EP1092041A4 - EXONUCLEASE-MEDIATED ASSEMBLY OF NUCLEIC ACIDS IN THE DIRECT EVOLUTION - Google Patents

EXONUCLEASE-MEDIATED ASSEMBLY OF NUCLEIC ACIDS IN THE DIRECT EVOLUTION

Info

Publication number
EP1092041A4
EP1092041A4 EP00919763A EP00919763A EP1092041A4 EP 1092041 A4 EP1092041 A4 EP 1092041A4 EP 00919763 A EP00919763 A EP 00919763A EP 00919763 A EP00919763 A EP 00919763A EP 1092041 A4 EP1092041 A4 EP 1092041A4
Authority
EP
European Patent Office
Prior art keywords
exonuclease
nucleic acid
directed evolution
mediated nucleic
acid reassembly
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP00919763A
Other languages
German (de)
English (en)
French (fr)
Other versions
EP1092041A1 (en
Inventor
Jay M Short
Tsotne David Djavakhishvili
Gerhard Johann Frey
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BASF Enzymes LLC
Original Assignee
Diversa Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/276,860 external-priority patent/US6352842B1/en
Priority claimed from US09/332,835 external-priority patent/US6537776B1/en
Application filed by Diversa Corp filed Critical Diversa Corp
Publication of EP1092041A1 publication Critical patent/EP1092041A1/en
Publication of EP1092041A4 publication Critical patent/EP1092041A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Ecology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP00919763A 1999-03-26 2000-03-27 EXONUCLEASE-MEDIATED ASSEMBLY OF NUCLEIC ACIDS IN THE DIRECT EVOLUTION Withdrawn EP1092041A4 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US276860 1999-03-26
US09/276,860 US6352842B1 (en) 1995-12-07 1999-03-26 Exonucease-mediated gene assembly in directed evolution
US09/332,835 US6537776B1 (en) 1999-06-14 1999-06-14 Synthetic ligation reassembly in directed evolution
US332835 1999-06-14
PCT/US2000/008245 WO2000058517A1 (en) 1999-03-26 2000-03-27 Exonuclease-mediated nucleic acid reassembly in directed evolution

Publications (2)

Publication Number Publication Date
EP1092041A1 EP1092041A1 (en) 2001-04-18
EP1092041A4 true EP1092041A4 (en) 2001-09-19

Family

ID=26958167

Family Applications (1)

Application Number Title Priority Date Filing Date
EP00919763A Withdrawn EP1092041A4 (en) 1999-03-26 2000-03-27 EXONUCLEASE-MEDIATED ASSEMBLY OF NUCLEIC ACIDS IN THE DIRECT EVOLUTION

Country Status (6)

Country Link
EP (1) EP1092041A4 (es)
JP (1) JP2004500019A (es)
AU (1) AU4039400A (es)
CA (1) CA2329122A1 (es)
MX (1) MXPA00011569A (es)
WO (1) WO2000058517A1 (es)

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US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US6991922B2 (en) 1998-08-12 2006-01-31 Proteus S.A. Process for in vitro creation of recombinant polynucleotide sequences by oriented ligation
US6951719B1 (en) 1999-08-11 2005-10-04 Proteus S.A. Process for obtaining recombined nucleotide sequences in vitro, libraries of sequences and sequences thus obtained
CA2345203A1 (en) 1998-10-07 2000-04-13 Maxygen Inc. Dna shuffling to produce nucleic acids for mycotoxin detoxification
US6368861B1 (en) 1999-01-19 2002-04-09 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
MXPA00009723A (es) * 1999-02-04 2003-09-22 Diversa Corp Generacion no estocastica de vacunas y enzimas geneticas.
US6531316B1 (en) 1999-03-05 2003-03-11 Maxyag, Inc. Encryption of traits using split gene sequences and engineered genetic elements
DE19953854C2 (de) * 1999-11-09 2002-01-17 Max Planck Gesellschaft Verfahren zur Herstellung von Biopolymeren mit veränderten Eigenschaften
US6686515B1 (en) 1999-11-23 2004-02-03 Maxygen, Inc. Homologous recombination in plants
US6479262B1 (en) 2000-05-16 2002-11-12 Hercules, Incorporated Solid phase enzymatic assembly of polynucleotides
US6858422B2 (en) 2000-07-13 2005-02-22 Codexis, Inc. Lipase genes
EP1507870A4 (en) * 2000-12-01 2005-11-09 Diversa Corp ENZYMS WITH DEHALOGENASE ACTIVITY AND METHOD FOR THEIR USE
WO2002059297A2 (en) 2001-01-25 2002-08-01 Evolva Biotech A/S A library of a collection of cells
US8008459B2 (en) 2001-01-25 2011-08-30 Evolva Sa Concatemers of differentially expressed multiple genes
BR0209154A (pt) 2001-05-18 2004-07-20 Danisco Processo de preparação de uma massa com uma enzima
EP1840211A1 (en) 2001-10-31 2007-10-03 Danisco A/S Pyranosone dehydratase from phanerochaete chrysosporium
ATE506440T1 (de) 2003-01-17 2011-05-15 Danisco Verfahren zur herstellung eines hydroxysäureesters
WO2005066347A1 (en) 2003-12-24 2005-07-21 Danisco A/S Proteins
DK1776455T3 (da) 2004-07-16 2015-06-22 Dupont Nutrition Biosci Aps Lipolytisk enzym, anvendelser deraf i fødevareindustrien
GB0421598D0 (en) 2004-09-29 2004-10-27 Cambridge Advanced Tech Modification of plant development and morphology
GB0422052D0 (en) 2004-10-04 2004-11-03 Dansico As Enzymes
GB0423139D0 (en) 2004-10-18 2004-11-17 Danisco Enzymes
GB0500417D0 (en) 2005-01-10 2005-02-16 Cambridge Antibody Tech Method of mutagenesis
DE602006013596D1 (de) * 2005-01-10 2010-05-27 Medimmune Ltd Cambridge Mutageneseverfahren
BRPI0720801A2 (pt) 2007-01-25 2014-09-16 Dupont Nutrition Biosci Aps Produção de um lipídio aciltranfersase a partir de células transformadas de bacillus licheniformis.
US8143046B2 (en) 2007-02-07 2012-03-27 Danisco Us Inc., Genencor Division Variant Buttiauxella sp. phytases having altered properties
US9476032B2 (en) 2007-03-30 2016-10-25 The Research Foundation For The State University Of New York Attenuated viruses useful for vaccines
EA020035B1 (ru) 2007-12-21 2014-08-29 ДюПон НЬЮТРИШН БАЙОСАЙЕНСИЗ АпС Способы
ES2661310T3 (es) 2009-03-09 2018-03-28 Bioatla, Llc Proteínas mirac
GB0908770D0 (en) 2009-04-24 2009-07-01 Danisco Method
EP2432876A1 (en) 2009-05-19 2012-03-28 Danisco A/S Use
AR077042A1 (es) 2009-06-12 2011-07-27 Danisco Metodo para tratar una composicion que contiene pirofiofitina
GB0920089D0 (en) 2009-11-17 2009-12-30 Danisco Method
WO2011110967A1 (en) 2010-03-12 2011-09-15 Danisco A/S Process
WO2011114251A1 (en) 2010-03-18 2011-09-22 Danisco A/S Foodstuff
AR081950A1 (es) 2010-06-17 2012-10-31 Danisco Proceso de tratamiento de semillas que contienen aceite
GB201011513D0 (en) 2010-07-08 2010-08-25 Danisco Method
CA2825628A1 (en) 2011-02-23 2012-08-30 Dupont Nutrition Biosciences Aps Method
WO2012114232A1 (en) 2011-02-23 2012-08-30 Dupont Nutrition Biosciences Aps Process
ES2649912T3 (es) 2011-02-23 2018-01-16 Dupont Nutrition Biosciences Aps Tratamiento enzimático de la clorofila en los aceites vegetales
RU2013149861A (ru) 2011-04-08 2015-05-20 ДАНИСКО ЮЭс, ИНК. Композиции
WO2013104659A2 (en) 2012-01-13 2013-07-18 Dupont Nutrition Biosciences Aps Process
WO2013104660A1 (en) 2012-01-13 2013-07-18 Dupont Nutrition Biosciences Aps Process for treating a plant oil comprising hydrolysing chlorophyll or a chlorophyll derivative and involving partial caustic neutralisation
WO2013160374A1 (en) 2012-04-27 2013-10-31 Dupont Nutrition Biosciences Aps Process for refining crude plant oil involving enzymatic hydrolysis and gum recycling
WO2013160372A1 (en) 2012-04-27 2013-10-31 Dupont Nutrition Biosciences Aps Process for treating plant oil involving addition of serial doses of chlorophyll or chlorophyll derivative degrading enzyme
EP2909230B1 (en) 2012-10-19 2019-05-22 Danisco US Inc. Stabilization of biomimetic membranes
DK3083936T3 (en) 2013-12-19 2018-10-22 Danisco Us Inc USE OF HYDROPHOBINES TO INCREASE GAS TRANSFER IN AEROBE FERMENTATION PROCESSES
WO2015150372A1 (en) 2014-04-01 2015-10-08 Dupont Nutrition Biosciences Aps Method for increasing crude palm oil yields
BR112017007949A2 (pt) 2014-10-24 2018-01-23 Danisco Us Inc método para produção de álcool pelo uso de uma tripeptidil-peptidase
EP3209773B1 (en) 2014-10-24 2020-03-18 DuPont Nutrition Biosciences ApS Proline tolerant tripeptidyl peptidases and uses thereof
MX2018009216A (es) 2016-02-25 2018-11-09 Dupont Nutrition Biosci Aps Metodo para producir un hidrolizado de proteina empleando una tripeptidil peptidasa de aspergillus fumigatus.
EP3491128B1 (en) * 2016-07-29 2021-09-08 New England Biolabs, Inc. Methods and compositions for preventing concatemerization during template- switching

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0316018A2 (en) * 1985-03-29 1989-05-17 Cetus Oncology Corporation Modification of DNA sequences
EP0438292A2 (en) * 1990-01-17 1991-07-24 NIHON CHEMICAL RESEARCH KABUSHIKI KAISHA also known as JCR PHARMACEUTICALS CO., LTD Method of preparing primers for polymerase chain reaction and DNA amplification using said primers
WO1991012341A1 (en) * 1990-02-12 1991-08-22 Fred Hutchinson Cancer Research Center Improved method for ordered deletions from a fixed point in a cloned insert
WO1997020078A1 (en) * 1995-11-30 1997-06-05 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
WO1997020918A1 (en) * 1995-12-07 1997-06-12 Recombinant Biocatalysis, Inc. Method of screening for enzyme activity
WO1997020950A1 (en) * 1995-12-08 1997-06-12 Stratagene Improved circular site-directed mutagenesis
WO1998001581A1 (en) * 1996-07-09 1998-01-15 Recombinant Biocatalysis, Inc. Method of dna shuffling with polynucleotides produced by blockingor interrupting a synthesis or amplification process
WO1998038297A1 (en) * 1997-02-27 1998-09-03 Gesher - Israel Advanced Biotecs (1996) Ltd. Single step assembly of multiple dna fragments
WO1998058080A1 (en) * 1997-06-16 1998-12-23 Bioinvent International Ab A method for in vitro molecular evolution of protein function

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4959312A (en) * 1985-05-31 1990-09-25 The University Of Tennessee Research Corporation Full spectrum mutagenesis
US5830696A (en) * 1996-12-05 1998-11-03 Diversa Corporation Directed evolution of thermophilic enzymes

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0316018A2 (en) * 1985-03-29 1989-05-17 Cetus Oncology Corporation Modification of DNA sequences
EP0438292A2 (en) * 1990-01-17 1991-07-24 NIHON CHEMICAL RESEARCH KABUSHIKI KAISHA also known as JCR PHARMACEUTICALS CO., LTD Method of preparing primers for polymerase chain reaction and DNA amplification using said primers
WO1991012341A1 (en) * 1990-02-12 1991-08-22 Fred Hutchinson Cancer Research Center Improved method for ordered deletions from a fixed point in a cloned insert
WO1997020078A1 (en) * 1995-11-30 1997-06-05 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
WO1997020918A1 (en) * 1995-12-07 1997-06-12 Recombinant Biocatalysis, Inc. Method of screening for enzyme activity
WO1997020950A1 (en) * 1995-12-08 1997-06-12 Stratagene Improved circular site-directed mutagenesis
WO1998001581A1 (en) * 1996-07-09 1998-01-15 Recombinant Biocatalysis, Inc. Method of dna shuffling with polynucleotides produced by blockingor interrupting a synthesis or amplification process
WO1998038297A1 (en) * 1997-02-27 1998-09-03 Gesher - Israel Advanced Biotecs (1996) Ltd. Single step assembly of multiple dna fragments
WO1998058080A1 (en) * 1997-06-16 1998-12-23 Bioinvent International Ab A method for in vitro molecular evolution of protein function

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
G.P. SMITH: "The progeny of sexual PCR", NATURE, vol. 370, 4 August 1994 (1994-08-04), pages 324 - 325 *
PALERMO D.P.; HESS G.F.: "Use of lambda exonuclease for efficient oligonucleotide-mediated site-directed deletion and point mutation of double stranded DNA", DNA, vol. 6, no. 3, pages 273 - 279 *
See also references of WO0058517A1 *
STEMMER W: "DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA., vol. 91, no. 22, 25 October 1994 (1994-10-25), pages 10747 - 10751, XP002170160 *
WAYNE M. COCO ET AL.: "DNA shuffling method for generating highly recombined genes and evolved enzymes", NATURE BIOTECHNOLOGY, vol. 19, no. 4, pages 354 - 359 *

Also Published As

Publication number Publication date
CA2329122A1 (en) 2000-10-05
MXPA00011569A (es) 2004-12-03
AU4039400A (en) 2000-10-16
JP2004500019A (ja) 2004-01-08
EP1092041A1 (en) 2001-04-18
WO2000058517A1 (en) 2000-10-05

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