EP1692515A1 - Conjugues et leur utilisation dans des procedes de detection - Google Patents

Conjugues et leur utilisation dans des procedes de detection

Info

Publication number
EP1692515A1
EP1692515A1 EP04797726A EP04797726A EP1692515A1 EP 1692515 A1 EP1692515 A1 EP 1692515A1 EP 04797726 A EP04797726 A EP 04797726A EP 04797726 A EP04797726 A EP 04797726A EP 1692515 A1 EP1692515 A1 EP 1692515A1
Authority
EP
European Patent Office
Prior art keywords
analyte
specific binding
antibody
binding
conjugate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP04797726A
Other languages
German (de)
English (en)
Inventor
Michael Trier
Carsten Schelp
Harald Ackermann
Lee Hall
Pratap Singh
Susan Swann
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Siemens Healthcare Diagnostics GmbH Germany
Original Assignee
Dade Behring Marburg GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/725,166 external-priority patent/US20050118727A1/en
Application filed by Dade Behring Marburg GmbH filed Critical Dade Behring Marburg GmbH
Publication of EP1692515A1 publication Critical patent/EP1692515A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label

Abstract

L'invention concerne des procédés servant à la détection quantitative ou qualitative d'un analyte dans un échantillon, ainsi que des réactifs appropriés, notamment une molécule porteuse à laquelle sont liés un ou plusieurs partenaires de liaison spécifiques de l'analyte et qui présente des points de liaison supplémentaires pour un partenaire de liaison X ou Y spécifique qui est associé à un constituant d'un système formateur de signal. L'invention concerne également l'utilisation d'un tel conjugué, notamment dans des tests de liaison homogènes.
EP04797726A 2003-12-01 2004-11-09 Conjugues et leur utilisation dans des procedes de detection Withdrawn EP1692515A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US52605403P 2003-12-01 2003-12-01
US10/725,166 US20050118727A1 (en) 2003-12-01 2003-12-01 Conjugates and their use in detection methods
PCT/EP2004/012647 WO2005062057A1 (fr) 2003-12-01 2004-11-09 Conjugues et leur utilisation dans des procedes de detection

Publications (1)

Publication Number Publication Date
EP1692515A1 true EP1692515A1 (fr) 2006-08-23

Family

ID=34713754

Family Applications (1)

Application Number Title Priority Date Filing Date
EP04797726A Withdrawn EP1692515A1 (fr) 2003-12-01 2004-11-09 Conjugues et leur utilisation dans des procedes de detection

Country Status (4)

Country Link
EP (1) EP1692515A1 (fr)
JP (2) JP2007512542A (fr)
CA (1) CA2547353A1 (fr)
WO (1) WO2005062057A1 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102004042124B4 (de) * 2004-08-30 2006-07-06 Dade Behring Marburg Gmbh Homogenes Verfahren zur Bestimmung eines Analyten in Gegenwart eines Überschusses von kreuzreagierenden Substanzen
CA2632261A1 (fr) * 2005-12-05 2007-06-14 Guava Technologies Caracterisation d'analyse a base particulaire
DE102007043281A1 (de) 2007-09-11 2009-05-28 Sebastian Dr. med. Chakrit Bhakdi Vorrichtung, Materialien und Verfahren zur Hochgradientenmagnetseparation biologischen Materials
CA3016361C (fr) 2016-03-07 2024-01-02 X-Zell Inc. Compositions et procedes d'identification de cellules rares
WO2023095865A1 (fr) * 2021-11-26 2023-06-01 キヤノン株式会社 Particules pour examens d'échantillon

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4434150A (en) * 1981-10-19 1984-02-28 Ortho Diagnostic Systems, Inc. Immunological reagents employing polymeric backbone possessing reactive functional groups
US4778751A (en) * 1986-05-12 1988-10-18 Diagnostic Products Corporation Method for measuring antigens or antibodies in biological fluids using ligand labeled antigens or ligand labeled antibodies
JPH0534346A (ja) * 1990-09-14 1993-02-09 Tosoh Corp 免疫測定法
DK0594772T3 (fr) * 1991-07-04 1997-02-24 Immunodex K S
JP3544962B2 (ja) * 2000-08-21 2004-07-21 エフ．ホフマン−ラ ロシュ アーゲー 改良された結合アッセイ
JP4005338B2 (ja) * 2000-11-29 2007-11-07 ペンタックス株式会社 抗体固相化担体、および、その製造方法
DE10064827A1 (de) * 2000-12-22 2002-06-27 Dade Behring Marburg Gmbh Nachweisverfahren
JP2003075443A (ja) * 2001-09-03 2003-03-12 Sekisui Chem Co Ltd 標的物質測定試薬、それを用いた標的物質の測定方法
JP2003194817A (ja) * 2001-12-26 2003-07-09 Techno Network Shikoku Co Ltd イムノクロマトグラフ分析の測定方法
JP4107873B2 (ja) * 2002-05-09 2008-06-25 賢一 花木 発光性微粒子

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
None *
See also references of WO2005062057A1 *

Also Published As

Publication number Publication date
CA2547353A1 (fr) 2005-07-07
JP2011227102A (ja) 2011-11-10
JP2007512542A (ja) 2007-05-17
WO2005062057A1 (fr) 2005-07-07

Similar Documents

Publication Publication Date Title
EP1219964B1 (fr) Méthode de détection, dans laquelle le "High-Dose-Hook Effect" est évité, réduit ou détecté
EP0363942B1 (fr) Procédé pour la détermination d'une substance liante spécifique
US20100248218A1 (en) Conjugates, and use thereof in detection methods
CH622099A5 (fr)
EP0072902B1 (fr) Procédé pour la détermination des antigènes carcinoembryonnaires (CEA) et pour la détermination appropriée de solution d'anticorps
DE2206103B2 (de) Verfahren zum Nachweisen und Bestimmen einer Komponente der Reaktion zwischen spezifisch bindenden Proteinen und den Substanzen, die von diesen Proteinen spezifisch gebunden werden
DE69930404T2 (de) Methode zum nachweis von analyten
DE3049711T1 (de) Charge effects in immunoassays
DE3136579A1 (de) Analyse fuer thyroxin und 3, 5, 3'-trijodthyronin
EP0782585B1 (fr) ELIMINATION D'INTERFERENCES DUES AU FACTEUR RHUMATOIDE AU MOYEN D'ANTICORPS ANTI-Fd
EP0349988A2 (fr) Méthode pour déterminer une substance spécifique capable de lier
JP2011227102A (ja) 複合体、及び検出方法におけるそれらの使用
EP1630175A1 (fr) Anticorps dirigés contre le polymorphisme Marburg I de la protéase activatrice du facteur VII (FSAP), leur production et utilisation
US8628933B2 (en) Homogeneous detection method
EP1787123A2 (fr) Procede homogene pour identifier un analyte en presence d'un excedent de substances a reaction croisee
EP0444561B1 (fr) Méthode pour la détermination immunologique de ligands
DE3731227A1 (de) Immunoassay-reagenzien, -kits- und -verfahren
US20050118642A1 (en) Homogeneous detection method
EP0684956B1 (fr) Agregats de proteines acyles et leur utilisation pour augmenter les signaux dans un dosage immunologique destine a la detection d'anticorps
EP3698137A1 (fr) Procédé de détermination quantitative d'un inhibiteur thérapeutique du tnf-alpha

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20060703

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LU MC NL PL PT RO SE SI SK TR

RIN1 Information on inventor provided before grant (corrected)

Inventor name: HALL, LEE

Inventor name: SCHELP, CARSTEN

Inventor name: SWANN, SUSAN

Inventor name: TRIER, MICHAEL

Inventor name: SINGH, PRATAP

Inventor name: ACKERMANN, HARALD

17Q First examination report despatched

Effective date: 20061128

DAX Request for extension of the european patent (deleted)
STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20070609