EP1638586A2 - Composition synergique destinee au traitement du diabete sucre - Google Patents

Composition synergique destinee au traitement du diabete sucre

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Publication number
EP1638586A2
EP1638586A2 EP04732676A EP04732676A EP1638586A2 EP 1638586 A2 EP1638586 A2 EP 1638586A2 EP 04732676 A EP04732676 A EP 04732676A EP 04732676 A EP04732676 A EP 04732676A EP 1638586 A2 EP1638586 A2 EP 1638586A2
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EP
European Patent Office
Prior art keywords
diabetes
treating
composition
amino acids
fenugreek
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EP04732676A
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German (de)
English (en)
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EP1638586B1 (fr
Inventor
Sunil Bhaskaran
Vishwaraman No.8 Suman Park Apartments MOHAN
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Indus Biotech Pvt Ltd
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Indus Biotech Pvt Ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
    • A61K31/19—Carboxylic acids, e.g. valproic acid
    • A61K31/195—Carboxylic acids, e.g. valproic acid having an amino group
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
    • A61K31/19—Carboxylic acids, e.g. valproic acid
    • A61K31/195—Carboxylic acids, e.g. valproic acid having an amino group
    • A61K31/197—Carboxylic acids, e.g. valproic acid having an amino group the amino and the carboxyl groups being attached to the same acyclic carbon chain, e.g. gamma-aminobutyric acid [GABA], beta-alanine, epsilon-aminocaproic acid or pantothenic acid
    • A61K31/198—Alpha-amino acids, e.g. alanine or edetic acid [EDTA]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
    • A61K31/205—Amine addition salts of organic acids; Inner quaternary ammonium salts, e.g. betaine, carnitine
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/56—Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P5/00—Drugs for disorders of the endocrine system
    • A61P5/48—Drugs for disorders of the endocrine system of the pancreatic hormones
    • A61P5/50—Drugs for disorders of the endocrine system of the pancreatic hormones for increasing or potentiating the activity of insulin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/12—Antidiuretics, e.g. drugs for diabetes insipidus

Definitions

  • the present invention relates to a synergistic composition for the treatment of diabetes mellitus, and also a process of preparing the synergistic composition.
  • Diabetes Mellitus is the most common endocrine disease. This disease is characterized by poor regulation of blood glucose levels in human beings. Blood glucose is the source of energy for basic cell functions. This glucose is driven to the cell by insulin, which is secreted by the pancreas. Diabetes Mellitus is caused by inadequate insulin secretion by the pancreas or the resistance generated by insulin receptors to the insulin. Therefore, this disease is characterized by a metabolic abnormality. Diabetes is a major metabolic disorder in which the body does not produce or properly use insulin and is characterized by hyperglycemia, glycosuria, hyperlipidemia, negative nitrogen balance and sometimes ketonemia. Diabetes is one of the most common diseases affecting human population today.
  • Diabetes Mellitus is not curable.
  • this disorder is managed by taking popular drugs available in the marketplace. These drugs fall into the following categories: - i.
  • Pancreatic stimulators - This class of drugs helps to stimulate the pancreas, leading to increased secretion of insulin. This addresses the diabetes caused by inadequate insulin secretion.
  • Insulin sensitizers - This category of drugs improves the cell's sensitivity to the presence of insulin, thereby improving the uptake of glucose into the cells, leading to better blood sugar control, iii. Insulin: - This is exogenously supplemented in the case of people suffering from both type I and type II diabetes.
  • diabetes is a lifestyle disease and cannot be cured.
  • the current therapies available therefore only offer a blood sugar management mechanism.
  • these drugs need to be taken on a sustained basis.
  • available synthetic drugs suffer from concomitant side effects caused due to long duration of usage.
  • Literature survey indicates that cardiovascular mortality was higher in patients with oral hypoglycemics than in those treated with diet and exercise alone or with insulin.
  • Sulphonylureas cause hypoglycemia as a side effect. Biguanides cause lactic acidosis.
  • Oral hypoglycemia drugs also cause GIT irritation, weight gain, hypertension, etc.
  • the diabetic person On continuous and constant exertion, the diabetic person is also liable for pancreatic fatigue.
  • many of the existing drugs available lead to drug resistance in patients with long durations of use.
  • a wide-spread pathological change is thickening of capillary basement membrane, increase in vessel wall matrix and cellular proliferation resulting in vascular complications like lumen narrowing, early atherosclerosis, sclerosis of glomerular capillaries, retinopathy, neuropathy and peripheral vascular insufficiency.
  • the level of glycosylated hemoglobin (HbAlc) is also increased in diabetes and is taken as an index of protein glycoslyation. It reflects the state of glycaemia over the preceding 2-3 months. As such, there is no drug available for the treatment of diabetic complications. Consequently, the need of the hour is to develop safe and efficacious drugs that can help in the management of blood sugar in diabetes mellitus patients.
  • Ayurveda - Ayurvedic literature refers to the usefulness of many plant extracts in the treatment of diabetes mellitus. In general, pharmacological studies over the last five decades have not brought out adequate effectiveness of such blood glucose lowering drugs. However, physicians in India who practice Ayurveda, Unani, and Siddha use extracts of leaves, flowers, fruits, seeds, wood, bark, roots, or even whole plants of more than a hundred Indian medicinal plants for the treatment of diabetes.
  • Fenugreek is used to treat diabetes, migraines, allergies and elevated cholesterol in traditional medicinal practices in India.
  • fenugreek seed is traditionally taken in a powdered form, or boiled with water, or as a sprouted seed for the control of blood sugar.
  • Fenugreek in India is used as a culinary spice and as a medicinal herb.
  • the varieties that are used for medicinal purpose have smaller grains, are dark brown in colour and bitter in taste when compared to the varieties used for culinary purposes.
  • the medicinal effect of control of blood sugar obtained with the consumption of fenugreek seed is widely varying and cannot be relied upon as a reliable agent for control of blood sugar.
  • Fenugreek seeds contain about 50% dietary fiber if used as a defatted powder. Similar to Guar Gum, fibers present in the seeds may slow gastric transit time. Slowing the rate and extent of glucose absorption in the gut is not likely to be the sole mechanism for hypoglycemic benefit.
  • Sauvaire et al isolated an amino acid present in fenugreek seeds (4 Hydroxyisoleucine) that increases glucose-induced insulin release. Fenugreek seeds contain the alkaloid trigonelline, which has a hypoglycemic effect. The following human studies demonstrate the role of fenugreek in both type 1 and type 2 diabetes therapy. Madar et al (US patent No.
  • US patent 5997877 of 1999 by Peter Chang describes a process for the fractionation of Fenugreek seeds & extraction of the various fractions such as soluble dietary fibre, deflavoured fenugreek seed, high protein fenugreek meal, etc. There is no reference to any diabetic treatment.
  • US patent 6495175 of 2002 by G. B. Rao et al describes 'a method for obtaining substantially pure fixed oil, Oleoresin & dietary fibre from fenugreek seeds. There is no mention of any diabetic treatment with any of these compounds.
  • the main object of the present invention is to develop a synergistic composition for treating diabetes.
  • Another main object of the present invention is to develop a process for the preparation of a synergistic composition from fenugreek seeds for treating diabetes.
  • Yet another object of the present invention is to develop a method of treating diabetes, using the synergistic composition obtained from fenugreek seeds.
  • Still another object of the present invention is to develop a method of treating diabetes, using Trigonelline.
  • the present invention relates to a synergistic composition for the treatment of diabetes in a subject in need thereof, said composition-comprising Trigonelline of concentration ranging between 20 to 30%, amino acids of concentration ranging between 20 to 60%, and soluble fibre of concentration ranging between 10 to 60%, optionally along with pharmaceutically acceptable additives, a process thereof and also, a method of treating diabetes.
  • Trigonelline of concentration ranging between 20 to 30%
  • amino acids of concentration ranging between 20 to 60%
  • soluble fibre of concentration ranging between 10 to 60%
  • the present invention relates to a synergistic composition for the treatment of diabetes in a subject in need thereof, said composition-comprising Trigonelline of concentration ranging between 20 to 30%, amino acids of concentration ranging between 20 to 60%, and soluble fibre of concentration ranging between 10 to 60%, optionally along with pharmaceutically acceptable additives, a process thereof and also, a method of treating diabetes.
  • the invention relates to a synergistic composition for the treatment of diabetes in a subject in need thereof, said composition-comprising Trigonelline of concentration ranging between 20 to 30%, amino acids of concentration ranging between 20 to 60%, and soluble fibre of concentration ranging between 10 to 60%, optionally along with pharmaceutically acceptable additives.
  • the amino acids are selected from a group comprising L-arginine, Leucine, Isoleucine, and 4-Hydroxyisoleucine.
  • the soluble fiber is galactomannan.
  • the additives are extracted from the same fenugreek which comprises of a group containing galactomannan.
  • the diabetes is Type II diabetes.
  • composition is obtained from plant Fenugreek.
  • the composition is free of adverse effects.
  • the invention relates to a process for the preparation of a synergistic composition from plant fenugreek comprising Trigonelline of concentration ranging between 20 to 30%, amino acids of concentration ranging between 20 to 60%, and soluble fibre of concentration ranging between 10 to 60%, optionally along with pharmaceutically acceptable additives, said process consisting steps of: flaking fenugreek seeds, extracting the flaked seeds with hydro-alcohol, concentrating the extract under vacuum to remove alcohol, extracting the concentrate with n-hexane to remove fats and lipids, diluting the defatted concentrated, filtration of the diluted extract to remove insolubles to obtain resultant, filtering the resultant through column chromatography to elute amino-acids, and
  • Trigonelline and • adding fibre of fenugreek into the amino acids and Trigonelline to obtain the synergistic composition.
  • the flakes are of size of about 15 mm.
  • hydro alcohol is isopropyl alcohol and water in equal ratio.
  • the extraction is for time duration ranging between 3 to 24 hours, preferably 12 hours.
  • amino acids are selected from a group comprising L-arginine, Leucine, Isoleucine, and 4-Hydroxyisoleucine.
  • the fibre is Galactomannan.
  • the invention relates to a method of treating diabetes in a subject in need thereof, said method comprising step of administering pharmaceutically acceptable amount of composition comprising Trigonelline of concentration ranging between 20 to 30%, amino acids of concentration ranging between 20 to 60%, and soluble fibre of concentration ranging between 10 to 60%, optionally along with pharmaceutically acceptable additives, to the subject.
  • administration of the synergistic composition is at dosage ranging between 50 to 2000 mg/kg body weight.
  • method shows hypoglycemic effect of about 50%. In still another embodiment of the present invention, wherein the method shows about
  • synergistic composition is administered orally.
  • synergistic composition acts at the pancreas through potassium channel mediated insulin secretion.
  • the invention relates to a method of treating diabetes in a subject in need thereof, said method comprising step of administering pharmaceutically acceptable amount of Trigonelline, amino acids and galactomannan fibers, optionally along with pharmaceutically acceptable additives, to the subject.
  • the additives are extracted from the same fenugreek which comprises of a group containing galactomannan.
  • the diabetes is type II diabetes.
  • composition is free of adverse effects.
  • the administration of the synergistic composition is at dosage ranging between 50 to 2000 mg/kg body weight.
  • the method shows about 300%) increase in the viability of the islets. In still another embodiment of the present invention, wherein the method maintains the morphology of the pancreatic cells.
  • Trigonelline, amino acids and galactomannan fibers act at the pancreas through potassium channel mediated insulin secretion.
  • the instant invention is not only novel but also, inventive in nature.
  • the comparative results of fenugreek seed powder (whole), and the composition of the instant application are shown below to establish inventiveness of the application.
  • the composition leads to increase in the pancreatic beta cell mass increase in a subject.
  • This is a novel aspect of the invention.
  • the beta cell mass increase is been attained.
  • This provides an indication of physiological significance of instant composition.
  • composition is not only effective in new cases of diabetes mellitus, but also, would be effective in management of patients with resistance to other generation 1, 2, and 3 drugs.
  • Fenugreek Seed is known to possess anticholesterol and antidiabetic activities. However the compounds responsible for effective antidiabetic properties have not been clearly elucidated and studied in detail in human subjects. Fenugreek Seed contains, among other, several compounds such as: 1. Host of Amino Acids
  • the present invention is about identification of individual compounds of Fenugreek seed that have beneficiary effect in the case of type 2 diabetes.
  • the present invention illustrates the method for extraction of a compound from Fenugreek seed, resulting in a combination of certain molecules that act in tandem and with synergy and leads to effective control of blood glucose in subjects suffering from type 2 diabetes.
  • the present invention explains the composition of matter of the extract derived from Fenugreek seed.
  • Soluble Fiber galactomannan The amino acids found in this seeds are L- arginine, leucine, isoleucine and 4- Hydroxyisoleucine. It was decided to work with seed screened for optimal activity. Thin layer chromatographic method was developed to screen for amino acids and Trigonelline. A quantitative method was developed for checking soluble fiber. It was decided to work with the seeds whose ratios were optimum, after establishing the amino acids content and Trigonelline content by TLC methods.
  • the seeds were subjected to flaking and hydro alcohol extraction at temperatures ranging from 20° C to 70° C and alcohol composition ranging from 50 % to 90 % (the remaining being de-mineralized water) for a period ranging from 3 hours to 24 hours, preferably about 12 hours.
  • the clear, filtered extract is concentrated to a predefined volume so as to remove maximum alcohol and diluted to a predetermined volume with water.
  • the resulting solution is subjected to fine filtration followed by Ion exchange chromatography.
  • the elutes were again passed through another weak acid Cation exchange resin in H + form to selectively remove the metabolites and impurities formed during the isolation, concentration and elution process. This part exclusively removes the smelly cyclisation products of 4 Hydroxy Isoleucine and the Keto-methyl 4 Hydroxy valeric acid.
  • the resultant elute contained only amino acids and Trigonelline.
  • the liquor obtained was spray dried on co-current equipment having a disc atomizer with a speed of 30,000 rpm.
  • the resultant powder was in a fine granular form, which can be readily formulated after standardization.
  • the ratio of the active biomolecules, which were present in the original form i.e. seed, was maintained in totality in the finished product. This was an important criterion for the activity of the said composition.
  • EXPERIMENT 1 After screening the seeds for the presence of total amino acids and Trigonelline using Thin Layer chromatography on a pre-coated Silica gel TLC plate using n-Butanol: Acetic acid: water in the ratio of 12:8:2 and initial scanning using uv at 254 nm for the presence of Trigonelline. Ninhydrin colour development was used for total amino acid presence.
  • the selected seeds in a quantity of 1 Kg were flaked in a fiaker to expose the inner core, resulting in flakes of average 15 mm in size. These flakes were than subjected to Hydro alcohol extraction using 6 litres of Isopropyl alcohol : water mixture in the ratio of 50:50 at 35° C for 12 hours.
  • the resultant liquid (about 5500 ml) was concentrated to a final volume of 150 ml under vacuum at 45-50° C. This liquid was extracted with 3 X 50 ml of n-Hexane to remove fats and lipids.
  • the defatted concentrate was diluted with de- mineralized water to a final volume of 500 ml.
  • This liquid was then subjected to fine filtration through 200-mesh size screen to remove insolubles.
  • the filtered liquid was then passed through a glass column of 500 mm length X 25 mm diameter containing 100 ml of strong acid Cation exchange resin in H + form, freshly regenerated with 600 ml of 3 % HCL in water, followed by washing to neutral pH. After passing the liquid, the column is washed with de-mineralized water to neutral pH.
  • the loaded amino acid and Trigonelline were eluted out with 200 ml of 0.5 N ammonia solution.
  • the ammonia liquid is circulated in the column until it attains a stable pH of about 8.0
  • Atomizer revolution 30,000 rpir The resultant granule from the spray drying process was found to be free flowing and suitable for formulation.
  • the resultant powder is screened in HPLC for amino acids by derivatization method using DNFB (Dinitro Flouro Benzene) and Trigonelline using UV. This contains total amino acids in the ratio of 20 to 60%>, Trigonelline from 20 to 30%> and soluble fiber (Galoctomannen) 10 to 60%>.
  • DNFB Dinitro Flouro Benzene
  • EXPERIMENT 2 A Spray dried extract of Fenugreek as explained in Experiment 1 is taken. This is filled into 00 size capsules after mixing with an excipient. The excipient is the fiber extracted from fenugreek seed. The fiber is extracted from the waste of the fenugreek seed after the extraction of the compound containing total amino acids and Trigonelline. The extraction procedure for the fiber is as below: -
  • the waste of fenugreek seed is boiled with 5 — 10 times its volume of de-mineralized water for 2 to 3 hours at a temperature of 70 - 80° C. This is subjected to coarse and fine filtration.
  • the filtered liquid is treated with activated charcoal to remove the coloring matter.
  • This liquid is vacuum concentrated to l/3 ld its original volume.
  • an alcohol to precipitate the fiber.
  • the precipitate is filtered to obtain the fiber residue.
  • the fiber residue is dried and ground to a fine powder.
  • the powdered fiber mainly contains the complex sugar from the seed in the form of Galactomannan.
  • the excipient and the active drug as extracted in Experiment No. 1 are mixed in such a way as to get a composition of total amino acids in the ratio of 20 to 60%, Trigonelline from 20 to 30% and soluble fiber (Galoctomannen) 10 to 60%>.
  • EXPERIMENT 3 A dose response study for the test drug was conducted in healthy wistar rats weighing about 100 - 150 Gms. 96 rats, which were divided into 6 equal groups, were given the test drug in different doses ranging from 250 mg/kg, 500 mg/kg, 1000 mg/kg and 2000 mg/kg, with a group kept as control rats not receiving the test drug. On the basis of this experiment, it was found that administration of a single dose of test drug at 500 mg per Kg, 1 gm/kg and 2 gms/kg produced hypoglycemic effect. The onset of action was 4 hours after administration for 500 mg per Kg, 3 hours after administration of 1 gm/kg and 1 hour after the administration of 2 gms/kg of test drug.
  • the duration of action was 4 hours for 500 mg per Kg, 7 hours for 1 gm/kg of drug and 24 hours for 2 gms/kg of the drug dose.
  • This experiment demonstrated that the drug had a good hypoglycemic effect of about 20 % at a dose of 1 gm/kg in the case of healthy wistar rats.
  • administration of 1 gm/kg of test drug for 15 days showed reduction in blood sugar level from 96.33 to 78 mgm percentage. The blood lipid profile was not altered remarkably.
  • EXPERIMENT 4 Sub-acute toxicity for this test drug was conducted in Swiss wistar rats of either sex having weights of 150 - 250 gms. 4 groups of 8 animals each were classified, with 1 as control group, and the remaining were drug treated at dosage levels 1 gm/kg body weight, 2 gm/kg of body weight and 4 gm/kg of body weight. 1. These groups were administered drugs for a period of 15 days continuously and monitored for the following: -
  • liver function test indicated normal liver function.
  • the organ weights did not show any variation and compared well with the control group. 10. Histopathology of the internal organs reveal no abnormality in the liver, kidney, spleen, stomach and lungs. On the basis of this toxicity study, it is concluded that the drug is safe in rats at the doses administered.
  • mice were induced diabetes by the administration of Alloxan. By conducting blood test on these animals, they were confirmed to have high blood sugar levels. After confirmation of diabetic induction, they were treated with the test drug at the dose of 2 gm/kg of the body weight for a period of 21-26 days. This time period was decided by the reversal of the sugar content in the urine of the mouse. The sugar content of the urine was tested everyday. The mice tested positive initially. By 21 st - 26 th day, the blood sugar in the urine tested negative during treatment with the drug. At the end of the treatment, the animals were sacrificed and their pancreas extracted for further studies. At this time, the blood samples were also taken for blood sugar check.
  • pancreas was subjected to large-scale isolation of islets by the standard procedure elucidated in the article " Large Scale Isolation of Islets " by Tissue Culture of Adult Mouse Pancreas by Y.M.Shewade, M.Umrani and R.R.Bhonde as published in Transplantation Proceedings, 31, 1721 - 1723 (1999). Isolated islets were studied to check their viability. On the basis of this experiment, it was found that the blood sugar level of mice increased to 300 -320 by the 3 ld day after the administration of Alloxan. The control group, which was not given the drug, showed a blood sugar of 480 by the 15 ( day. Many mice in this group died during the study.
  • the drug treated animals at the dose of 2 gm/kg of body weight showed a decrease in the blood sugar level to 210 mgms.
  • the viability of the islets increased from 22 % to 81 %. The observations are tabulated below: - RESULTS OF PANCREATIC CELL STUDIES
  • Islet size is smaller in Alloxan and drug group. The islets are translucent indicating there is repair arid neogenesis.
  • Ion channels consist of protein molecules designed to form water filled pores that cover the membrane and they can switch between open and closed states. Ion channels are selective and have gating properties i.e., the mechanism that controls the transition between open and closed states of the channel.
  • Oral antidiabetic drugs like sulphonylurea stimulate release of insulin from pancreas. They act on receptors on pancreatic ⁇ cell membrane. As a result of combination of sulphonylurea with receptors, there is depolarization, which means reversal change in transmembrane potential of cell by reducing conductance of ATP sensitive k+ channels. This enhances ca2+ influx, producing degranulation of ⁇ cell of pancreas.
  • EXPERIMENT 7 A prospective uncontrolled Pilot study to assess efficacy and safety of (TEST DRUG) composition derived from Experiments 1 & 2 in patients with Type II Diabetes Mellitus on treatment with Insulin. This type of patients were selected due to the viability enhancement/ neogenesis potential exhibited in mice studies Objectives:
  • the primary objectives of the study were to assess effect of TEST DRUG on fasting blood sugar levels (BSL) of Type II diabetic patients, who were on treatment with insulin and to assess the safety. Secondary objectives were to check effects of TEST DRUG on postprandial BSL, glycosylated Hb to verify whether the dosage of insulin can be reduced after administration of TEST DRUG.
  • Study Design This clinical trial was a prospective, uncontrolled, multi centered study. Study Population: 30 patients
  • Type II diabetes mellitus Fasting BSL between 100-140 mg/dl • Taking insulin for at least last 6 months
  • Safety criteria included adverse events, laboratory parameters, vital signs, physical examination and ECG examination
  • the drug is able to control blood glucose level very effectively in type 2 diabetes patients who do not respond to oral hypoglycemic agents.
  • This drug is also effective in controlling glycosilated haemoglobin content in type 2 diabetes patients This indicates effective control in managing long term complications of diabetes.
  • the composition has a property of increasing the beta cell mass and stimulating the beta cell to secrete insulin, thereby increasing the viability of beta cells and enhancing the activity of diseased pancreas.
  • the composition has a property of synergistically working together to better control blood sugar as compared to the effect of individual components.
  • the composition acts at the pancreas through potassium channel mediated insulin secretion
  • the composition controls the fasting blood sugar in type 2 diabetes patients who do not respond to oral hypoglycemic agents and who are stabilized on insulin
  • the composition controls the post prandial blood sugar in type 2 diabetes patients who do not respond to oral hypoglycemic agents and who are stabilized on insulin
  • the composition creates reduction in the dose of insulin in type 2 diabetes patients who do not respond to oral hypoglycemic agents and who are stabilized on insulin
  • the composition controls the glycosylated haemoglobin in type 2 diabetes patients who do not respond to oral hypoglycemic agents and who are stabilized on insulin
  • the composition with its properties of glycosilated haemoglobin, fasting blood sugar, post prandial blood sugar, pancreatic beta cell mass enhancement and beta cell viability enhancement leads to better control of blood sugar and thereby delays the onset of long- term diabetic complications of retinopathy, nephropathy and neuropathy in type 2 diabetes patients who do not respond to oral hypoglycemic agents and who are stabilized on insulin

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EP04732676A 2003-05-14 2004-05-13 Composition synergique destinee au traitement du diabete sucre Expired - Lifetime EP1638586B1 (fr)

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US47074203P 2003-05-14 2003-05-14
PCT/IB2004/001550 WO2004100968A2 (fr) 2003-05-14 2004-05-13 Composition synergique destinee au traitement du diabete sucre

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EP1638586A2 true EP1638586A2 (fr) 2006-03-29
EP1638586B1 EP1638586B1 (fr) 2008-03-26

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US20060134242A1 (en) 2006-06-22
DK1638586T3 (da) 2008-06-23
US7141254B2 (en) 2006-11-28
JP4353982B2 (ja) 2009-10-28
EP1638586B1 (fr) 2008-03-26
DE602004012745T2 (de) 2009-04-09
JP2006528235A (ja) 2006-12-14
US7641925B2 (en) 2010-01-05
ATE390142T1 (de) 2008-04-15
US20050008716A1 (en) 2005-01-13
WO2004100968A3 (fr) 2005-01-20
US20060153937A1 (en) 2006-07-13

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