EP1807208B1 - Anordnung zur integrierten und automatisierten dna- oder protein-analyse in einer einmal verwendbaren cartridge, herstellungsverfahren für eine solche cartridge und betriebsverfahren der dna- oder protein-analyse unter verwendung einer solchen cartridge - Google Patents

Anordnung zur integrierten und automatisierten dna- oder protein-analyse in einer einmal verwendbaren cartridge, herstellungsverfahren für eine solche cartridge und betriebsverfahren der dna- oder protein-analyse unter verwendung einer solchen cartridge Download PDF

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Publication number
EP1807208B1
EP1807208B1 EP05801513A EP05801513A EP1807208B1 EP 1807208 B1 EP1807208 B1 EP 1807208B1 EP 05801513 A EP05801513 A EP 05801513A EP 05801513 A EP05801513 A EP 05801513A EP 1807208 B1 EP1807208 B1 EP 1807208B1
Authority
EP
European Patent Office
Prior art keywords
arrangement according
cartridge
pcr
previous
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
EP05801513A
Other languages
German (de)
English (en)
French (fr)
Other versions
EP1807208A1 (de
Inventor
Heike Barlag
Siegfried Birkle
Walter Gumbrecht
Daniela KÜHN
Peter Paulicka
Manfred Stanzel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Siemens AG
Original Assignee
Siemens AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Siemens AG filed Critical Siemens AG
Publication of EP1807208A1 publication Critical patent/EP1807208A1/de
Application granted granted Critical
Publication of EP1807208B1 publication Critical patent/EP1807208B1/de
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502723—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by venting arrangements
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/11—Automated chemical analysis
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/2575—Volumetric liquid transfer

Definitions

  • the depressions may advantageously be filled with variable amounts of dry reagent. Through the combination of different dry reagent quantities and well spacing patterns, the desired concentration profiles of the final reagent solutions can be adjusted.
  • a detection module for the electrical detection of the hybridization processes is arranged in the cartridge.
  • the detection module advantageously consists of a precious metal / plastic composite or a semiconductor-processed Silicon chip with precious metal electrodes.
  • electrochemical, magnetic or piezoelectric measuring methods are suitable for electrical detection.
  • PCR for the PCR to be carried out in the cartridge according to the invention, especially for DNA analysis, there are also means for generating a magnetic field for fixing the DNA / magnetic bead complex in a PCR cavity.
  • the PCR cavity must be suitably sealed and must have means for thermal cycling.
  • reagents of this kind which in particular also contain magnetic beads for binding the released DNA, are distributed uniformly between the steps 112 via the flow channel 111.
  • Magnetic beads have DNA- and protein-binding properties, provided that they have been pretreated accordingly. They may interfere with DNA-binding properties.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Dispersion Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
EP05801513A 2004-10-15 2005-10-17 Anordnung zur integrierten und automatisierten dna- oder protein-analyse in einer einmal verwendbaren cartridge, herstellungsverfahren für eine solche cartridge und betriebsverfahren der dna- oder protein-analyse unter verwendung einer solchen cartridge Expired - Fee Related EP1807208B1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102004050576 2004-10-15
PCT/EP2005/055303 WO2006042838A1 (de) 2004-10-15 2005-10-17 Anordnung zur integrierten und automatisierten dna- oder protein-analyse in einer einmal verwendbaren cartridge, herstellungsverfahren für eine solche cartridge und betriebsverfahren der dna- oder protein-analyse unter verwendung einer solchen cartridge

Publications (2)

Publication Number Publication Date
EP1807208A1 EP1807208A1 (de) 2007-07-18
EP1807208B1 true EP1807208B1 (de) 2013-03-20

Family

ID=35432485

Family Applications (2)

Application Number Title Priority Date Filing Date
EP05803183.2A Expired - Fee Related EP1796838B1 (de) 2004-10-15 2005-10-17 Verfahren zur durchführung einer elektrochemischen messung an einer flüssigen messprobe in einer über leitungen zugänglichen messkammer
EP05801513A Expired - Fee Related EP1807208B1 (de) 2004-10-15 2005-10-17 Anordnung zur integrierten und automatisierten dna- oder protein-analyse in einer einmal verwendbaren cartridge, herstellungsverfahren für eine solche cartridge und betriebsverfahren der dna- oder protein-analyse unter verwendung einer solchen cartridge

Family Applications Before (1)

Application Number Title Priority Date Filing Date
EP05803183.2A Expired - Fee Related EP1796838B1 (de) 2004-10-15 2005-10-17 Verfahren zur durchführung einer elektrochemischen messung an einer flüssigen messprobe in einer über leitungen zugänglichen messkammer

Country Status (5)

Country Link
US (2) US7851227B2 (zh)
EP (2) EP1796838B1 (zh)
JP (1) JP4546534B2 (zh)
CN (2) CN100534619C (zh)
WO (2) WO2006042838A1 (zh)

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Also Published As

Publication number Publication date
US20090136922A1 (en) 2009-05-28
EP1796838A1 (de) 2007-06-20
CN101039750A (zh) 2007-09-19
US20090130658A1 (en) 2009-05-21
CN101039751B (zh) 2010-05-05
JP2008517259A (ja) 2008-05-22
CN100534619C (zh) 2009-09-02
JP4546534B2 (ja) 2010-09-15
EP1796838B1 (de) 2014-10-08
CN101039751A (zh) 2007-09-19
US7851227B2 (en) 2010-12-14
WO2006042838A1 (de) 2006-04-27
WO2006042734A1 (de) 2006-04-27
EP1807208A1 (de) 2007-07-18

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