EP2480922A1 - Microscope - Google Patents

Microscope

Info

Publication number
EP2480922A1
EP2480922A1 EP10754926A EP10754926A EP2480922A1 EP 2480922 A1 EP2480922 A1 EP 2480922A1 EP 10754926 A EP10754926 A EP 10754926A EP 10754926 A EP10754926 A EP 10754926A EP 2480922 A1 EP2480922 A1 EP 2480922A1
Authority
EP
European Patent Office
Prior art keywords
detection
illumination
beam path
axis
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP10754926A
Other languages
German (de)
English (en)
Inventor
Helmut Lippert
Matthias Wald
Christopher Power
Robert Hauschild
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Carl Zeiss Microscopy GmbH
Original Assignee
Carl Zeiss MicroImaging GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Carl Zeiss MicroImaging GmbH filed Critical Carl Zeiss MicroImaging GmbH
Priority to EP16193117.5A priority Critical patent/EP3173838B1/fr
Publication of EP2480922A1 publication Critical patent/EP2480922A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/24—Base structure
    • G02B21/241—Devices for focusing
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/06—Means for illuminating specimens

Definitions

  • the sample space is adversely affected, which can have a negative effect in particular in a horizontal detection beam path. In the worst case, this also includes the removal and emptying of the sample chamber. Subsequently, a re-focusing is usually necessary. In addition, the sample is unnecessarily heated or cooled.
  • switching mirror instead of a switching mirror, it is also possible to use other switching elements, for example acousto-optical or electro-optical switching elements, which can work both on a reflective and equivalent basis on a transmissive basis. It is important compared to the integration time of the area detector - this is usually no more than 20 ms, otherwise vibrations can be negatively noticeable - short switching time, which must be such that the sample area within the integration time at least once from each Direction is illuminated. Multiple scans from any direction are also conceivable if the switchover time is small enough, for example less than 5 ms.
  • the illumination zoom element 21 and the detection zoom element 6 are connected via a control circuit for setting the Adjusting the illumination zoom element 21 in dependence on the detection image field size predetermined by the detection zoom element 6.
  • the illumination-side image field size is automatically adapted to this change when the detection image field size changed by an observer, for example, by hand.
  • the observer can therefore dispense with an illumination-side adaptation of the illumination zoom element 21; this is done by the control circuit, so that the optimum numerical aperture for the illumination is always set.

Landscapes

  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Microscoopes, Condenser (AREA)

Abstract

L'invention concerne un microscope comportant un dispositif d'éclairage (19) permettant de produire un feuillet lumineux pour l'éclairage d'une zone d'échantillon (P), s'étendant de façon approximativement plane en direction d'un axe d'éclairage (X) d'une trajectoire de faisceau d'éclairage et en direction d'un axe transversal (Y) perpendiculaire à l'axe d'éclairage (X), et comportant également un dispositif de détection (1) permettant de détecter de la lumière émise depuis la zone d'échantillon (P) le long d'un axe de détection (Z) d'une trajectoire de faisceau de détection. L'axe d'éclairage (X) et l'axe de détection (Z), et l'axe transversal (Y) et l'axe de détection (Z) forment un angle non nul, et le dispositif de détection comporte par ailleurs un objectif de détection dans la trajectoire de faisceau de détection. Dans un tel microscope, le dispositif de détection (1) comporte également un élément de détection optique disposé spatialement séparé d'une lentille frontale de l'objectif de détection (2) et pouvant être réglé indépendamment de la lentille frontale, l'élément de détection optique permettant de faire varier en continu la taille d'un champ de détection et/ou de décaler en continu un plan focal de détection dans la zone d'échantillon (P).
EP10754926A 2009-09-24 2010-09-17 Microscope Withdrawn EP2480922A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP16193117.5A EP3173838B1 (fr) 2009-09-24 2010-09-17 Microscope à feuille de lumière

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102009044983A DE102009044983A1 (de) 2009-09-24 2009-09-24 Mikroskop
PCT/EP2010/063665 WO2011036094A1 (fr) 2009-09-24 2010-09-17 Microscope

Related Child Applications (1)

Application Number Title Priority Date Filing Date
EP16193117.5A Division EP3173838B1 (fr) 2009-09-24 2010-09-17 Microscope à feuille de lumière

Publications (1)

Publication Number Publication Date
EP2480922A1 true EP2480922A1 (fr) 2012-08-01

Family

ID=43064466

Family Applications (2)

Application Number Title Priority Date Filing Date
EP16193117.5A Active EP3173838B1 (fr) 2009-09-24 2010-09-17 Microscope à feuille de lumière
EP10754926A Withdrawn EP2480922A1 (fr) 2009-09-24 2010-09-17 Microscope

Family Applications Before (1)

Application Number Title Priority Date Filing Date
EP16193117.5A Active EP3173838B1 (fr) 2009-09-24 2010-09-17 Microscope à feuille de lumière

Country Status (6)

Country Link
US (2) US9110301B2 (fr)
EP (2) EP3173838B1 (fr)
JP (1) JP5635104B2 (fr)
DE (1) DE102009044983A1 (fr)
ES (1) ES2923001T3 (fr)
WO (1) WO2011036094A1 (fr)

Families Citing this family (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5926936B2 (ja) * 2011-11-28 2016-05-25 オリンパス株式会社 顕微鏡
US9404869B2 (en) 2012-10-09 2016-08-02 Howard Hughes Medical Institute Multiview light-sheet microscopy
DE102012020241A1 (de) * 2012-10-12 2014-04-17 Carl Zeiss Microscopy Gmbh Bildaufnahmevorrichtung und Verfahren zur Aufnahme einer Bildersequenz
DE102012020242A1 (de) * 2012-10-12 2014-04-17 Carl Zeiss Microscopy Gmbh Bildaufnahmevorrichtung und Verfahren zur Aufnahme einer Bildersequenz
TR201811449T4 (tr) * 2012-11-07 2018-09-21 Artec Europe S A R L Üç boyutlu nesnelerin doğrusal boyutlarını gözetlemek için yöntem.
DE102013208926A1 (de) * 2013-05-14 2014-11-20 Carl Zeiss Microscopy Gmbh Verfahren zur 3D-hochauflösenden Lokalisierungsmikroskopie
US10539772B2 (en) 2013-10-09 2020-01-21 Howard Hughes Medical Institute Multiview light-sheet microscopy
GB201318919D0 (en) 2013-10-25 2013-12-11 Isis Innovation Compact microscope
DE102013019951B4 (de) * 2013-11-27 2023-06-15 Carl Zeiss Microscopy Gmbh Lichtmikroskop und Mikroskopieverfahren zum Untersuchen mehrerer mikroskopischer Objekte
JP2017517761A (ja) * 2014-05-30 2017-06-29 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー 大きなインタクトな組織試料を撮像するための方法及び装置
US10181190B2 (en) 2014-11-04 2019-01-15 Olympus Corporation Microscope and microscope image acquisition method
JP2016090766A (ja) * 2014-11-04 2016-05-23 オリンパス株式会社 顕微鏡
FR3031196B1 (fr) * 2014-12-29 2017-01-13 Karla Balaa Dispositif pour realiser de la microscopie a feuille de lumiere
JP6635052B2 (ja) * 2015-02-05 2020-01-22 株式会社ニコン 構造化照明顕微鏡、及び観察方法
DE102015203844A1 (de) 2015-03-04 2016-09-08 Carl Zeiss Meditec Ag Optiksystem und Operationsmikroskop
DE202016009206U1 (de) 2015-04-13 2024-03-11 Leica Microsystems CMS GmbH Vorrichtung zum Untersuchen einer Probe
GB201507021D0 (en) 2015-04-24 2015-06-10 Isis Innovation Compact microscope
WO2016178856A1 (fr) * 2015-05-01 2016-11-10 The Board Of Regents Of The University Of Texas System Feuilles de lumière uniformes et évolutives générées par focalisation étendue
DE102015109645B3 (de) * 2015-06-17 2016-09-08 Carl Zeiss Microscopy Gmbh Verfahren zur Multiliniendetektion
US10802262B2 (en) 2015-10-29 2020-10-13 The Board Of Trustees Of The Leland Stanford Junior University Methods and systems for imaging a biological sample
US9964754B2 (en) * 2015-12-11 2018-05-08 Finger Lakes Instrumentation, Llc Method, apparatus, and computer-readable medium for switching optical paths
LU92925B1 (de) 2015-12-23 2017-09-25 Leica Microsystems Verfahren zum Untersuchen einer Probe mittels Lichtblatt-Mikroskopie und Lichtblatt-Mikroskop
ES2630733B1 (es) * 2016-01-21 2018-07-04 4D-Nature Imaging Consulting, S.L Dispositivo automático de cambio de objetivo para microscopio de haz láser plano
EP3208604B1 (fr) * 2016-02-22 2019-07-10 F. Hoffmann-La Roche AG Système optique pour analyse de micropuits
DE102016103182B4 (de) * 2016-02-23 2018-04-12 Leica Microsystems Cms Gmbh Lichtblattmikroskop und Verfahren zur lichtmikroskopischen Abbildung einer Probe
US10509215B2 (en) * 2016-03-14 2019-12-17 Olympus Corporation Light-field microscope
LU93022B1 (de) 2016-04-08 2017-11-08 Leica Microsystems Verfahren und Mikroskop zum Untersuchen einer Probe
WO2017180680A1 (fr) 2016-04-12 2017-10-19 The Board Of Regents Of The University Of Texas System Microscope à feuille de lumière avec acquisition d'images 3d parallélisées
DE102016108384B3 (de) * 2016-05-04 2017-11-09 Leica Microsystems Cms Gmbh Vorrichtung und Verfahren zur lichtblattartigen Beleuchtung einer Probe
AU2017281533B2 (en) 2016-06-24 2019-06-27 Howard Hughes Medical Institute Automated adjustment of light sheet geometry in a microscope
LU93143B1 (de) 2016-07-06 2018-03-05 Leica Microsystems Verfahren zum Untersuchen einer Probe sowie Vorrichtung zum Ausführen eines solchen Verfahrens
DE102017107915A1 (de) * 2016-07-18 2018-01-18 Carl Zeiss Meditec Ag System zur Augentherapie mittels Gewebebearbeitung durch nichtlineare Wechselwirkung
WO2018033581A1 (fr) * 2016-08-15 2018-02-22 Leica Microsystems Cms Gmbh Microscope à feuille de lumière
US11428916B2 (en) * 2016-08-15 2022-08-30 Leica Microsystems Cms Gmbh Light sheet microscope
DE102017119169B4 (de) 2016-08-22 2023-07-27 Leica Microsystems Cms Gmbh Verfahren und Vorrichtung zur SPIM-Untersuchung einer Probe
EP3538941A4 (fr) 2016-11-10 2020-06-17 The Trustees of Columbia University in the City of New York Procédés d'imagerie rapide de grands échantillons à haute résolution
US10469758B2 (en) 2016-12-06 2019-11-05 Microsoft Technology Licensing, Llc Structured light 3D sensors with variable focal length lenses and illuminators
US10554881B2 (en) 2016-12-06 2020-02-04 Microsoft Technology Licensing, Llc Passive and active stereo vision 3D sensors with variable focal length lenses
EP3332695B1 (fr) 2016-12-09 2021-05-26 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Dispositif de mesure
CN114779459A (zh) 2017-09-29 2022-07-22 徕卡生物系统成像股份有限公司 实时自动聚焦扫描
WO2019092132A1 (fr) * 2017-11-10 2019-05-16 Viventis Microscopy Sàrl Microscope d'imagerie d'un échantillon, et porte-échantillon pour ledit microscope
DE102018210603A1 (de) * 2018-06-28 2020-01-02 Carl Zeiss Microscopy Gmbh Verfahren zum Erzeugen eines Übersichtsbilds unter Verwendung eines hochaperturigen Objektivs
DE102018216038A1 (de) 2018-09-20 2020-03-26 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zur Erfassung eines Bildes eines Objekts mit einem Scanmikroskop
WO2021146280A1 (fr) 2020-01-14 2021-07-22 Intelligent Imaging Innovations, Inc. Illuminateur en treillis cylindrique simplifié de feuillets lumineux pour microscope à feuillets lumineux

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH05290317A (ja) * 1992-04-13 1993-11-05 Mitsubishi Electric Corp 磁気ヘッドおよびその製造方法
JP3670745B2 (ja) 1996-02-20 2005-07-13 オリンパス株式会社 共焦点顕微鏡
DE19654211C2 (de) 1996-12-24 2000-07-06 Leica Microsystems Konfokales Mikroskop
DE19822255C2 (de) 1998-05-18 2001-07-05 Zeiss Carl Jena Gmbh Auflicht-Beleuchtungsanordnung für ein Stereomikroskop
DE10222041B4 (de) * 2002-05-10 2006-01-26 Leica Microsystems (Schweiz) Ag Afokales Zoomsystem zur Verwendung in Mikroskopen
DE10225193B4 (de) 2002-06-06 2004-08-12 Leica Microsystems (Schweiz) Ag Verfahren zur Kalibrierung der Vergrößerung eines Mikroskops sowie kalibrierbares Mikroskop
DE10257423A1 (de) * 2002-12-09 2004-06-24 Europäisches Laboratorium für Molekularbiologie (EMBL) Mikroskop
DE10359733A1 (de) * 2003-12-19 2005-07-14 Carl Zeiss Jena Gmbh Afokales Zoomsystem
WO2005095993A1 (fr) * 2004-03-31 2005-10-13 The Tokyo Electric Power Company, Incorporated Systeme de mesure de fluide, procede de mesure de fluide et programme informatique
DE102004034990A1 (de) 2004-07-16 2006-02-02 Carl Zeiss Jena Gmbh Zoomoptik für ein Lichtrastermikroskop mit linienförmiger Abtastung und Verwendung
JP4928836B2 (ja) 2006-05-30 2012-05-09 日本分光株式会社 顕微装置
DE102007011305A1 (de) 2007-03-06 2008-09-11 Leica Microsystems Cms Gmbh Vorrichtung und Verfahren zur Strahljustage in einem optischen Strahlengang
DE102007015063B4 (de) 2007-03-29 2019-10-17 Carl Zeiss Microscopy Gmbh Optische Anordnung zum Erzeugen eines Lichtblattes
US7789179B2 (en) 2007-05-03 2010-09-07 International Truck Intellectual Property Company, Llc Method of compression molding motor vehicle components and parts made therefrom
DE102007024757B4 (de) * 2007-05-26 2022-05-05 Carl Zeiss Microscopy Gmbh Mikroskop mit einem Mikroobjektiv und einem Zoomsystem
EP2184633B1 (fr) * 2007-09-04 2017-11-15 Nikon Corporation Système optique à agrandissement variable à relais et microscope avec celui-ci
US8711211B2 (en) * 2010-06-14 2014-04-29 Howard Hughes Medical Institute Bessel beam plane illumination microscope

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO2011036094A1 *

Also Published As

Publication number Publication date
US20120200693A1 (en) 2012-08-09
US9500849B2 (en) 2016-11-22
JP2013506150A (ja) 2013-02-21
EP3173838B1 (fr) 2022-05-18
US20150323774A1 (en) 2015-11-12
JP5635104B2 (ja) 2014-12-03
ES2923001T3 (es) 2022-09-22
EP3173838A1 (fr) 2017-05-31
DE102009044983A1 (de) 2011-03-31
US9110301B2 (en) 2015-08-18
WO2011036094A1 (fr) 2011-03-31

Similar Documents

Publication Publication Date Title
EP2480923B1 (fr) Microscope
EP2480924B1 (fr) Microscope
EP3173838B1 (fr) Microscope à feuille de lumière
EP2480926B1 (fr) Microscope
DE10241472B4 (de) Verfahren und Anordnung zur einstellbaren Veränderung von Beleuchtungslicht und/oder Probenlicht bezüglich seiner spektralen Zusammensetzung und/oder Intensität
EP2444832B1 (fr) Microscope SPIM avec nappe lumineuse STED
EP2823347B1 (fr) Microscope optique à balayage, à détection spectrale
DE102009060793A1 (de) Hochauflösendes Mikroskop und Verfahren zur zwei- oder dreidimensionalen Positionsbestimmung von Objekten
DE102012017917B4 (de) Mikroskopmodul und Lichtmikroskop sowie Verfahren und Datenspeicherträger
DE102012017920B4 (de) Optikanordnung und Lichtmikroskop
WO2005096058A1 (fr) Microscope a balayage et procede d'examen d'un echantillon au moyen d'un microscope a balayage
DE102013022538B3 (de) Verfahren zum Erstellen eines Mikroskopbildes und Mikroskopievorrichtung
DE102014008098A1 (de) Spektral flexible, schnell umschaltbare optische Filtervorrichtung
LU93022B1 (de) Verfahren und Mikroskop zum Untersuchen einer Probe
EP3475750B1 (fr) Dispositif d'éclairage pour un microscope
DE102004029733B4 (de) Rastermikroskop und Verfahren zur Rastermikroskopie
DE102013021182B4 (de) Vorrichtung und Verfahren zur Scanning-Mikroskopie
WO2023117236A1 (fr) Microscope et procédé pour microscopie
WO2008049697A1 (fr) Microscope, en particulier microscope à polarisation et/ou microscope à fluorescence

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20120123

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK SM TR

RAP1 Party data changed (applicant data changed or rights of an application transferred)

Owner name: CARL ZEISS MICROSCOPY GMBH

DAX Request for extension of the european patent (deleted)
17Q First examination report despatched

Effective date: 20160405

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: EXAMINATION IS IN PROGRESS

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20161018