EP0998575A1 - Gene chimere codant pour la drosomicine, vecteur le contenant et obtention des plantes transgeniques resistantes aux maladies - Google Patents
Gene chimere codant pour la drosomicine, vecteur le contenant et obtention des plantes transgeniques resistantes aux maladiesInfo
- Publication number
- EP0998575A1 EP0998575A1 EP98936469A EP98936469A EP0998575A1 EP 0998575 A1 EP0998575 A1 EP 0998575A1 EP 98936469 A EP98936469 A EP 98936469A EP 98936469 A EP98936469 A EP 98936469A EP 0998575 A1 EP0998575 A1 EP 0998575A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- peptide
- sequence
- drosomicin
- plants
- chimeric gene
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
- C07K14/43563—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects
- C07K14/43577—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects from flies
- C07K14/43581—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects from flies from Drosophila
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8282—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Definitions
- the subject of the invention is therefore firstly a chimeric gene comprising a nucleic acid fragment coding for a drosomicin as well as heterologous 5 'and 3' regulatory elements capable of functioning in plants and a vector for the transformation of plants containing this chimeric gene. It also comprises a transformed plant cell containing at least one nucleic acid fragment coding for drosomicin and a plant resistant to diseases containing the said cell. Finally, it relates to a process for transforming plants to make them resistant to diseases in which a gene coding for drosomicin is inserted.
- drosomycin of "full length” (total length) as represented by the sequence identifier No. 2 (SEQ ID No. 2).
- the DNA sequence encoding drosomycin comprises the DNA sequence described by bases 21-152 of sequence identifier No. 3 (SEQ ID NO 3), a sequence homologous or complementary to said sequence.
- any corresponding sequence of bacterial origin such as for example the terminator nos of Agrobacterhim tumefaciens, can be used. or of plant origin, such as for example a histone terminator as described in application EP 0 633 317.
- Another series of methods consists in using as a means of transfer into the plant a chimeric gene inserted into a Ti plasmid of Agrobacterhim tumefaciens or Ri of Agrobacterium rhi ⁇ ogenes.
- Oligo 3 5 'GCGCGCGGAT CCTTAGCATC CTTCGCACCA GCACTTCAGA
- This plasmid contains the CaMV 35S promoter which directs the expression of an RNA containing the 5 ′ untranslated sequence of the alfalfa mosaic virus (AMV 5 ′ UTR; Brederode & coll ., 1980), the N-terminal region of the ubql ubql l gene from Arabidopsis thaliana to the ubqit 1 hydrolase cleavage site (ubql 1 N-term; Callis & coll., 1993) which is fused in the same reading frame with the gene for the ⁇ -glucuronidase from E. coli (GUS; Jefferson & coll., 1987). monitoring of the polyadenylation site of the nopaline synthase gene from Agrobaaerium tumefaciens (NOS
- LB have the same meaning as in FIG. 6.
- pRPA-RD-192 Creation of an Agrobacterium tumefaciens vector containing the construction of the gene coding for drosomicin directed towards the cytosol of the cells.
- the DNA fragment containing the drosomicin expression cassette is purified.
- the purified DNA fragment containing the drosomicin expression cassette is linked in pRPA-RD-184, after prior digestion with the same two enzymes.
- An Agrobacterium tumefaciens vector is thus obtained containing the sequence coding for the fusion protein drosomicin-ubiquitin which leads to the expression of drosomicin in the cytosol of plant cells.
- pRPA-RD-193 Creation of an Agrobacterium tumefaciens vector containing the construction of the gene coding for drosomicin directed towards the extracellular matrix.
- Example 2 Tolerance to herbicides in processed tobacco.
- the regeneration of PBD6 tobacco (from SEITA France) from leaf explants is carried out on a Murashige and Skoog (MS) base medium comprising 30 g / l of sucrose as well as 200 ⁇ g / ml of kanamycin.
- the leaf explants are taken from plants cultivated in a greenhouse or in vitro and transformed according to the technique of leaf discs (Horsh & coll., 1985) in three successive stages: the first comprises the induction of shoots on a medium supplemented with 30 g / 1 sucrose containing 0.05 mg / 1 naphthylacetic acid (ANA) and 2 mg / 1 benzylaminopurine (BAP) for 15 days.
- ANA naphthylacetic acid
- BAP benzylaminopurine
- the leaf proteins are first extracted by grinding frozen tissue at -180 ° C. followed by the addition of an extraction buffer (8 M urea, tris HCl pH 6.8 50 raM. 2% SDS. 5% ⁇ -mercaptoethanol. 10% sucrose. 2 mM EDTA and 10 mM dithiothreitol). The total amount of extractable protein is then measured. 100 ⁇ g of extracted proteins are then loaded into SDS-PAGE gel wells (20% acryiamide) for an immunoblot analysis (according to Coligan & coll.).
- Example 4 Resistance to Botrytis cinerea in processed tobacco.
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Insects & Arthropods (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Tropical Medicine & Parasitology (AREA)
- Toxicology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR9709115 | 1997-07-11 | ||
| FR9709115A FR2766206A1 (fr) | 1997-07-11 | 1997-07-11 | Gene chimere codant pour la drosomycine, vecteur le contenant pour la transformation des cellules vegetales et plantes transformees obtenues resistantes aux maladies |
| FR9709663 | 1997-07-24 | ||
| FR9709663A FR2766207B1 (fr) | 1997-07-11 | 1997-07-24 | Gene chimere codant pour la drosomycine, vecteur le contenant pour la transformation des cellules vegetales et plantes transformees obtenues resistantes aux maladies |
| PCT/FR1998/001462 WO1999002717A1 (fr) | 1997-07-11 | 1998-07-08 | Gene chimere codant pour la drosomicine, vecteur le contenant et obtention des plantes transgeniques resistantes aux maladies |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| EP0998575A1 true EP0998575A1 (fr) | 2000-05-10 |
Family
ID=26233684
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP98936469A Withdrawn EP0998575A1 (fr) | 1997-07-11 | 1998-07-08 | Gene chimere codant pour la drosomicine, vecteur le contenant et obtention des plantes transgeniques resistantes aux maladies |
Country Status (13)
| Country | Link |
|---|---|
| US (2) | US6465719B1 (hu) |
| EP (1) | EP0998575A1 (hu) |
| AR (1) | AR016326A1 (hu) |
| AU (1) | AU739137B2 (hu) |
| BR (1) | BR9815507A (hu) |
| CA (1) | CA2296046A1 (hu) |
| CO (1) | CO4810248A1 (hu) |
| FR (1) | FR2766207B1 (hu) |
| HR (1) | HRP980385A2 (hu) |
| HU (1) | HUP0004938A3 (hu) |
| IL (1) | IL133961A0 (hu) |
| PL (1) | PL337994A1 (hu) |
| WO (1) | WO1999002717A1 (hu) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2766207B1 (fr) * | 1997-07-11 | 2000-12-08 | Rhone Poulenc Agrochimie | Gene chimere codant pour la drosomycine, vecteur le contenant pour la transformation des cellules vegetales et plantes transformees obtenues resistantes aux maladies |
| FR2777568B1 (fr) * | 1998-04-15 | 2002-10-31 | Rhone Poulenc Agrochimie | Gene codant pour l'heliomicine, proteine obtenue, vecteur le contenant, organismes transformes obtenus et procede de preparation |
| FR2810993B1 (fr) * | 2000-06-29 | 2002-08-23 | Rhobio | Peptides antimicrobiens de la famille des defensines, polynucleotides codant ces peptides, vecteurs et organismes transformes les contenant |
| JP4944360B2 (ja) * | 2001-05-25 | 2012-05-30 | アンスティテュ ナシオナル ド ラ サント エ ド ラ ルシュルシェ メディカル（アンセルム） | 鉄ホメオスタシスの障害を治療するための医薬品を製造するためのヘプシジンの使用 |
| FR2844142B1 (fr) | 2002-09-11 | 2007-08-17 | Bayer Cropscience Sa | Plantes transformees a biosynthese de prenylquinones amelioree |
| FR2848064B1 (fr) * | 2002-12-06 | 2006-01-13 | Bayer Cropscience Sa | Plantes legumineuses transplastomiques fertiles |
| FR2848570B1 (fr) | 2002-12-12 | 2005-04-01 | Bayer Cropscience Sa | Cassette d'expression codant pour une 5-enol pyruvylshikimate-3-phosphate synthase (epsps) et plantes tolerantes aux herbicides la contenant |
| AR074941A1 (es) | 2009-01-07 | 2011-02-23 | Bayer Cropscience Sa | Plantas transplastomicas exentas de marcador de seleccion |
| US20120311743A1 (en) | 2010-02-02 | 2012-12-06 | Bayer Cropscience Ag | Soybean transformation using hppd inhibitors as selection agents |
| CN110305887A (zh) * | 2018-03-27 | 2019-10-08 | 华南农业大学 | 抗真菌肽Drosomycin、制备方法及其应用 |
| CN109182370B (zh) * | 2018-08-03 | 2022-06-17 | 浙江大学 | 一种植物多基因表达载体、转化子及其应用 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1991019738A1 (de) * | 1990-06-15 | 1991-12-26 | Hoechst Aktiengesellschaft | Antifungisches polypeptid, verfahren zu seiner herstellung |
| FR2673643B1 (fr) * | 1991-03-05 | 1993-05-21 | Rhone Poulenc Agrochimie | Peptide de transit pour l'insertion d'un gene etranger dans un gene vegetal et plantes transformees en utilisant ce peptide. |
| FR2673642B1 (fr) * | 1991-03-05 | 1994-08-12 | Rhone Poulenc Agrochimie | Gene chimere comprenant un promoteur capable de conferer a une plante une tolerance accrue au glyphosate. |
| FR2673703B1 (fr) | 1991-03-06 | 1996-12-06 | Innovations Tech | Appareil a gaz a securite de surchauffe. |
| EP0631629B1 (en) * | 1992-03-20 | 2003-12-03 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Fungus-responsive chimaeric gene |
| KR960705939A (ko) * | 1993-11-19 | 1996-11-08 | 제이. 그랜트 브리븐 | 식물의 유전자 발현을 위한 키메라 조절부위 및 유전자 카세트 |
| US5390926A (en) | 1994-05-31 | 1995-02-21 | Gt Sports Marketing | Practice putting green |
| IL114697A0 (en) * | 1994-07-22 | 1995-11-27 | Demeter Biotech Ltd | Polypeptides comprising ubiquitin-lytic peptide fusion protein products deriving therefrom their preparation and use |
| FR2725992A1 (fr) * | 1994-10-25 | 1996-04-26 | Rhone Poulenc Agrochimie | Peptide antifongique |
| FR2766207B1 (fr) * | 1997-07-11 | 2000-12-08 | Rhone Poulenc Agrochimie | Gene chimere codant pour la drosomycine, vecteur le contenant pour la transformation des cellules vegetales et plantes transformees obtenues resistantes aux maladies |
-
1997
- 1997-07-24 FR FR9709663A patent/FR2766207B1/fr not_active Expired - Fee Related
-
1998
- 1998-07-08 PL PL98337994A patent/PL337994A1/xx unknown
- 1998-07-08 EP EP98936469A patent/EP0998575A1/fr not_active Withdrawn
- 1998-07-08 CA CA002296046A patent/CA2296046A1/fr not_active Abandoned
- 1998-07-08 HU HU0004938A patent/HUP0004938A3/hu unknown
- 1998-07-08 WO PCT/FR1998/001462 patent/WO1999002717A1/fr not_active Application Discontinuation
- 1998-07-08 AU AU85451/98A patent/AU739137B2/en not_active Ceased
- 1998-07-08 IL IL13396198A patent/IL133961A0/xx unknown
- 1998-07-08 BR BR9815507-5A patent/BR9815507A/pt not_active IP Right Cessation
- 1998-07-09 HR HR9709663A patent/HRP980385A2/hr not_active Application Discontinuation
- 1998-07-10 AR ARP980103381A patent/AR016326A1/es not_active Application Discontinuation
- 1998-07-13 CO CO98039656A patent/CO4810248A1/es unknown
-
2000
- 2000-01-11 US US09/480,251 patent/US6465719B1/en not_active Expired - Fee Related
-
2002
- 2002-06-26 US US10/180,247 patent/US20030167519A1/en not_active Abandoned
Non-Patent Citations (1)
| Title |
|---|
| See references of WO9902717A1 * |
Also Published As
| Publication number | Publication date |
|---|---|
| HRP980385A2 (en) | 1999-12-31 |
| CO4810248A1 (es) | 1999-06-30 |
| IL133961A0 (en) | 2001-04-30 |
| AR016326A1 (es) | 2001-07-04 |
| US6465719B1 (en) | 2002-10-15 |
| FR2766207B1 (fr) | 2000-12-08 |
| CA2296046A1 (fr) | 1999-01-21 |
| PL337994A1 (en) | 2000-09-25 |
| HUP0004938A3 (en) | 2002-11-28 |
| FR2766207A1 (fr) | 1999-01-22 |
| HUP0004938A2 (hu) | 2001-04-28 |
| WO1999002717A1 (fr) | 1999-01-21 |
| AU8545198A (en) | 1999-02-08 |
| AU739137B2 (en) | 2001-10-04 |
| BR9815507A (pt) | 2000-11-07 |
| US20030167519A1 (en) | 2003-09-04 |
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