EP2480922A1 - Mikroskop - Google Patents

Mikroskop

Info

Publication number
EP2480922A1
EP2480922A1 EP10754926A EP10754926A EP2480922A1 EP 2480922 A1 EP2480922 A1 EP 2480922A1 EP 10754926 A EP10754926 A EP 10754926A EP 10754926 A EP10754926 A EP 10754926A EP 2480922 A1 EP2480922 A1 EP 2480922A1
Authority
EP
European Patent Office
Prior art keywords
detection
illumination
beam path
axis
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP10754926A
Other languages
German (de)
English (en)
French (fr)
Inventor
Helmut Lippert
Matthias Wald
Christopher Power
Robert Hauschild
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Carl Zeiss Microscopy GmbH
Original Assignee
Carl Zeiss MicroImaging GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Carl Zeiss MicroImaging GmbH filed Critical Carl Zeiss MicroImaging GmbH
Priority to EP16193117.5A priority Critical patent/EP3173838B1/de
Publication of EP2480922A1 publication Critical patent/EP2480922A1/de
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/24—Base structure
    • G02B21/241—Devices for focusing
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/06—Means for illuminating specimens

Definitions

  • the sample space is adversely affected, which can have a negative effect in particular in a horizontal detection beam path. In the worst case, this also includes the removal and emptying of the sample chamber. Subsequently, a re-focusing is usually necessary. In addition, the sample is unnecessarily heated or cooled.
  • switching mirror instead of a switching mirror, it is also possible to use other switching elements, for example acousto-optical or electro-optical switching elements, which can work both on a reflective and equivalent basis on a transmissive basis. It is important compared to the integration time of the area detector - this is usually no more than 20 ms, otherwise vibrations can be negatively noticeable - short switching time, which must be such that the sample area within the integration time at least once from each Direction is illuminated. Multiple scans from any direction are also conceivable if the switchover time is small enough, for example less than 5 ms.
  • the illumination zoom element 21 and the detection zoom element 6 are connected via a control circuit for setting the Adjusting the illumination zoom element 21 in dependence on the detection image field size predetermined by the detection zoom element 6.
  • the illumination-side image field size is automatically adapted to this change when the detection image field size changed by an observer, for example, by hand.
  • the observer can therefore dispense with an illumination-side adaptation of the illumination zoom element 21; this is done by the control circuit, so that the optimum numerical aperture for the illumination is always set.

Landscapes

  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Microscoopes, Condenser (AREA)
EP10754926A 2009-09-24 2010-09-17 Mikroskop Withdrawn EP2480922A1 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP16193117.5A EP3173838B1 (de) 2009-09-24 2010-09-17 Mikroskop mit einem lichtblatt

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102009044983A DE102009044983A1 (de) 2009-09-24 2009-09-24 Mikroskop
PCT/EP2010/063665 WO2011036094A1 (de) 2009-09-24 2010-09-17 Mikroskop

Related Child Applications (1)

Application Number Title Priority Date Filing Date
EP16193117.5A Division EP3173838B1 (de) 2009-09-24 2010-09-17 Mikroskop mit einem lichtblatt

Publications (1)

Publication Number Publication Date
EP2480922A1 true EP2480922A1 (de) 2012-08-01

Family

ID=43064466

Family Applications (2)

Application Number Title Priority Date Filing Date
EP10754926A Withdrawn EP2480922A1 (de) 2009-09-24 2010-09-17 Mikroskop
EP16193117.5A Active EP3173838B1 (de) 2009-09-24 2010-09-17 Mikroskop mit einem lichtblatt

Family Applications After (1)

Application Number Title Priority Date Filing Date
EP16193117.5A Active EP3173838B1 (de) 2009-09-24 2010-09-17 Mikroskop mit einem lichtblatt

Country Status (6)

Country Link
US (2) US9110301B2 (es)
EP (2) EP2480922A1 (es)
JP (1) JP5635104B2 (es)
DE (1) DE102009044983A1 (es)
ES (1) ES2923001T3 (es)
WO (1) WO2011036094A1 (es)

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US10539772B2 (en) 2013-10-09 2020-01-21 Howard Hughes Medical Institute Multiview light-sheet microscopy
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US10181190B2 (en) * 2014-11-04 2019-01-15 Olympus Corporation Microscope and microscope image acquisition method
JP2016090766A (ja) * 2014-11-04 2016-05-23 オリンパス株式会社 顕微鏡
FR3031196B1 (fr) * 2014-12-29 2017-01-13 Karla Balaa Dispositif pour realiser de la microscopie a feuille de lumiere
JP6635052B2 (ja) * 2015-02-05 2020-01-22 株式会社ニコン 構造化照明顕微鏡、及び観察方法
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GB201507021D0 (en) 2015-04-24 2015-06-10 Isis Innovation Compact microscope
US10989661B2 (en) * 2015-05-01 2021-04-27 The Board Of Regents Of The University Of Texas System Uniform and scalable light-sheets generated by extended focusing
DE102015109645B3 (de) * 2015-06-17 2016-09-08 Carl Zeiss Microscopy Gmbh Verfahren zur Multiliniendetektion
WO2017075275A1 (en) 2015-10-29 2017-05-04 The Board Of Trustees Of The Leland Stanford Junior University Methods and systems for imaging a biological sample
US9964754B2 (en) * 2015-12-11 2018-05-08 Finger Lakes Instrumentation, Llc Method, apparatus, and computer-readable medium for switching optical paths
LU92925B1 (de) 2015-12-23 2017-09-25 Leica Microsystems Verfahren zum Untersuchen einer Probe mittels Lichtblatt-Mikroskopie und Lichtblatt-Mikroskop
ES2630733B1 (es) * 2016-01-21 2018-07-04 4D-Nature Imaging Consulting, S.L Dispositivo automático de cambio de objetivo para microscopio de haz láser plano
EP3208604B1 (en) * 2016-02-22 2019-07-10 F. Hoffmann-La Roche AG Optics for analysis of microwells
DE102016103182B4 (de) * 2016-02-23 2018-04-12 Leica Microsystems Cms Gmbh Lichtblattmikroskop und Verfahren zur lichtmikroskopischen Abbildung einer Probe
US10509215B2 (en) * 2016-03-14 2019-12-17 Olympus Corporation Light-field microscope
LU93022B1 (de) 2016-04-08 2017-11-08 Leica Microsystems Verfahren und Mikroskop zum Untersuchen einer Probe
US10876970B2 (en) 2016-04-12 2020-12-29 The Board Of Regents Of The University Of Texas System Light-sheet microscope with parallelized 3D image acquisition
DE102016108384B3 (de) * 2016-05-04 2017-11-09 Leica Microsystems Cms Gmbh Vorrichtung und Verfahren zur lichtblattartigen Beleuchtung einer Probe
AU2017281533B2 (en) 2016-06-24 2019-06-27 Howard Hughes Medical Institute Automated adjustment of light sheet geometry in a microscope
LU93143B1 (de) 2016-07-06 2018-03-05 Leica Microsystems Verfahren zum Untersuchen einer Probe sowie Vorrichtung zum Ausführen eines solchen Verfahrens
DE102017107915A1 (de) * 2016-07-18 2018-01-18 Carl Zeiss Meditec Ag System zur Augentherapie mittels Gewebebearbeitung durch nichtlineare Wechselwirkung
JP7109423B2 (ja) * 2016-08-15 2022-07-29 ライカ マイクロシステムズ シーエムエス ゲゼルシャフト ミット ベシュレンクテル ハフツング ライトシート顕微鏡
US10983327B2 (en) 2016-08-15 2021-04-20 Leica Microsystems Cms Gmbh Light sheet microscope
DE102017119169B4 (de) 2016-08-22 2023-07-27 Leica Microsystems Cms Gmbh Verfahren und Vorrichtung zur SPIM-Untersuchung einer Probe
EP3538941A4 (en) 2016-11-10 2020-06-17 The Trustees of Columbia University in the City of New York METHODS FOR FAST IMAGING OF HIGH RESOLUTION LARGE SAMPLES
US10469758B2 (en) 2016-12-06 2019-11-05 Microsoft Technology Licensing, Llc Structured light 3D sensors with variable focal length lenses and illuminators
US10554881B2 (en) 2016-12-06 2020-02-04 Microsoft Technology Licensing, Llc Passive and active stereo vision 3D sensors with variable focal length lenses
EP3332695B1 (en) 2016-12-09 2021-05-26 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Measurement device
KR102523559B1 (ko) 2017-09-29 2023-04-19 라이카 바이오시스템즈 이미징 인크. 디지털 스캐닝 장치
US20200278525A1 (en) * 2017-11-10 2020-09-03 Viventis Microscopy Sàrl Microscope for imaging a sample and sample holder for such a microscope
DE102018210603A1 (de) * 2018-06-28 2020-01-02 Carl Zeiss Microscopy Gmbh Verfahren zum Erzeugen eines Übersichtsbilds unter Verwendung eines hochaperturigen Objektivs
DE102018216038A1 (de) 2018-09-20 2020-03-26 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zur Erfassung eines Bildes eines Objekts mit einem Scanmikroskop
WO2021146280A1 (en) 2020-01-14 2021-07-22 Intelligent Imaging Innovations, Inc. Cylindrical lattice lightsheet - simplified lattice illuminator for lightsheet microscope
EP4107513A4 (en) * 2020-02-21 2024-05-29 Tornado Spectral Systems, Inc. OPTICAL SPECTROSCOPY PROBE CONFIGURATIONS TO FOCUS LIGHT ON A PART OF A SAMPLE

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Also Published As

Publication number Publication date
ES2923001T3 (es) 2022-09-22
US9500849B2 (en) 2016-11-22
US9110301B2 (en) 2015-08-18
EP3173838B1 (de) 2022-05-18
EP3173838A1 (de) 2017-05-31
JP5635104B2 (ja) 2014-12-03
WO2011036094A1 (de) 2011-03-31
DE102009044983A1 (de) 2011-03-31
JP2013506150A (ja) 2013-02-21
US20120200693A1 (en) 2012-08-09
US20150323774A1 (en) 2015-11-12

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Owner name: CARL ZEISS MICROSCOPY GMBH

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