EP1134231B1 - Domaines variables de la chaine lourde d'anticorps contre des lipases humaines alimentaires et leurs utilisations - Google Patents

Domaines variables de la chaine lourde d'anticorps contre des lipases humaines alimentaires et leurs utilisations Download PDF

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EP1134231B1
EP1134231B1 EP01200703A EP01200703A EP1134231B1 EP 1134231 B1 EP1134231 B1 EP 1134231B1 EP 01200703 A EP01200703 A EP 01200703A EP 01200703 A EP01200703 A EP 01200703A EP 1134231 B1 EP1134231 B1 EP 1134231B1
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antibody
fragment
hpl
human
fragments
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EP1134231A1 (fr
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Sandra Bezemer
Monique Van De Burg
Johannes Joseph Wilhelmus De Haard
Erwin Tareilus
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Unilever PLC
Unilever NV
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/505—Medicinal preparations containing antigens or antibodies comprising antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/22—Immunoglobulins specific features characterized by taxonomic origin from camelids, e.g. camel, llama or dromedary

Definitions

  • the present invention is in the field of applied biotechnology and relates in particular to the inhibition of human lipase.
  • Cardiovascular diseases are the number one cause of death in the Western World. Epidemeologic and experimental data show clearly that high serum cholesterol levels, more precise high level of Low Density Lipoprotein particles, which contain cholesterol show a strong correlation with the occurrence of cardiovascular diseases. It is also well known that foods products containing fats high in saturated fatty acids contribute to high serum Low Density Lipoprotein levels. It has also be stated that hydrolysis of dietary fats, thereby liberating fatty acids in the stomach and intestinal tract increases the adsorption of cholesterol by the epithelial cells of the intestinal tract and consequently hydrolysis of dietary fats contribute to increase of the serum Low Density Lipoprotein levels. Several human dietary enzymes are involved in this hydrolysis reaction. A further reason to reduce the hydrolysis of dietary fats and the subsequent liberation of fatty acids is to prevent or to reduce an increase of body weight or event to reduce the body weight.
  • enzymes in the gastrointestinal tract may be involved in undesirable physiological reactions.
  • human dietary enzymes include oxidoreductases, transferases, hydrolases (e.g. lipases, proteolytic enzymes and ureases), lyases, isomerases and ligases or synthetases.
  • WO 98/34630 describes the use of a gastrointestinal lipase inhibitor for the manufacture of oral medicaments for treating or preventing type II diabetes mellitus.
  • a preferred gastrointestinal lipase inhibitor is tetrahydrolipstatin.
  • WO 97/49805 relates to recognition molecules that interact specifically with the active site or cleft of a target molecule such as an enzyme. It discloses antibodies naturally devoid of light chains, and VHH fractions thereof, directed against pancreatic alpha-amylases and capable of inhibiting the enzymatic activity of said amylases. Therapeutic compositions comprising said antibodies are also disclosed.
  • WO 99/46300 describes the use of VHHs in the preparation of products, for example food products. VHHs are said to be more stable under destabilizing conditions than traditional antibodies.
  • Aoubala et al in The Journal of Biological Chemistry, 8, 1995 pp 3932-3937 discloses monoclonal antibodies against human pancreatic lipase. Also Bezzine et al in Biochemistry (1998), 11846-11855 describes the binding of monoclonal antibodies to human pancreatic lipase. However a problem with monoclonal antibodies is that they are expensive, difficult to prepare and are generally not stable under the conditions in the human tract.
  • V H Hs a special class of antibodies or fragments thereof namely those which are naturally free of light chains and commonly referred to as V H Hs can be used for the inhibition or modulation of human dietary enzymes.
  • the present invention relates to an antibody, or fragment thereof, capable of binding specifically to one or more human dietary lipases, said antibody or fragment thereof comprising a heavy chain variable domain derived from an immunoglobulin naturally devoid of light chains.
  • the invention relates to a food product or a pharmaceutical product comprising an antibody, or fragment thereof, capable of binding specifically to one or more human dietary lipases, said antibody or fragment thereof comprising a heavy chain variable domain derived from an immunoglobulin naturally devoid of light chains.
  • the invention relates to the use of an antibody, or fragment thereof, capable of binding specifically to one or more human dietary lipases, said antibody or fragment thereof comprising a heavy chain variable domain derived from an immunoglobulin naturally devoid of light chains, in the preparation of a medicament or food for inhibiting the activity of one or more human dietary lipases in the human body.
  • the invention relates to the use of an antibody, or fragment thereof, capable of binding specifically to one or more human dietary lipases, said antibody or fragment thereof comprising a heavy chain variable domain derived from an immunoglobulin naturally devoid of light chains, for the cosmetic control of body weight of human beings.
  • V H H refers to the single heavy chain variable domain antibodies of the type that can be found in Camelid mammals which are naturally devoid of light chains; synthetic V H H can be construed accordingly.
  • antibodies refers to immunoglobulins which may be derived from natural sources or may be synthetically produced, in whole or as antibody fragment.
  • antibody fragment is a portion of a whole antibody which retains the ability to exhibit antigen binding activity. Functionalized antibody fragments are also embraced in this term.
  • the term "functionalized antibody fragment” is used for indicating an antibody or fragment thereof to which one or more functional groups, including enzymes and other binding polypeptides, are attached resulting in fusion products of such antibody fragment with another biofunctional molecule.
  • human dietary enzymes is used for enzymes which may be present and are physiologically active in the gastro-intestinal tract e.g. under stomach conditions or under intestinal conditions.
  • Antibodies are protein molecules belonging to a group of immunoglobulins generated by the immune system in response to an antigen.
  • the structure of most antibody molecules is based on a unit comprising four polypeptides, two identical heavy chains and two identical light chains, which are covalently linked by disulphide bonds. Each of these chains is folded in discrete domains.
  • the carboxy-terminal regions of both heavy and light chains are conserved in sequence and are called the constant regions, comprising one or more so-called C-domains.
  • the amino-terminal regions of the heavy and light chains also known as variable (V) domains, are variable in sequence and determine the specificity of the antibody.
  • V L and V H The regions in the variable domains of the light and heavy chains (V L and V H respectively) responsible for antigen binding activity are known as the hypervariable or complementarity determining regions (CDR), while the framework regions (FR) are responsible for the typical immunoglobulin fold of the V-region.
  • CDR hypervariable or complementarity determining regions
  • FR framework regions
  • Natural antibodies generally have at least two identical antigen-binding sites defined by the association of the heavy and light chain variable regions. Generally most naturally occurring antibodies need both a V H and V L to form a complete antigen binding site and retain full immunoreactivity.
  • immunoglobulins capable of exhibiting the functional properties of the four-chain immunoglobulins described above but which comprise two heavy polypeptide chains and which furthermore are devoid of light polypeptide chains have been described in WO 94/04678 .
  • Methods for the preparation of such antibodies or fragments thereof on a large scale comprising the transformation of a mould or yeast with an expressible DNA sequence encoding the antibody or fragment are described in WO 94/25591 .
  • immunoglobulins described in WO 94/4678 which may be isolated from the serum of Camelids, do not rely upon the association of heavy and light chain variable domains for the formation of the antigen-binding site but instead the heavy polypeptide chains alone naturally form the complete antigen binding site.
  • These immunoglobulins hereinafter referred to as "heavy-chain immunoglobulins" (or V H H) are thus quite distinct from the heavy chains obtained by the degradation of common (four-chain) immunoglobulins or by direct cloning which thereby contain only a part of the antigen-binding site and require a light chain partner for the formation of a complete antigen binding site in order to obtain optimal antigen-binding characteristics.
  • V H H's are capable of inhibiting human dietary enzymes, in particular enzymes involved in the hydrolysis of dietary fats, and thereby reduce the absorption of free fatty acids effectively.
  • V H H's are used for the inhibition of human enzymes involved in the hydrolysis of dietary fats, examples of these enzymes are Human Pancreatic Lipase and Human Gastric Lipase.
  • HPL Human Pancreatic Lipase
  • the enzyme is active at neutral pH in the small intestine, where it catalyses the hydrolysis of fatty acids in the sn-1 and sn-3 position of triacylglycerides.
  • the enzyme requires a cofactor called colipase for lipolytic action on duodenal fats.
  • the structure of HPL consists of an amino-terminal domain (residues 1 through 336) and a carboxy-terminal domain (residues 337 through 448) that is involved in binding colipase.
  • HGL Human Gastric Lipase belongs to the family of the acid lipase family, which refers to its stability and activity in the highly acidic environment of the stomach. HGL is responsible for the hydrolysis of 17.5% of the meal triacylglyceride.
  • the crystal structure of the enzyme which contains 379 amino acid residues, reveals the presence of a core domain typical for the alpha/beta hydrolase family and a "cap" domain, similar to what has been found in Serine carboxypeptidases.
  • a preferred embodiment of the present invention involves the partial inhibition of human dietary lipases using V H H's.
  • the enzymes are only partially inhibited to ensure that no deficiences of important ingredients will occur.
  • the level of inhibition, measured in accordance to Figure 3 is between 2 to 90%, more preferred 3-30%, most preferred 5-20%.
  • antibodies can be used in their entirety (e.g. in a form which is equal to or closely resembles the natural form in the Camelid source).
  • fragments of these antibodies e.g. V H H's may be used. If fragments are used then it is preferred that these fragments comprise one or more sequences which are equal to or closely resemble the CDR regions in the natural V H H's. Particularly preferably these fragments comprise a sequence which is equal to or closely resembles the CDR3 region of a natural V H H.
  • V H H's (including either entire V H H's or fragments thereof) according to the present invention are characterised by a CDR3 selected from the following classes:
  • V H H's of the first class are HGL#1 and HGL#16.
  • Preferred examples of V H H's of the second class are HGL#4 and HGL#10.
  • Preferred examples of V H H's of the third class are HGL#8 and HGL#9.
  • a preferred example of V H H's of the fourth class is HGL#11.
  • V H H's according to the present invention are chacterised by a CDR3 selected from the following classes:
  • V H H of class (a) is HPL#12, HPL#14 and HPL#30
  • An example of a V H H of class (b) is HPL#19
  • An example of a V H H of class (c) is HPL#18
  • An example of a V H H of class (d) is HPL#13
  • An example of a V H H of class (e) is HPL#11
  • An example of a V H H of class (f) is HPL#22
  • An example of a V H H of class (g) is HPL#15
  • An example of a V H H of class (h) is HPL#17
  • V H H's in accordance to the present invention can be used for the inhibition of the activity of human dietary lipases. Surprisingly it has been found that the V H H's are often more stable than traditional antibodies under conditions similar to those in the gastric intestinal tract. In particular preferred V H H's in accordance to the invention have a stability (as measured in example 4.3) of at least 75% after 1 hour.
  • V H H's in accordance to the present invention can be administered to human beings in any desirable form.
  • the V H H's can be used in pharmaceutical compositions. These compositions normally comprise in addition to the V H H's a suitable carrier material.
  • the V H H's can be incorporated into medicines for oral use such as tablets, capsules, medicinal liquors, powders, but other application forms e.g. as an injection, topical applications etc may equally be suitable.
  • the V H H's can be used in food products.
  • suitable food products are margarines and other bread spreads, dressings, beverages including fruit juices and tea and coffee, bakery products such as cookies, biscuits, bread, pizza etc, sauces including hot or cold sauces, frozen confectionery materials e.f water-ice or ice-cream, dairy products e.g. desserts, yoghurt, cheese etc, cereal products, for example breakfast cereals, sweets such as pastilles, lollypops, bars, chocolate etc.
  • a suitable intake per meal of antibodies could be such that the molar ratio of antibody to the relevant dietary enzyme is between 10 : 1 and 1 : 10. It is well within the ability of the skilled person to adapt the concentration of antibodies in the product such that these amounts are consumed.
  • the invention is applicable to the use of any immunoglobulin variable domain, which forms a complete antigen binding site.
  • the immunoglobulin may be derived from natural sources or synthetically produced.
  • the invention relates to the use of heavy chain variable domains derived from an immunoglobulin devoid of light chains, most suitably from an immunoglobulin naturally devoid of light chains such as are obtainable from lymphoid cells, especially peripheral blood lymphocytes, bone marrow cells or spleen cells derived from Camelids as described in WO 94/04678 (Casterman et al ).
  • Another benefit of single domain binding units is that these molecules can readily and conveniently be produced economically on a large scale, for example using a transformed lower eukaryotic host as described in WO 94/25591 (Unilever). This describes a production system that delivers high amounts of secreted antibody fragments with a low degree of impurities present in the secreted fraction, thereby enabling simple down stream processing procedures for purification.
  • the invention also provides host cells and expression vectors enabling high levels of production and secretion of the binding proteins.
  • Heavy chain variable domains derived from an immunoglobulin naturally devoid of light chains having a determined antigen specificity may conveniently be obtained by screening expression libraries of cloned fragments of genes encoding Camelid immunoglobulins generated using conventional techniques, as described, for example, in EP-A-0584421 and Example 1.
  • Preferred methods to enrich for binding domains recognising the human dietary engyme, thereby limiting the numbers of clones, which have to be screened for the identification of inhibiting fragments are yeast display ( WO 94/01567 from Unilever) or phage display.
  • Enzyme inhibiting antigen binding proteins may be prepared by transforming a host by incorporating a gene encoding the polypeptide as set forth above and expressing said gene in said host.
  • the host or hosts may be selected from prokaryotic bacteria, such as Gram-negative bacteria, for example Escherichia coli , and Gram-positive bacteria, for example Bacillus subtilis and in particular lactic acid bacteria, lower eukaryotes such as yeasts, for example belonging to the genera Saccharomyces, Kluyveromyces , Hansenula or Pichia , or moulds such as those belonging to the genera Aspergillus or Trichoderma .
  • prokaryotic bacteria such as Gram-negative bacteria, for example Escherichia coli
  • Gram-positive bacteria for example Bacillus subtilis and in particular lactic acid bacteria
  • lower eukaryotes such as yeasts, for example belonging to the genera Saccharomyces, Kluyveromyces , Hansenula or Pichia
  • moulds such as those belonging to the genera Aspergillus or Trichoderma .
  • Preferred hosts for use in connection with the present invention are the lower eukaryotic moulds and yeasts.
  • Proteins for use according to the invention may be recovered and purified using conventional techniques such as affinity chromatography, ion exchange chromatography or gel filtration chromatography.
  • binding activity of the binding proteins prepared according to the invention may conveniently be measured by standard techniques known in the art such as enzyme-linked immunoadsorbant assay (ELISA), radioimmune assay (RIA) or with biosensors.
  • ELISA enzyme-linked immunoadsorbant assay
  • RIA radioimmune assay
  • EXAMPLE 1 Induction of a humoral immune response in llama.
  • HPL Human Pancreatic Lipase
  • a llama was immunised with an HPL in oil emulsion obtained by mixing 2 ml antigen in water and 3 ml Specol, ( Bokhout, B.A., Van Gaalen, C. & Van der Heijden, P.J. (1981) Vet. Immunol. Immunopath. 2, 491-500 .
  • Biotinylated HPL was captured by the coated streptavidin during 16 hours at 4°C from a solution with a concentration of 2.5 ⁇ g/ml enzyme in PBST, followed by washing of the plate with PBTS to remove free biotinylated HPL.
  • Serum samples were tested in serial dilutions (in 2% skimmed milk solution in PBST). Subsequently the bound llama antibodies were detected with polyclonal rabbit-anti-llama antiserum (obtained via immunising rabbits with llama immunoglobulins purified via Protein A and Protein G columns ( Hamers-Casterman, C., Atarhouch, T., Muyldermans, S., Robinson, G., Hamers, C., Songa, E.B., Bendahman, N. & Hamers, R. (1993).
  • the titre of inhibiting antibodies was determined in the LIPASE-PS assay (Sigma Diagnostics), in which the enzymatic hydrolysis of 1,2-diglyceride into 2-monoglyceride and fatty acid can be measured kinetically in a spectrophotometer at a wavelength of 550 nm.
  • LIPASE-PS assay Sigma Diagnostics
  • 5 ⁇ l (diluted) serum was mixed with 10 ⁇ l distilled water and 5 ⁇ l HPL (approximately 250 lipase units/ml. From this mixture 5 ⁇ l was added to 150 ⁇ l substrate solution (LIPASE-PS Substrate Reagent) using the wells of a microtiter plate as reaction vessels.
  • Activator solution 50 ⁇ l/well was added and colour development was measured kinetically during a period of 10 minutes at 550 nm and 37°C, whereby a change in colour intensity implied enzymatic activity.
  • EXAMPLE 2 Cloning, selection and screening of clones producing llama V H H fragments inhibiting Human Pancreatic Lipase.
  • Llama RNA was isolated from its lymphocytes using a blood sample taken 8 weeks after the first immunisation. At that point in time the llama had the highest titre of HPL recognising antibodies as measured in ELISA.
  • RNA was isolated by guanidium thiocyanate extraction according to Chomczynski, P. & Sacchi, N. (1987). Anal. Biochem. 162(1), 156-159 .
  • DNA fragments encoding V H H and part of the long or short hinge region were amplified by PCR using three specific primers as described in example II.2.1 of WO99/46300 .
  • the DNA-fragments generated by PCR were digested with Pst I (coinciding with codon 4 and 5 of the V H H domain, encoding the amino acids L-Q) and NotI (introduced at the 5' end of the hinge specific oligonucleotide primers, coinciding with the amino acid sequence A-A-A).
  • the digested PCR products were cloned in the phagemid vector pUR5071 ( figure 4 ) as gene-fragments encoding the V H H domain including the hinge region fused to the gene III protein of the E . coli bacteriophage M13.
  • Phage particles exposing V H H fragments were prepared by infection of E . coli cells harbouring the phagemid with helperphage VCS-M13 according to Marks, J.D et.al (1991) J. Mol. Biol. 222, 581-597 .
  • the carboxy terminal domain was prepared by proteolysis with chymotrypsin and purified with reversed phase HPLC. Lipase was biotinylated and used for selection of the immune library (combined short hinge and long hinge) at 15 and 70 nM during round one and at 1 and 3 and 15 nM during round two. During the binding phase of the selection "application conditions" (inclusion of 5.3 mM cholic acid and 36 mM deoxycholate) were used. Phage particles bound via their displayed antibody fragments to the biotinylated lipase or the carboxy terminal domain peptide were pulled out of the solution with streptavidin coated magnetic beads (Dynal) (see ( Hawkins, T, DNA Seq. 1992; 3(2) 65-9 ). After washing, phage was eluted with triethylamine.
  • V H H fragments Individual E . coli clones obtained after two rounds of selection were grown in wells of microtiter plates, and the production of V H H fragments was induced by the addition of 0.1 mM isopropyl- ⁇ -D-thiogalactopyranoside. After 16 hours of growth, the culture supernatant of the clones was analysed in ELISA for the presence of V H H fragments, which specifically bind to indirectly immobilised biotinylated HPL, using a streptavidin coated plate as a negative control.
  • Bound V H H fragments were detected with rabbit anti-llama V H H polyclonal antibodies followed by incubation with goat anti-rabbit polyclonal antibodies conjugated to horse radish peroxidase (BIORAD), or with mouse monoclonal anti-myc antibody ATCC myc 1-9E 10-2 followed by incubation with polyclonal rabbit anti-mouse conjugated to horse radish peroxidase (DAKO).
  • BIORAD goat anti-rabbit polyclonal antibodies conjugated to horse radish peroxidase
  • DAKO polyclonal rabbit anti-mouse conjugated to horse radish peroxidase
  • the myc-tag is encoded in the phage display vector, which results in the addition of this peptide sequence to the carboxy terminus of the V H H fragments.
  • the lipase inhibiting capacity of the V H H fragments was demonstrated in an enzyme activity assay (Sigma).
  • Different anti-HPL clones were identified by their characteristic HinFI fingerprint pattern (Marks et al., as above), for which the V H H encoding insert was amplified with the M13REV- and the gene III-primer.
  • the resulting PCR-product was digested with the restriction enzyme HinFI, whose recognition site frequently occurs within antibody genes.
  • the representative clones were grown on 5 ml scale and the cells were harvested after a relative short induction time of 3.5 hours at 37°C.
  • the antibodies can be grouped in three classes, as is shown in the following amino acid sequences, wherein the resepective CDR regions are indicated in bold CDR1 being the first bold strand etc.
  • HPL#12, HPL#18 and HPL#19 are characterised by a CDR3 region having a length of 13 amino acids
  • HPL#11, HPL#13 and HPL#22 are characterised by a CDR3 region of 12 amino acids
  • HPL#15 is characterised by a CDR3 region of 14 amino acids.
  • EXAMPLE 3 The efficacy of V H H fragments to inhibit Human Pancreatic Lipase.
  • V H H encoding sequences of clones HPL#11, HPL#13, HPL#15, HPL#17, HPL#18 and HPL#19 were digested with PstI and Bst EII from the E . coli phagemid vectors pUR5084, pUR5082, pUR5095, pUR5080, pUR5086 and pUR5087 respectively, and cloned in the episomal S .
  • V H H fragments were obtained at the CBS, Baarn, The Netherlands as CBS100012 for the secretion of V H H fragments, thereby obtaining pUR5091, pUR5090, pUR1403, pUR5088, pUR5092 and pUR5093 respectively.
  • Secretion of V H H fragments with carboxy terminal tag-sequences was accomplished by cloning in plasmid pUR4585, which is identical to plasmid pUR4547 except encoding the myc-tag for detection with monoclonal antibody myc 1 - 9E10.2 (ATCC) and the hexahistidine tail for purification with IMAC.
  • Plasmid constructs pUR5099, pUR5098, pUR5097, pUR5096, pUR5263 and pUR5264 were made encoding the tagged V H H fragments of HPL#11, HPL#13, HPL#15, HPL#17, HPL#18 and HPL#19 respectively.
  • Both parental plasmids pUR4547 and pUR4585 contain the GAL7 promoter for inducible expression of the V H H gene product, the selectable markers bla ( ⁇ -lactamase) to discriminate transformants in E . coli by resistance to the antibioticum ampicillin and Leu2d ( ⁇ -isopropylmalate dehydrogenase) for selection of transformed S . cerevisiae, and an E . coli origin of replication. Secretion is accomplished by fusing the SUC2 leader sequence to the amino terminus of the V H H product according to Harmsen, M.M. et al (1993) Gene 125, 115-123 .
  • Transformants in E . coli containing the S . cerevisiae shuttle constructs with the V H H genes were identified by PCR screening using primers M13REV and M13U for amplification of the V H H encoding insert and by restriction enzyme analysis on plasmid DNA. Plasmid DNA purified with the Quick-prep kit (Qiagen) was used for transformation of S. cerevisiae strain VWK18 gal1::URA3 , ura3,leu2 by the lithium acetate procedure as decribed by Gietz, R.D et al (1995) Yeast 11(4), 355-360 .
  • V H H gene expression both pre-cultures were diluted 1/10 in 1 ml of YPD medium (1% yeast extract, 2% peptone, 2% glucose, 2% galactose) for induction of the GAL7 promotor and grown during 48 hours at 30°C using 8 wells culture plates for cultivation.
  • the V H H production in the medium fraction of these clones was examined by analysis on a Coomassie blue stained polyacrylamide gel. Their functional characteristics were confirmed in ELISA on indirectly immobilised biotinylated HPL and in the lipase enzyme activity assay.
  • the S . cerevisiae transformants were induced in 250 ml shake flasks using 30 ml of culture medium as described in section 3.1. Following 48 hours of induction, the medium fraction was separated from the cells by centrifugation. For purification via immobilised metal affinity chromatography (IMAC) 12 ml of each medium fraction was adjusted to 50 mM NaH 2 PO4 (pH 8.0), 10 mM Tris-HCl (pH 8.0) and 100 mM NaCl and added to 1 ml of TALON column material(CLONTECH).
  • IMAC immobilised metal affinity chromatography
  • the hexahistidine tagged V H H fragments were bound to the immobilised metal ions in a batchwise fashion by rotating the column material head-over-head during 30 minutes; washing of the column material with sonication buffer and elution with 100 mM imidazol (Sigma) was performed according to the instructions of the supplier(CLONTECH). After removal of imidazol by dialysis against PBS, the amount of purified V H H was determined by measuring the optical density at 280 nm using the calculated molar extinction coefficient. Analysis on a Coommassie stained protein gel confirmed the purity and the measured amount of V H H. Between 100 and 500 ⁇ g antibody fragment was purified from 12 ml of culture.
  • Yeast comprising pUR5099, pUR5097, pUR5263 and pUR5264 encoding the V H H domains of HPL#11, HPL#15, HPL#18 and HPL#19 respectively were cultivated and induced in one litre of YPD medium.
  • the cells were removed by centrifugation and the medium fraction containing the antibody fragment was concentrated five-fold with a dialysis unit (Hemophan fiber dialyzer GFSplus 12, Gambro, Breda).
  • HPL#11, HPL#15 and HPL#18 were purified by affinity chromatography on protein A sepharose (Pharmacia).
  • HPL#19 which did not bind to protein A, was purified with IMAC, yielding 3.7 mg V H H per litre of culture. After dialysis against PBS, the factions can be used for inhibition experiments in intestinal juice.
  • EXAMPLE 4 Isolation of llama V H H fragments capable to inhibit Human Gastric Lipase.
  • a male llama was immunised with Human Gastric Lipase purified as described in Moreau, H et al (1992) J. Mol. Biol. 225(1), 147-153 according to the procedure indicated above and the titration of blood samples was performed on biotinylated enzyme in ELISA as described in example 1 ( Fig. 3A ).
  • the titre of inhibiting antibodies was determined in an enzyme assay, in which 1,2-0-dilauryl-rac-glycero-3-glutaric acid-resorufin ester (DGGR, Boehringer Mannheim) was used as chromogenic substrate.
  • DGGR 1,2-0-dilauryl-rac-glycero-3-glutaric acid-resorufin ester
  • 5 ⁇ l HGL solution 0.1 mg/ml
  • 10 ⁇ l of (diluted) serum sample in the well of a microtiter plate.
  • the reaction was started by the addition of 165 ⁇ l of buffer containing 100 mM MES pH6.0 and 0.6 M NaCl and 20 ⁇ l DGGR solution (1 mg/ml in dioxane/Thesit-solution (1:1)).
  • the kinetics of the enzymatic conversion was measured at 572 nm during a period of 30 minutes at 37 °C with a Spectramax spectrophotometer. With this assay the titre of enzyme inhibiting antibodies was determined in serum samples taken after different time intervals ( Fig. 3B ).
  • RNA was isolated from the lymphocytes of a blood sample taken 9 weeks after the start of the immunisation. Random primed cDNA was prepared and used for the amplification of short hinge and long hinge derived V H H fragments, which were cloned in the phagemid vector pUR5071. A short hinge derived library was constructed, which contains 5.4x10 7 clones and a long hinge derived library with 4.5x10 7 clones.
  • V H H encoding gene segments could be classified into four groups according to the length of their CDR3 (see below). The four groups are: HGL#1 and HGL#16; HGL#4 and HGL#10; HGL#8, HGL#9 and HGL#15; and HGL#11.
  • V H H encoding gene fragments were digested with Pst I and Bst EII from the phage display vector pUR5071.
  • the DNA fragments were cloned into the episomal S . cerevisiae plasmid pUR4547, which drives the secretion of V H H domains without any tags.
  • pUR5251, pUR5252, pUR5253, pUR5254, pUR5255, pUR5256 were obtained encoding the V H H domains of clones HGL#1, HGL#8, HGL#9, HGL#10, HGL#11 and HGL#16 respectively.
  • the Pst I/ Bst EII fragments were also cloned into the episomal S. cerevisiae plasmid pUR4585, which is responsible for the secretion of the V H H domain containing a myc- and a hexahistidine tag at its carboxy-terminus.
  • the clones coded pUR5257, pUR5258, pUR5259, pUR5260, pUR5261 and pUR5262 were obtained containing the V H H encoding inserts of clones HGL#1, HGL#8, HGL#9, HGL#10, HGL#11 and HGL#16 respectively.
  • V H H fragments were purified with IMAC (according to the method described in paragraph 3.2). The yield was determined by measuring the optical density at 280 nm using the calculated molar extinction coefficient.
  • the efficiency of HGL recognition was determined for each individual antibody with ELISA using indirectly coated enzyme ( Fig. 4A ) and the degree of inhibition with the enzyme assay ( Fig. 4B ).
  • the measurement of the inhibition properties of the antibodies in accordance to the invention can be carried out in accordance to the method described in Carriere et al in Gastroenterology 1993: 105: 876-888 .
  • the antibodies anti-HGL8 (example 4) and anti-HPL18 (example 3) were tested for inhibition of fat uptake in an animal model. To ensure maximal lipase inhibition in this initial test, the gastric and pancreatic lipase inhibitors were tested in combination.
  • piglets of approximately 15 Kg were fed with differing amounts of anti-HGL and anti-HPL antibodies as part of a high fat diet. As a control, each animal also received a diet without antibody fragment addition.
  • the antibody fragment dosage was chosen such that there was sufficient antibody present to inhibit all of the gastric or pancreatic lipase, based on the assumption that the piglets produced approximately the same amount of GI tract lipase as humans: 25mg HGL and 250mg HPL per meal.
  • a second dosage of antibody fragment was chosen based on an excess of fragment with respect to lipases.
  • Jugular catheters were inserted under anaesthesia in three approximately 15 kg male piglets.
  • the piglets were allowed 6 days to recover. During this time they were fed 120g twice daily (bigbatterikorrel ID-Lelystad, The Netherlands). On the afternoon preceding the serving of the test food, the feed was limited to 60g.
  • the feed was supplemented by addition of a S . cerevisiae fermentation supernatant derived from S . cerevisiae gal1LEU (a prototrophic strain which does not express lipase inhibiting antibody fragments) to al level of 4%.
  • S . cerevisiae fermentation supernatant derived from S . cerevisiae gal1LEU a prototrophic strain which does not express lipase inhibiting antibody fragments
  • the antibodies were prepared from the supernatants of the appropriate S . cerevisiae transformants by concentration with a dialysis unit (Hemophan fiber dialyzer GFSplus 12, Gambro, Breda) followed by freeze drying. The antibody concentration was adjusted such that the feed could be prepared by addition of 10 ml of antibody containing solution. The dosages of antibodies given are shown in table 1. Table 5.1. The feeding regime for the addition of antibody fragments.

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Claims (11)

  1. Anticorps, ou un de ses fragments, capable de se lier spécifiquement à une ou plusieurs lipases alimentaires humaines, ledit anticorps ou un de ses fragments comprenant un domaine variable de chaîne lourde dérivé d'une immunoglobuline naturellement dépourvue de chaînes légères.
  2. Anticorps, ou un de ses fragments, selon la revendication 1, capable de se lier spécifiquement à une lipase pancréatique humaine.
  3. Anticorps, ou un de ses fragments, selon la revendication 2, comprenant 3 régions CDR, moyennant quoi la CDR3 possède une des séquences suivantes : DVRPYRTSRYLEX3, QVRVRFSSDYTNY, LIRRKFTSEYNEY, LITRWDKSVNDY, RRSNYDRSWGDY, LISSYDGSWNDY, HITPAGSSNYVYGY ou DIRKRFTSGYSHY, où X3 est V ou L ou 1.
  4. Anticorps, ou un de ses fragments, selon la revendication 2, ayant une des séquences
    Figure imgb0094
    Figure imgb0095
    Figure imgb0096
    Figure imgb0097
    Figure imgb0098
    Figure imgb0099
    Figure imgb0100
    Figure imgb0101
    ou
    Figure imgb0102
  5. Anticorps, ou un de ses fragments, selon la revendication 1, capable de se lier spécifiquement à une lipase gastrique humaine.
  6. Anticorps, ou un de ses fragments, selon la revendication 5, comprenant 3 régions CDR, moyennant quoi la CDR3 possède une des séquences suivantes : ARSLX1X2TPTSYDY, RGGLTQYSEHDY, TGAEGHY, TDMGRYGTSEW ; où X1 est V ou E et X2 est Q ou L.
  7. Anticorps, ou un de ses fragments, selon la revendication 6, ayant une des séquences
    Figure imgb0103
    Figure imgb0104
    Figure imgb0105
    Figure imgb0106
    Figure imgb0107
    Figure imgb0108
    Figure imgb0109
    ou
    Figure imgb0110
  8. Produit alimentaire comprenant un anticorps ou un de ses fragments selon l'une ou plusieurs des revendications 1 à 7.
  9. Produit pharmaceutique comprenant un anticorps ou un de ses fragments selon l'une ou plusieurs des revendications 1 à 7.
  10. Utilisation d'un anticorps ou d'un de ses fragments selon les revendications 1 à 7 dans la préparation d'un médicament ou d'un aliment pour inhiber l'activité d'une ou de plusieurs enzymes alimentaires dans le corps humain.
  11. Utilisation d'un produit alimentaire selon la revendication 8 pour le contrôle cosmétique du poids corporel des êtres humains.
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150093336A1 (en) * 2010-03-29 2015-04-02 Vib Vzw Anti-macrophage mannose receptor single variable domains for targeting and in vivo imaging of tumor-associated macrophages

Families Citing this family (121)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE60126148T2 (de) 2000-12-13 2007-05-31 Unilever N.V. Verfahren zur herstellung von heterologen proteinen in fungi
US20040018197A1 (en) * 2002-04-26 2004-01-29 Promega Corporation Treatment for weight loss
US9320792B2 (en) 2002-11-08 2016-04-26 Ablynx N.V. Pulmonary administration of immunoglobulin single variable domains and constructs thereof
AU2003286004A1 (en) 2002-11-08 2004-06-07 Ablynx N.V. Single domain antibodies directed against interferon- gamma and uses therefor
US20060034845A1 (en) 2002-11-08 2006-02-16 Karen Silence Single domain antibodies directed against tumor necrosis factor alpha and uses therefor
EP3299393A1 (fr) 2002-11-08 2018-03-28 Ablynx N.V. Anticorps à domaine unique dirigés contre le facteur alpha de la nécrose tumorale et leurs utilisations
RU2401842C2 (ru) 2004-10-08 2010-10-20 Домантис Лимитед Антагонисты и способы их применения
MX2007006086A (es) * 2004-11-25 2007-07-11 Unilever Nv Anticuerpos de cadena pesada y de dominio.
DK1888640T3 (da) 2005-05-18 2012-06-18 Ablynx Nv Forbedrede nanobodies mod tumornekrosefaktor-alfa
RU2433139C2 (ru) 2005-05-20 2011-11-10 Аблинкс Н.В. Nanobodies tm для лечения заболеваний, опосредованных агрегацией
ES2557056T3 (es) 2006-10-10 2016-01-21 Regenesance B.V. Inhibición del complemento para una mejor regeneración de los nervios
GB0621513D0 (en) 2006-10-30 2006-12-06 Domantis Ltd Novel polypeptides and uses thereof
WO2008074839A2 (fr) 2006-12-19 2008-06-26 Ablynx N.V. Séquences d'acides aminés dirigées contre les gpcr et polypeptides les contenant pour le traitement de maladies et de troubles liés au gpcr
EP2514767A1 (fr) 2006-12-19 2012-10-24 Ablynx N.V. Séquences d'acides aminés dirigées contre une métalloprotéinase de la famille ADAM et polypeptides les comprenant pour le traitement de maladies et troubles liés à ADAM
DK2308514T3 (da) 2007-03-23 2013-09-02 To Bbb Holding B V Konjugater til målrettet lægemiddeltransport gennem blod-hjerne barrieren
CA2691940C (fr) 2007-07-03 2018-03-06 Joost Alexander Kolkman Methodes de fourniture de sequences d'immunoglobuline ameliorees
KR20100097716A (ko) 2007-11-27 2010-09-03 아블린쓰 엔.브이. 이종이량체 사이토킨 및/또는 이의 수용체에 대한 아미노산 서열과 이를 포함하는 폴리펩티드
WO2009109572A2 (fr) * 2008-03-03 2009-09-11 Ablynx Nv Exposition sur phage monovalent de domaines variables simples
WO2009109635A2 (fr) 2008-03-05 2009-09-11 Ablynx Nv Nouveaux complexes dimères de fixation à des antigènes, procédés de fabrication et utilisations associés
JP2011516520A (ja) 2008-04-07 2011-05-26 アブリンクス エン．ヴェー． Ｎｏｔｃｈ経路に指向性を有するアミノ酸配列及びその使用
CA2721202A1 (fr) 2008-04-17 2009-10-22 Hilde Adi Pierrette Revets Peptides capables de se lier a des proteines seriques et composes, constructions et polypeptides les comprenant
EP2403873A1 (fr) 2009-03-05 2012-01-11 Ablynx N.V. Nouveaux complexes dimères de liaison antigénique, méthodes d'obtention/non obtention et leurs utilisations
US9464138B2 (en) 2009-04-30 2016-10-11 Ablynx N.V. Method for the production of domain antibodies
US9150640B2 (en) 2009-07-10 2015-10-06 Ablynx N.V. Method for the production of variable domains
US20120244158A1 (en) 2009-09-03 2012-09-27 Ablynx N.V. Stable formulations of polypeptides and uses thereof
WO2011083141A2 (fr) 2010-01-08 2011-07-14 Ablynx Nv Procédé de production de séquences d'immunoglobulines par utilisation de particules de lipoprotéines
CA2788993A1 (fr) 2010-02-05 2011-08-11 Ablynx N.V. Peptides capables de se lier a la serumalbumine, et composes, constructions, et polypeptides comprenant de tels peptides
EP2533761B1 (fr) 2010-02-11 2019-03-27 Ablynx N.V. Procédés et compositions pour la préparation d'aérosols
US9101674B2 (en) 2010-03-29 2015-08-11 Vib Vzw Targeting and in vivo imaging of tumor-associated macrophages
WO2011161263A1 (fr) 2010-06-25 2011-12-29 Ablynx Nv Compositions pharmaceutiques destinées à une administration par voie cutanée
AU2011293127B2 (en) * 2010-08-27 2016-05-12 Abbvie Stemcentrx Llc Notum protein modulators and methods of use
PL2632946T3 (pl) 2010-10-29 2018-06-29 Ablynx N.V. Sposób wytwarzania pojedynczych domen zmiennych immunoglobulin
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EP3590950A1 (fr) 2011-05-09 2020-01-08 Ablynx NV Procédé de production de domaines variables uniques d'immunoglobulines
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WO2012175740A1 (fr) 2011-06-23 2012-12-27 Ablynx Nv Domaines variables uniques d'immunoglobuline dirigés contre ige
US10138302B2 (en) 2011-09-23 2018-11-27 Ablynx N.V. Methods for treating rheumatoid arthritis by administering interleukin-6 receptor antibodies
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WO2014087010A1 (fr) 2012-12-07 2014-06-12 Ablynx N.V. Polypeptides améliorés dirigés contre ige
AU2014214054B2 (en) 2013-02-05 2018-10-04 The Board Of Trustees Of The Leland Stanford Junior University Muscarinic acetylcholine receptor binding agents and uses thereof
NO2951201T3 (fr) 2013-02-08 2018-03-17
JP6499090B2 (ja) 2013-03-15 2019-04-10 ブイアイビー ブイゼットダブリュＶｉｂ Ｖｚｗ 心血管疾患において使用するための抗マクロファージマンノース受容体単一可変ドメイン
TR201819577T4 (tr) 2013-04-29 2019-01-21 Agrosavfe N V Sfingolipitleri bağlayan antikorlar içeren agrokimyasal bileşimler.
NL1040254C2 (en) 2013-05-17 2014-11-24 Ablynx Nv Stable formulations of immunoglobulin single variable domains and uses thereof.
EP2883883A1 (fr) 2013-12-16 2015-06-17 Cardio3 Biosciences S.A. Cibles thérapeutiques et agents utiles dans le traitement des lésions ischémiques de reperfusion
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WO2016016329A1 (fr) 2014-07-29 2016-02-04 Vrije Universiteit Brussel Fragments d'anticorps radio-marqués destinés à être utilisés pour le pronostic et le diagnostic du cancer, ainsi que dans la prédiction de la réponse à un traitement anticancéreux
BR112017001782A2 (pt) 2014-07-29 2017-11-21 Univ Brussel Vrije fragmentos de anticorpo radiomarcados para uso na prevenção e/ou tratamento de câncer
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PL3233910T3 (pl) 2014-12-19 2020-06-01 Ablynx N.V. Dimery nanociał połączone cysteiną
US11298433B2 (en) 2015-07-17 2022-04-12 Vrije Universiteit Brussel Radiolabelled antibody fragments for use in treating cancer
WO2017089618A1 (fr) 2015-11-27 2017-06-01 Ablynx Nv Polypeptides inhibant le ligand cd40l
WO2017182603A1 (fr) 2016-04-22 2017-10-26 Université Libre de Bruxelles Nouveau biomarqueur exprimé dans les cellules bêta pancréatiques utilisé pour l'imagerie ou le ciblage des cellules bêta
WO2017182605A1 (fr) 2016-04-22 2017-10-26 Université Libre de Bruxelles Nouveau biomarqueur exprimé dans les cellules bêta pancréatiques utilisé pour l'imagerie ou le ciblage des cellules bêta
US20190127447A1 (en) 2016-05-02 2019-05-02 Ablynx N.V. Treatment of rsv infection
WO2018007442A1 (fr) 2016-07-06 2018-01-11 Ablynx N.V. Traitement de maladies associées à l'il-6r
WO2018029182A1 (fr) 2016-08-08 2018-02-15 Ablynx N.V. Anticorps à domaine variable unique d'il-6r pour le traitement de maladies liées à l'il-6r
US11098113B2 (en) 2016-09-15 2021-08-24 Vib Vzw Immunoglobulin single variable domains directed against macrophage migration inhibitory factor
WO2018091606A1 (fr) 2016-11-16 2018-05-24 Ablynx Nv Polypeptides de recrutement de lymphocytes t capables de se lier à cd123 et tcr alpha/bêta
WO2018099968A1 (fr) 2016-11-29 2018-06-07 Ablynx N.V. Traitement d'une infection par le virus respiratoire syncytial (vrs)
CA3054623A1 (fr) 2017-02-28 2018-09-07 Vib Vzw Moyens et procedes d'administration de proteines par voie orale
CA3058290A1 (fr) 2017-04-18 2018-10-25 Universite Libre De Bruxelles Biomarqueurs et cibles de maladies proliferatives
US11891451B2 (en) 2017-05-11 2024-02-06 Vib Vzw Glycosylation of variable immunoglobulin domains
EP3630178A1 (fr) 2017-06-02 2020-04-08 Merck Patent GmbH Immunoglobulines de liaison à mmp13
TWI826376B (zh) 2017-06-02 2023-12-21 德商麥克專利有限公司 與ａｄａｍｔｓ５、ｍｍｐ１３及聚集蛋白聚醣結合的多肽
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CN111108126B (zh) 2017-07-19 2024-04-26 非营利性组织佛兰芒综合大学生物技术研究所 血清白蛋白结合剂
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EP3758742A1 (fr) 2018-03-01 2021-01-06 Vrije Universiteit Brussel Immunoglobulines se liant au pd-l1 humain
CN116942851A (zh) 2018-03-23 2023-10-27 布鲁塞尔自由大学 Wnt信号传递激动剂分子
EP3773665A1 (fr) 2018-03-27 2021-02-17 UMC Utrecht Holding B.V. Thrombolyse ciblée pour le traitement d'une thrombose microvasculaire
US20220244254A1 (en) 2019-04-29 2022-08-04 Confo Therapeutics N.V. Screening Methods and Assays for Use with Transmembrane Proteins, in Particular with GPCRs
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US20220220197A1 (en) 2019-05-28 2022-07-14 Vib Vzw Cancer Treatment by Targeting Plexins in the Immune Compartment
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WO2021123360A1 (fr) 2019-12-20 2021-06-24 Vib Vzw Chromatographie par échange de nanocorps
WO2021140205A1 (fr) 2020-01-10 2021-07-15 Confo Therapeutics N.V. Procédés de génération d'anticorps et de fragments d'anticorps et bibliothèques les comprenant
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US20230279115A1 (en) 2020-04-22 2023-09-07 Mabwell (shanghai) Bioscience Co., Ltd. Single variable domain antibody targeting human programmed death ligand 1 (pd-l1) and derivative thereof
WO2021229104A1 (fr) 2020-05-15 2021-11-18 Université de Liège Anticorps anti-cd38 à domaine unique pour la surveillance et le traitement de maladies
WO2022003156A1 (fr) 2020-07-02 2022-01-06 Oncurious Nv Liants non bloquants ccr8
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Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994001567A1 (fr) 1992-07-08 1994-01-20 Unilever N.V. Procede pour immobiliser des enzymes sur la paroi cellulaire d'une cellule microbienne en produisant une proteine de fusion
EP0584421A1 (fr) 1992-08-21 1994-03-02 Cécile Casterman Immunoglobulines dépourvus de chaînes légères
ATE452207T1 (de) 1992-08-21 2010-01-15 Univ Bruxelles Immunoglobuline ohne leichte ketten
WO1994025591A1 (fr) * 1993-04-29 1994-11-10 Unilever N.V. PRODUCTION D'ANTICORPS OU DE FRAGMENTS FONCTIONNALISES D'ANTICORPS, DERIVES DES IMMUNOGLOBULINES A CHAINE LOURDE DE $i(CAMELIDAE)
WO1997049805A2 (fr) 1996-06-27 1997-12-31 Vlaams Interuniversitair Instituut Voor Biotechnologie Vzw Molecules de reconnaissance ayant une interaction specifique avec le site actif ou la fissure d'une molecule cible
ES2239799T3 (es) 1997-02-05 2005-10-01 F. Hoffmann-La Roche Ag Uso de tetrahidrolipstatina en el tratamiento de la diabetes tipo ii.
WO1998056928A1 (fr) * 1997-06-13 1998-12-17 Stichting Instituut Voor Dierhouderij En Diergezondheid Production accrue de proteines au moyen de proteines du type chaperone
EP0954978B1 (fr) 1998-03-12 2011-11-30 VHsquared Limited Produits contenant des levures ou des moisissures inactivées, ayant sur leur surface externe des anticorps actifs
US6558936B1 (en) * 1999-10-01 2003-05-06 Millennium Pharmaceuticals, Inc. Human lipase proteins, nucleic acids encoding them, and uses of both of these

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150093336A1 (en) * 2010-03-29 2015-04-02 Vib Vzw Anti-macrophage mannose receptor single variable domains for targeting and in vivo imaging of tumor-associated macrophages
US9556273B2 (en) * 2010-03-29 2017-01-31 Vib Vzw Anti-macrophage mannose receptor single variable domains for targeting and in vivo imaging of tumor-associated macrophages

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US20040002583A1 (en) 2004-01-01
US7361741B2 (en) 2008-04-22
EP1134231A1 (fr) 2001-09-19
ATE428733T1 (de) 2009-05-15
ES2324280T3 (es) 2009-08-04
DE60138333D1 (de) 2009-05-28

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