EP1488157A1 - Dispositif et procede de raccordement de conduites a des systemes microfluidiques - Google Patents

Dispositif et procede de raccordement de conduites a des systemes microfluidiques

Info

Publication number
EP1488157A1
EP1488157A1 EP03708267A EP03708267A EP1488157A1 EP 1488157 A1 EP1488157 A1 EP 1488157A1 EP 03708267 A EP03708267 A EP 03708267A EP 03708267 A EP03708267 A EP 03708267A EP 1488157 A1 EP1488157 A1 EP 1488157A1
Authority
EP
European Patent Office
Prior art keywords
sealing
liquid line
fluidic
liquid
fluidic system
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP03708267A
Other languages
German (de)
English (en)
Inventor
Torsten Müller
Annette Pfennig
Stephen Shirley
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
PerkinElmer Cellular Technologies Germany GmbH
Original Assignee
Evotec OAI AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Evotec OAI AG filed Critical Evotec OAI AG
Publication of EP1488157A1 publication Critical patent/EP1488157A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
    • F16L—PIPES; JOINTS OR FITTINGS FOR PIPES; SUPPORTS FOR PIPES, CABLES OR PROTECTIVE TUBING; MEANS FOR THERMAL INSULATION IN GENERAL
    • F16L37/00—Couplings of the quick-acting type
    • F16L37/02—Couplings of the quick-acting type in which the connection is maintained only by friction of the parts being joined
    • F16L37/04—Couplings of the quick-acting type in which the connection is maintained only by friction of the parts being joined with an elastic outer part pressing against an inner part by reason of its elasticity
    • F16L37/05—Couplings of the quick-acting type in which the connection is maintained only by friction of the parts being joined with an elastic outer part pressing against an inner part by reason of its elasticity tightened by the pressure of a mechanical element

Definitions

  • the invention relates to devices for coupling fluid lines to fluidic microsystems, in particular a coupling device for fluid-tight coupling of at least one fluid line to a fluidic system, fluidic systems equipped with such devices, and methods for line coupling to fluidic microsystems.
  • fluidic systems are used to handle suspended biological or synthetic samples.
  • miniaturized fluidic systems microfluidic systems, fluidic microsystems
  • Fluidic microsystems are particularly suitable for sample-specific single cell treatment or measurement and may be equipped with microelectrode devices for this purpose.
  • a fluid microsystem is typically produced as a compact component (so-called chip). The following techniques are known from practice for loading the microsystem with the respective samples (e.g. biological cells, cell components, synthetic particles and / or liquid media).
  • the fixed attachment of tubes to microsystems is disadvantageous, since in most applications a flexible adaptation of the microsystem to the sample supply and separate handling of the tubes and the microsystem, e.g. B. for cleaning purposes, is desired.
  • the plug-in or screw connections on the other hand, have fluidic disadvantages, since an undesired dead volume is formed at the place of a plug-in or screw adapter, in which a change in the flow cross-section also takes place in comparison to the connected hose.
  • a dead volume causes several problems.
  • a quantitative sample entry or a quantitative sampling with small cell numbers and / or small sample volumes (e.g. ⁇ 10 ⁇ l, ⁇ 1000 cells / ⁇ l) is made more difficult or impossible.
  • the applications of conventional hose couplings are limited to microsystems in which volumes in the higher ⁇ l to ml range can be taken up as storage volume and the flow velocities and volume flows are in the range> 100 ⁇ l / h and the velocities in the range> 500 ⁇ m / s Recovery rate for the sample examined is not of significant interest. However, this represents a considerable restriction of the area of application of conventional microsystems.
  • each dead volume is associated with extended pumping times.
  • a hose with an inner diameter of approx. 250 ⁇ has a volume of approx. 2 ul.
  • a desired flow rate of e.g. B. 10 ⁇ l / h results in a residence time of approx. 10 mins.
  • the dead volume is also associated with a change in the flow cross-section, e.g. B. an expansion connected to a connection adapter.
  • the flow velocity is reduced.
  • Samples or sample components can settle (sedimentation). For example, there may be an undesirable loss of cells or a delay until the cells continue to be rinsed.
  • Dead volumes therefore also create a risk of contamination build-up, which can lead to susceptibility to germs.
  • a coupling device for microfluidic applications is known from WO 99/63260.
  • a hollow body is fixed on a fluidic chip and an O-ring seal is integrated in its end facing the fluidic chip via an opening in the fluidic chip.
  • a liquid line with a profiled outer wall is inserted into the hollow body with the O-ring seal.
  • the free end of the liquid line is pushed towards the opening until the profiled outer wall of the liquid line sits in the O-ring seal.
  • the O-ring seal is radially compressed in the hollow body, a liquid-tight connection being formed between the liquid line and the fluidic chip.
  • the coupling device according to WO 99/63260 has several disadvantages.
  • the coupling device can only be used with liquid lines with a profiled line end.
  • the end of the line may have to be processed before use (e.g. by machining or heat treatment).
  • a further disadvantage arises when, by inserting the end of the liquid line into the fluid chip in the respective opening of the fluid chip, the thickness of the wall material of the liquid line creates a step by which the dead volume is formed with the disadvantages described above.
  • Another problem is that the conventional technology is designed for relatively high operating pressures (for example 70 bar), but which are impractical in fluidic microsystem technology, in which fragile glass chips are used, for example.
  • a major disadvantage is that, according to WO 99/63260, a good seal is achieved between the liquid line and the radially clamped O-ring.
  • the sealing function of which is unreliably fulfilled because of its small expansion.
  • the surface of the fluid chip is loaded unevenly. Higher demands are placed on the stability of the fluidic chip. If correspondingly thicker wall materials are used, there are disadvantages for the applicability of optical measurement methods on the fluidic chip.
  • Small cell counts in the range of 1 to 500 cells should be flushed through the microsystem with a recovery rate> 70% and evaluated and manipulated according to various criteria (e.g. size, dielectric properties, optical properties, fluorescence properties).
  • Typical pump speeds in the range of 100 to 500 ⁇ m / s or pumping rates in the range of 2-20 ⁇ l / h should be realized.
  • the object of the invention is to provide improved devices for coupling liquid lines to fluidic microsystems, with which the disadvantages of conventional coupling techniques are overcome.
  • the devices are to be characterized in particular by an expanded area of application, high flexibility and improved flow properties, such as, for. B. feature a minimal dead volume and avoidance of steps in the flow cross-section.
  • the object of the invention is also to provide improved methods for coupling liquid lines to fluidic microsystems, in particular using such devices.
  • a basic idea of the invention is to provide a coupling device for the liquid-tight coupling of at least one liquid
  • a coupling device for the liquid-tight coupling of at least one liquid
  • a fluidic system in particular to a fluidic microsystem, which has at least one sealing device at which the liquid line ends and the at least one sleeve with a first planar sealing surface for resting on an outer surface of the fluidic system through which the end of the liquid line points to an opening in the outer surface, and comprises at least one clamping device with which the sealing device can be pressed against the fluidic system, so that the first sealing surface enters into a liquid-tight connection with the outer surface of the fluidic system.
  • the provision of a sealing device with a sealing surface radially surrounding the end of the liquid line has the advantage that the liquid line can be coupled directly to the fluidic system without a dead volume.
  • the liquid line opens directly into the microsystem without an intermediate adapter.
  • the clamping device creates a releasable connection between the liquid line and the fluidic system, which is advantageously suitable for sealing even at elevated pressures, and thus enables high flow velocities even with small flow cross sections, without the fluidic system being influenced by mechanical pressing.
  • the coupling device according to the invention is characterized by simplified handling.
  • the liquid line equipped with the sealing device can be placed on its outer surface for coupling to a fluidic system, the end of the liquid line being arranged over a selected opening in the outer surface, and fixed by a simple actuation of the clamping device.
  • the clamping device has at least one hollow plunger which is movable relative to the outer surface of the fluidic system, so that the movement towards the fluidic system onto the at least one sleeve of the sealing device causes one to face the outer surface of the fluidic system. directed force can be exerted.
  • the at least one sleeve of the sealing device has an outer shape which is formed such that the desired force is exerted on the sealing surface under the action of the hollow plunger.
  • the clamping device comprises at least one hollow punch which has at least one receptacle for at least part of the sleeve of the sealing device and, if appropriate, an end face with which the sealing device can be pressed onto the fluidic system.
  • a hollow plunger has the particular advantage that the contact pressure for fixing the sealing device on the outer surface of the fluidic system can be distributed uniformly and can be chosen to be so small in relation to the sealing surface that the fluidic system is not deformed or possibly destroyed.
  • a plurality of liquid lines, which can be connected to form one or more sealing devices can advantageously be held in place with a plurality of sleeves simultaneously and in a space-saving manner with respectively associated hollow punches.
  • the sealing device can be formed integrally with the end of the liquid line or can be firmly connected (eg glued). According to preferred embodiments of the invention, however, the liquid line and the sealing device form separate, detachable components which can be reversibly connected to one another.
  • the sleeve of the sealing device has an inner hollow channel which is designed to releasably receive an end region of the liquid line and forms a second sealing surface, the sealing device with the clamping device being able to be pressed onto the end region of the liquid line, so that the second sealing surface with the surface of the end region the liquid line enters into a liquid-tight connection.
  • the sealing device advantageously fulfills a double function.
  • the end of the fluid line is sealed laterally (or radially) from the outer surface of the fluidic system and according to the orientation of the fluid line (or axially) along the surface of the fluid line.
  • the detachable sealing device has the additional advantage of an increased flexibility of the coupling device.
  • the sleeve can easily be plugged into a hose end and fixed to a fluidic system with the clamping device, in particular the hollow punch.
  • the length of the liquid line can be optimally adjusted in advance to the geometric conditions in the specific application. Hose lengths can be reduced and pumping times can be shortened.
  • the hollow punch of the clamping device for the sleeve of the sealing device forms a conical or a cylindrical receptacle, the maximum inside diameter of which is smaller than the outside diameter of the sealing device.
  • a cylindrical receptacle has the advantage of evenly pressing the sealing device onto the end of the liquid line.
  • the at least one sealing device is equipped with a plurality of sleeves with which a plurality of liquid lines can be coupled to the fluidic system.
  • the sleeves can be connected in rows or in a matrix in one or more sealing units.
  • the first sealing area is larger than the cross-sectional area of the end of the liquid line, there may be advantages for the tightness even with a low contact pressure of the clamping device.
  • Another object of the invention is a fluidic system which is equipped with at least one coupling device according to the invention.
  • the fluidic system has a chip body, to which at least one liquid line is connected to the coupling device.
  • the chip body has an at least partially planar outer surface in which at least one opening is formed, the liquid line preferably being seated on the planar outer surface with its line end.
  • the dead volume of the coupling can thus advantageously be minimized.
  • the line end of the liquid line preferably has the outer shape of a circular cylinder, non-profiled tubes or capillaries can advantageously be used as liquid lines without additional processing steps. No special accuracy requirements have to be placed on the outside diameter of liquid lines.
  • the fluidic system according to the invention comprises a fluidic microsystem. The requirements of fluidic microsystems with regard to a mechanically gentle handling and the possibility of measurements in the microsystem also in the vicinity of the connected lines are optimally met by the combination with the coupling device according to the invention.
  • the invention also relates to a method for the liquid-tight coupling of at least one liquid line to a fluidic system, in particular with a coupling device according to the invention.
  • the method is characterized by a sequence of steps in which at least one liquid line with a sealing device and the clamping device is coupled to the fluidic system, so that the end of the liquid line is aligned with an opening in the outer surface of the fluidic system, a contact force being formed on the clamping device in this way is that the sealing device forms the liquid-tight connection with the outer surface of the fluidic system.
  • the method according to the invention has the advantage of a simple and universal application in various types of liquid lines that are of interest in practice. Liquids, e.g. B. particle suspensions, dead volume free, d. H. introduced directly from the liquid line (hollow body) into the microfluidic system.
  • the invention has the following further advantages.
  • the coupling device according to the invention is simple to use for the user.
  • the flat design of the first sealing surface results in a large contact surface with the outer surface of the fluidic system, as a result of which an optimal seal is achieved.
  • the coupling device is characterized by a uniform pressure distribution tion and thus a low mechanical load on the fluidic system, in particular a fluidic microsystem. Tightness is guaranteed even with increased internal pressures. There is a reliable seal even with internal pressures of up to 0.1 MPa, for example.
  • the coupling device is reversible and easy (ie without tools) and user-friendly detachable.
  • the entire coupling device, parts of it or a combination of the coupling device and the lines can be manufactured as disposable items or can be sterilized by a suitable method.
  • FIG. 3 a perspective view of an embodiment of the coupling device according to the invention, which is designed for coupling several liquid lines,
  • Fig. 5 two views of a clamping device
  • FIG. 9 a graphical representation of test results obtained with a coupling device according to the invention.
  • a liquid line is a hollow body in which a liquid sample is arranged and which is set up for introducing the sample into the fluidic system.
  • the liquid line can in particular be a tube, a capillary, a part of a syringe, or a connection to a reservoir of a microtiter plate or to a liquid delivery device.
  • FIG. 1 partially illustrates a first embodiment of the coupling device 100, which is set up for coupling a liquid line 10 to the microsystem 20 with a sealing device 30.
  • Fig. 1 is a schematic illustration, the details and proportions may vary in practice.
  • the liquid line 10 is, for example, a hose made of plastic Fabric material, e.g. B. PTFE, PEEK, polypropylene, polyethylene, PVC, silicone, or a capillary made of glass, metal or a metal alloy.
  • plastic Fabric material e.g. B. PTFE, PEEK, polypropylene, polyethylene, PVC, silicone, or a capillary made of glass, metal or a metal alloy.
  • the material is selected depending on the application and is preferably inert (cell compatible), sterilizable and not very cell-adhesive with respect to the samples to be treated.
  • the inside diameter of the liquid line 10 is approx. 250 ⁇ m. In cell biological applications, the inner diameter is preferably in the range from 120 ⁇ m to 200 ⁇ m or larger.
  • the outer diameter of the liquid line 10 is, for example, 1.6 mm.
  • the microsystem 20 which is shown schematically only in part in FIGS. 1 and 2, is formed by a channel or compartment structure in a solid body (chip).
  • the channels 21 of the microsystem have dimensions that are typically in the range from 5 to 1000 ⁇ m (width), 5 to 1000 ⁇ m (height) and from 1 to 100 mm (length).
  • biological or synthetic samples e.g. B. cells, cell components, macromolecules, plastic particles or the like. Analyzed, manipulated, separated and / or microscopically evaluated (see Müller et al. In "Biosensors & Bioelectronics, Vol. 14, 1999, pp. 247-256).
  • the microsystem 20 is in a manner known per se with measuring and / or manipulation devices, in particular with a microelectrode device.
  • the microsystem consists, for example, of a semiconductor material (for example silicon), plastic or glass or one or a composite of at least two of these materials.
  • the device (not shown) for dielectrophoretic manipulation and / or measurement of particles.
  • the chip body of the microsystem 20 has an outer surface 22 that is at least sectionally flat.
  • FIGS The flat outer surface 22 is provided with openings 23 through which the structure of the channels 21 or compartments can be accessed.
  • the number and arrangement of openings 23 is selected depending on the task in the design of the microsystem. For example, a single opening 23 is shown in Fig. 1, which has a diameter of z. B. 500 microns and the coupling of a suspension sample from the liquid line 10 in the channel 21 is used. Generally, the opening forms an inlet or outlet in the wall of the fluidic system. In the vicinity of the opening or bores 23, the outer surface 22 has a flat, smooth surface. Most chip materials have a smooth surface.
  • the sealing device 30 comprises a conical sleeve 32, on the wider end surface (bottom side in FIG. 1) of which the first sealing surface 31 is formed.
  • the sleeve 32 has a lower projection 33.
  • the projection 33 on the one hand enlarges the first sealing surface 31 and on the other hand creates an engagement surface for the clamping device 40 (see FIG. 2).
  • the projection 33 is not a mandatory feature of the invention.
  • the sealing function can also be formed with a simply conical sleeve 32 or with a suitable inner shape of the clamping device 40 by a sleeve in the form of a straight cylinder.
  • the outer shape of the sleeve 32 and the inner shape of the clamping device 40 are formed such that a force can be exerted at least towards the outer surface of the microsystem.
  • the first sealing surface 31 has a dimension of at least 10 mm 2 , preferably 20 mm 2 .
  • the sealing device 30 consists of an elastic plastic material, such as. As silicone material, rubber or another elastic plastic, which is preferably sterilizable, not swellable, and is biologically harmless.
  • the material is preferably so soft that in cooperation with the Clamping a seal is made possible without deforming or destroying the chip body. For example, it has a hardness in the range 30-50 Shore A.
  • Materials are preferably used which have a high resistance to temperature, solvents (e.g. organic solvents such as ethanol) and nonionic, anionic and cationic surfactants and / or which sterilize the device by autoclaving (e.g. 20 minutes Allow 121 ° C in the tensioned steam at 2 bar).
  • the sleeve 32 has a hollow channel 34, which is designed to releasably receive the end region 11 of the liquid line 10.
  • the hollow channel 34 forms a second sealing surface 35, which represents a contact surface of the sealing device 30 with the end region 11.
  • the conical second sealing surface 35 has a dimension of at least 10 mm 2 , preferably 20 mm 2 .
  • the inner diameter of the hollow channel 34 is preferably selected such that it is at most as large as the outer diameter of the end region 11, but is preferably slightly smaller.
  • the sealing device 30 is pressed onto the hose 10 and against the microsystem 20 with the clamping device 40, as is illustrated schematically in FIG. 2.
  • the clamping device 40 comprises a hollow punch 41, which can be pressed against the microsystem 20 with a schematically shown clamping mechanism 42.
  • the underside of the hollow punch 41 is at a distance from the outer surface 22.
  • the clamping mechanism 42 is actuated (for example a bayonet connection, see FIG. 7), the distance from the hollow punch 41 from the outer surface 22 is reduced.
  • the associated exertion of force takes place according to the arrows perpendicular to the outer surface 22.
  • the hollow punch 41 forms a conical receptacle 43, the inner shape of which rests on the outer shape of the sleeve 32 is adjusted.
  • the contact area between the inner and outer shapes has a dimension of at least 10 mm 2 , preferably 33 mm 2 .
  • Figures 1 and 2 show as a particular advantage of the coupling device according to the invention that the end 12 of the liquid line 10 directly adjoins the opening 23 of the channel 21. Samples are transferred from the liquid line 10 into the channel 21 without dead volume. The liquid line 10 opens directly into the channel 21 without the interposition of adapters or the like.
  • the coupling according to the invention with the coupling device 100 takes place according to one of the following procedures, depending on the application and structure of the clamping device 40.
  • the coupling device according to the invention makes it possible, as an alternative, first to put the sealing device 30 alone over the opening 23 with the clamping device 40, but without pressing the clamping device 40 onto the microsystem 20. In this state, the end region 11 of the liquid line 10 can be pushed into the sleeve 32 and then the clamping device 40 can be tightened. be. This procedure is particularly advantageous when using sealing units, which are described below with reference to FIGS. 3 to 6.
  • a modified embodiment of the coupling device 100 according to the invention is shown in perspective in an exploded view in FIG. 3.
  • a plurality of liquid lines 10 are coupled to a fluidic microsystem 22, two sealing units 36 being separated from one another as sealing devices 30 and a fluidic block 45 being provided as a clamping device 40.
  • the liquid lines or hollow bodies 10 are used to guide one or more externally induced liquid flows into and / or out of the microfluidic system independently of one another.
  • the microsystem 20 comprises the chip body 24, on which a holding plate 25 is placed.
  • the chip body 24 contains the channel or compartment structure with a microelectrode device, from which electrical contacts 26 are guided to the edge of the chip body 24.
  • the chip body 24 consists, for example, of a glass composite with a plurality of fluidic openings, each of which corresponds to the opening 23 in FIG. 1. For example, eight fluidic openings with a diameter of 500 ⁇ m each are provided.
  • the holding plate 25 is provided on the upper side of the chip body 24 and has two cutouts 27 for receiving a sealing unit 36 and an observation window 28 through which the glass chip body 24 is exposed.
  • each sealing unit 36 which is illustrated in an enlarged manner in FIG. 4, comprises four conical sleeves 32, which are each constructed analogously to the sealing device 30 according to FIG. 1 and are connected to one another via the continuous projections 33 as a row.
  • the continuous projections 33 form a sealing mat.
  • the spacing of the sealing sleeve 32 protruding from the sealing mat in the sealing unit 36 corresponds precisely to the spacing of the fluidic openings in the chip body 26.
  • the sealing mat has the particular advantage that the pressing forces generated by the clamping device are transmitted uniformly to the outer surface of the chip body 24.
  • the fluidic block 45 which is shown in more detail from two sides in FIG. 5, fulfills the function of the clamping device 40. It consists of a carrier plate 46, on the side facing the microsystem 20, two rows of hollow punches 47 are provided, which simultaneously serve for hose guides and receptacles form for the sealing units 36.
  • the fluidic block 45 is preferably made of metal, metal alloys, plastics, such as. B. Teflon, PEEK, KEL-F, or ceramics.
  • the sealing units 36 are inserted into the rows of hollow punches 47. This can be done manually with little pressure.
  • the fluidic block 45 is then placed on the microsystem 20.
  • the undersides of the sealing units 36 are received by the cutouts 27 in the holding plate 25.
  • the fluidic block 45 and the microsystem 20 are connected to one another with a mechanical structure (for example: bayonet connection, see FIG. 7).
  • the liquid lines 10 are then introduced into the hollow channels of the sealing devices and the fluidic block 45 is pressed against the microsystem.
  • the liquid-tight composite is advantageously produced simultaneously for all liquid lines. In the event that one or more fluidics openings should not be coupled to a line, massive packing, z. B. in rod form.
  • FIG. 6 A modified design of the coupling device according to FIG. 3 is illustrated in FIG. 6.
  • a chip carrier (so-called pillar) 48 is shown, which interacts with the fluidic block 45.
  • the reference numeral 29 refers to a circuit board adapter which interacts with the electrical contacts 26 of the chip body for the electrical control of the microsystem.
  • the chip body 24 is connected (for example glued) to the holding plate 25.
  • the holding plate 25 serves to increase the strength of the chip body and the cooling (heat sink).
  • the holding plate 25 is screwed onto the chip carrier 48. It has two parallel elongated holes corresponding to the above-mentioned recesses 27, between which the observation window 28 is located.
  • the sealing units 36 arranged between the chip carrier 48 and the chip body 24 fulfill two tasks, namely the reception of the liquid lines 10 and the sealing of the End sections of the liquid lines.
  • the microsystem 20 is set up to analyze, separate and / or isolate molecules or particles in liquids. For example, micro objects such as cells and artificial particles in the order of magnitude of usually 2 ⁇ m to 100 ⁇ m are to be analyzed, manipulated, pored, separated and / or evaluated microscopically.
  • the microsystem 20 forms a sorter, for example.
  • the chip body contains at least at least a channel with a sorting device, as is known per se in fluidic microsystems. They are based, for example, on the dielectric separation of particles with different properties measured in the microsystem. A suspension with a particle mixture is introduced into the channel via a liquid line.
  • the sample is introduced accelerated by a sheath flow, which has a flow rate of up to 2000 pl / s, for example.
  • a sheath flow which has a flow rate of up to 2000 pl / s, for example.
  • two partial streams are discharged from the microsystem, each of which is accelerated with an enveloping stream for accelerated decoupling.
  • a bayonet connection 42 is shown, by means of which the fluidic block 45 and the holding plate 25 are connected to one another.
  • the bayonet connection 42 simultaneously forms a coupling and the clamping mechanism shown schematically in FIG. 2, with which the distance between the components mentioned can be reduced and the contact pressure can thereby be exerted.
  • the bayonet connection 42 comprises a bayonet ring 42.1 with two anchoring ramps 42.2 and a slot 42.3.
  • the slot 42.3 advantageously enables the bayonet ring 42.1 to be fitted when the tubes 10 have already been inserted into the sealing units 36 by an external additional device (not shown, for example sample reservoir, pump). In this case, the tubes 10 are threaded through the slot 42.3 into the bayonet ring 42.1.
  • the anchoring ramps 42.2 interact with two anchor pins 25.1 which protrude from the holding plate 25.
  • the pressure of the fluid block 45 against the fluidic chip 24 with the holding plate 25 is achieved when the bayonet connection 42 is locked. This is preferably between the bayonet ring 42.1 and the holding plate 25, a spring ring (not shown) is provided. Alternatively, the movement of the bayonet ring 42.1 towards the holding plate 25 can be adjusted by the design of the anchoring ramps 42.2.
  • the fluidic block 45 is equipped with guide pins 45.1, which serve to guide and align the bayonet ring 42.1.
  • the guide pins 45.1 comprise projections which are arranged at the corners of the surface of the fluidic block 45.
  • the holding plate 45 is provided with lateral recesses 45.2 through which the anchor pins 25.1 of the holding plate 25 can protrude.
  • the sealing units 36 are first placed in the cutouts 27 of the holding plate 25 on the chip body 24 or the sealing units 36 are inserted into the hollow punch rows 47 of the fluidic block 45 and then the fluidic block 45 is placed on the holding plate 25.
  • Commercially available hoses are inserted into the addressed openings in the fluidic block.
  • hoses made of PTFE OD 1/16 ''
  • the bayonet ring 42.1 is put on and locked (for example by half a turn).
  • the fluidic block 45 is pressed onto the chip body 24 by the locking movement and the desired sealing of the inserted tubes is thus achieved.
  • the in Figs. Embodiments of the invention shown in Figures 7 and 8 have the following further advantages.
  • the bayonet connection 42 is easy to handle.
  • the fluidic block is reversibly attached to the chip so that, in particular, the tubes and the conical sealing elements are replaced. is made possible.
  • the hoses and the chip do not require any special processing (grooves, etc.) to be sealed, and they can be inserted into the attached fluidic block and do not have to be inserted before assembly.
  • the risk of breakage for the chip when placing the fluidic block can be reduced to a minimum.
  • the fluidic block is adjusted by guides mounted on the chip. Tilting of the fluidic block by turning the bayonet lock is prevented by the guide pins 45.1.
  • FIG. 9 The result of a test of the coupling device according to the invention is illustrated in FIG. 9.
  • the speed in the channel of the microsystem was measured as a function of the pressure in a sheath flow container, with which the speed of the sheath flow is set. With increasing pressure, there is only a slight fluctuation in the flow velocity in the channel. The current in the channel is negligibly influenced by the increase in the flow velocity of the enveloping currents. This proves the high tightness of the coupling device according to the invention. In contrast to this, a strong dependence of the flow velocity in the channel on the flow rate of the sheath flow was observed when testing a conventional coupling device with screw adapters.

Abstract

L'invention concerne un dispositif de couplage (100) notamment destiné au raccordement étanche aux liquides d'au moins une conduite de liquides (10) à un système fluidique (20). Ledit dispositif de couplage est composé d'au moins un système de joint (30) conçu pour recevoir une extrémité (11) de la conduite de liquides (10), et comportant une première surface de joint (31) destinée à s'appuyer sur une surface extérieure (22) du système fluidique de manière que l'extrémité de la conduite de liquides (10) est entourée latéralement par la première surface de joint (31) et pointe vers une ouverture (23) pratiquée dans la surface extérieure (22). Ledit dispositif de couplage est par ailleurs composé d'un système de blocage (40) avec lequel le système de joint (30)peut être comprimé contre le système fluidique (20) de manière que la première surface de joint (31) est raccordée de façon étanche aux liquides à la surface extérieure (22).
EP03708267A 2002-03-25 2003-03-25 Dispositif et procede de raccordement de conduites a des systemes microfluidiques Withdrawn EP1488157A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE10213272 2002-03-25
DE10213272A DE10213272A1 (de) 2002-03-25 2002-03-25 Vorrichtung und Verfahren zur Leitungsankopplung an fluidische Mikrosysteme
PCT/EP2003/003092 WO2003081113A1 (fr) 2002-03-25 2003-03-25 Dispositif et procede de raccordement de conduites a des systemes microfluidiques

Publications (1)

Publication Number Publication Date
EP1488157A1 true EP1488157A1 (fr) 2004-12-22

Family

ID=28050821

Family Applications (1)

Application Number Title Priority Date Filing Date
EP03708267A Withdrawn EP1488157A1 (fr) 2002-03-25 2003-03-25 Dispositif et procede de raccordement de conduites a des systemes microfluidiques

Country Status (5)

Country Link
US (1) US7475916B2 (fr)
EP (1) EP1488157A1 (fr)
AU (1) AU2003212378A1 (fr)
DE (1) DE10213272A1 (fr)
WO (1) WO2003081113A1 (fr)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003076119A1 (fr) * 2002-03-12 2003-09-18 Hamamatsu Photonics K.K. Procede de decoupe d'objet traite
DE10304653B4 (de) * 2003-02-05 2005-01-27 Evotec Technologies Gmbh Mehrparametrige Detektion in einem fluidischen Mikrosystem
DE10320956B4 (de) * 2003-02-05 2005-02-17 Evotec Technologies Gmbh Untersuchungsverfahren für biologische Zellen und zugehörige Untersuchungseinrichtung
DE10320871B3 (de) * 2003-05-09 2004-09-16 Evotec Technologies Gmbh Probenablageeinrichtung für einen Zellsortierer
DE502004002667D1 (de) * 2004-01-13 2007-03-08 Frape Behr Sa Befestigungsvorrichtung für ein Expansionsventil einer Kraftfahrzeugklimaanlage
DK2069673T3 (da) 2006-08-12 2013-11-04 Pelt Colleen K Van Nanoliter-flowrate-separations- og elektrosprayanordning med plug and play-trykforbindelser og diagnoseovervågningssystem med flere sensorer
EP2152417B1 (fr) 2007-05-04 2018-07-11 Opko Diagnostics, LLC Dispositif et méthode pour analyses dans des systèmes microfluidiques
DE102007037788B4 (de) * 2007-08-10 2010-11-04 Leibniz-Institut für Analytische Wissenschaften-ISAS-e.V. Vorrichtung zum Ankoppeln von Leitungen an einen mikrofluidischen Chip
US20090121476A1 (en) * 2007-11-08 2009-05-14 The Government Of The Us, As Represented By The Secretary Of The Navy Microfluidic Bus for Interconnecting Multiple Fluid Conduits
EP2391451B1 (fr) 2009-02-02 2018-09-12 Opko Diagnostics, LLC Structures pour le contrôle des interactions de la lumière avec des dispositifs microfluidiques
DE102009053285B4 (de) 2009-11-13 2012-10-04 Karlsruher Institut für Technologie Verfahren zum reversiblen, parallelen Schließen einer Vielzahl von fluidischen Zuleitungen mit einem mikrofluidischen System
DE102010037532A1 (de) * 2010-09-14 2012-03-15 Andreas Hettich Gmbh & Co. Kg Anschlussvorrichtung zur fluidischen Kontaktierung von Mikrofluidikchips
GB2509440B (en) * 2011-10-25 2015-06-03 Ibm Microfluidic device with interconnects
PL2823427T3 (pl) 2012-03-05 2021-07-12 Oy Arctic Partners Ab Systemy komputerowe, sposoby i odczytywalne komputerowo nośniki danych do przewidywania ryzyka objętości gruczołu krokowego
US9791080B2 (en) * 2012-03-12 2017-10-17 Idex Health & Science Llc Microfluidic interconnect
US20150137015A1 (en) * 2012-07-12 2015-05-21 Agency For Science, Technology And Research Connector for microfluidic device, a method for injecting fluid into microfluidic device using the connector and a method of providing and operating a valve
CN112169851B (zh) * 2020-10-13 2022-03-29 中国科学院微电子研究所 一种微流道入口盖板及其制备和使用方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2812959A (en) * 1953-08-21 1957-11-12 Westinghouse Air Brake Co Flange union fitting with gland expandible into an opening
US3973792A (en) * 1974-01-14 1976-08-10 Bodenseewerk Perkin-Elmer & Co. Gmbh Connecting device for connecting chromatographic separating columns of glass
US4995646A (en) * 1989-11-28 1991-02-26 Crawford Fitting Company Connector for multiple lines
US6056331A (en) * 1996-09-12 2000-05-02 The Regents Of The University Of California Zero dead volume tube to surface seal
US5961932A (en) * 1997-06-20 1999-10-05 Eastman Kodak Company Reaction chamber for an integrated micro-ceramic chemical plant
US6209928B1 (en) * 1998-06-04 2001-04-03 The Regents Of The University Of California Microfluidic interconnects
US6077015A (en) * 1999-02-10 2000-06-20 Xerox Corporation Quick insertion screw
US6273478B1 (en) * 1999-03-30 2001-08-14 The Regents Of The University Of California Microfluidic interconnects
US6267143B1 (en) * 1999-06-29 2001-07-31 Upchurch Scientific, Inc. Selection valve with ferrule cluster

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO03081113A1 *

Also Published As

Publication number Publication date
AU2003212378A1 (en) 2003-10-08
DE10213272A1 (de) 2003-10-23
WO2003081113A1 (fr) 2003-10-02
US7475916B2 (en) 2009-01-13
US20060012167A1 (en) 2006-01-19

Similar Documents

Publication Publication Date Title
EP1480749B1 (fr) Systeme microfluidique
WO2003081113A1 (fr) Dispositif et procede de raccordement de conduites a des systemes microfluidiques
DE19947495C2 (de) Mikrofluidischer Mikrochip
EP1160573A2 (fr) Plaque de microtitrage et dispositif de pipetage multivoies couplé à celui
DE10319045A1 (de) Vorrichtung und Verfahren zur Aufbereitung Biopolymerhaltiger Flüssigkeiten
EP1750155B1 (fr) Procédé de fabrication d'une chambre d'échantillon
EP1599287A1 (fr) Procede et dispositif pour etablir un contact avec une structure microfluidique
DE10209897A1 (de) Mikrokomponenten-Anschlusssystem
EP1489404A1 (fr) Méthode de production d'une cellule d'écoulement 3-D pour microscope
EP2616179A1 (fr) Dispositif de raccordement pour la mise en contact fluidique de puces microfluidiques
DE10244960B4 (de) Kartusche mit Verbindung für einen Pump-Antrieb und Handhabungs-System für einen flüssigen oder gasförmigen Stoff
LU102325B1 (de) Probenbehältnis für eine Dispensiervorrichtung
DE102007054043B4 (de) Modulare mikrofluidische Funktionsplattform und deren Verwendung
EP1622715A1 (fr) Module microminiaturise
EP1340543A1 (fr) Système microfluidique
DE102004058828B4 (de) Vorrichtung und Verfahren zur parallelen Aufbereitung von Biopolymeren
DE102021204570A1 (de) Dosierkopf und Fluidiksystem zur Aufnahme und Dosierung wenigstens eines Mediums
DE102021116887A1 (de) Anordnung zum Generieren von Fluidsequenzen in einem Multifluidtransportkanal zum Konditionieren und Detektieren der in dem Multifluidtransportkanal generierten Fluidsequenzen
DE102022102894A1 (de) Mikrofluidiksystem und Verfahren zum Herstellen eines solchen
DE102021204571A1 (de) Dosierkopf und Dosiersystem zur Aufnahme und Dosierung eines Mediums
DE102022211313A1 (de) Dosiermodul mit Betätigungsfenster
EP1314479A2 (fr) Dispositif pour le transfert d' échantillons liquides
DE202004006024U1 (de) Flusskammer im Well
DE102017205286A1 (de) Mikrofluidische Vorrichtung und Verfahren zur Temperaturregelung eines Arbeitsfluids
EP1325258A1 (fr) Accouplement de fermeture miniature

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20040915

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT RO SE SI SK TR

AX Request for extension of the european patent

Extension state: AL LT LV MK

RIN1 Information on inventor provided before grant (corrected)

Inventor name: REICHEL, MARTIN

Inventor name: HUMMEL, STEFAN

Inventor name: SHIRLEY, STEPHEN

Inventor name: PFENNIG, ANNETTE

Inventor name: MUELLER, TORSTEN

RAP1 Party data changed (applicant data changed or rights of an application transferred)

Owner name: EVOTEC TECHNOLOGIES GMBH

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20101001