EP2134472A1 - Dispositif pour la manipulation d'échantillons de liquide - Google Patents

Dispositif pour la manipulation d'échantillons de liquide

Info

Publication number
EP2134472A1
EP2134472A1 EP08741914A EP08741914A EP2134472A1 EP 2134472 A1 EP2134472 A1 EP 2134472A1 EP 08741914 A EP08741914 A EP 08741914A EP 08741914 A EP08741914 A EP 08741914A EP 2134472 A1 EP2134472 A1 EP 2134472A1
Authority
EP
European Patent Office
Prior art keywords
liquid
sample
flow
different
sink
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
EP08741914A
Other languages
German (de)
English (en)
Other versions
EP2134472A4 (fr
Inventor
David Bergman
Pierre KREÜGER
David RÖNNHOLM
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Amic AB
Original Assignee
Amic AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amic AB filed Critical Amic AB
Publication of EP2134472A1 publication Critical patent/EP2134472A1/fr
Publication of EP2134472A4 publication Critical patent/EP2134472A4/fr
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502746—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means for controlling flow resistance, e.g. flow controllers, baffles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
    • F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
    • F16K99/00—Subject matter not provided for in other groups of this subclass
    • F16K99/0001—Microvalves
    • F16K99/0003—Constructional types of microvalves; Details of the cutting-off member
    • F16K99/0017—Capillary or surface tension valves, e.g. using electro-wetting or electro-capillarity effects
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0887—Laminated structure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
    • F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
    • F16K99/00—Subject matter not provided for in other groups of this subclass
    • F16K2099/0082—Microvalves adapted for a particular use
    • F16K2099/0084—Chemistry or biology, e.g. "lab-on-a-chip" technology

Definitions

  • the present invention relates to the field of devices for handling liquid samples.
  • Devices for handling liquid samples of various kinds are desirable to use for instance within point of care analyses. Moreover such devices can be used to analyse various samples including of blood, plasma, serum, sweat, saliva, urine, lachrymal fluid, water samples, and suspensions or solutions of food samples.
  • Another problem is how to accurately and reproducible define a certain volume of sample which passes the analysis point before the reagent reaches the analysis point. [0010] Another problem is how to let the sample react at the analysis point for a prolonged time before the reagent reaches the analysis point.
  • a further problem is to eliminate effects from the fact the reagent may dissolve differently depending on for instance age of the reagent and how dry the reagent is.
  • the measured signal in some assays depends on the amount of reagent and the amount of sample. In many assays some kind of particles and/or some kind of molecules are detected and therefore the sample volume must be well defined.
  • a device for handling liquid samples comprising: a) projections substantially perpendicular to the surface of said device, said projections having a height, diameter and a distance between the projections capable of generating capillary flow, lateral to said surface, of a fluid, b) at least one zone for receiving a sample, c) at least one sink with a capacity of receiving said liquid, said at least one sink exerting at least two different capillary forces on said liquid, d) at least two flow paths connecting said at least one zone for receiving a sample and said at least one sink, said flow paths exerting at least two different capillary forces on said liquid, e) at least one connection between said at least two flow paths.
  • the assay device utilises projections substantially perpendicular to the surface to create a capillary force so that the liquid flows.
  • the device utilises the fact that the capillary force can be different for different flow channels depending on the distance, geometry, diameter, and height of the projections. The difference in capillary force is used to direct the flow in the desired direction.
  • Also encompassed within the present invention is a method of analysing a sample as well as a kit of parts.
  • Advantages of the present invention include that it is possible to let a sample reach an analysis point before a reagent. Another advantage is that it is possible to define a certain volume of sample that passes an analysis point before the reagent reaches the analysis point. A further advantage is that it is possible to eliminate or alleviate effects from reagents which dissolve differently.
  • Fig 1a-d depicts various embodiments of devices according to the present invention.
  • Fig 2a-d depicts various stages during use of a device according to the present invention.
  • Fig 3 is an electron micrograph of a part of an analysis device according to the present invention. It depicts a cross of two flow channels as used for instance in the device depicted in Fig 2.
  • Fig 4 depicts a section of a flow channel comprising a gate which can be used in an assay device according to the present invention.
  • Analysis point is used herein to denote a point or an area on an assay device where a measurement is performed.
  • Analyte is used herein to denote a substance, chemical constituent, or biological constituent that is analysed.
  • Reagent is used herein to denote a chemical or biological constituent participating in the analysis.
  • Sample is used herein to denote any matter comprising an analyte.
  • the assay device utilises projections substantially perpendicular to the surface to create a capillary force so that the liquid flows.
  • the assay device assay utilises the fact that the capillary force can be different for different flow channels depending on the distance, geometry, diameter, and height of the projections. The difference in capillary force is used to direct the flow in the desired direction.
  • the device for handling liquid samples comprises one zone for receiving a sample, shown as the circular zone.
  • a reactant is dried onto the substrate on the area marked with a "k”.
  • the embodiment further comprises a gate, which allows flow of liquid when it is in contact with liquid from at least two directions, indicated with a "g” in Fig 1.
  • a gate only allows a flow of liquid when it is in contact with liquid from more that one side.
  • An example of a gate which can be used in the present invention is depicted in Fig 4. It must be noted that the gates which can be used in the present invention is not limited to the gate depicted in Fig 4. Any type of gate can be used which blocks flow of liquid when it is in contact with liquid from one side only and which allows flow of liquid when it is in contact with liquid from more than one side.
  • Any type of gate can be used which blocks flow of liquid when it is in contact with liquid from one side only and which allows flow of liquid when it is in contact with liquid from more than one side.
  • the gate comprises projections with adjusted positions, distance, geometry, diameter, and height.
  • the flow in at least one of the flow paths is made slower than in the other by making the flow path longer and/or lowering the capillary force exerted on the liquid.
  • the distance, geometry, diameter, and height of the projections is adapted so that the capillary force is higher in the at least a part of the flow path and one of the sinks as compared to the flow path leading to the other sink.
  • at least one reagent is adsorbed on the surface of at least one of the flow paths.
  • the reagent is adsorbed on the flow path which exerts the lowest capillary force.
  • the flow paths exerting different capillary force on the liquid have in one embodiment different flow rates for the liquid, due to the different capillary force.
  • FIG. 3 One embodiment of the cross of flow paths in Fig 2 is depicted in Fig 3 and consists in this particular embodiment of projections with different space to create different capillary force.
  • Fig 1 a there are two different sinks exerting different capillary forces on the liquid.
  • Fig 1d there is one sink divided into two parts which are exerting different capillary force on the liquid. The border between the parts is indicated with a dashed line in Fig 1d.
  • a substance is adsorbed and/or bound to the analysis point.
  • such a substance has the capability to bind to at least one analyte.
  • examples of such a substance include an antibody.
  • at least one reagent is adsorbed or dried onto at least one flow channel.
  • Such a reagent can be any chemical or biological entity that is participating in an analysis.
  • examples of reagents include antibodies, antibodies comprising a detectable entity, other detectable molecules, and molecules with the ability to bind to an analyte.
  • Fig 1c Such an embodiment is shown in Fig 1c, where two separate reagents k and k' are added after each other.
  • at least three different capillary forces are exerted on said liquid; a first capillary force form a first sink, a second capillary force form a second sink and a third capillary force from a third sink.
  • the projections are adapted so that the exerted capillary force is different for the different sinks and flow paths.
  • an integrated analysis comprising three steps is performed.
  • a specific example of such an analysis is a device according to Fig 1c, where a first antibody directed against the analyte is bound to the analysis point.
  • the sample passes the analysis point and the analyte is bound to a fraction of the first antibodies on the surface.
  • a second antibody directed against the analyte, which antibody comprises a general binding unit passes the analysis point.
  • the second antibody will bind to the analyte which is bound to the first antibodies at the analysis point.
  • a detectable molecule with the capability of binding to the general binding unit on the second antibodies passes the analysis point.
  • the detectable molecules are bound to the antibodies.
  • a method of analysing a sample wherein the analysis is performed using a device as described above.
  • the sample is preferably added to the zone for receiving the sample.
  • the method preferably comprises a step of reading a result of the analysis.
  • kits of parts comprising the device as described above and at least one reagent.
  • the kit of parts comprises a further assay device.
  • an assay device include a holder for the device, a measurement apparatus where said device is inserted or another device which facilitates the analysis.
  • such kit of parts comprises at least one package.
  • the kit of parts comprises a written instruction.
  • the kit of parts comprises a reader capable of performing a measurement on said device. Such a reader may make a measurement on the analysis point.
  • Fig 2a Black rectangles symbolises a reactant in Fig 2.
  • the liquid flows into the zone for receiving a sample.
  • the sample flows over the analysis point where the measurement is performed. Due to the higher capillary force in one of the flow paths the liquid flows along the flow path with the highest capillary force and fills the sink with the higher capillary force. Not until the sink with the higher capillary force is completely filled, the liquid will start to flow into the region with the lower capillary force.
  • the time and the volume of sample that passes the analysis point in the first step is defined by filling the sink with the higher capillary force.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Mechanical Engineering (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

L'invention concerne un dispositif pour la manipulation d'échantillons de liquide, ledit dispositif comprenant : a) des protubérances sensiblement perpendiculaires à la surface dudit dispositif, lesdites protubérances ayant un hauteur, un diamètre et une distance entre les protubérances aptes à générer un écoulement capillaire d'un fluide, latéralement par rapport à la dite surface, , b) au moins une zone de réception d'un échantillon, c) au moins un puits ayant la capacité de recevoir ledit liquide, ledit au moins un puits exerçant au moins deux différentes forces capillaires sur ledit liquide, d) au moins deux trajets d'écoulement reliant ladite au moins une zone de réception d'un échantillon et ledit au moins un puits, lesdits trajets d'écoulement exerçant au moins deux différentes forces capillaires sur ledit liquide, e) au moins une connexion entre lesdits au moins deux trajets d'écoulement.
EP08741914.9A 2007-04-16 2008-04-15 Dispositif pour la manipulation d'échantillons de liquide Ceased EP2134472A4 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US92379807P 2007-04-16 2007-04-16
SE0700930A SE529978C2 (sv) 2007-04-16 2007-04-16 Analysanordning för vätskeformiga prov
PCT/SE2008/050424 WO2008127191A1 (fr) 2007-04-16 2008-04-15 Dispositif pour la manipulation d'échantillons de liquide

Publications (2)

Publication Number Publication Date
EP2134472A1 true EP2134472A1 (fr) 2009-12-23
EP2134472A4 EP2134472A4 (fr) 2014-10-01

Family

ID=38935813

Family Applications (1)

Application Number Title Priority Date Filing Date
EP08741914.9A Ceased EP2134472A4 (fr) 2007-04-16 2008-04-15 Dispositif pour la manipulation d'échantillons de liquide

Country Status (6)

Country Link
EP (1) EP2134472A4 (fr)
JP (1) JP5425757B2 (fr)
CN (1) CN101678355B (fr)
CA (1) CA2683920C (fr)
SE (1) SE529978C2 (fr)
WO (1) WO2008127191A1 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9216413B2 (en) 2009-07-07 2015-12-22 Boehringer Ingelheim Microparts Gmbh Plasma separation reservoir
CN103212455B (zh) 2012-01-20 2016-12-28 奥索临床诊断有限公司 在角附近具有均匀流的测定装置
JP6395999B2 (ja) 2012-01-20 2018-09-26 オーソ−クリニカル・ダイアグノスティックス・インコーポレイテッドＯｒｔｈｏ−Ｃｌｉｎｉｃａｌ Ｄｉａｇｎｏｓｔｉｃｓ， Ｉｎｃ． アッセイ装置を通じた流体流の制御
JP6278772B2 (ja) * 2014-03-19 2018-02-14 テルモ株式会社 血糖値測定用チップ
KR101996078B1 (ko) * 2014-11-28 2019-07-03 도요세이칸 그룹 홀딩스 가부시키가이샤 미세 액체 이송 구조체 및 분석 장치
CN107219170B (zh) * 2017-07-24 2023-09-19 苏州鼎实医疗科技有限公司 试剂片
WO2022058470A1 (fr) * 2020-09-17 2022-03-24 Katholieke Universiteit Leuven Mécanisme d'activation et d'équilibrage de pression

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6156270A (en) * 1992-05-21 2000-12-05 Biosite Diagnostics, Inc. Diagnostic devices and apparatus for the controlled movement of reagents without membranes
WO2003103835A1 (fr) * 2002-06-07 2003-12-18 Åmic AB Structures microfluidiques
WO2005075975A1 (fr) * 2004-02-06 2005-08-18 Nec Corporation Structure de regulation, dispositif de separation, dispositif de formation de gradient et micropuce pour leur utilisation
WO2005118139A1 (fr) * 2004-06-02 2005-12-15 Åmic AB Dispositif et procede de dosage a ecoulement regule

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0610900A (ja) * 1992-04-27 1994-01-21 Canon Inc 液体移動方法及び移動装置ならびにこれを利用した測定装置
JP3213566B2 (ja) * 1996-04-26 2001-10-02 アークレイ株式会社 検体分析用具およびそれを用いた検体分析方法並びに検体分析装置
US6296020B1 (en) * 1998-10-13 2001-10-02 Biomicro Systems, Inc. Fluid circuit components based upon passive fluid dynamics
US6571651B1 (en) * 2000-03-27 2003-06-03 Lifescan, Inc. Method of preventing short sampling of a capillary or wicking fill device
KR100444751B1 (ko) * 2002-11-11 2004-08-16 한국전자통신연구원 표면장력에 의한 유체제어 소자
JPWO2004051228A1 (ja) * 2002-11-29 2006-04-06 日本電気株式会社 マイクロチップならびにこれを用いた送液方法、質量分析システム
DE10302720A1 (de) * 2003-01-23 2004-08-05 Steag Microparts Gmbh Mikrofluidischer Schalter zum Anhalten des Flüssigkeitsstroms während eines Zeitintervalls
EP1525919A1 (fr) * 2003-10-23 2005-04-27 F. Hoffmann-La Roche Ag Dispositif de déclanchement d'écoulement
EP1525916A1 (fr) * 2003-10-23 2005-04-27 F. Hoffmann-La Roche Ag Dispositif de déclenchement d'écoulement
CN1238713C (zh) * 2003-11-01 2006-01-25 浙江大学 集成微型电磁泵式毛细管电泳芯片
GB2410086A (en) * 2004-01-14 2005-07-20 British Biocell Internat Ltd Assay devices having flow block(s) to determine flow of liquids
SE0400662D0 (sv) * 2004-03-24 2004-03-24 Aamic Ab Assay device and method
US7682817B2 (en) * 2004-12-23 2010-03-23 Kimberly-Clark Worldwide, Inc. Microfluidic assay devices
WO2006090144A1 (fr) * 2005-02-25 2006-08-31 Inverness Medical Switzerland Gmbh Dispositif de portillonnage liquide
JPWO2006098370A1 (ja) * 2005-03-16 2008-08-28 日本電気株式会社 流路の実効的な通過時間の調整機構を具える遅延回路、マイクロチップ、およびその作製方法

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6156270A (en) * 1992-05-21 2000-12-05 Biosite Diagnostics, Inc. Diagnostic devices and apparatus for the controlled movement of reagents without membranes
WO2003103835A1 (fr) * 2002-06-07 2003-12-18 Åmic AB Structures microfluidiques
WO2005075975A1 (fr) * 2004-02-06 2005-08-18 Nec Corporation Structure de regulation, dispositif de separation, dispositif de formation de gradient et micropuce pour leur utilisation
WO2005118139A1 (fr) * 2004-06-02 2005-12-15 Åmic AB Dispositif et procede de dosage a ecoulement regule

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of WO2008127191A1 *

Also Published As

Publication number Publication date
WO2008127191A1 (fr) 2008-10-23
JP5425757B2 (ja) 2014-02-26
CA2683920C (fr) 2016-05-31
SE0700930L (sv) 2008-01-22
SE529978C2 (sv) 2008-01-22
EP2134472A4 (fr) 2014-10-01
CN101678355B (zh) 2012-11-14
JP2010525319A (ja) 2010-07-22
CN101678355A (zh) 2010-03-24
CA2683920A1 (fr) 2008-10-23

Similar Documents

Publication Publication Date Title
CA2683920C (fr) Dispositif pour la manipulation d'echantillons de liquide
JP4869602B2 (ja) 被検物をマイクロ流体デバイスの多チャネルに分割する方法及び装置
US9421540B2 (en) Microfluidic device with auxiliary and bypass channels
JP4351539B2 (ja) 遠心力及び／又は毛管力によって流体を正確に移動し、操作する方法及び装置
US8877484B2 (en) Microfluidic device and a microfluidic system and a method of performing a test
JP4571129B2 (ja) 反応試薬区域に流体を均一に塗布する方法
KR100878229B1 (ko) 유체분석용 칩
JP2006208388A (ja) 試料液を検査するための装置および方法
WO2012081361A1 (fr) Appareil d'analyse et procédé d'analyse
JP2007520693A (ja) 被検体物のマイクロ流体デバイスへの取り込みならびに収納の方法および装置
WO2008047875A1 (fr) Appareil de mesure de microanalyse et procédé de mesure de microanalyse utilisant cet appareil
EP1939623A1 (fr) Procédé de mesure ultrarapide de réaction biomoléculaire
KR100907253B1 (ko) 랩온어칩 및 그 구동 방법
CA3019181C (fr) Procede et systeme dmf permettant de concentrer un analyte a partir de grands volumes en volumes plus petits a l'aide de microparticules magnetiques
US9770717B1 (en) Microfluidic chip with bead integration system
WO2008086809A1 (fr) Dispositif microfluidique et coffret pour réaliser un test
JP2010014709A (ja) アッセイ装置および方法
JP6738066B2 (ja) 抽出フィルタおよびそれを備えたイムノクロマトグラフィー分析用キット
JP2023510552A (ja) ブロッキングチャンバおよび検出チャンバを有する遠心マイクロ流体デバイス
JP2003194818A (ja) 特異結合分析方法およびこれに用いるデバイス
CN117751286A (zh) 生物传感器
JP2011128141A (ja) ラボオンチップ及びその駆動方法

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20090917

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MT NL NO PL PT RO SE SI SK TR

DAX Request for extension of the european patent (deleted)
A4 Supplementary search report drawn up and despatched

Effective date: 20140901

RIC1 Information provided on ipc code assigned before grant

Ipc: F16K 99/00 20060101ALI20140826BHEP

Ipc: B01L 3/00 20060101AFI20140826BHEP

17Q First examination report despatched

Effective date: 20151120

APBK Appeal reference recorded

Free format text: ORIGINAL CODE: EPIDOSNREFNE

APBN Date of receipt of notice of appeal recorded

Free format text: ORIGINAL CODE: EPIDOSNNOA2E

APAF Appeal reference modified

Free format text: ORIGINAL CODE: EPIDOSCREFNE

APBR Date of receipt of statement of grounds of appeal recorded

Free format text: ORIGINAL CODE: EPIDOSNNOA3E

APBX Invitation to file observations in appeal sent

Free format text: ORIGINAL CODE: EPIDOSNOBA2E

APBZ Receipt of observations in appeal recorded

Free format text: ORIGINAL CODE: EPIDOSNOBA4E

REG Reference to a national code

Ref country code: DE

Ref legal event code: R003

APBT Appeal procedure closed

Free format text: ORIGINAL CODE: EPIDOSNNOA9E

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION HAS BEEN REFUSED

18R Application refused

Effective date: 20170921