EP1578792A2 - Nouvelle proteine associee aux centrosomes et ses applications - Google Patents
Nouvelle proteine associee aux centrosomes et ses applicationsInfo
- Publication number
- EP1578792A2 EP1578792A2 EP03813937A EP03813937A EP1578792A2 EP 1578792 A2 EP1578792 A2 EP 1578792A2 EP 03813937 A EP03813937 A EP 03813937A EP 03813937 A EP03813937 A EP 03813937A EP 1578792 A2 EP1578792 A2 EP 1578792A2
- Authority
- EP
- European Patent Office
- Prior art keywords
- protein
- seq
- polynucleotide
- asap
- sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
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- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
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- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
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- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
Definitions
- the protein according to the invention consists of the chain of 13 peptides corresponding to the translation products of 13 of the 14 exons that contains the corresponding gene, the first exon not being translated (see below).
- - Peptide 7 it comprises 24 amino acids corresponding to positions 351 to 374 (SEQ ID NO: 8); - Peptide 8: it comprises 54 amino acids corresponding to positions 375 to 428 (SEQ ID NO: 9);
- sequences SEQ ID NO: 47 to 53 corresponding to mutants of the hASAP protein deleted from the N-terminal part containing the BRCT domain (NdeM: residues 304 -647 (SEQ ID NO: 48); Ndel2: residues 411-647 (SEQ ID NO: 49); Ndel3: residues 478-647 (SEQ ID NO: 50)) or of the C-terminal part containing the MAP domain (CdeM: residues 1 to 477 (SEQ ID NO: 51); Cdel2: residues 1 to 418 (SEQ ID NO: 52); Cdel3: residues 1 to 303 (SEQ ID NO: 53); residues 1 to 421 (SEQ ID NO: 47)).
- said peptide is useful for the production of antibodies specifically recognizing a protein as defined above, preferably recognizing the ASAP protein of sequence SEQ ID NO: 1 or SEQ ID NO: 46 .
- the subject of the invention is also a kit of reagents for the implementation of the methods described above comprising: a) at least one probe or a pair of primers according to the invention; b) the reagents necessary for carrying out a conventional hybridization reaction between said probe or said primers and the nucleic acid of the biological sample; c) the reagents necessary for carrying out an amplification reaction; d) the reagents necessary for the detection and / or the assay of the hybrid formed between said probe and the nucleic acid of the biological sample or of the amplified nucleic acids formed.
- Such substances can be biological macromolecules such as for example a nucleic acid, a lipid, a sugar, a protein, a peptide, a hybrid protein-lipid compound, protein-sugar, peptide-iipid or peptide-sugar, a protein or a peptide to which chemical ramifications have been added or chemical molecules.
- the invention also relates to the polynucleotide, the protein, the antibodies, the vectors or the cells transformed according to the invention, used as medicaments.
- Hoechst 33286 (4A: 63x objective; 4B, 4C, 4D: I00X objective).
- YFP-FIS1 F (5'-AATGTCTGATGAAG I I I I IAGCACC-3 ') (SEQ ID NO: 41)
- the vectors obtained are transfected according to the calcium phosphate technique or more routinely using the jetPE1 process (GDSP10101, Qbiogene) according to the manufacturer's recommendations, in the following cell lines:
- FIG. 5 illustrates the co-location of the human ASAP protein with alpha-tubulin; similarly the murine ASAP protein co-locates with alpha-tubulin.
- RNAi interfering RNA
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Animal Behavior & Ethology (AREA)
- Immunology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pathology (AREA)
- Gastroenterology & Hepatology (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Pharmacology & Pharmacy (AREA)
- Microbiology (AREA)
- Toxicology (AREA)
- General Engineering & Computer Science (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR0216648A FR2849039B1 (fr) | 2002-12-24 | 2002-12-24 | Nouvelle proteine associee aux centrosomes et ses applications |
| FR0216648 | 2002-12-24 | ||
| PCT/FR2003/003895 WO2004058815A2 (fr) | 2002-12-24 | 2003-12-24 | Proteine associee aux centrosomes et ses applications |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| EP1578792A2 true EP1578792A2 (fr) | 2005-09-28 |
Family
ID=32406499
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP03813937A Withdrawn EP1578792A2 (fr) | 2002-12-24 | 2003-12-24 | Nouvelle proteine associee aux centrosomes et ses applications |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US7402394B2 (ja) |
| EP (1) | EP1578792A2 (ja) |
| JP (1) | JP4660200B2 (ja) |
| AU (1) | AU2003303464A1 (ja) |
| CA (1) | CA2511436A1 (ja) |
| FR (1) | FR2849039B1 (ja) |
| WO (1) | WO2004058815A2 (ja) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20120117931A (ko) | 2010-02-12 | 2012-10-24 | 온코메드 파마슈티칼스, 인크. | 폴리펩타이드를 발현하는 세포를 확인 및 분리하는 방법 |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003518364A (ja) * | 1999-05-28 | 2003-06-10 | ルードヴィッヒ インスティテュート フォー キャンサー リサーチ | 乳癌、胃癌および前立腺癌関連抗原ならびにそれらの使用 |
| US6783969B1 (en) * | 2001-03-05 | 2004-08-31 | Nuvelo, Inc. | Cathepsin V-like polypeptides |
-
2002
- 2002-12-24 FR FR0216648A patent/FR2849039B1/fr not_active Expired - Fee Related
-
2003
- 2003-12-24 CA CA002511436A patent/CA2511436A1/fr not_active Abandoned
- 2003-12-24 EP EP03813937A patent/EP1578792A2/fr not_active Withdrawn
- 2003-12-24 US US10/540,493 patent/US7402394B2/en not_active Expired - Fee Related
- 2003-12-24 JP JP2004563311A patent/JP4660200B2/ja not_active Expired - Fee Related
- 2003-12-24 WO PCT/FR2003/003895 patent/WO2004058815A2/fr active Application Filing
- 2003-12-24 AU AU2003303464A patent/AU2003303464A1/en not_active Abandoned
-
2008
- 2008-07-07 US US12/216,510 patent/US20090275024A1/en not_active Abandoned
Non-Patent Citations (1)
| Title |
|---|
| See references of WO2004058815A2 * |
Also Published As
| Publication number | Publication date |
|---|---|
| JP4660200B2 (ja) | 2011-03-30 |
| US20060216710A1 (en) | 2006-09-28 |
| US20090275024A1 (en) | 2009-11-05 |
| WO2004058815A3 (fr) | 2005-03-17 |
| JP2006526387A (ja) | 2006-11-24 |
| WO2004058815B1 (fr) | 2005-05-06 |
| US7402394B2 (en) | 2008-07-22 |
| FR2849039B1 (fr) | 2010-10-01 |
| FR2849039A1 (fr) | 2004-06-25 |
| AU2003303464A8 (en) | 2004-07-22 |
| AU2003303464A1 (en) | 2004-07-22 |
| CA2511436A1 (fr) | 2004-07-15 |
| WO2004058815A2 (fr) | 2004-07-15 |
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