liquid, the sealing liquid being different from the observation liquid and not in contact with the observation liquid.
  • This configuration allows the sealed AFM cell to seal the observation liquid inside the space formed by the lid part, main body part, and the sealing liquid. Therefore, vibration of the scanner or others can be prevented from being transmitted to the cantilever while suppressing evaporation of the observation liquid. Moreover, the sealing liquid and the observation liquid are not in contact. Therefore, usable observation liquids are not limited to particular liquids.
  • At least one of the lid part and the main body part has a groove formed by two components having water repellency or oil repellency and the lid part or the main body part, and the groove holds the sealing liquid.
  • the sealed AFM cell has a groove formed by the component having water repellency or oil repellency and the lid part or the main body part. Therefore, the sealed AFM cell can hold the sealing liquid more stably.
  • At least one of the lid part and the main body part has a wall protruding from one of the lid part and the main body part toward the other to surround the sample holder, and an end portion of the wall holds the sealing liquid.
  • the end portion of the wall forms a groove together with two components having water repellency or oil repellency, and the groove holds the sealing liquid.
  • the wall of the sealed AFM cell can hold the sealing liquid more stably.
  • the sealed AFM cell may further include a tube for replenishing the sealing liquid.
  • the sealed AFM cell can seal the inside without damaging sealing properties for many hours.
  • the sealing liquid may be water, ionic liquid, fluorine-based inert liquid, or silicone oil.
  • the present invention can be not only achieved as such sealed AFM cell, but also achieved as an AFM having part or all of the functions of the sealed AFM cell.
  • the present invention can provide a sealed AFM cell which does not decrease measurement accuracy or limit usable observation liquids.
  • FIG. 1 illustrates a configuration of an atmosphere open AFM cell 900 according to a related art of the present invention.
  • FIG. 1 illustrates, in the atmosphere open AFM cell 900, an observation liquid is not sealed inside the AFM cell.
  • the atmosphere open AFM cell 900 includes a detection unit 910 and a drive unit 930. As described above, the detection unit 910 and the drive unit 930 are not mechanically joined. A sample 140 immersed in an observation liquid 150 is placed between the detection unit 910 and the drive unit 930.
  • the detection unit 910 includes a piezoelectric vibrator 112, an optical window 114, a cantilever 120, and a lid part 122.
  • the piezoelectric vibrator 112 includes a piezoelectric element which vibrates when a voltage is applied.
  • the piezoelectric vibrator 112 has a configuration, for example, in which a piezo-ceramic element is joined to a metal-made vibrator plate.
  • the atmosphere open AFM cell 900 detects a force of interaction between a probe and a sample by the approach called dynamic mode AFM.
  • the piezoelectric vibrator 112 is an excitation unit for oscillating the cantilever.
  • the optical window 114 is a transparent window provided in the lid part.
  • the optical window 114 is made of a material (such as a glass) which transmits a laser beam.
  • the atmosphere open AFM cell 900 detects the displacement magnitude of the cantilever by the optical lever method.
  • a light beam (such as a laser beam) emitted from a light beam element 116 transmits through the optical window 114, and is reflected off the back side of the cantilever.
  • the reflected light transmits through the optical window 114, and is detected by a position detection sensor 118 including a photodiode.
  • the cantilever 120 is a component having a cantilever structure.
  • the cantilever 120 has one end at which a probe is provided and the other end which is mechanically joined to the lid part.
  • the cantilever 120 is displaced depending on the force of interaction working between the sample 140 and the probe.
  • the lid part 122 holds the cantilever 120 so as to position the probe near the measurement surface, i.e., the surface of the sample 140.
  • the drive unit 930 includes a sample holder 132 and a scanner 134.
  • the sample holder 132 fixes the sample 140. Specifically, the sample 140 is fixed to the sample holder 132 such that the measurement surface is directed upward.
  • the scanner 134 is a drive unit which moves the sample holder 132 in a horizontal plane. Moreover, the scanner 134 controls the distance between the probe and the sample by vertically moving the sample holder 132.
  • the observation liquid 150 evaporates during the measurement. As a result, measurement accuracy declines.
  • FIG. 2A illustrates a configuration of a sealed AFM cell 900A according to another related art of the present invention. It should be noted that the same reference sings are given to structural elements similar to those illustrated in FIG. 1 , and the detailed explanation will be omitted.
  • the sealed AFM cell 900A includes a rubber O ring 160 between the lid part 122 and the sample holder 132. Provision of the O ring 160 seals the observation liquid 150 inside the space formed by the lid part 122, the O ring 160, and the sample holder 132.
  • FIG. 2B illustrates an example of the state in which the scanner 134 applies a lateral force to the sealed AFM cell 900A.
  • the observation liquid 150 is sealed with the O ring. Therefore, the accuracy of displacement measurement for the cantilever 120 declines.
  • a sealed AFM cell according to the present invention can solve these problems. This will be described in detail below.
  • FIG. 3 illustrates a configuration of a sealed AFM cell 100 according to Embodiment 1 not being part of the present claimed invention. It should be noted that the same reference signs are given to structural elements similar to those of the atmosphere open AFM cell 900 illustrated in FIG. 1 , and the detailed explanation will be omitted.
  • the sealed AFM cell 100 includes a detection unit 110 and a drive unit 130. Unlike the atmosphere open AFM cell 900, the detection unit 110 and the drive unit 130 are joined via a sealing liquid 170. That is, the sealed AFM cell 100 seals the observation liquid 150 inside the space formed by the lid part 122, a main body part 136, and the sealing liquid 170.
  • the detection unit 110 includes the piezoelectric vibrator 112, the optical window 114, the cantilever 120, and the lid part 122.
  • the drive unit 130 includes the sample holder 132, the scanner 134, and the main body part 136.
  • the main body part 136 holds the scanner 134.
  • the main body part 136 is opposite the lid part 122 with the sample 140 on the sample holder 132 in between. More specifically, as FIG. 3 illustrates, the main body part has a depression shape, and the lid part 122 has a flat shape which allows to lid the main body part 136 in the depression shape.
  • the shapes of the lid part 122 and the main body part 136 are not limited to the above shapes. However, the lid part 122 and the main body part 136 take any shapes which allow a sealed space to be formed when the lid part 122 and the main body part 136 are joined via the sealing liquid. For instance, the lid part 122 may have a downward depression shape, and the main body part 136 may have a flat shape. Moreover, the lid part 122 may have a downward depression shape, and the main body part 136 may have a depression shape.
  • At least one of the lid part 122 and the main body part 136 has a wall protruding from one of the lid part 122 and the main body part 136 toward the other to surround the sample holder 132.
  • the end portion of the protruding wall holds the sealing liquid 170 which is different from the observation liquid 150.
  • the structure of the lid part 122 and the main body part 136 does not allow the observation liquid 150 and the sealing liquid 170 to be in contact with each other. Therefore, any liquids suitable for the respective applications of the observation liquid 150 and the sealing liquid 170 can be used.
  • Liquids usable as the sealing liquid 170 are, for example, water, ionic liquid, fluorine-based inert liquid, and silicone oil.
  • the lid part 122 and the main body part 136 are joined via the sealing liquid 170.
  • the sealed AFM cell 100 seals the observation liquid 150 inside the space formed by the lid part 122, the main body part 136, and the sealing liquid 170.
  • a sealed space where evaporation of the observation liquid 150 is suppressed is formed by joining the lid part 122 and the main body part 136 via the sealing liquid 170. Moreover, the lid part 122 and the main body part 136 are joined only by the sealing liquid 170. Therefore, the lid part 122 and the main body part 136 are mechanically separated. Therefore, vibration on the side where drive unit 130 is located, generated by the scanner 134 or others can be prevented from being transmitted to the cantilever 120.
  • the shape of the sealing liquid 170 freely changes independently of mechanical components.
  • the sealing liquid 170 has fewer limitations for the thickness of the sample 140 and an observation area, than the related art using, for example, an O ring.
  • observation liquid 150 and the sealing liquid 170 are not in direct contact. Therefore, it is possible to select various liquids for the observation liquid 150 and the sealing liquid 170 to be matched with the application of each liquid.
  • FIG. 4A is a conceptual diagram illustrating transverse waves propagating in a medium.
  • the direction in which the medium vibrates is perpendicular to the direction in which the waves travel.
  • FIG. 4B is a conceptual diagram illustrating longitudinal waves (compressional waves) propagating in a medium.
  • the direction in which the medium vibrates is parallel with the direction in which the waves travel.
  • Vibration generated by the scanner 134 or others includes both of the transverse waves and longitudinal waves.
  • the transverse waves do not propagate in a liquid medium. Therefore, the transverse waves in the mechanical vibration generated by the scanner 134 or others do not propagate to the cantilever 120 via the sealing liquid 170.
  • the longitudinal waves propagate in the liquid medium.
  • acoustic impedance is significantly different between a solid medium and a liquid medium. Therefore, transmission of the longitudinal waves on the interface therebetween is remarkably low. Therefore, the longitudinal waves in the mechanical vibration generated by the scanner 134 or others can be prevented from propagating to the cantilever 120.
  • the basic configuration of the sealed AFM cell 100 according to Embodiment 1 was described above.
  • the following describes a configuration of a sealed AFM cell capable of more stably joining the detection unit 110 and the drive unit 130 via the sealing liquid 170.
  • FIG. 5 illustrates the configuration of a sealed AFM cell 100A according to Embodiment 2 being part of the present claimed invention.
  • a groove 171A is formed in the end portion of the wall of a main body part 136A, and a groove 171B is formed in a portion opposite the end portion of the wall, in the back side of a lid part 122A.
  • the groove 171A formed in the end portion of the wall of the main body part 136A is formed by components 172A having water repellency and the end portion of the wall of the main body part 136A.
  • the groove 171B formed in the portion opposite the end portion of the wall, in the back side of the lid part 122A is formed by components 172B having water repellency and the lid part 122A.
  • the grooves 171A and 171B are filled with the sealing liquid 170 through which the detection unit 110 and the drive unit 130 are joined.
  • the end portion of the wall forms the groove 171A together with the two components 172A having water repellency.
  • the groove 171A holds the sealing liquid 170. Silicone rubber or others may be, for example, used for the components 172A having water repellency.
  • the lid part 122A is, for example, a disk-shaped component.
  • the main body part 136A is a cylinder-shaped component having a disk-shaped bottom having a diameter the same as that of the lid part 122A and a wall protruding toward the lid part 122A to surround the sample holder 132, in the circumference of the bottom.
  • the two components 172A in loop shapes and of different diameters having water repellency are formed in the end portion of the wall of the main body part 136A.
  • the groove 171A is formed by the two components 172A and the end portion of the wall of the main body part 136A.
  • the two components 172B in loop shapes and of different diameters having water repellency are formed at positions opposite the components 172A formed in the main body part 136A, in the back side of the lid part 122A.
  • the groove 171B is formed by the two components 172B and the lid part 122A.
  • the sealing liquid 170 is held in the grooves 171A and 171B respectively formed in the main body part 136A and the lid part 122A.
  • the components 172A and the components 172B may be components having oil repellency but not water repellency.
  • the water repellency and oil repellency are the properties of the components 172A and 172B when a contact angle represented by a droplet of the sealing liquid 170 represents at least 10 degrees on the surfaces of the components 172A and 172B.
  • the sealed AFM cell 100A may include a tube 174 for replenishing the grooves 171A and 171B with the sealing liquid 170.
  • the tube 174 is provided in the lid part 122A.
  • the tube 174 may be provided in the main body part 136A.
  • FIG. 6 illustrates the comparison of changes in volume of observation liquids in two kinds of AFM cells.
  • the vertical axis represents the volumes of the observation liquids 150 normalized with respect to the volumes of the observation liquids 150 when the observation is started.
  • the horizontal axis represents measuring time (minute). It should be noted that experiments were performed five times to ensure the accuracy of data. Each point represents the average value of the five times. Moreover, the bars extending above and below each point are error bars representing the range of standard deviation.
  • the sealed AFM cell 100A 200 microliter ( ⁇ l) of phosphate buffered saline (PBS), which is the observation liquid 150 were dropped on the sample holder 132 and sealed using pure water as the sealing liquid 170. Then, a room temperature was maintained at 25 degrees centigrade, and a change in volume of the observation liquid 150 was observed.
  • PBS phosphate buffered saline
  • FIG. 6 illustrates, in the atmosphere open AFM cell, the volume of the observation liquid 150 decreases with the lapse of the measuring time, and around 20% evaporated for 90 minutes. Meanwhile, in the sealed AFM cell 100A, no significant change was seen in the volume of the observation liquid 150 even after 90 minutes has passed.
  • the displacement of the cantilever 120 could be detected for many hours by using the sealed AFM cell 100A.
  • the sealed AFM cell 100A can sufficiently suppress evaporation of the observation liquid 150. Moreover, mechanical separation of the cantilever 120 and a drive unit such as the scanner 134 can prevent the vibration of the drive unit from being transmitted to the cantilever 120.
  • each structural element is illustrated linearly.
  • the present invention also includes round corners and curved sides formed for manufacturing reasons.
  • the main body part 136 has a wall.
  • at least one of the lid part 122 and the main body part 136 may have a wall.
  • a lid part 122B may have a wall protruding toward a main body part 136B.
  • the sealed AFM cell according to Embodiment 1 or 2 does not necessarily have a wall.
  • at least one of the lid part and the main body part may have a groove formed with a component having water repellency or oil repellency, and the groove may hold a sealing liquid inside.
  • the sealed AFM cell according to Embodiment 1 or 2 and at least part of the functions of a modification thereof may be combined.
  • the present invention also includes various modifications obtained by making changes, which those skilled in the art would conceive, to the embodiment unless the modifications depart from the scope of the present invention as defined in the appended claims.
  • the numerals used above are exemplified to specifically explain the entirety of the present invention.
  • the present invention is not limited to the exemplified numerals.
  • the connections between structural elements are exemplified to specifically explain the present invention, and connections for achieving the functions of the present invention are not limited to the exemplified connections.
  • the configuration of the above sealed AFM cell is exemplified to specifically explain the present invention.
  • the sealed AFM cell according to the present invention does not necessarily have all of the structural elements of the above configuration. That is, the sealed AFM cell according to the present invention may have minimum structural elements which can achieve the advantages of the present invention.
  • the present invention is applicable to a sealed AFM cell for sealing an observation liquid.

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Radiology & Medical Imaging (AREA)
  • Electromagnetism (AREA)
  • Length Measuring Devices With Unspecified Measuring Means (AREA)
  • Microscoopes, Condenser (AREA)

Claims (3)

  1. Abgedichtete Rasterkraftmikroskop-(AFM)-Zelle für ein AFM, die eine Probe (140) misst, welche in eine Beobachtungsflüssigkeit (150) eingetaucht ist, wobei die geschlossene AFM-Zelle umfasst:
    einen Ausleger (120), der eine Sonde aufweist;
    einen Probenhalter (132) zum Befestigen der Probe (140);
    einen Scanner (134), der eine Antriebsvorrichtung ist, die dafür ausgelegt ist, den Probenhalter (132) zu bewegen;
    einen Deckelteil (122; 122A; 122B), der den Ausleger (120) hält, um so die Sonde in der Nähe der Messfläche der Probe (140) zu positionieren;
    einen Hauptkörperteil (136; 136A), der eine Körperkomponente zum Halten des Scanners (134) ist und gegenüber dem Deckelteil (122; 122A; 122B) mit der Probe (140) dazwischen positioniert ist;
    zwei Komponenten (172, 172B), die bei mindestens einem von dem Deckelteil (122; 122A; 122B) und dem Hauptkörperteil (136; 136A) vorgesehen sind, wobei die zwei Komponenten (172A, 172B) wasserabweisende oder ölabweisende Eigenschaften haben; und
    eine Dichtungsflüssigkeit (170), die durch die zwei Komponenten (172A, 172B) gehalten wird, wobei die Dichtungsflüssigkeit (170) sich von der Beobachtungsflüssigkeit unterscheidet und nicht mit der Beobachtungsflüssigkeit in Kontakt steht, und wobei die wasserabweisenden oder ölabweisenden Eigenschaften der zwei Komponenten (172A, 172B) gegenüber der Dichtungsflüssigkeit (170) ausreichend sind, sodass ein Kontaktwinkel, der durch ein Tröpfchen der Dichtungsflüssigkeit (170) repräsentiert wird, mindestens 10 Grad auf den Oberflächen der zwei Komponenten (172A, 172B) repräsentiert;
    wobei der Deckelteil (122; 122A; 122B) und der Hauptkörperteil (136; 136A) miteinander über die Dichtungsflüssigkeit verbunden sind,
    wobei die Beobachtungsflüssigkeit (150) in einem Raum abgedichtet ist, der durch den Deckelteil (122; 122A; 122B), den Hauptkörperteil (136; 136A) und die Dichtungsflüssigkeit (170) gebildet wird,
    wobei mindestens eines von dem Deckelteil (122; 122A; 122B) und dem Hauptkörperteil (136; 136A) eine Wand hat, die von einem von dem Deckelteil (122; 122A; 122B) und dem Hauptkörperteil (136; 136A) zum anderen von dem Deckelteil (122; 122A; 122B) und dem Hauptkörperteil (136; 136A) vorragt, um den Probenhalter (132) zu umschließen,
    wobei die zwei Komponenten (172A, 172B) auf einem Endabschnitt der Wand vorgesehen sind,
    wobei mindestens eines von dem Deckelteil (122; 122A; 122B) und dem Hauptkörperteil (136; 136A) eine Nut hat,
    wobei die Nut durch die zwei Komponenten (172A, 172B) und durch das mindestens eine von dem Deckelteil (122; 122A; 122B) und dem Hauptkörperteil (136; 136A) gebildet wird, auf der sich die Dichtungskomponente befindet, und
    wobei die Nut die Dichtungsflüssigkeit (170) zurückhält.
  2. Abgedichtete AFM-Zelle nach Anspruch 1, die ferner ein Rohr zum Nachfüllen der Dichtungsflüssigkeit (170) umfasst.
  3. Abgedichtete AFM-Zelle nach einem der Ansprüche 1 oder 2, wobei die Dichtungsflüssigkeit (170) Wasser, ionische Flüssigkeit, inerte Flüssigkeit auf Fluorbasis oder Silikonöl ist.
EP12849122.2A 2011-11-15 2012-11-15 Versiegelte afm-zelle Not-in-force EP2781925B1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2011249451 2011-11-15
PCT/JP2012/007326 WO2013073186A1 (ja) 2011-11-15 2012-11-15 密閉型ａｆｍセル

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Publication Number Publication Date
EP2781925A1 EP2781925A1 (de) 2014-09-24
EP2781925A4 EP2781925A4 (de) 2015-11-18
EP2781925B1 true EP2781925B1 (de) 2019-09-18

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JP2019200179A (ja) * 2018-05-18 2019-11-21 株式会社島津製作所 試料容器用装着部材及び試料容器の密閉方法
CN113109593B (zh) * 2021-04-20 2021-11-02 西南交通大学 应用于扫描探针显微镜的摆动式多模式组合探针测试装置

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EP2781925A1 (de) 2014-09-24
US20140289910A1 (en) 2014-09-25
JPWO2013073186A1 (ja) 2015-04-02
JP5761675B2 (ja) 2015-08-12
EP2781925A4 (de) 2015-11-18
US9110093B2 (en) 2015-08-18
WO2013073186A1 (ja) 2013-05-23

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