Classifications

• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
  • A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
  • A61K47/08—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing oxygen, e.g. ethers, acetals, ketones, quinones, aldehydes, peroxides
  • A61K47/12—Carboxylic acids; Salts or anhydrides thereof
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/33—Heterocyclic compounds
  • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
  • A61K31/53—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with three nitrogens as the only ring hetero atoms, e.g. chlorazanil, melamine
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K9/00—Medicinal preparations characterised by special physical form
  • A61K9/0012—Galenical forms characterised by the site of application
  • A61K9/0019—Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P35/00—Antineoplastic agents

Definitions

• the present invention relates to the field of treatments for cancer tumors. More particularly, the present invention relates to treatment of cancer tumors with 1,2,4- benzotriazine oxides contained in an aqueous buffered vehicle.
• R and R is hydrogen, halogen, lower alkyl, halo (lower alkyl), lower alkoxy, carbamoyl, sulfonamido, carboxy or carbo (lower alkoxy) and the other of R and R is halogeno, lower alkyl, halo (lower alkyl), lower alkoxy, carbamoyl, sulfonamido, carboxy or carbo (lower alkoxy), as antimicrobial composition used to promote livestock growth.
• U.S. Patent, 5,175,287 issued December 29, 1992 discloses the use of 1,2,4- benzotriazine oxides in conjunction with radiation for treatment of tumors.
• the 1,2,4- benzotriazine oxides sensitize the tumor cells to radiation and make them more amenable to this treatment modality.
• Radiation therapy and chemotherapy along with surgery, remain the three primary modalities in the treatment of cancer Radiation therapy and chemotherapy function as alternatives to surgery in the primary control of a variety of neoplasms, where surgery is limited by anatomic consideration
• Current knowledge demonstrates that higher cure rates and greater quality of life could be afforded to cancer patients if the effectiveness of radiation therapy and chemotherapy were improved
• hypoxia is a malignant neoplasm originating from tumor cells.
• a method that augments the kill of hypoxic tumor cells (or limits the radiation damage to normal tissues) would improve the therapeutic index of radiation or chemotherapy.
• the benzotriazine compounds have been developed to take advantage of this relative hypoxia within the tumor Tirapazamine, the most promising member of the benzotriazine series to date, is bioreduced under conditions of hypoxia to an active intermediate. This active intermediate can induce DNA damage, which enhances the effects of radiation therapy or chemotherapy and is cytotoxic in its own right. Because adjacent normal tissues are not hypoxic, this bioreduction allows for selective cytotoxic effects on hypoxic tumor cells.
• Tirapazamine (Benzotriazine di-N-oxide) 75-200 15-100
• RSU-1069 (Nitroimidazole/Aziridine) 75-100 10-20
• Nitracrine (Nitroacridine) 7 Nitracrine (Nitroacridine) 7 —
• Tirapazamine however, has the drawbacks of insufficient solubility in pharmaceutical vehicles suitable for parenteral administration as well as being unstable in such vehicles. It has been found that the solubility of tirapazamine in water is about 0.81 mg/ml, which would required a large volume of the solution, approximately, 1 liter, to be administered to a patient for providing the proper dose. Attempts to enhance the solubility using surfactants such as Tween 80, and polymers such as Pluronic F68, Povidone and Albumin were unsuccessful with minimal increase in solubility.
• the present invention has as its main object to provide an aqueous infusable/injectable formulation which contains sufficient amounts of the anticancer tumor agent and is stable on shelf-life. During our extensive clinical studies of tirapazamine it was realized that without sufficient solubility and stability this very promising drug would not help the countless patients suffering from cancer tumor.
• the present invention provides an aqueous parenteral formulation for the treatment of cancer tumors comprising: an effective cancer tumor treating amount of a compound of the formula (I)
• X is H; halogen; alkoxy (C1-C4); hydrocarbyl (C1-C4); OR; COR 1 ; or NR 2 R 3 ; n is 0 or 1 ; and Y 1 and Y 2 are independently H; nitro; halogen, alkoxy (C1-C4), hydrocarbyl (Cl-
• C14 optionally interrupted by a single ether linkage; OR ; COR ; NR R ; morpholino; pyrrolidino; piperidino; acyloxy (C1-C4); acylamido (C1-C4) and thio analogs thereof; acetylaminoalkyl (C1-C4); carboxy; alkoxycarbonyl (C1-C4); carbamyl; alkylcarbonyl (Cl-
• R-R 7 can be independently selected from: H, alkyl (C1-C4), acyl (C1-C4), or R 2 and
• R or R and R taken together directly or through a bridge oxygen atom form a morpholino, pyrrolidino or piperidino ring, and where R and R also can represent hydrocarbyl (C1-C4) unsubstituted or substituted with substituents such as described below, morpholino, pyrrolidino or piperidino, and R 8 -R 14 independently represent hydrocarbyl (C1-C4).
• X, Y 1 and Y 2 can be unsubstituted or substituted with substituents such as OH, halogen (Cl, Br, I, F), NH 2 , alkyl (C1-C4), alkoxy (C1-C4), alkyl secondary amino, dialkyltertiary amino, or a pharmacologically acceptable salt of said compound in a parenterally acceptable buffer having a concentration of from about 0.001M to about 0.1M.
• substituents such as OH, halogen (Cl, Br, I, F), NH 2 , alkyl (C1-C4), alkoxy (C1-C4), alkyl secondary amino, dialkyltertiary amino, or a pharmacologically acceptable salt of said compound in a parenterally acceptable buffer having a concentration of from about 0.001M to about 0.1M.
• Other formulations within the ambit of the present invention are those comprising: an effective cancer tumor treating amount of a compound of the formula (
• X is hydrocarbyl (C1-C4) substituted by halogen, alkyl (C1-C4) or alkoxy (C1-C4); O-acyl(Cl-C4); or COR 1 ; n is 0 or 1 ; and
• Y 1 and Y 2 are independently H; nitro; halogen, alkoxy (C1-C4), hydrocarbyl (Cl- C14), optionally interrupted by a single ether linkage; OR ; COR ; NR R ; morpholino; pyrrolidino; piperidino; acyloxy (C1-C4); acylamido (C1-C4) and thio analogs thereof; acetylaminoalkyl (C1-C4); carboxy; alkoxycarbonyl (C1-C4); carbamyl; alkylcarbonyl (Cl- C4); alkylsulfonyl (C1-C4); alkylphosphonyl (C1-C4); NR 8 R 9 O(CO)R 10 ; NH(CO)R H ; O(SO)R 12 ; O(POR 13 )R 14 ; wherein R i -R 7 can be independently selected from: H, alkyl (C
• R or R and R taken together directly or through a bridge oxygen atom form a morpholino, 1 pyrrolidino or piperidino ring, and where R and R also can represent hydrocarbyl (C1-C4) unsubstituted or substituted with substituents selected from those described below, morpholino, pyrrolidino or piperidino, and R 8 -R 14 independently represent hydrocarbyl (Cl- C4); and
• Y 1 and Y 2 can be unsubstituted or substituted with substituents selected from OH, halogen (Cl, Br, I, F), NH 2 , alkyl (C1-C4), alkoxy (C1-C4), alkylsecondary amino, dialkyltertiary amino, or a pharmacologically acceptable salt of said compound in a parenterally acceptable buffer having a concentration of from about 0.001M to about 0.1M.
• substituents selected from OH, halogen (Cl, Br, I, F), NH 2 , alkyl (C1-C4), alkoxy (C1-C4), alkylsecondary amino, dialkyltertiary amino, or a pharmacologically acceptable salt of said compound in a parenterally acceptable buffer having a concentration of from about 0.001M to about 0.1M.
• the parenteral formulation for the treatment of cancer tumors of the present invention comprises: of from about 0.500 to about 0.810 g of a compound of the formula (I); of from about 0.100 to about 9.000 g of sodium chloride; of from about 0.1 to about 10.00 g of citric acid; of from about 0.02 to about 3.00 g of sodium hydroxide; and qs to pH 3.0 - 5.0 in water to 1000 ml.
• the preferred anticancer tumor compound of the present invention is tirapazamine, l,2,4-benzotriazine-3-amine 1,4-dioxide, having the structural formula with molecular weight of 178.16 and melting point on decomposition of 220°C.
• each milliliter of solution contains from about 0.7 to about 0.81 mg/ml tirapazamine in an isotonic citrate buffer having a pH of from about 3.7 to about 4.3.
• the present invention is also directed to a method of cancer tumor treatment of a patient in need of such treatment comprising administering an effective cancer tumor treating amount of a formulation to said patient.
• the present invention provides a composition and a method for treating mammalian cancer tumors, including human cancer tumors, particularly solid tumors.
• an effective amount of a compound having Formula I, as defined herein, contained in a citrate buffer solution is administered to a mammal having a cancer tumor and in need of such treatment from about one half hour to about twenty-four hours before an effective amount of a chemotherapy agent to which the tumor is susceptible is administered to the mammal.
• Formula I and testing of a compound is described in U.S. Application Serial No. 125,609 filed on September 22, 1993, the disclosure of which in its entirety is incorporated herein by reference.
• extensive studies were conducted to provide sufficient solubility of the cancer tumor compound and render the formulation stable on shelf-life as will become clear from the description that follows.
• Another preferred anticancer tumor compound of the present invention is 3-(2-methoxyethyl)-l,2,4-benzotriazine 1,4-dioxide having the structural formula
• Ethanol 15 0.47 The limited solubility of 0.81 mg/ml would require up to a liter of fluid to be infused, therefore in order to minimize the fluid volume, solubility needed to be increased. Attempts to enhance the solubility by using surfactants (Tween 80) and polymers (Pluronic F68, Povidone, Albumin) were unsuccessful with minimal increase in solubility.
• the physicochemical properties of tirapazamine demonstrate that the molecule is neither highly polar nor highly lipophilic in character. This is illustrated by (i) the partition coefficient (octanol/water) of 0.15 (logP -0.82) and (ii) the observed decomposition on melting at 200°C which suggests the crystal structure of tirapazamine is strongly bound by intermolecular forces.
• the planar nature of the molecule would facilitate an ordered stacking with the crystal with intermolecular attractions (charge transfer interactions) between each plane via the nitrogen and oxygen of the N-oxide functions.
• a hydrated form of tirapazamine can exist where water molecules are hydrogen bonded to the oxygen components.
• PEG400 polyethylene glycol 400
• aprotic solvents e.g., dimethylsulphoxide (DMSO), dimethylformamide (DMF) and dimethylacetamide (DMA)
• DMSO dimethylsulphoxide
• DMF dimethylformamide
• DMA dimethylacetamide
• Amphiprotic solvents e.g., glycerol, PEG 400 and propylene glycol (PG) can both self-associate and hydrogen bond with water, consequently, such solvents are not ideally suited for solutes that cannot participate in hydrogen bonding.
• the partition coefficient of the solute is an indicator for predicting whether co-solvents will be effective.
• Formulations were also stressed by storing at elevated temperatures of 50°C and 70°C after a single autoclave cycle of 21 minutes at 121 °C. Tirapazamine was found to be unstable in the presence of lactate buffer after storage at 70°C. This instability was not apparent from multiple autoclave stressing. The most stable formulation was found to be 0.05M citrate pH 4. Formulation of tirapazamine was therefore progressed using citrate buffer. Solubility of tirapazamine at 15°C required the concentration to be reduced from 1 to 0.5 mg/ml. Further stressing in citrate buffer at pH 3.5, 4.0 and 4.5 was conducted to determine the likely limits for pH. Based on data from this study the limits were set at pH 4.0 ⁇ 0.3.
• Tirapazamine was stable after 2 months in both 0.005M and 0.05M citrate buffer at 50°C. At 70°C, there was evidence of instability with the 0.05M citrate formulation, therefore the lower citrate concentration (0.005M) was chosen for development as the clinical formulation.
• the clinical formulation used in chemical studies discussed later was as follows: Tirapazamine 0.700g
• Tirapazamine 0.1500g qs to pH 4.0 in water to 1000 ml.
• Tirapazamine is stored in clear glass 20 ml ampoules containing 0.7 mg/ml (14 mg) of tirapazamine in the isotonic citrate buffer. The ampoules are stored at 15°C to 30°C in light- proof packaging.
• the LDio and LD50 for tirapazamine were found to be 98 and 101 mg/kg, respectively.
• Single and 2-week and 2-month multiple-dose studies were performed in the rat and the dog.
• Clinical signs and symptoms observed in both species and each regimen included salivation, decreases in white blood cell measurements (including lymphocyte count in the dog), and decreases in red blood cell measurements.
• Tirapazamine (20 ⁇ M) was a potent and selective killer of hypoxic cells in vitro, with hypoxic cytotoxicity ratios of 150, 119 and 52 for hamster, mouse and human cell lines, respectively (1-2 orders of magnitude greater than radiation sensitizers such as nitroimidazoles, mitomycin C and porfiromycin). This cytotoxicity was also observed over a range of oxygen tensions (l%-20% O 2 ; primarily at l%-4% O 2 ).
• tirapazamine was equally effective in mouse tumor models as a single 0.30 mmol/kg (160mg/m 2 ) dose or as multiple 0.08 mmol/kg (43 mg/m 2 ) doses, when used with fractionated radiation (2.5 Gy x 8). Tirapazamine was also effective as a single 0.30 mmol/kg (160mg/m 2 ) dose with a single large (20 Gy) does of radiation.
• Tirapazamine appeared to be most effective, resulting in several cures in mouse SCCVII tumors, as multiple 0.08 mmol/kg (43 mg/m 2 ) doses given prior to each radiation fraction (2.5 Gy x 8); and tirapazamine appeared least effective, resulting in typically less than 1 log of cell kill, when given without radiation.
• tirapazamine produced an effect equal to the effect predicted if tirapazamine were acting upon a separate cell population (hypoxic cells) than the radiation was acting upon (aerobic cells).
• tirapazamine The mechanism of action of tirapazamine has been studied in detail and is closely tied to the metabolism of the drug.
• the illustration below portrays the proposed mechanism of action for tirapazamine-production of a free radical, during reduction to the mono-N-oxide, which causes single- and double-strand breaks in DNA.
• tirapazamine is metabolized to the 2-electron reduction product WIN 64102 (mono-N-oxide; SR 4317) and then to the 4-electron reduction product WIN 60109 (zero-N-oxide; SR 4330).
• the benzotriazine di-N-oxide tirapazamine was extensively studied both in vitro and in vivo to determine and quantify its effectiveness and to elucidate its mechanism of action.
• a Hypoxic cytotoxicity ratio Concentration of tirapazamine in air/Concentration of tirapazamine in nitrogen to yield approximately the same survival.
• Sensitivity index Time (in minutes) to reach 10" 2 (1%) surviving fraction at 20 ⁇ M under hypoxic conditions,
• c IC50 Concentration required to inhibit cell growth by 50% in a 1-hour incubation under hypoxic conditions.
• d Normal nontumorigenic.
• tirapazamine augments the antitumor activity of radiation, assessed by cell killing or tumor growth delay.
• Tumors tested include
• Tirapazamine augments cell kill when given on a single- or multiple-dose schedule, and when the drug is combined with either single-dose or fractionated radiation.
• tirapazamine plus radiation exceeds the additive effect of these two treatments. Augmentation of activity by tirapazamine occurs when the drug is administered form 2.5 to 0.5 hours before radiation or up to 6 hours afterward.
• tirapazamine radiosensitizes aerobic cells in vitro if the cells are exposed to the drug under hypoxic conditions either before or after radiation.
• treatment with tirapazamine enhanced the antitumor activity of radiation to a greater extent than did the hypoxic cell sensitizer etanidazole.
• the oxygen concentration/cytotoxicity curve of tirapazamine appears particularly well suited to combination with radiotherapy. Below approximately 30 torr (mm of Hg) cells become increasingly resistant to damaging effects of radiation. Nitroaromatic and quinone antibiotic radiosensitizers, however, are most effective only at much lower oxygen levels. Thus, they are not toxic to the moderately hypoxic, radioresistant cells present in tumors. By contrast, the cytotoxicity of tirapazamine remains relatively constant over the entire range of oxygen concentrations conferring radio-resistance.
• the toxicity of tirapazamine decreases at high oxygen concentrations (i.e., those found in normal tissue).
• the toxicity of tirapazamine was at least 50 to >2000 fold higher under hypoxia than under 100%) oxygen vapor. Because it is active against a wide range of radio-resistant tumor cells but is not toxic to normal cells with high oxygen concentrations, tirapazamine is selectively cytotoxic to hypoxic tumor cells.
• mice Female C3H/Km mice were used in two assays to examine the potential that tirapazamine might affect normal tissue sensitivity to ionizing radiation. Both normal skin reaction and leg (thigh) contraction tests were conducted with fractionated radiation. Tirapazamine did not affect the tissues in either assay.
• mice were scored “blinded” - with no knowledge of their treatment group-according to a scale similar to one developed previously [Brown JM, Goffinet DR, Cleaver JE, Kallman RF, "Preferential radiosensitization of mouse sarcoma relative to normal mouse skin by chronic intra-arterial infusion of halogenated pyrimidine analogs", JNCI (1971) 47, 77-89]. No radiosensitization or additive toxicity was produced by the addition of tirapazamine to the radiation treatment as determined by skin reaction.

Landscapes

• Health & Medical Sciences (AREA)
• Chemical & Material Sciences (AREA)
• Medicinal Chemistry (AREA)
• Pharmacology & Pharmacy (AREA)
• Life Sciences & Earth Sciences (AREA)
• Animal Behavior & Ethology (AREA)
• General Health & Medical Sciences (AREA)
• Public Health (AREA)
• Veterinary Medicine (AREA)
• Epidemiology (AREA)
• General Chemical & Material Sciences (AREA)
• Chemical Kinetics & Catalysis (AREA)
• Engineering & Computer Science (AREA)
• Oil, Petroleum & Natural Gas (AREA)
• Dermatology (AREA)
• Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
• Organic Chemistry (AREA)
• Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
• Agricultural Chemicals And Associated Chemicals (AREA)
• Medicinal Preparation (AREA)

Applications Claiming Priority (3)

Publications (3)

Family

ID=25275027

Family Applications (1)

Country Status (13)

Families Citing this family (9)

Citations (1)

Family Cites Families (3)

• 1997
  • 1997-04-21 US US08/837,637 patent/US5827850A/en not_active Expired - Fee Related
• 1998
  • 1998-04-14 EP EP98915529A patent/EP1044005B1/fr not_active Expired - Lifetime
  • 1998-04-14 BR BR9808958-7A patent/BR9808958A/pt not_active Application Discontinuation
  • 1998-04-14 DK DK98915529T patent/DK1044005T3/da active
  • 1998-04-14 AT AT98915529T patent/ATE332139T1/de not_active IP Right Cessation
  • 1998-04-14 AU AU69690/98A patent/AU6969098A/en not_active Abandoned
  • 1998-04-14 ES ES98915529T patent/ES2267183T3/es not_active Expired - Lifetime
  • 1998-04-14 PT PT98915529T patent/PT1044005E/pt unknown
  • 1998-04-14 WO PCT/US1998/007391 patent/WO1998047512A1/fr active IP Right Grant
  • 1998-04-14 JP JP54612298A patent/JP2001523248A/ja not_active Ceased
  • 1998-04-14 CA CA002287257A patent/CA2287257C/fr not_active Expired - Fee Related
  • 1998-04-14 DE DE69835167T patent/DE69835167T8/de not_active Expired - Fee Related
  • 1998-09-21 US US09/157,905 patent/US6153610A/en not_active Expired - Fee Related
• 1999
  • 1999-10-20 NO NO19995109A patent/NO325275B1/no unknown

Patent Citations (1)

Non-Patent Citations (1)

Also Published As

Similar Documents

Legal Events