EP1145008A2 - Verfahren und vorrichtung zur diagnose allergischer symptome - Google Patents
Verfahren und vorrichtung zur diagnose allergischer symptomeInfo
- Publication number
- EP1145008A2 EP1145008A2 EP00904801A EP00904801A EP1145008A2 EP 1145008 A2 EP1145008 A2 EP 1145008A2 EP 00904801 A EP00904801 A EP 00904801A EP 00904801 A EP00904801 A EP 00904801A EP 1145008 A2 EP1145008 A2 EP 1145008A2
- Authority
- EP
- European Patent Office
- Prior art keywords
- antibodies
- binding protein
- protein
- carrier
- patient
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54386—Analytical elements
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
- B01J2219/00612—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports the surface being inorganic
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
- B01J2219/00614—Delimitation of the attachment areas
- B01J2219/00617—Delimitation of the attachment areas by chemical means
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
- B01J2219/00623—Immobilisation or binding
- B01J2219/0063—Other, e.g. van der Waals forces, hydrogen bonding
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
- B01J2219/00632—Introduction of reactive groups to the surface
- B01J2219/00637—Introduction of reactive groups to the surface by coating it with another layer
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00659—Two-dimensional arrays
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00718—Type of compounds synthesised
- B01J2219/0072—Organic compounds
Definitions
- the carrier is designed as a reflective solid carrier on which the binding protein is applied in a monomolecular, surface-active layer, and that after an incubation period, an immunoreaction between the antibody and the antigen caused increase in layer thickness of the protein-antibody layer is determined by an ellipsometric measurement method.
- the binding protein in a desired monomolecular surface-active layer.
- the monomolecular layer ensures that minimal substances can be used and detected.
- IgG immunoglobulin E antibodies
- IgE immunoglobulin E antibodies
- the antibody (IgG) - like a "Y" - consists of three areas. The Fab regions, corresponding to the poor, are responsible for the immune-specific responsiveness, while the Fc fragment, the foot end, reacts unspecifically.
- the IgG is coupled to the binding protein, for example protein A, via the Fc fragment.
- the carrier is scanned on a two-coordinate measuring table of an egg lipometer in a software-controlled manner by the laser beam of the egg lipometer. The result of the scanning process is then evaluated using an evaluation software.
- FIG. 2 shows a plan view of the carrier from FIG. 1 with a schematic illustration of a micropipette arranged in a row
- FIG. 3 a basic illustration, not to scale, of a side view of a carrier
- Figure 5 a simplified flow diagram of a method for the detection of antigen.
- a device for diagnosing allergic symptoms essentially consists of a sensor base 1 to which patient-specific allergens 2 can be connected.
- the carrier 3 is designed as a rectangular silicon plate on which a monomolecular layer of a binding protein 4 is arranged using the Langmuir-Blodgett technique (coating of monomolecular films of surface-active substances by means of a special dipping process).
- Protein A PrA
- protein G protein
- the binding protein 4 is arranged in a matrix in islands 8 on the Si plate.
- the carrier 3 has an exposed edge 6.
- the released edge 6 serves on the one hand as an attack surface for holders and, on the other hand, optionally as a reference surface for determining the refractive index of the carrier 3.
- the islands 8 are equipped with patient-specific antibodies 5. Different islands 8 have antibodies 5, each with a different specificity.
- Isotypic immunoglobulin G antibodies (IgG) separated from patient serum are used as antibody 5.
- immunoglobulin E antibodies IgE.
- the substance of the islands 8, on which an immune reaction has taken place is broken down into its atomic components.
- the separated elementary fractions achieved are reduced by the known binding protein and antibody proportions.
- the residual substance is the allergy-causing factor that is being sought and is evaluated in an allergen database or software library.
- This allergen database is a collection of all known allergens with the distribution of their elementary components, their biochemical structure as well as molecular structural elements and all conditions necessary for the evaluation.
- the separator result is reconstructed with the help of the allergen database using an integrated processing program and identified by comparisons and restrictions.
Abstract
Description
Claims
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE1999100119 DE19900119C2 (de) | 1999-01-06 | 1999-01-06 | Verfahren und Vorrichtung zur Diagnose allergischer Symptome |
DE19900119 | 1999-01-06 | ||
PCT/DE2000/000001 WO2000040967A2 (de) | 1999-01-06 | 2000-01-03 | Verfahren und vorrichtung zur diagnose allergischer symptome |
Publications (1)
Publication Number | Publication Date |
---|---|
EP1145008A2 true EP1145008A2 (de) | 2001-10-17 |
Family
ID=7893600
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP00904801A Withdrawn EP1145008A2 (de) | 1999-01-06 | 2000-01-03 | Verfahren und vorrichtung zur diagnose allergischer symptome |
Country Status (4)
Country | Link |
---|---|
EP (1) | EP1145008A2 (de) |
JP (1) | JP2002534682A (de) |
DE (1) | DE19900119C2 (de) |
WO (1) | WO2000040967A2 (de) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE10120562C2 (de) * | 2001-04-26 | 2003-04-10 | Horst Messer | Verfahren zur Diagnose von übertragbaren spongiformen Enzephalopathien |
EP1564556A1 (de) | 2004-02-17 | 2005-08-17 | DST Diagnostic Science & Technology GmbH | Verfahren und Vorrichtung zur Bestimmung mehrerer Analyten mit simultaner interner Kontrolle |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3042535C2 (de) * | 1980-07-02 | 1986-08-21 | Wang, Wei-Kung, T'ai-pei | Vorrichtung zur Feststellung von Fluoreszenz |
DE3176524D1 (en) * | 1981-06-22 | 1987-12-17 | Battelle Memorial Institute | A method for determining bioactive substances |
SE468157B (sv) * | 1991-03-22 | 1992-11-16 | Goeteborgs Analyslab | Foerfarande foer att bringa tvaa eller flera komponenter i intim kontakt med varandra samt foer genomfoerande av foerfarandet avsedd anordning, vilken omfattar en flexibel slang |
US5552272A (en) * | 1993-06-10 | 1996-09-03 | Biostar, Inc. | Detection of an analyte by fluorescence using a thin film optical device |
US5633724A (en) * | 1995-08-29 | 1997-05-27 | Hewlett-Packard Company | Evanescent scanning of biochemical array |
SE9602545L (sv) * | 1996-06-25 | 1997-12-26 | Michael Mecklenburg | Metod för att diskriminera komplexa biologiska prover |
DE19629243A1 (de) * | 1996-07-19 | 1998-01-29 | Udo Dr Ris | Verfahren zur quantitativen und/oder qualitativen Bestimmung von Atomen oder Molekülen |
US5812272A (en) * | 1997-01-30 | 1998-09-22 | Hewlett-Packard Company | Apparatus and method with tiled light source array for integrated assay sensing |
EP0886141A1 (de) * | 1997-06-23 | 1998-12-23 | C.S.E.M. Centre Suisse D'electronique Et De Microtechnique Sa | Optische Sensorvorrichtung und Verfahren zur hochempfindlichen Detektion von chemischen oder biochemischen Analyten |
-
1999
- 1999-01-06 DE DE1999100119 patent/DE19900119C2/de not_active Expired - Fee Related
-
2000
- 2000-01-03 EP EP00904801A patent/EP1145008A2/de not_active Withdrawn
- 2000-01-03 JP JP2000592635A patent/JP2002534682A/ja not_active Withdrawn
- 2000-01-03 WO PCT/DE2000/000001 patent/WO2000040967A2/de not_active Application Discontinuation
Non-Patent Citations (1)
Title |
---|
See references of WO0040967A3 * |
Also Published As
Publication number | Publication date |
---|---|
WO2000040967A3 (de) | 2001-10-25 |
DE19900119C2 (de) | 2001-02-22 |
WO2000040967A2 (de) | 2000-07-13 |
JP2002534682A (ja) | 2002-10-15 |
DE19900119A1 (de) | 2000-08-17 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
DE69826583T2 (de) | Vorrichtung und Verfahren zum Erreichen von klinischen wichtigen Analytverhältnissen | |
DE2536572C2 (de) | Diagnostisches Verfahren zum Nachweisen der An- oder Abwesenheit eines ausgewählten Antigens oder Antikörpers in einer biologischen Flüssigkeitsprobe | |
DE69829089T2 (de) | Vorrichtung und Apparat zur gleichzeitigen Detektion von mehreren Analyten | |
DE2436010C2 (de) | ||
DE2618386C2 (de) | Diagnostisches Verfahren zum Bestimmen der Anwesenheit oder Abwesenheit nachzuweisender biologischer Teilchen | |
EP1143248B1 (de) | Verfahren zu Immobilisierung von Konjugaten in diagnostischen Tests | |
EP0126450B1 (de) | Partikel sowie Verfahren zum Nachweis von Antigenen und/oder Antikörpern unter Verwendung der Partikel | |
EP1759209B1 (de) | Verfahren zur erhöhung des dynamischen messbereichs von auf spezifischen bindereaktionen basierenden, insbesondere immunologischen testelementen | |
EP0998675B1 (de) | Verwendung von kontrollflächen zur detektion von störproben in einem nachweisverfahren | |
EP0098590B1 (de) | Immunchemisches Messverfahren | |
DE60117699T2 (de) | Analyseverfahren durch spezifisches binden und vorrichtung, die das verfahren einsetzt | |
DE2650106C2 (de) | ||
EP1536232B1 (de) | Analytischer Sandwichtest zur Bestimmung von NT-proBNP | |
DE60116889T2 (de) | Test zum direkten nachweis eines inflammatorischen anzeigers ein einer körperflüssigkeitsprobe | |
EP0407904B1 (de) | Verfahren zur Bestimmung eines Analyten | |
DE3135196A1 (de) | Verfahren, mittel und vorrichtung zur bestimmung biologischer komponenten | |
EP0469377A2 (de) | Analysesystem und Verfahren zur Bestimmung eines Analyten in einer fluiden Probe | |
DE60212776T2 (de) | Spezifisches Bindungsanalyseverfahren | |
WO2000042434A1 (de) | Verfahren und vorrichtung zur bestimmung eines analyten | |
DE69732034T2 (de) | Assay-verfahren | |
EP0379216B1 (de) | Verfahren und Reagenz zur Bestimmung von immunologisch nachweisbaren Substanzen | |
AT395076B (de) | Vorrichtung und verfahren fuer die diagnostische bestimmung klinischer parameter | |
DE69819833T2 (de) | Verfahren und Teststreife zur Veringerung des Harnstoffeinflusses bei immunochromatographischen Messungen unter Verwendung von Urinproben | |
DE69817329T2 (de) | Vorrichtung und Verfahren zur Identifikation einer Substanz durch Oberflächenwechselwirkungen | |
DE2440367A1 (de) | Verfahren zum ausfuehren von immunologischen oberflaechenuntersuchungen |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
|
17P | Request for examination filed |
Effective date: 20010804 |
|
AK | Designated contracting states |
Kind code of ref document: A2 Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE |
|
XX | Miscellaneous (additional remarks) |
Free format text: DERZEIT SIND DIE WIPO-PUBLIKATIONSDATEN A3 NICHT VERFUEGBAR. |
|
PUAK | Availability of information related to the publication of the international search report |
Free format text: ORIGINAL CODE: 0009015 |
|
AK | Designated contracting states |
Kind code of ref document: A3 Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE |
|
GRAP | Despatch of communication of intention to grant a patent |
Free format text: ORIGINAL CODE: EPIDOSNIGR1 |
|
STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN |
|
18D | Application deemed to be withdrawn |
Effective date: 20031209 |