EP2027460A1 - Instrument de mesure - Google Patents

Instrument de mesure

Info

Publication number
EP2027460A1
EP2027460A1 EP07725403A EP07725403A EP2027460A1 EP 2027460 A1 EP2027460 A1 EP 2027460A1 EP 07725403 A EP07725403 A EP 07725403A EP 07725403 A EP07725403 A EP 07725403A EP 2027460 A1 EP2027460 A1 EP 2027460A1
Authority
EP
European Patent Office
Prior art keywords
cuvette
bearing
measuring device
filling
emptying
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP07725403A
Other languages
German (de)
English (en)
Inventor
Achim HÖGG
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of EP2027460A1 publication Critical patent/EP2027460A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/453—Cells therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/11—Filling or emptying of cuvettes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N2021/0367—Supports of cells, e.g. pivotable
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/05—Flow-through cuvettes
    • G01N2021/052—Tubular type; cavity type; multireflective
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44743—Introducing samples

Definitions

  • the invention relates to a measuring device according to the preamble of claim 1, in particular a measuring device for measuring in a cuvette moving particles of a sample, e.g. for measuring their velocity and, derived therefrom, their electrophoretic mobility, zeta potential and Brownian particle size, with a filling device for filling and an emptying device for emptying the cuvette, which are mounted at their ends and with means for irradiating and for observing the particles.
  • Colloids, emulsions or solid suspensions and mixtures must be kept stable and homogeneous over as long as possible.
  • the formulas for this are becoming more and more complex as the demands increase.
  • Another issue of interest in this context is targeted destabilization and separation of disperse matter to recover water from the dispersion for circulation.
  • a large part of the measures for the separation of disperse substances also runs over the particle charge, only the charge is brought in this case as possible to zero. In all cases, however, it is necessary to know the charge conditions in order to be able to control them. The zeta potential returns this charge.
  • microelectrophoresis a classical method, microelectrophoresis.
  • electrophoresis electrically charged particles of a suspension or emulsion, which are in an electrophoresis cell in the form of a cuvette, are irradiated by means of a laser and observed by means of a microscope. The images taken with the microscope are evaluated in order to deduce the speed of the particles.
  • the velocity of the particles in the electric field is in fact a measure of the electric charge whose potential can be represented and measured as a zeta potential.
  • a major problem with such measurements is an exact positioning of the measuring arrangement or its parts (laser microscope cuvette) to each other. Furthermore, it is necessary to fill the cuvette easily and also to be able to clean.
  • the invention is based on the object to show a measuring device of the type mentioned so that a simple filling and easy cleaning is possible, while the measurement accuracy should remain very high.
  • the object is achieved by a measuring device of the type mentioned above, in which the cuvette is mounted on its body by means of a cuvette bearing, wherein the filling and emptying means are fixed only to the cuvette so that the position of the cuvette relative to its environment exclusively determined or defined by the cuvette bearing.
  • An essential point of the invention is thus that the usually large and heavy equipment for filling and emptying the cuvette have no effect on the positioning of the cuvette itself.
  • the cuvette is thus used as a supporting element (contrary to its actual purpose).
  • the filling and / or the emptying devices are removably attached to the cuvette, for which purpose a fastening device is used.
  • a fastening device comprises a quick-release fastener, in particular a bayonet lock. It can be worked without tools.
  • electrodes are provided (as in the above example), they may be attached at different locations.
  • the electrodes are attached to the filling and / or the emptying device for contact with the sample, so that the cuvette can be free of all disturbing parts during cleaning.
  • the cuvette bearing comprises two partial bearings, which receive forces that run at an angle-in particular a 90 ° angle-to a longitudinal axis leading through the cuvette.
  • this may be a vertical part store and a horizontal part store.
  • These partial bearings are preferably designed as a point bearing, which allows a particularly precise adjustment.
  • These point bearings are particularly simple and precise to produce by bearing balls or bearing tips.
  • One of the two partial bearings comprises three bearing points defining a plane and supports one surface of the cuvette.
  • the other part store comprises two bearing points defining a straight line, this straight line not running perpendicular to the longitudinal axis of the cuvette. Preferably, the straight line runs parallel to this longitudinal axis.
  • the means for irradiating (the laser), as well as the means for observing (the microscope) are connected together with the cuvette bearing for defining the positions relative to each other directly or indirectly.
  • 3 is an exploded view of the cuvette with filling and emptying device in longitudinal section
  • FIG. 6 is a view along the line VI-VI of Fig. 3 and
  • FIG. 7 shows the arrangement of FIG. 3 in the assembled state in one
  • a cuvette 10 is shown schematically, through the upper cover surface of which an irradiation device 1 sends a laser beam into the interior of the cuvette, while an observation device 2 "looks" into the interior of the cuvette through the front surface perpendicular to the upper surface such that the foci meet at a predetermined point in the cuvette.
  • a cuvette bearing 40 For storage of the cuvette 10, a cuvette bearing 40 is provided, which has holders 47, 48 fastened on a base plate 46, which are here shown in an angle-shaped manner.
  • the front holder 48 in FIG. 1 has two bearing balls 43, 43 'on its vertical surface and a bearing ball 44 on its horizontal surface.
  • the other holder 47, which is at the rear in Fig. 1, has a bearing ball 43 "on its vertical surface and a bearing ball 44 'on its horizontal surface.
  • the arrangement of the bearing balls 43, 44 is also shown in Fig.
  • the position of the cuvette 10 is clearly defined up to their position in their longitudinal axis X.
  • the position in the X-axis in turn plays no role in relation to the irradiation device 1 and the observation device 2, so that here no defined position must remain secured.
  • the conditions in the assembled state are shown again in Fig. 2.
  • the cuvette is preferably pressed by a spring onto the vertical part bearing 41.
  • the cuvette 10 comprises a body 11, which is constructed in a manner known per se from plane-parallel plates. At the ends of the cuvette 10 flanges 12 and 13 are fixedly mounted, preferably sintered in a glass body. On the flanges 12 and 13 or on the line blocks 25, 35 grooves for seals 14, 15 are provided, which may be formed as O-rings. In the flanges 12, 13 are openings 16, 17, so that the cuvette 10 is open on both sides.
  • a filling device 20 can be attached to the right-hand side in FIG. 3, and an emptying device 30 can be attached to the left-hand side in FIG.
  • the flanges 12, 13 formed asymmetrically to its center axis, as is apparent in particular from FIGS. 5 and 6.
  • the filling device 20 and the emptying device 30 have fastening rings 21 and 31, whose openings are formed corresponding to the peripheral shape of the flanges 12, 13.
  • the filling device and the emptying device 30 can be placed on the covers 12, 13 as in the case of a bayonet closure and rotated relative to the cuvette 10, so that the filling device 20 as well as the emptying device 30 are firmly fixed to the covers 12 by compressing the seals 14, 15 , 13 and thus the cuvette 10 are connected.
  • the filling device 20 and the emptying device 30 comprise a first line block 25 and a second line block 35, in each of which cavities 27 and 37 are formed.
  • These cavities 27, 37 are closed to the outside by covers 23 and 33, respectively, which are fastened to the pipe blocks 25, 35 via seals 24 and 34, respectively, by means of connecting devices (not shown here), eg screws.
  • connecting devices not shown here
  • covers 23 and 33 electrodes 22 and 32 are fixed so that in the assembled state in the cavities 27, 37 protrude, wherein the electrodes 22, 32 connected via outgoing lines with a voltage source for generating an electrophoresis-effective field are.
  • the overall arrangement (according to FIG. 7) is removed from the cuvette storage 40. Then, the filling device 20 and the emptying device 30 are removed by turning relative to the cuvette 10. Now the cuvette 10 can be cleaned. Assembling is done in reverse, so just as easy.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Optical Measuring Cells (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)

Abstract

L'invention concerne un instrument de mesure destiné à mesurer des particules d'échantillons, lesquelle se déplacent dans une cuvette, par exemple destiné à mesurer le potentiel zêta ou la taille de Brown des particules. Ledit instrument de mesure comprend un dispositif de remplissage et un dispositif d'évacuation de la cuvette, ces derniers étant montés sur les extrémités de la cuvette, ainsi que des dispositifs permettant d'irradier et de surveiller les particules. L'objectif de l'invention est de positionner correctement ou de manière définie la cuvette par rapport au dispositif d'irradiation et au dispositif de surveillance. A cet effet, l'élément cuvette est doté d'un support de cuvette sur son corps, le dispositif de remplissage et le dispositif d'évacuation étant fixés exclusivement à la cuvette, de manière à ce que la position de la cuvette par rapport à son environnement est déterminée et définie uniquement par le support de cuvette.
EP07725403A 2006-05-31 2007-05-21 Instrument de mesure Withdrawn EP2027460A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE102006025392 2006-05-31
DE102006028516A DE102006028516B3 (de) 2006-05-31 2006-06-21 Küvette mit Küvettenlager und deren Verwendung
PCT/EP2007/004496 WO2007137724A1 (fr) 2006-05-31 2007-05-21 Instrument de mesure

Publications (1)

Publication Number Publication Date
EP2027460A1 true EP2027460A1 (fr) 2009-02-25

Family

ID=38462348

Family Applications (1)

Application Number Title Priority Date Filing Date
EP07725403A Withdrawn EP2027460A1 (fr) 2006-05-31 2007-05-21 Instrument de mesure

Country Status (6)

Country Link
US (1) US7884932B2 (fr)
EP (1) EP2027460A1 (fr)
JP (1) JP2009539076A (fr)
CN (1) CN101454661B (fr)
DE (1) DE102006028516B3 (fr)
WO (1) WO2007137724A1 (fr)

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Publication number Priority date Publication date Assignee Title
DE102008007743B3 (de) * 2008-02-05 2009-05-14 Particle Metrix Gmbh Verfahren und Vorrichtung zur Erfassung der Partikelverteilung in Flüssigkeiten
JP5207373B2 (ja) * 2008-09-25 2013-06-12 国立大学法人東北大学 簡易ゼータ電位測定装置及びゼータ電位測定法
JP2011149793A (ja) * 2010-01-21 2011-08-04 Ihi Corp ゼータ電位測定装置
CN101923055A (zh) * 2010-07-04 2010-12-22 肖才斌 便携式多功能比色仪
DE102012108158B4 (de) * 2012-09-03 2016-03-17 Johann Wolfgang Goethe-Universität Kapillarzelle, Anordnung und Verfahren zur Aufnahme, zur Positionierung und zur Untersuchung einer mikroskopischen Probe
US9730593B2 (en) 2013-09-25 2017-08-15 Bardy Diagnostics, Inc. Extended wear ambulatory electrocardiography and physiological sensor monitor
DE102014007355B3 (de) * 2014-05-19 2015-08-20 Particle Metrix Gmbh Verfahren der Partikel Tracking Aalyse mit Hilfe von Streulicht (PTA) und eine Vorrichtung zur Erfassung und Charakterisierung von Partikeln in Flüssigkeiten aller Art in der Größenordnung von Nanometern
CN107430061B (zh) 2015-02-17 2020-11-06 埃克斯雷姆Ip英国有限责任公司 用于温度控制旋光仪样品室的技术
CN107782670B (zh) * 2017-09-27 2019-05-28 中国科学院长春光学精密机械与物理研究所 一种比色皿测试固定装置

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DE8907526U1 (de) 1989-06-20 1989-08-31 Lambda Physik Forschungs- und Entwicklungs-GmbH, 3400 Göttingen Küvettenhalterung für einen Farbstofflaser
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Also Published As

Publication number Publication date
JP2009539076A (ja) 2009-11-12
DE102006028516B3 (de) 2007-10-04
CN101454661A (zh) 2009-06-10
WO2007137724A1 (fr) 2007-12-06
US20100128266A1 (en) 2010-05-27
US7884932B2 (en) 2011-02-08
CN101454661B (zh) 2012-07-04

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