EP1012257A1 - Methodes et materiels pour produire et utiliser des organismes transgeniques degradant dicamba - Google Patents

Methodes et materiels pour produire et utiliser des organismes transgeniques degradant dicamba

Info

Publication number
EP1012257A1
EP1012257A1 EP98915256A EP98915256A EP1012257A1 EP 1012257 A1 EP1012257 A1 EP 1012257A1 EP 98915256 A EP98915256 A EP 98915256A EP 98915256 A EP98915256 A EP 98915256A EP 1012257 A1 EP1012257 A1 EP 1012257A1
Authority
EP
European Patent Office
Prior art keywords
dicamba
dna
degrading
plant
seq
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
EP98915256A
Other languages
German (de)
English (en)
Other versions
EP1012257A4 (fr
Inventor
Donald P. Weeks
Xiao-Zhuo Wang
Patricia L. Herman
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Nebraska
Original Assignee
University of Nebraska
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Nebraska filed Critical University of Nebraska
Priority to EP10182774A priority Critical patent/EP2325308A3/fr
Publication of EP1012257A1 publication Critical patent/EP1012257A1/fr
Publication of EP1012257A4 publication Critical patent/EP1012257A4/fr
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8259—Phytoremediation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

L'invention concerne des enzymes isolées et au moins partiellement purifiées dégradant dicamba, des molécules d'ADN isolées qui codent pour des enzymes dégradant dicamba, des constructions d'ADN qui codent pour des enzymes dégradant dicamba, des cellules hôtes transgéniques comprenant un ADN qui code pour des enzymes dégradant dicamba, et des plantes transgéniques et éléments de plante qui comprennent une ou plusieurs cellules incluant un ADN qui code pour des enzymes dégradant dicamba. L'expression des enzymes dégradant dicamba donne lieu à la production d'organismes dégradant dicamba, y compris des plantes tolérant dicamba. L'invention concerne en outre une méthode qui permet de lutter contre les mauvaises herbes dans un champ contenant lesdites plantes tolérant dicamba, et une méthode pour décontaminer un matériel contenant dicamba, qui consiste à appliquer sur ledit matériel une quantité efficace d'un micro-organisme transgénique ou d'une enzyme dégradant dicamba. L'invention concerne enfin une méthode de sélection de plantes modifiées et de cellules végétales basée sur la tolérance de dicamba et une méthode de sélection et de criblage de cellules hôtes modifiées, d'organismes et d'éléments d'organismes intacts sur la base de la fluorescence de 3,6-acide dichlorosalicylique résultant de la dégradation de dicamba.
EP98915256A 1997-04-04 1998-04-03 Methodes et materiels pour produire et utiliser des organismes transgeniques degradant dicamba Ceased EP1012257A4 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP10182774A EP2325308A3 (fr) 1997-04-04 1998-04-03 Procédés et matériaux pour la fabrication et l'utilisation d'organismes transgéniques dégradant le dicamba

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US4294197P 1997-04-04 1997-04-04
US4266697P 1997-04-04 1997-04-04
US42666P 1997-04-04
US42941P 1997-04-04
PCT/US1998/006589 WO1998045424A1 (fr) 1997-04-04 1998-04-03 Methodes et materiels pour produire et utiliser des organismes transgeniques degradant dicamba

Publications (2)

Publication Number Publication Date
EP1012257A1 true EP1012257A1 (fr) 2000-06-28
EP1012257A4 EP1012257A4 (fr) 2004-08-18

Family

ID=26719500

Family Applications (2)

Application Number Title Priority Date Filing Date
EP10182774A Withdrawn EP2325308A3 (fr) 1997-04-04 1998-04-03 Procédés et matériaux pour la fabrication et l'utilisation d'organismes transgéniques dégradant le dicamba
EP98915256A Ceased EP1012257A4 (fr) 1997-04-04 1998-04-03 Methodes et materiels pour produire et utiliser des organismes transgeniques degradant dicamba

Family Applications Before (1)

Application Number Title Priority Date Filing Date
EP10182774A Withdrawn EP2325308A3 (fr) 1997-04-04 1998-04-03 Procédés et matériaux pour la fabrication et l'utilisation d'organismes transgéniques dégradant le dicamba

Country Status (9)

Country Link
EP (2) EP2325308A3 (fr)
JP (3) JP2001519663A (fr)
KR (1) KR100558838B1 (fr)
AU (1) AU741974B2 (fr)
CA (1) CA2285676C (fr)
NO (1) NO324769B1 (fr)
NZ (1) NZ338093A (fr)
RU (1) RU2226215C2 (fr)
WO (1) WO1998045424A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109971773A (zh) * 2019-03-29 2019-07-05 南阳师范学院 一种编码可降解3-氯龙胆酸的龙胆酸双加氧酶DsmI的基因dsmI及其应用

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JP2005506043A (ja) * 2001-02-28 2005-03-03 ボード・オブ・リージェンツ・オブ・ザ・ユニバーシティ・オブ・ネブラスカ 遺伝子導入ジカンバ分解性生物体を作製及び使用するための方法及び材料
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US7855326B2 (en) 2006-06-06 2010-12-21 Monsanto Technology Llc Methods for weed control using plants having dicamba-degrading enzymatic activity
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CN104630162B (zh) * 2014-12-22 2017-07-25 北京大北农生物技术有限公司 除草剂耐受性蛋白质、其编码基因及用途
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CN111118037B (zh) * 2019-10-14 2021-07-13 中国农业科学院生物技术研究所 具有除草剂麦草畏降解功能的基因dicx4及其应用
CN111139238B (zh) * 2019-10-14 2021-08-03 中国农业科学院生物技术研究所 除草剂麦草畏的降解基因dicX3及其应用
CN111139248B (zh) * 2019-10-14 2021-07-13 中国农业科学院生物技术研究所 dicX5基因及其对除草剂的降解功能
CN111139249B (zh) * 2019-10-14 2021-07-09 中国农业科学院生物技术研究所 运动金球菌麦草畏降解基因dicM的用途
CN110872591B (zh) * 2019-10-14 2021-07-13 中国农业科学院生物技术研究所 除草剂麦草畏降解基因dicX1及其应用

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109971773A (zh) * 2019-03-29 2019-07-05 南阳师范学院 一种编码可降解3-氯龙胆酸的龙胆酸双加氧酶DsmI的基因dsmI及其应用

Also Published As

Publication number Publication date
AU6948498A (en) 1998-10-30
EP1012257A4 (fr) 2004-08-18
CA2285676A1 (fr) 1998-10-15
JP2010187673A (ja) 2010-09-02
WO1998045424A1 (fr) 1998-10-15
NZ338093A (en) 2001-06-29
NO994795L (no) 1999-12-01
RU2226215C2 (ru) 2004-03-27
KR20010006014A (ko) 2001-01-15
KR100558838B1 (ko) 2006-03-10
MX9909086A (fr) 2000-08-31
EP2325308A2 (fr) 2011-05-25
JP2009039123A (ja) 2009-02-26
CA2285676C (fr) 2011-03-08
NO324769B1 (no) 2007-12-10
AU741974B2 (en) 2001-12-13
JP2001519663A (ja) 2001-10-23
EP2325308A3 (fr) 2011-07-27
NO994795D0 (no) 1999-10-01

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