NO324769B1 - Isolert DNA molekyl, DNA konstruksjon, renset dicambadegraderende oksygenase, transgen vertscelle og plante, fremgangsmate for dekontaminering av et materiale inneholdende dicamba, for selektering av transformerte planteceller og for selektering, eller screening for, transformerte vertsceller, intakte organismer og deler av organismer. - Google Patents
Isolert DNA molekyl, DNA konstruksjon, renset dicambadegraderende oksygenase, transgen vertscelle og plante, fremgangsmate for dekontaminering av et materiale inneholdende dicamba, for selektering av transformerte planteceller og for selektering, eller screening for, transformerte vertsceller, intakte organismer og deler av organismer. Download PDFInfo
- Publication number
- NO324769B1 NO324769B1 NO19994795A NO994795A NO324769B1 NO 324769 B1 NO324769 B1 NO 324769B1 NO 19994795 A NO19994795 A NO 19994795A NO 994795 A NO994795 A NO 994795A NO 324769 B1 NO324769 B1 NO 324769B1
- Authority
- NO
- Norway
- Prior art keywords
- dicamba
- plant
- organisms
- dna
- cells
- Prior art date
Links
- IWEDIXLBFLAXBO-UHFFFAOYSA-N dicamba Chemical compound COC1=C(Cl)C=CC(Cl)=C1C(O)=O IWEDIXLBFLAXBO-UHFFFAOYSA-N 0.000 title claims description 134
- 239000005504 Dicamba Substances 0.000 title claims description 130
- 108090000417 Oxygenases Proteins 0.000 title claims description 70
- 102000004020 Oxygenases Human genes 0.000 title claims description 70
- 108020004414 DNA Proteins 0.000 title claims description 62
- 238000000034 method Methods 0.000 title claims description 54
- 230000009261 transgenic effect Effects 0.000 title claims description 35
- 239000000463 material Substances 0.000 title claims description 18
- 102000053602 DNA Human genes 0.000 title claims description 16
- 238000010276 construction Methods 0.000 title claims description 13
- 238000012216 screening Methods 0.000 title claims description 9
- 238000005202 decontamination Methods 0.000 title claims description 5
- 230000003588 decontaminative effect Effects 0.000 title claims description 5
- 230000000593 degrading effect Effects 0.000 title claims description 4
- 241000196324 Embryophyta Species 0.000 claims description 160
- 230000014509 gene expression Effects 0.000 claims description 25
- FKIKPQHMWFZFEB-UHFFFAOYSA-N 3,6-dichloro-2-hydroxybenzoic acid Chemical compound OC(=O)C1=C(O)C(Cl)=CC=C1Cl FKIKPQHMWFZFEB-UHFFFAOYSA-N 0.000 claims description 19
- 238000006731 degradation reaction Methods 0.000 claims description 19
- 230000015556 catabolic process Effects 0.000 claims description 18
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 14
- 239000013598 vector Substances 0.000 claims description 14
- 244000005700 microbiome Species 0.000 claims description 11
- 239000002773 nucleotide Substances 0.000 claims description 9
- 125000003729 nucleotide group Chemical group 0.000 claims description 9
- 240000008042 Zea mays Species 0.000 claims description 8
- 230000003647 oxidation Effects 0.000 claims description 8
- 238000007254 oxidation reaction Methods 0.000 claims description 8
- 244000068988 Glycine max Species 0.000 claims description 7
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 7
- 230000012010 growth Effects 0.000 claims description 7
- 240000004713 Pisum sativum Species 0.000 claims description 6
- 235000010582 Pisum sativum Nutrition 0.000 claims description 6
- 239000012634 fragment Substances 0.000 claims description 6
- BKWBIMSGEOYWCJ-UHFFFAOYSA-L iron;iron(2+);sulfanide Chemical compound [SH-].[SH-].[Fe].[Fe+2] BKWBIMSGEOYWCJ-UHFFFAOYSA-L 0.000 claims description 6
- 235000010469 Glycine max Nutrition 0.000 claims description 5
- 235000013399 edible fruits Nutrition 0.000 claims description 5
- 244000003416 Asparagus officinalis Species 0.000 claims description 4
- 235000005340 Asparagus officinalis Nutrition 0.000 claims description 4
- 241000219310 Beta vulgaris subsp. vulgaris Species 0.000 claims description 4
- 229920000742 Cotton Polymers 0.000 claims description 4
- 244000299507 Gossypium hirsutum Species 0.000 claims description 4
- 241000208818 Helianthus Species 0.000 claims description 4
- 235000003222 Helianthus annuus Nutrition 0.000 claims description 4
- 235000007688 Lycopersicon esculentum Nutrition 0.000 claims description 4
- 244000061176 Nicotiana tabacum Species 0.000 claims description 4
- 235000002637 Nicotiana tabacum Nutrition 0.000 claims description 4
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 4
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 4
- 240000003768 Solanum lycopersicum Species 0.000 claims description 4
- 244000061456 Solanum tuberosum Species 0.000 claims description 4
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 4
- 240000006394 Sorghum bicolor Species 0.000 claims description 4
- 235000011684 Sorghum saccharatum Nutrition 0.000 claims description 4
- 235000021536 Sugar beet Nutrition 0.000 claims description 4
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 4
- 235000005822 corn Nutrition 0.000 claims description 4
- 239000001963 growth medium Substances 0.000 claims description 4
- 235000012015 potatoes Nutrition 0.000 claims description 4
- 244000025254 Cannabis sativa Species 0.000 claims description 3
- 230000001131 transforming effect Effects 0.000 claims description 3
- 235000013339 cereals Nutrition 0.000 claims description 2
- 125000003275 alpha amino acid group Chemical group 0.000 claims 6
- 108090000623 proteins and genes Proteins 0.000 description 111
- 210000004027 cell Anatomy 0.000 description 77
- 102000004169 proteins and genes Human genes 0.000 description 74
- 235000018102 proteins Nutrition 0.000 description 70
- 230000000694 effects Effects 0.000 description 68
- 239000000499 gel Substances 0.000 description 44
- 102000004190 Enzymes Human genes 0.000 description 42
- 108090000790 Enzymes Proteins 0.000 description 42
- 229940088598 enzyme Drugs 0.000 description 42
- 239000000872 buffer Substances 0.000 description 39
- 102000004316 Oxidoreductases Human genes 0.000 description 38
- 108090000854 Oxidoreductases Proteins 0.000 description 38
- 150000001413 amino acids Chemical group 0.000 description 33
- 238000006243 chemical reaction Methods 0.000 description 32
- 108010056846 dicamba O-demethylase Proteins 0.000 description 32
- 108010074122 Ferredoxins Proteins 0.000 description 31
- 238000001155 isoelectric focusing Methods 0.000 description 28
- 241000122973 Stenotrophomonas maltophilia Species 0.000 description 22
- 239000004009 herbicide Substances 0.000 description 21
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 description 21
- 238000000746 purification Methods 0.000 description 21
- 101000623895 Bos taurus Mucin-15 Proteins 0.000 description 19
- CCBICDLNWJRFPO-UHFFFAOYSA-N 2,6-dichloroindophenol Chemical compound C1=CC(O)=CC=C1N=C1C=C(Cl)C(=O)C(Cl)=C1 CCBICDLNWJRFPO-UHFFFAOYSA-N 0.000 description 16
- 239000000203 mixture Substances 0.000 description 16
- 241000894006 Bacteria Species 0.000 description 15
- 102100034289 Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 Human genes 0.000 description 15
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 15
- 101000641031 Homo sapiens Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 Proteins 0.000 description 15
- 239000003550 marker Substances 0.000 description 14
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 13
- 230000002363 herbicidal effect Effects 0.000 description 12
- 230000009466 transformation Effects 0.000 description 12
- 229920002684 Sepharose Polymers 0.000 description 11
- 235000001014 amino acid Nutrition 0.000 description 11
- 238000003556 assay Methods 0.000 description 11
- 239000011541 reaction mixture Substances 0.000 description 11
- 239000000523 sample Substances 0.000 description 11
- 238000004809 thin layer chromatography Methods 0.000 description 11
- 108010044672 O-Demethylating Oxidoreductases Proteins 0.000 description 10
- ROOXNKNUYICQNP-UHFFFAOYSA-N ammonium persulfate Chemical compound [NH4+].[NH4+].[O-]S(=O)(=O)OOS([O-])(=O)=O ROOXNKNUYICQNP-UHFFFAOYSA-N 0.000 description 10
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 10
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 10
- 235000011130 ammonium sulphate Nutrition 0.000 description 10
- 230000001580 bacterial effect Effects 0.000 description 10
- 238000001962 electrophoresis Methods 0.000 description 10
- 238000000926 separation method Methods 0.000 description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 10
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 9
- 239000012614 Q-Sepharose Substances 0.000 description 9
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 9
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 9
- 238000004587 chromatography analysis Methods 0.000 description 9
- 229910052760 oxygen Inorganic materials 0.000 description 9
- 239000001301 oxygen Substances 0.000 description 9
- 239000000758 substrate Substances 0.000 description 9
- 108020004705 Codon Proteins 0.000 description 8
- 239000013592 cell lysate Substances 0.000 description 8
- 210000003763 chloroplast Anatomy 0.000 description 8
- 239000007799 cork Substances 0.000 description 8
- 238000004519 manufacturing process Methods 0.000 description 8
- 229920002401 polyacrylamide Polymers 0.000 description 8
- 241000206602 Eukaryota Species 0.000 description 7
- 230000001747 exhibiting effect Effects 0.000 description 7
- 235000019162 flavin adenine dinucleotide Nutrition 0.000 description 7
- 239000011714 flavin adenine dinucleotide Substances 0.000 description 7
- VWWQXMAJTJZDQX-UYBVJOGSSA-N flavin adenine dinucleotide Chemical compound C1=NC2=C(N)N=CN=C2N1[C@@H]([C@H](O)[C@@H]1O)O[C@@H]1CO[P@](O)(=O)O[P@@](O)(=O)OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C2=NC(=O)NC(=O)C2=NC2=C1C=C(C)C(C)=C2 VWWQXMAJTJZDQX-UYBVJOGSSA-N 0.000 description 7
- 229940093632 flavin-adenine dinucleotide Drugs 0.000 description 7
- 238000002955 isolation Methods 0.000 description 7
- 238000002360 preparation method Methods 0.000 description 7
- 238000012163 sequencing technique Methods 0.000 description 7
- 239000002689 soil Substances 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 6
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- 102100030497 Cytochrome c Human genes 0.000 description 6
- 108010075031 Cytochromes c Proteins 0.000 description 6
- 108700028146 Genetic Enhancer Elements Proteins 0.000 description 6
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 6
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 238000004458 analytical method Methods 0.000 description 6
- 230000001939 inductive effect Effects 0.000 description 6
- 239000002609 medium Substances 0.000 description 6
- 238000010369 molecular cloning Methods 0.000 description 6
- 239000008363 phosphate buffer Substances 0.000 description 6
- 238000013518 transcription Methods 0.000 description 6
- 230000035897 transcription Effects 0.000 description 6
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 5
- 108010028143 Dioxygenases Proteins 0.000 description 5
- 102000016680 Dioxygenases Human genes 0.000 description 5
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 5
- 239000007983 Tris buffer Substances 0.000 description 5
- 229910001870 ammonium persulfate Inorganic materials 0.000 description 5
- 230000015572 biosynthetic process Effects 0.000 description 5
- 238000005119 centrifugation Methods 0.000 description 5
- 238000012512 characterization method Methods 0.000 description 5
- 230000001419 dependent effect Effects 0.000 description 5
- 238000001514 detection method Methods 0.000 description 5
- 238000010828 elution Methods 0.000 description 5
- 230000002255 enzymatic effect Effects 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 238000000338 in vitro Methods 0.000 description 5
- 239000002751 oligonucleotide probe Substances 0.000 description 5
- 230000009467 reduction Effects 0.000 description 5
- 241000589158 Agrobacterium Species 0.000 description 4
- 241000193403 Clostridium Species 0.000 description 4
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 4
- 108010043121 Green Fluorescent Proteins Proteins 0.000 description 4
- 102000004144 Green Fluorescent Proteins Human genes 0.000 description 4
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 4
- KWYHDKDOAIKMQN-UHFFFAOYSA-N N,N,N',N'-tetramethylethylenediamine Chemical compound CN(C)CCN(C)C KWYHDKDOAIKMQN-UHFFFAOYSA-N 0.000 description 4
- 125000001429 N-terminal alpha-amino-acid group Chemical group 0.000 description 4
- 241000589776 Pseudomonas putida Species 0.000 description 4
- 108091007187 Reductases Proteins 0.000 description 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 4
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 4
- 241000700605 Viruses Species 0.000 description 4
- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 description 4
- 239000007795 chemical reaction product Substances 0.000 description 4
- 238000004440 column chromatography Methods 0.000 description 4
- 235000013305 food Nutrition 0.000 description 4
- 239000005090 green fluorescent protein Substances 0.000 description 4
- 150000003278 haem Chemical group 0.000 description 4
- 230000001965 increasing effect Effects 0.000 description 4
- 108091027963 non-coding RNA Proteins 0.000 description 4
- 102000042567 non-coding RNA Human genes 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 210000001519 tissue Anatomy 0.000 description 4
- QFVHZQCOUORWEI-UHFFFAOYSA-N 4-[(4-anilino-5-sulfonaphthalen-1-yl)diazenyl]-5-hydroxynaphthalene-2,7-disulfonic acid Chemical compound C=12C(O)=CC(S(O)(=O)=O)=CC2=CC(S(O)(=O)=O)=CC=1N=NC(C1=CC=CC(=C11)S(O)(=O)=O)=CC=C1NC1=CC=CC=C1 QFVHZQCOUORWEI-UHFFFAOYSA-N 0.000 description 3
- 229920000936 Agarose Polymers 0.000 description 3
- 241000589155 Agrobacterium tumefaciens Species 0.000 description 3
- 241000701489 Cauliflower mosaic virus Species 0.000 description 3
- 239000004471 Glycine Substances 0.000 description 3
- ACFIXJIJDZMPPO-NNYOXOHSSA-N NADPH Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](OP(O)(O)=O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 ACFIXJIJDZMPPO-NNYOXOHSSA-N 0.000 description 3
- 241000276498 Pollachius virens Species 0.000 description 3
- 108091036066 Three prime untranslated region Proteins 0.000 description 3
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 3
- 238000010520 demethylation reaction Methods 0.000 description 3
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000013604 expression vector Substances 0.000 description 3
- 229940013640 flavin mononucleotide Drugs 0.000 description 3
- 239000011768 flavin mononucleotide Substances 0.000 description 3
- FVTCRASFADXXNN-SCRDCRAPSA-N flavin mononucleotide Chemical compound OP(=O)(O)OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O FVTCRASFADXXNN-SCRDCRAPSA-N 0.000 description 3
- FVTCRASFADXXNN-UHFFFAOYSA-N flavin mononucleotide Natural products OP(=O)(O)OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O FVTCRASFADXXNN-UHFFFAOYSA-N 0.000 description 3
- 238000002523 gelfiltration Methods 0.000 description 3
- 230000005764 inhibitory process Effects 0.000 description 3
- 235000009973 maize Nutrition 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 108020004999 messenger RNA Proteins 0.000 description 3
- 239000002207 metabolite Substances 0.000 description 3
- 238000013508 migration Methods 0.000 description 3
- 230000005012 migration Effects 0.000 description 3
- 238000002703 mutagenesis Methods 0.000 description 3
- 231100000350 mutagenesis Toxicity 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 239000013612 plasmid Substances 0.000 description 3
- 229920002981 polyvinylidene fluoride Polymers 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 230000002285 radioactive effect Effects 0.000 description 3
- 235000019231 riboflavin-5'-phosphate Nutrition 0.000 description 3
- 244000000000 soil microbiome Species 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 238000003786 synthesis reaction Methods 0.000 description 3
- 230000008685 targeting Effects 0.000 description 3
- 238000013519 translation Methods 0.000 description 3
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 3
- PAJPWUMXBYXFCZ-UHFFFAOYSA-N 1-aminocyclopropanecarboxylic acid Chemical compound OC(=O)C1(N)CC1 PAJPWUMXBYXFCZ-UHFFFAOYSA-N 0.000 description 2
- 239000005631 2,4-Dichlorophenoxyacetic acid Substances 0.000 description 2
- UPMXNNIRAGDFEH-UHFFFAOYSA-N 3,5-dibromo-4-hydroxybenzonitrile Chemical compound OC1=C(Br)C=C(C#N)C=C1Br UPMXNNIRAGDFEH-UHFFFAOYSA-N 0.000 description 2
- 241000589156 Agrobacterium rhizogenes Species 0.000 description 2
- 108010065511 Amylases Proteins 0.000 description 2
- 241000228245 Aspergillus niger Species 0.000 description 2
- 108091005658 Basic proteases Proteins 0.000 description 2
- 239000005711 Benzoic acid Substances 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- 102100024633 Carbonic anhydrase 2 Human genes 0.000 description 2
- 101710167917 Carbonic anhydrase 2 Proteins 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- 238000004435 EPR spectroscopy Methods 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 2
- 241000701484 Figwort mosaic virus Species 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 108010060309 Glucuronidase Proteins 0.000 description 2
- 102000053187 Glucuronidase Human genes 0.000 description 2
- 239000005562 Glyphosate Substances 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- 108010074633 Mixed Function Oxygenases Proteins 0.000 description 2
- 102000008109 Mixed Function Oxygenases Human genes 0.000 description 2
- 108010014251 Muramidase Proteins 0.000 description 2
- 102000016943 Muramidase Human genes 0.000 description 2
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 2
- 239000004677 Nylon Substances 0.000 description 2
- 239000002033 PVDF binder Substances 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- 108010076504 Protein Sorting Signals Proteins 0.000 description 2
- 241000589614 Pseudomonas stutzeri Species 0.000 description 2
- 101710082095 Putative ferredoxin Proteins 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 150000007513 acids Chemical class 0.000 description 2
- 239000011543 agarose gel Substances 0.000 description 2
- 108090000637 alpha-Amylases Proteins 0.000 description 2
- 102000004139 alpha-Amylases Human genes 0.000 description 2
- 229940024171 alpha-amylase Drugs 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 230000000692 anti-sense effect Effects 0.000 description 2
- 229940088710 antibiotic agent Drugs 0.000 description 2
- 235000010233 benzoic acid Nutrition 0.000 description 2
- 239000001045 blue dye Substances 0.000 description 2
- 229940098773 bovine serum albumin Drugs 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 239000012468 concentrated sample Substances 0.000 description 2
- NKLPQNGYXWVELD-UHFFFAOYSA-M coomassie brilliant blue Chemical compound [Na+].C1=CC(OCC)=CC=C1NC1=CC=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=2C=CC(=CC=2)N(CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=C1 NKLPQNGYXWVELD-UHFFFAOYSA-M 0.000 description 2
- -1 cosmids Substances 0.000 description 2
- 125000000151 cysteine group Chemical group N[C@@H](CS)C(=O)* 0.000 description 2
- 239000007857 degradation product Substances 0.000 description 2
- 239000000975 dye Substances 0.000 description 2
- 239000003623 enhancer Substances 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000001952 enzyme assay Methods 0.000 description 2
- 230000007717 exclusion Effects 0.000 description 2
- 238000001502 gel electrophoresis Methods 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- XDDAORKBJWWYJS-UHFFFAOYSA-N glyphosate Chemical compound OC(=O)CNCP(O)(O)=O XDDAORKBJWWYJS-UHFFFAOYSA-N 0.000 description 2
- 229940097068 glyphosate Drugs 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 229960000318 kanamycin Drugs 0.000 description 2
- 229930027917 kanamycin Natural products 0.000 description 2
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 2
- 229930182823 kanamycin A Natural products 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000004811 liquid chromatography Methods 0.000 description 2
- 239000006166 lysate Substances 0.000 description 2
- 239000004325 lysozyme Substances 0.000 description 2
- 229960000274 lysozyme Drugs 0.000 description 2
- 235000010335 lysozyme Nutrition 0.000 description 2
- 229910001629 magnesium chloride Inorganic materials 0.000 description 2
- 210000004962 mammalian cell Anatomy 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 230000001404 mediated effect Effects 0.000 description 2
- 238000002844 melting Methods 0.000 description 2
- 229920001778 nylon Polymers 0.000 description 2
- 230000037361 pathway Effects 0.000 description 2
- 239000008057 potassium phosphate buffer Substances 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 210000001938 protoplast Anatomy 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 239000012146 running buffer Substances 0.000 description 2
- 239000012723 sample buffer Substances 0.000 description 2
- 238000001542 size-exclusion chromatography Methods 0.000 description 2
- 238000000527 sonication Methods 0.000 description 2
- 230000005030 transcription termination Effects 0.000 description 2
- 230000002103 transcriptional effect Effects 0.000 description 2
- 230000014621 translational initiation Effects 0.000 description 2
- 241000701447 unidentified baculovirus Species 0.000 description 2
- 238000011144 upstream manufacturing Methods 0.000 description 2
- KBBJNRLWAGIQLW-BYPYZUCNSA-N (2r)-2-(propanoylamino)-3-sulfanylpropanoic acid Chemical group CCC(=O)N[C@@H](CS)C(O)=O KBBJNRLWAGIQLW-BYPYZUCNSA-N 0.000 description 1
- 108091000130 1-aminocyclopropane-1-carboxylate deaminase Proteins 0.000 description 1
- 108010010888 1-aminocyclopropane-1-carboxylic acid oxidase Proteins 0.000 description 1
- HXKWSTRRCHTUEC-UHFFFAOYSA-N 2,4-Dichlorophenoxyaceticacid Chemical compound OC(=O)C(Cl)OC1=CC=C(Cl)C=C1 HXKWSTRRCHTUEC-UHFFFAOYSA-N 0.000 description 1
- IAJOBQBIJHVGMQ-UHFFFAOYSA-N 2-amino-4-[hydroxy(methyl)phosphoryl]butanoic acid Chemical compound CP(O)(=O)CCC(N)C(O)=O IAJOBQBIJHVGMQ-UHFFFAOYSA-N 0.000 description 1
- ZBMRKNMTMPPMMK-UHFFFAOYSA-N 2-amino-4-[hydroxy(methyl)phosphoryl]butanoic acid;azane Chemical compound [NH4+].CP(O)(=O)CCC(N)C([O-])=O ZBMRKNMTMPPMMK-UHFFFAOYSA-N 0.000 description 1
- 108010087036 2-oxo-1,2-dihydroquinoline 8-monooxygenase Proteins 0.000 description 1
- LULAYUGMBFYYEX-UHFFFAOYSA-M 3-chlorobenzoate Chemical compound [O-]C(=O)C1=CC=CC(Cl)=C1 LULAYUGMBFYYEX-UHFFFAOYSA-M 0.000 description 1
- 108010020183 3-phosphoshikimate 1-carboxyvinyltransferase Proteins 0.000 description 1
- 108010051365 4-chlorophenylacetate 3,4-dioxygenase Proteins 0.000 description 1
- 108010050051 4-sulfobenzoate 3,4-dioxygenase Proteins 0.000 description 1
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
- 108010021809 Alcohol dehydrogenase Proteins 0.000 description 1
- 102100034042 Alcohol dehydrogenase 1C Human genes 0.000 description 1
- 239000004382 Amylase Substances 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 102000004580 Aspartic Acid Proteases Human genes 0.000 description 1
- 108010017640 Aspartic Acid Proteases Proteins 0.000 description 1
- 240000006439 Aspergillus oryzae Species 0.000 description 1
- 241001203868 Autographa californica Species 0.000 description 1
- 241000589151 Azotobacter Species 0.000 description 1
- 241000193744 Bacillus amyloliquefaciens Species 0.000 description 1
- 108010029675 Bacillus licheniformis alpha-amylase Proteins 0.000 description 1
- 241000194103 Bacillus pumilus Species 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- KHBQMWCZKVMBLN-UHFFFAOYSA-N Benzenesulfonamide Chemical compound NS(=O)(=O)C1=CC=CC=C1 KHBQMWCZKVMBLN-UHFFFAOYSA-N 0.000 description 1
- 102100026189 Beta-galactosidase Human genes 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 101000800130 Bos taurus Thyroglobulin Proteins 0.000 description 1
- 240000002791 Brassica napus Species 0.000 description 1
- 235000011293 Brassica napus Nutrition 0.000 description 1
- 206010006187 Breast cancer Diseases 0.000 description 1
- 239000005489 Bromoxynil Substances 0.000 description 1
- 101100280051 Brucella abortus biovar 1 (strain 9-941) eryH gene Proteins 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 101710132601 Capsid protein Proteins 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 108010033547 Carbonic Anhydrase I Proteins 0.000 description 1
- 102100025518 Carbonic anhydrase 1 Human genes 0.000 description 1
- 102000003846 Carbonic anhydrases Human genes 0.000 description 1
- 108090000209 Carbonic anhydrases Proteins 0.000 description 1
- 108010035563 Chloramphenicol O-acetyltransferase Proteins 0.000 description 1
- 241000701248 Chlorella virus Species 0.000 description 1
- 241000193469 Clostridium pasteurianum Species 0.000 description 1
- 101710094648 Coat protein Proteins 0.000 description 1
- 108091026890 Coding region Proteins 0.000 description 1
- 241000589518 Comamonas testosteroni Species 0.000 description 1
- 238000011537 Coomassie blue staining Methods 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 101000796894 Coturnix japonica Alcohol dehydrogenase 1 Proteins 0.000 description 1
- 241000724252 Cucumber mosaic virus Species 0.000 description 1
- PJWWRFATQTVXHA-UHFFFAOYSA-N Cyclohexylaminopropanesulfonic acid Chemical compound OS(=O)(=O)CCCNC1CCCCC1 PJWWRFATQTVXHA-UHFFFAOYSA-N 0.000 description 1
- 108010066133 D-octopine dehydrogenase Proteins 0.000 description 1
- 238000001712 DNA sequencing Methods 0.000 description 1
- 108010054576 Deoxyribonuclease EcoRI Proteins 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 1
- 241001269524 Dura Species 0.000 description 1
- 101150048726 E9 gene Proteins 0.000 description 1
- YQYJSBFKSSDGFO-UHFFFAOYSA-N Epihygromycin Natural products OC1C(O)C(C(=O)C)OC1OC(C(=C1)O)=CC=C1C=C(C)C(=O)NC1C(O)C(O)C2OCOC2C1O YQYJSBFKSSDGFO-UHFFFAOYSA-N 0.000 description 1
- 101000635852 Equus caballus Myoglobin Proteins 0.000 description 1
- 241000701959 Escherichia virus Lambda Species 0.000 description 1
- 102000003983 Flavoproteins Human genes 0.000 description 1
- 108010057573 Flavoproteins Proteins 0.000 description 1
- 101000609762 Gallus gallus Ovalbumin Proteins 0.000 description 1
- 229920002148 Gellan gum Polymers 0.000 description 1
- 229930182566 Gentamicin Natural products 0.000 description 1
- CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 description 1
- 241000193385 Geobacillus stearothermophilus Species 0.000 description 1
- 108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 description 1
- 102100022624 Glucoamylase Human genes 0.000 description 1
- 239000005561 Glufosinate Substances 0.000 description 1
- 108010063907 Glutathione Reductase Proteins 0.000 description 1
- 102100036442 Glutathione reductase, mitochondrial Human genes 0.000 description 1
- 108010033040 Histones Proteins 0.000 description 1
- 101000780463 Homo sapiens Alcohol dehydrogenase 1C Proteins 0.000 description 1
- 101000615488 Homo sapiens Methyl-CpG-binding domain protein 2 Proteins 0.000 description 1
- 101000801742 Homo sapiens Triosephosphate isomerase Proteins 0.000 description 1
- 241000701109 Human adenovirus 2 Species 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 101150017040 I gene Proteins 0.000 description 1
- 108010025815 Kanamycin Kinase Proteins 0.000 description 1
- 108091026898 Leader sequence (mRNA) Proteins 0.000 description 1
- 108030005702 Leghemoglobin reductases Proteins 0.000 description 1
- 108090001060 Lipase Proteins 0.000 description 1
- 102000004882 Lipase Human genes 0.000 description 1
- 239000004367 Lipase Substances 0.000 description 1
- 108060001084 Luciferase Proteins 0.000 description 1
- 239000005089 Luciferase Substances 0.000 description 1
- 241000219745 Lupinus Species 0.000 description 1
- 101710125418 Major capsid protein Proteins 0.000 description 1
- 108090000157 Metallothionein Proteins 0.000 description 1
- 108060004795 Methyltransferase Proteins 0.000 description 1
- 241001529936 Murinae Species 0.000 description 1
- 101000966481 Mus musculus Dihydrofolate reductase Proteins 0.000 description 1
- 101100235161 Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) lerI gene Proteins 0.000 description 1
- 102000003505 Myosin Human genes 0.000 description 1
- 108060008487 Myosin Proteins 0.000 description 1
- FBWADIKARMIWNM-UHFFFAOYSA-N N-3,5-dichloro-4-hydroxyphenyl-1,4-benzoquinone imine Chemical compound C1=C(Cl)C(O)=C(Cl)C=C1N=C1C=CC(=O)C=C1 FBWADIKARMIWNM-UHFFFAOYSA-N 0.000 description 1
- BAWFJGJZGIEFAR-NNYOXOHSSA-N NAD zwitterion Chemical group NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 BAWFJGJZGIEFAR-NNYOXOHSSA-N 0.000 description 1
- 229930193140 Neomycin Natural products 0.000 description 1
- 101710141454 Nucleoprotein Proteins 0.000 description 1
- 108010058846 Ovalbumin Proteins 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 102000004270 Peptidyl-Dipeptidase A Human genes 0.000 description 1
- 108090000882 Peptidyl-Dipeptidase A Proteins 0.000 description 1
- 101710163504 Phaseolin Proteins 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-L Phosphate ion(2-) Chemical compound OP([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-L 0.000 description 1
- 108010065081 Phosphorylase b Proteins 0.000 description 1
- 241000209504 Poaceae Species 0.000 description 1
- 101710182846 Polyhedrin Proteins 0.000 description 1
- 239000004372 Polyvinyl alcohol Substances 0.000 description 1
- 101710083689 Probable capsid protein Proteins 0.000 description 1
- 241000589630 Pseudomonas pseudoalcaligenes Species 0.000 description 1
- 241000589774 Pseudomonas sp. Species 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 241000235402 Rhizomucor Species 0.000 description 1
- 241000235403 Rhizomucor miehei Species 0.000 description 1
- 241000191023 Rhodobacter capsulatus Species 0.000 description 1
- 241000190984 Rhodospirillum rubrum Species 0.000 description 1
- 241000714474 Rous sarcoma virus Species 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 241001135759 Sphingomonas sp. Species 0.000 description 1
- 108091081024 Start codon Proteins 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
- 239000005864 Sulphur Substances 0.000 description 1
- 241000723873 Tobacco mosaic virus Species 0.000 description 1
- 102100033598 Triosephosphate isomerase Human genes 0.000 description 1
- 102000044159 Ubiquitin Human genes 0.000 description 1
- 108090000848 Ubiquitin Proteins 0.000 description 1
- 235000007244 Zea mays Nutrition 0.000 description 1
- 241001300270 Zingel streber Species 0.000 description 1
- 230000001594 aberrant effect Effects 0.000 description 1
- 108010048241 acetamidase Proteins 0.000 description 1
- MBRZVWSXTLBVNY-UHFFFAOYSA-N acetic acid;chloroform;ethanol Chemical compound CCO.CC(O)=O.ClC(Cl)Cl MBRZVWSXTLBVNY-UHFFFAOYSA-N 0.000 description 1
- 108020002494 acetyltransferase Proteins 0.000 description 1
- 102000005421 acetyltransferase Human genes 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 230000009418 agronomic effect Effects 0.000 description 1
- 230000029936 alkylation Effects 0.000 description 1
- 238000005804 alkylation reaction Methods 0.000 description 1
- 125000000539 amino acid group Chemical group 0.000 description 1
- 239000001166 ammonium sulphate Substances 0.000 description 1
- 229960000723 ampicillin Drugs 0.000 description 1
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 238000005349 anion exchange Methods 0.000 description 1
- 235000010208 anthocyanin Nutrition 0.000 description 1
- 229930002877 anthocyanin Natural products 0.000 description 1
- 239000004410 anthocyanin Substances 0.000 description 1
- 150000004636 anthocyanins Chemical class 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 239000012298 atmosphere Substances 0.000 description 1
- 235000020054 awamori Nutrition 0.000 description 1
- 125000001797 benzyl group Chemical group [H]C1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])* 0.000 description 1
- 108010005774 beta-Galactosidase Proteins 0.000 description 1
- ACFIXJIJDZMPPO-UHFFFAOYSA-N beta-NADPH Natural products C1=CCC(C(=O)N)=CN1C1C(O)C(O)C(COP(O)(=O)OP(O)(=O)OCC2C(C(OP(O)(O)=O)C(O2)N2C3=NC=NC(N)=C3N=C2)O)O1 ACFIXJIJDZMPPO-UHFFFAOYSA-N 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 108010083912 bleomycin N-acetyltransferase Proteins 0.000 description 1
- UDSAIICHUKSCKT-UHFFFAOYSA-N bromophenol blue Chemical compound C1=C(Br)C(O)=C(Br)C=C1C1(C=2C=C(Br)C(O)=C(Br)C=2)C2=CC=CC=C2S(=O)(=O)O1 UDSAIICHUKSCKT-UHFFFAOYSA-N 0.000 description 1
- 230000005587 bubbling Effects 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 235000011148 calcium chloride Nutrition 0.000 description 1
- 238000005251 capillar electrophoresis Methods 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000010307 cell transformation Effects 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 229960005091 chloramphenicol Drugs 0.000 description 1
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- YKCWQPZFAFZLBI-UHFFFAOYSA-N cibacron blue Chemical compound C1=2C(=O)C3=CC=CC=C3C(=O)C=2C(N)=C(S(O)(=O)=O)C=C1NC(C=C1S(O)(=O)=O)=CC=C1NC(N=1)=NC(Cl)=NC=1NC1=CC=CC=C1S(O)(=O)=O YKCWQPZFAFZLBI-UHFFFAOYSA-N 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- FDJOLVPMNUYSCM-WZHZPDAFSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+3].N#[C-].N([C@@H]([C@]1(C)[N-]\C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C(\C)/C1=N/C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C\C1=N\C([C@H](C1(C)C)CCC(N)=O)=C/1C)[C@@H]2CC(N)=O)=C\1[C@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]1[C@@H](O)[C@@H](N2C3=CC(C)=C(C)C=C3N=C2)O[C@@H]1CO FDJOLVPMNUYSCM-WZHZPDAFSA-L 0.000 description 1
- 239000002299 complementary DNA Substances 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 238000004042 decolorization Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 239000001064 degrader Substances 0.000 description 1
- 230000001066 destructive effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 108010069652 dibenzofuran 4,4a-dioxygenase Proteins 0.000 description 1
- 239000000539 dimer Substances 0.000 description 1
- 229910001882 dioxygen Inorganic materials 0.000 description 1
- 238000004520 electroporation Methods 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 210000002257 embryonic structure Anatomy 0.000 description 1
- 210000003527 eukaryotic cell Anatomy 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000011790 ferrous sulphate Substances 0.000 description 1
- 235000003891 ferrous sulphate Nutrition 0.000 description 1
- 238000001943 fluorescence-activated cell sorting Methods 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 108010074605 gamma-Globulins Proteins 0.000 description 1
- 238000002290 gas chromatography-mass spectrometry Methods 0.000 description 1
- 229960002518 gentamicin Drugs 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 108010061330 glucan 1,4-alpha-maltohydrolase Proteins 0.000 description 1
- 239000003862 glucocorticoid Substances 0.000 description 1
- 230000002414 glycolytic effect Effects 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 239000000710 homodimer Substances 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- RSAZYXZUJROYKR-UHFFFAOYSA-N indophenol Chemical compound C1=CC(O)=CC=C1N=C1C=CC(=O)C=C1 RSAZYXZUJROYKR-UHFFFAOYSA-N 0.000 description 1
- 239000000411 inducer Substances 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 1
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 1
- 238000012933 kinetic analysis Methods 0.000 description 1
- 235000019421 lipase Nutrition 0.000 description 1
- 239000002502 liposome Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- LULAYUGMBFYYEX-UHFFFAOYSA-N metachloroperbenzoic acid Natural products OC(=O)C1=CC=CC(Cl)=C1 LULAYUGMBFYYEX-UHFFFAOYSA-N 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000000329 molecular dynamics simulation Methods 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 210000004897 n-terminal region Anatomy 0.000 description 1
- 229960004927 neomycin Drugs 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 108010058731 nopaline synthase Proteins 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 230000031787 nutrient reservoir activity Effects 0.000 description 1
- 238000005580 one pot reaction Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 229940092253 ovalbumin Drugs 0.000 description 1
- 125000004430 oxygen atom Chemical group O* 0.000 description 1
- 239000006174 pH buffer Substances 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000002085 persistent effect Effects 0.000 description 1
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 108010088800 phthalate dioxygenase Proteins 0.000 description 1
- 230000019612 pigmentation Effects 0.000 description 1
- 230000008488 polyadenylation Effects 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 108010055896 polyornithine Proteins 0.000 description 1
- 229920002451 polyvinyl alcohol Polymers 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 230000007026 protein scission Effects 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 238000005067 remediation Methods 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 230000035040 seed growth Effects 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 238000002741 site-directed mutagenesis Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 210000001082 somatic cell Anatomy 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 239000003270 steroid hormone Substances 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 108010008664 streptomycin 3''-kinase Proteins 0.000 description 1
- OBTWBSRJZRCYQV-UHFFFAOYSA-N sulfuryl difluoride Chemical compound FS(F)(=O)=O OBTWBSRJZRCYQV-UHFFFAOYSA-N 0.000 description 1
- 235000011149 sulphuric acid Nutrition 0.000 description 1
- 238000004114 suspension culture Methods 0.000 description 1
- 230000006032 tissue transformation Effects 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 101150080369 tpiA gene Proteins 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 150000003641 trioses Chemical class 0.000 description 1
- 108010070467 vanillate O-demethylase Proteins 0.000 description 1
- 239000013603 viral vector Substances 0.000 description 1
- 239000011800 void material Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8259—Phytoremediation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US4294197P | 1997-04-04 | 1997-04-04 | |
US4266697P | 1997-04-04 | 1997-04-04 | |
PCT/US1998/006589 WO1998045424A1 (fr) | 1997-04-04 | 1998-04-03 | Methodes et materiels pour produire et utiliser des organismes transgeniques degradant dicamba |
Publications (3)
Publication Number | Publication Date |
---|---|
NO994795D0 NO994795D0 (no) | 1999-10-01 |
NO994795L NO994795L (no) | 1999-12-01 |
NO324769B1 true NO324769B1 (no) | 2007-12-10 |
Family
ID=26719500
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
NO19994795A NO324769B1 (no) | 1997-04-04 | 1999-10-01 | Isolert DNA molekyl, DNA konstruksjon, renset dicambadegraderende oksygenase, transgen vertscelle og plante, fremgangsmate for dekontaminering av et materiale inneholdende dicamba, for selektering av transformerte planteceller og for selektering, eller screening for, transformerte vertsceller, intakte organismer og deler av organismer. |
Country Status (9)
Country | Link |
---|---|
EP (2) | EP2325308A3 (fr) |
JP (3) | JP2001519663A (fr) |
KR (1) | KR100558838B1 (fr) |
AU (1) | AU741974B2 (fr) |
CA (1) | CA2285676C (fr) |
NO (1) | NO324769B1 (fr) |
NZ (1) | NZ338093A (fr) |
RU (1) | RU2226215C2 (fr) |
WO (1) | WO1998045424A1 (fr) |
Families Citing this family (23)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7105724B2 (en) | 1997-04-04 | 2006-09-12 | Board Of Regents Of University Of Nebraska | Methods and materials for making and using transgenic dicamba-degrading organisms |
JP2005506043A (ja) * | 2001-02-28 | 2005-03-03 | ボード・オブ・リージェンツ・オブ・ザ・ユニバーシティ・オブ・ネブラスカ | 遺伝子導入ジカンバ分解性生物体を作製及び使用するための方法及び材料 |
US7884262B2 (en) | 2006-06-06 | 2011-02-08 | Monsanto Technology Llc | Modified DMO enzyme and methods of its use |
WO2007146678A1 (fr) | 2006-06-06 | 2007-12-21 | Monsanto Technology Llc | Procédé de sélection de cellules transformées |
US7855326B2 (en) | 2006-06-06 | 2010-12-21 | Monsanto Technology Llc | Methods for weed control using plants having dicamba-degrading enzymatic activity |
US8207092B2 (en) | 2006-10-16 | 2012-06-26 | Monsanto Technology Llc | Methods and compositions for improving plant health |
US7939721B2 (en) * | 2006-10-25 | 2011-05-10 | Monsanto Technology Llc | Cropping systems for managing weeds |
US7838729B2 (en) | 2007-02-26 | 2010-11-23 | Monsanto Technology Llc | Chloroplast transit peptides for efficient targeting of DMO and uses thereof |
ES2866126T3 (es) | 2009-09-17 | 2021-10-19 | Monsanto Technology Llc | Evento transgénico de soja MON 87708 y procedimientos de uso del mismo |
GB0920891D0 (en) | 2009-11-27 | 2010-01-13 | Syngenta Participations Ag | Herbicidal compositions |
TWI667347B (zh) | 2010-12-15 | 2019-08-01 | 瑞士商先正達合夥公司 | 大豆品種syht0h2及偵測其之組合物及方法 |
CN103597079B (zh) | 2011-03-30 | 2017-04-05 | 孟山都技术公司 | 棉花转基因事件mon88701及其使用方法 |
GB201109239D0 (en) | 2011-06-01 | 2011-07-13 | Syngenta Participations Ag | Herbicidal compositions |
PE20161403A1 (es) | 2014-03-20 | 2016-12-29 | Monsanto Technology Llc | Evento de maiz transgenico mon 87419 y metodos para su uso |
CN104630162B (zh) * | 2014-12-22 | 2017-07-25 | 北京大北农生物技术有限公司 | 除草剂耐受性蛋白质、其编码基因及用途 |
US11046969B2 (en) * | 2017-05-24 | 2021-06-29 | Epiplanta Biotech Ltd. | Transgenic plant and the method for producing the same |
US10428347B2 (en) | 2017-07-24 | 2019-10-01 | Spogen Biotech Inc. | Herbicide-detoxifying enzymes and uses thereof |
CN109971773B (zh) * | 2019-03-29 | 2022-02-22 | 南阳师范学院 | 一种编码可降解3-氯龙胆酸的龙胆酸双加氧酶DsmI的基因dsmI及其应用 |
CN111118037B (zh) * | 2019-10-14 | 2021-07-13 | 中国农业科学院生物技术研究所 | 具有除草剂麦草畏降解功能的基因dicx4及其应用 |
CN111139238B (zh) * | 2019-10-14 | 2021-08-03 | 中国农业科学院生物技术研究所 | 除草剂麦草畏的降解基因dicX3及其应用 |
CN111139248B (zh) * | 2019-10-14 | 2021-07-13 | 中国农业科学院生物技术研究所 | dicX5基因及其对除草剂的降解功能 |
CN111139249B (zh) * | 2019-10-14 | 2021-07-09 | 中国农业科学院生物技术研究所 | 运动金球菌麦草畏降解基因dicM的用途 |
CN110872591B (zh) * | 2019-10-14 | 2021-07-13 | 中国农业科学院生物技术研究所 | 除草剂麦草畏降解基因dicX1及其应用 |
Family Cites Families (35)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
NL8300698A (nl) | 1983-02-24 | 1984-09-17 | Univ Leiden | Werkwijze voor het inbouwen van vreemd dna in het genoom van tweezaadlobbige planten; agrobacterium tumefaciens bacterien en werkwijze voor het produceren daarvan; planten en plantecellen met gewijzigde genetische eigenschappen; werkwijze voor het bereiden van chemische en/of farmaceutische produkten. |
US5102796A (en) | 1983-04-15 | 1992-04-07 | Lubrizol Genetics, Inc. | Plant structural gene expression |
US5428147A (en) | 1983-04-15 | 1995-06-27 | Mycogen Plant Science, Inc. | Octopine T-DNA promoters |
US5567600A (en) | 1983-09-26 | 1996-10-22 | Mycogen Plant Sciences, Inc. | Synthetic insecticidal crystal protein gene |
US5380831A (en) | 1986-04-04 | 1995-01-10 | Mycogen Plant Science, Inc. | Synthetic insecticidal crystal protein gene |
US4771002A (en) | 1984-02-24 | 1988-09-13 | Lubrizol Genetics, Inc. | Transcription in plants and bacteria |
NZ214563A (en) | 1984-12-31 | 1991-05-28 | Univ Georgia | Production of apoaeqorin using recombinant dna |
US5182200A (en) | 1985-04-22 | 1993-01-26 | Lubrizol Genetics, Inc. | T-dna promoters |
US5017692A (en) | 1986-09-04 | 1991-05-21 | Schering Corporation | Truncated human interleukin-a alpha |
US5164316A (en) | 1987-01-13 | 1992-11-17 | The University Of British Columbia | DNA construct for enhancing the efficiency of transcription |
PT89915B (pt) | 1988-03-08 | 1994-10-31 | Ciba Geigy Ag | Processo para a preparacao de sequencias de dna regulaveis quimicamente |
US5445962A (en) | 1988-08-05 | 1995-08-29 | Atallah; Yousef H. | Microorganisms |
US5106739A (en) | 1989-04-18 | 1992-04-21 | Calgene, Inc. | CaMv 355 enhanced mannopine synthase promoter and method for using same |
ATE196318T1 (de) | 1989-10-31 | 2000-09-15 | Monsanto Co | Promotor für transgene pflanzen |
AU648214B2 (en) | 1991-12-31 | 1994-04-14 | Lucky Limited | Recombinant gene coding for human alpha interferon and expression vector thereof, etc. |
HU214698B (hu) | 1992-04-09 | 1998-06-29 | Gyógyszerkutató Intézet Kft. | Eljárás rekombináns deszulfátó-hirudin HV-1 peptidek előállítására |
AU3901993A (en) | 1992-04-13 | 1993-11-18 | Zeneca Limited | Dna constructs and plants incorporating them |
US5563328A (en) | 1992-08-19 | 1996-10-08 | Board Of Regents, University Of Nebraska-Lincoln | Promoters from chlorella virus genes providing for expression of genes in prokaryotic and eukaryotic hosts |
DE69331268T2 (de) | 1993-06-30 | 2002-06-13 | Lucky Ltd | Modifizierte-Granulocyte-Makrophage-Kolonie-stimulierender-Faktor-Gen und seine Expression in Hefe |
US5491084A (en) | 1993-09-10 | 1996-02-13 | The Trustees Of Columbia University In The City Of New York | Uses of green-fluorescent protein |
DE69434624T2 (de) | 1993-11-19 | 2006-12-14 | Biotechnology Research And Development Corp., Peoria | Chimäre regulatorische regionen und gen - kassetten zur genexpression in pflanzen |
US5689052A (en) | 1993-12-22 | 1997-11-18 | Monsanto Company | Synthetic DNA sequences having enhanced expression in monocotyledonous plants and method for preparation thereof |
US5545818A (en) | 1994-03-11 | 1996-08-13 | Calgene Inc. | Expression of Bacillus thuringiensis cry proteins in plant plastids |
US5527883A (en) * | 1994-05-06 | 1996-06-18 | Mycogen Corporation | Delta-endotoxin expression in pseudomonas fluorescens |
US5795737A (en) | 1994-09-19 | 1998-08-18 | The General Hospital Corporation | High level expression of proteins |
US5508264A (en) * | 1994-12-06 | 1996-04-16 | Mycogen Corporation | Pesticidal compositions |
DE4444708A1 (de) | 1994-12-15 | 1996-06-20 | Basf Ag | Verwendung von Herbiziden vom Auxin-Typ zur Behandlung von transgenen Kulturpflanzen |
JP3150862B2 (ja) * | 1995-01-20 | 2001-03-26 | 株式会社荏原製作所 | n−ヘキサン抽出物含有廃水の処理方法 |
DK0815257T3 (da) | 1995-01-31 | 2002-04-22 | Bioimage As | Fremgangsmåde til karakterisering af biologisk aktive stoffer |
GB9504446D0 (en) | 1995-03-06 | 1995-04-26 | Medical Res Council | Improvements in or relating to gene expression |
GB9515941D0 (en) | 1995-08-03 | 1995-10-04 | Zeneca Ltd | DNA constructs |
JP3535177B2 (ja) | 1995-09-22 | 2004-06-07 | バイオイメージ・アクティーゼルスカブ | 緑色蛍光性タンパクであるgfpの新規な変種 |
EE9800257A (et) | 1996-02-22 | 1999-02-15 | Merck & Co., Inc. | Sünteetilised HIV geenid |
AR006928A1 (es) | 1996-05-01 | 1999-09-29 | Pioneer Hi Bred Int | Una molecula de adn aislada que codifica una proteina fluorescente verde como marcador rastreable para la transformacion de plantas, un metodo para laproduccion de plantas transgenicas, un vector de expresion, una planta transgenica y celulas de dichas plantas. |
US6027881A (en) | 1996-05-08 | 2000-02-22 | The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Mutant Aequorea victoria fluorescent proteins having increased cellular fluorescence |
-
1998
- 1998-04-03 EP EP10182774A patent/EP2325308A3/fr not_active Withdrawn
- 1998-04-03 RU RU99123057/13A patent/RU2226215C2/ru active
- 1998-04-03 EP EP98915256A patent/EP1012257A4/fr not_active Ceased
- 1998-04-03 CA CA2285676A patent/CA2285676C/fr not_active Expired - Lifetime
- 1998-04-03 NZ NZ338093A patent/NZ338093A/en not_active IP Right Cessation
- 1998-04-03 JP JP54291598A patent/JP2001519663A/ja active Pending
- 1998-04-03 KR KR19997009092A patent/KR100558838B1/ko not_active IP Right Cessation
- 1998-04-03 AU AU69484/98A patent/AU741974B2/en not_active Expired
- 1998-04-03 WO PCT/US1998/006589 patent/WO1998045424A1/fr active IP Right Grant
-
1999
- 1999-10-01 NO NO19994795A patent/NO324769B1/no not_active IP Right Cessation
-
2008
- 2008-08-20 JP JP2008211702A patent/JP2009039123A/ja active Pending
-
2010
- 2010-02-24 JP JP2010038572A patent/JP2010187673A/ja active Pending
Also Published As
Publication number | Publication date |
---|---|
AU6948498A (en) | 1998-10-30 |
EP1012257A4 (fr) | 2004-08-18 |
CA2285676A1 (fr) | 1998-10-15 |
JP2010187673A (ja) | 2010-09-02 |
WO1998045424A1 (fr) | 1998-10-15 |
NZ338093A (en) | 2001-06-29 |
EP1012257A1 (fr) | 2000-06-28 |
NO994795L (no) | 1999-12-01 |
RU2226215C2 (ru) | 2004-03-27 |
KR20010006014A (ko) | 2001-01-15 |
KR100558838B1 (ko) | 2006-03-10 |
MX9909086A (fr) | 2000-08-31 |
EP2325308A2 (fr) | 2011-05-25 |
JP2009039123A (ja) | 2009-02-26 |
CA2285676C (fr) | 2011-03-08 |
AU741974B2 (en) | 2001-12-13 |
JP2001519663A (ja) | 2001-10-23 |
EP2325308A3 (fr) | 2011-07-27 |
NO994795D0 (no) | 1999-10-01 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US7022896B1 (en) | Methods and materials for making and using transgenic dicamba-degrading organisms | |
US8629323B2 (en) | Methods and materials for making and using transgenic dicamba-degrading organisms | |
NO324769B1 (no) | Isolert DNA molekyl, DNA konstruksjon, renset dicambadegraderende oksygenase, transgen vertscelle og plante, fremgangsmate for dekontaminering av et materiale inneholdende dicamba, for selektering av transformerte planteceller og for selektering, eller screening for, transformerte vertsceller, intakte organismer og deler av organismer. | |
Keller et al. | Transgenic cotton resistant to herbicide bialaphos | |
AU686200B2 (en) | Method of controlling insects | |
EP1556491A2 (fr) | Plantes resistant aux herbicides et polynucleotides et methodes permettant d'obtenir ces plantes | |
EP1379539B1 (fr) | Procedes et materiaux servant a produire et a utiliser des organismes transgeniques degradant dicamba | |
EP0922094A2 (fr) | Gene codant pour un oxalate decarboxylase isole a partir d'aspergillus phoenices | |
AU2002252165A1 (en) | Methods and materials for making and using transgenic dicamba-degrading organisms | |
AU2005203471B2 (en) | Methods and Materials for Making and Using Transgenic Dicamba-Degrading Organisms | |
AU781125B2 (en) | Methods and materials for making and using transgenic dicamba-degrading organisms | |
MXPA99009086A (en) | Methods and materials for making and using transgenic dicamba-degrading organisms |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
MK1K | Patent expired |