Classifications

• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
  • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
  • C12N15/09—Recombinant DNA-technology
  • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
  • C12N15/1034—Isolating an individual clone by screening libraries
  • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07K—PEPTIDES
  • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
  • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
  • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
  • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07K—PEPTIDES
  • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
  • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
  • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
• • C—CHEMISTRY; METALLURGY
  • C40—COMBINATORIAL TECHNOLOGY
  • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
  • C40B40/00—Libraries per se, e.g. arrays, mixtures
  • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
  • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
  • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
  • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
  • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
  • G01N33/5082—Supracellular entities, e.g. tissue, organisms
  • G01N33/5088—Supracellular entities, e.g. tissue, organisms of vertebrates
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
  • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
  • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
  • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
  • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
  • G01N33/56983—Viruses

Definitions

• This invention relates to the identification of peptide sequences which permit or facilitate the transport of drugs, macromolecules, or particles, such as biodegradable nano- and microparticles, through human or animal tissues.
• this invention relates to the use of phage display libraries in a screening assay in order to determine the identity of peptides sequences which enhance the delivery of the bacteriophage through tissue, such as epithelial cells lining the lumenal side of the gastro-intestinal tract (GIT).
• GIT gastro-intestinal tract
• the epithelial cells lining the lumenal side of the GIT are a major barrier to drug delivery following oral administration.
• folic acid which interacts with the folate receptor
• mannose and cetylmannoside which interact with the mannose receptor
• cobalamin which interacts with Intrinsic Factor.
• leucine- and tyrosine-based peptide sorting motifs or internalization sequences exist, such as YSKV, FPHL, YRGV, YQTI, TEQF, TEVM, TSAF, YTRF, which facilitate uptake or targeting of proteins from the plasma membrane to endosomes.
• Phage display libraries can be screened using specific membrane receptors or binding sites to identify peptides that bind specifically to the receptor or binding site.
• the ability of certain motifs or domains of peptides or proteins to interact with specific membrane receptors, followed by cellular uptake of the proteinrreceptor complex may point towards the potential application of such motifs in facilitating the delivery of drugs.
• the identification of peptides or peptide motifs by their ability to interact with specific receptor sites or carrier sites, such as sites expressed on the apical side of the epithelial sites of the GIT, may not be able to determine, or may not be the most effective way to determine, the identity of peptides capable of enhancing the transport of an active agent, especially a drug-loaded nano- or microparticle, through tissues such as epithelial lining.
• Non-receptor-based assays to discover particular ligands have also been used. For instance, a strategy for identifying peptides that alter cellular function by scanning whole cells with phage display libraries is disclosed in Fong et al., Drug Development
• the invention provides a method of identifying a peptide which permits or facilitates the transport of an active agent through a human or animal tissue.
• a predetermined amount of phage from a random phage library is plated unto or brought into contact with a first side, preferably the apical side, of a tissue sample, either in vitro, in vivo or in situ, or polarized tissue cell culture.
• the phage which is transported to a second side of the tissue opposite the first side, preferably the basolateral side is harvested to select transported phages.
• the transported phages are amplified in a host and this cycle of events is repeated (using the transported phages produced in the most recent cycle) a predetermined number of times, such as from zero to six times, to obtain a selected phage library containing phage which can be transported from the first side to the second side.
• the sequence of at least one random peptide coded by phage in the selected phage library is determined in order to identify a peptide which permits or facilitates the transport of an active agent through a human or animal tissue.
• the transported phage can be viewed as a combination of a transporter peptide (the at least one random peptide coded by the phage) associated with an active agent payload (the phage) in which the transporter peptide facilitates the transport of the active agent through the tissue.
• the random peptides coded by phage in the selected phage library are predictively capable of facilitating transport of other active agents, such as drug encapsulated nano- and/or microparticles, through the particular tissue.
• the tissue sample derives from the duodenum, jejunum, ileum, ascending colon, transverse colon, descending colon, pelvic colon, vascular endothelium cells which line the vascular system, vascular endothelial cells which form the blood brain barrier, alveolar cells, liver, kidney, bone marrow, retinal cells of the eye or neuronal tissue.
• the tissue sample can be either in vitro or in vivo More preferably, the tissue sample comprises epithelial cells lining the lumenal side of the GIT, such as isolated rat colon or small intestine segments or epithelial cells lining the lumenal side of the GIT found in an open or closed loop animal model system.
• Other preferred tissue samples are heart, spleen, pancrease, thymus and brain tissue.
• the polarized tissue cell culture sample is cultured from GIT epithelial cells, alveolar cells, endothelial cells of the blood-brain barrier, or vascular smooth muscle cells. More preferably, the polarized tissue cell culture sample is a polarized Caco-2 cell culture or a polarized T-84 cell culture.
• the active agent is a drug or a nano- or microparticle. More preferably, the active agent is a drug encapsulated or drug loaded nano- or microparticle, such as a biodegradable nano- or microparticle, in which the peptide is physically adsorbed or coated or covalently bonded, such as directly linked or linked via a linking moiety, onto the surface of the nano- or microparticle. Alternatively, the peptide can form the nano- or microparticle itself or can be directly conjugated to the active agent.
• Such conjugations include fusion proteins in which a DNA sequence coding for the peptide is fused in-frame to the gene or cDNA coding for a therapeutic peptide or protein, such that the modified gene codes for a recombinant fusion protein in which the "targeting" peptide is fused to the therapeutic peptide or protein and where the "targeting" peptide increases the absoption of the fusion protein from the GIT.
• Fig. 1 shows the phage yield (% phage transported from the apical to basolateral medium) in the basolateral medium of polarized Caco-2 cells grown on snapwells at cycles 1, 2, 3, and 4 of panning of the X30 phage display library. For each cycle, the basolateral medium was sampled both 1 hour and 24 hours post addition of phage to the apical medium;
• Fig. 2 shows the relative binding to fixed Caco-2 cells of 100 different phage isolates from the X30 phage display library that were obtained from the basolateral medium at completion of cycle 4 (transport from apical to the basolateral medium) panning of the X30 phage display library on Caco-2 snapwells;
• Fig. 3 shows the binding of the negative control phage M13mpl8 and the top ten binders, clones 32, 34, 39, 40, 53, 80, 84, 97, 98 and 100, [each at neat, 1:25 and 1: 100 dilutions] obtained from the X30 library following cycle 4 selection on Caco-2 snapwells to fixed Caco-2 cells.
• the ELISA absorbance reading obtained with fixed Caco-2 cells which were not treated with phage is included;
• Fig.4 shows the binding of the negative control phage M13mpl8 and the top ten binders, clones 32, 34, 39, 40, 53, 80, 84, 97, 98 and 100, [each at neat, 1:25 and 1 : 100 dilutions] obtained from the X30 library following cycle 4 selection on Caco-2 snapwells to fixed Caco-2 cells, but where the background absorbance reading obtained from the fixed Caco-2 cells only, to which no phage was added, has been subtracted; and
• Fig. 5 is a graphical representation of the binding of the phage clones 39, 97 and 100 and the negative control phage M13mpl8, using either neat phage samples or the same phage diluted 1:25 and 1:100, to fixed Caco-2 cells.
• this invention discloses a method of identifying peptides that are capable of facilitating the delivery or transport of an active agent such as a drug across human or animal tissues, including without limitation GIT epithelial layers, alveolar cells, endothelial cells of the blood-brain barrier, vascular smooth muscle cells, vascular endothelial cells, renal epithelial cells, M cells of the Peyers Patch, and hepatocytes.
• an active agent such as a drug across human or animal tissues
• an active agent such as a drug across human or animal tissues
• an active agent such as a drug across human or animal tissues
• an active agent such as a drug across human or animal tissues
• an active agent such as a drug across human or animal tissues
• an active agent such as a drug across human or animal tissues
• an active agent such as a drug across human or animal tissues
• an active agent such as a drug across human or animal tissues
• delivery systems e.g., nanoparticles, microparticles, liposomes, micelles
• fusion proteins can be synthesized, either in vivo or in vitro, whereby the peptide is fused in-frame to a therapeutic peptide or protein active agent such that the peptide enhances the delivery or transport of the therapeutic peptide or protein across the tissue.
• tissue includes, without limitation, the duodenum, jejunum, ileum, ascending colon, transverse colon, descending colon, pelvic colon, the vascular endothelium which line the vascular system, the vascular endothelial cells which form the blood brain barrier, vascular smooth muscle, alveolar, liver, kidney, bone marrow, heart, spleen, pancreas, thymus, brain, spinal, neuronal and retinal eye tissue.
• polarized tissue cell culture refers to cells cultured so as to form polarized cell layers including, without limitation, cell cultures derived from GIT epithelial cells, alveolar cells, endothelial cells of the blood-brain barrier, or vascular smooth muscle cells or any other cell type which upon tissue culturing becomes polarized or adopts morphological characteristics or (topological) structures or appendages specific to that cell type in vivo.
• the term "active agent” includes, without limitation, any drug or antigen or any drug- or antigen-loaded or drug- or antigen-encapsulated nanoparticle, microparticle, liposome, or micellar formulation capable of eliciting a biological response in a human or animal.
• drug- or antigen-loaded or drug- or antigen-encapsulated formulations include those in which the active agent is encapsulated or loaded into nano- or microparticles, such as biodegradable nano- or microparticles, and which have the peptide adsorbed, coated or covalently bonded, such as directly linked or linked via a linking moiety, onto the surface of the nano- or microparticle.
• the peptide can form the nano- or microparticle itself or the peptide can be covalently attached to the polymer or polymers used in the production of the biodegradable nano- or microparticles or drug-loaded or drug- encapsulated nano- or microparticles or the peptide can be directly conjugated to the active agent.
• conjugations to active agents include fusion proteins in which a DNA sequence coding for the peptide is fused in-frame to the gene or cDNA coding for a therapeutic peptide or protein such that the modified gene codes for a recombinant fusion protein.
• drug includes, without limitation, any pharmaceutically active agent.
• Representative drugs include, but are not limited to, peptides or proteins, hormones, analgesics, anti-migraine agents, anti-coagulant agents, anti-emetic agents, cardiovascular agents, anti-hypertensive agents, narcotic antagonists, chelating agents, anti-anginal agents, chemotherapy agents, sedatives, anti-neoplasties, prostaglandins and antidiuretic agents.
• Typical drugs include peptides, proteins or hormones such as insulin, calcitonin, calcitonin gene regulating protein, atrial natriuretic protein, colony stimulating factor, betaseron, erythropoietin
• EPO interferons such as ⁇ , ⁇ or ") interferon, somatropin, somatotropin, somatostatin, insulin-like growth factor (somatomedins), luteinizing hormone releasing hormone (LHRH), tissue plasminogen activator (TPA), growth hormone releasing hormone (GHRH), oxytocin, estradiol, growth hormones, leuprolide acetate, factor Vi ⁇ , interleukins such as interleukin-2, and analogues thereof; analgesics such as fentanyl, sufentanil, butorphanol, buprenorphine, levorphanol, morphine, hydromorphone, hydrocodone, oxymorphone, methadone, lidocaine, bupivacaine, diclofenac, naproxen, paverin, and analogues thereof; anti-migraine agents such as sumatriptan, ergot alkaloids, and analogues thereof; anti-coagulant
• Representative drugs also include antisense oligonucleotides, genes, gene correcting hybrid oligonucleotides, ribozymes, aptameric oligonucleotides, triple-helix forming oligonucleotides, inhibitors of signal transduction pathways, tyrosine kinase inhibitors and DNA modifying agents.
• drug also includes, without limitation, systems for gene delivery and gene therapeutics, including viral systems for gene delivery such as adenovirus, adeono-associated virus, retroviruses, herpes simplex virus, Sindbus virus, liposomes, cationic lipids, dendrimers, imaging agents and enzymes.
• preselected phage library refers to library consisting of a subpopulation of a phage display library. This subpopulation is formed by initially screening against either a target molecule, such as a protein, receptor, enzyme, ion channel, kinase, growth factor or growth factor receptor so as to permit the selection of a subpopulation of phages which specifically bind to the target molecule.
• a target molecule such as a protein, receptor, enzyme, ion channel, kinase, growth factor or growth factor receptor
• the subpopulation can be formed by screening against a target cell or cell type or tissue type, gastro-intestinal track, blood brain barrier or other tissue or tissue barrier so as to permit the selection of a subpopulation of phages which either bind specifically to the target cell or target cell type or target tissue or target tissue barrier, or which binds to and/or is transported across (or between) the target cell, target cell type or target tissue or target tissue barrier either in situ or in vivo.
• This preselected phage library or subpopulation of selected phages can also be rescreened against the target molecule or cell or tissue, permitting the further selection of a subpopulation of phages which bind to the target molecule or taret cell, target tissue or target tissue barrier or which bind to and/or is transported across the target cell, target tissue or target tissue barrier either in situ or in vivo.
• Such rescreening can be repeated from zero to 30 times with each successive "pre-selected phage library," generating additional pre-selected phage libraries.
• human or animal tissue refers to animal tissue explicitly including human tissue.
• NH 2 -terminal amino acid sequence of the absorption proteins pill and pVIH coded by Escherichia coli filementous bacteriophage phage such as fd can be modified by recombinant DNA technology to include a library of random peptide sequences of defined length (Cwirla et al., Proc. Natl. Acad. Sci.USA 87:6378-6382 (1990)).
• a DNA library of modified phage fd sequences, coding for variable pDI or pVTJI proteins can be constructed and propagated in E. coli.
• This invention discloses the use of phage display libraries such as these in a random screening approach or a preselected phage library or subpopulation from a phage display library in a preselected screening approach in order to determine the identity of peptide sequences which enhance the delivery of the bacteriophage from either the apical to basolateral side or the basolateral to apical side of either cultured model systems or in in vitro, in situ or in vivo tissue samples.
• Peptides that enhance the delivery from the apical to basolateral side e.g., gut side to blood side
• plating on the basolateral side might determine peptides useful for raising a mucosal immune response to an antigen administered IV, subcutaneously, transdermally or by the opthalmic route.
• the size of the random peptide sequences coded by the libraries can be of any size.
• the libraries can be designed to code for linear peptides. Alternatively, the libraries can be so designed to contain cysteine residues at two or more fixed positions and thus code for cyclic peptides.
• a preferred bacteriophage fd e.g., from libraries L3.6, L3.15, L8.15 is a filamentous phage having dimensions of approximately 7nm by 500-900nm . On its surface, the phage expresses primarily two different proteins, the gene III protein, of which there are 3-5 copies per phage particle, and the gene VIII protein, of which there are approximately 2,500 copies.
• the genes coding for either gene III or gene VIII have been modified to code for and express random peptide sequences of a particular length, such as 6-mer, 15-mer and 30-mer.
• multiple copies of a DNA insert coding, for example, for a random 15-mer sequence can facilitate the production of random peptide sequences longer than 15-mer.
• Each library represents between 10 8 and 10 9 or more random peptide sequences.
• the phage library can simulate a nanoparticle mixture in which the nanoparticles are coated with different peptides of a specified length.
• DNA insert or partial DNA inserts which may arise due to clevage at internal restriction sites in the DNA library or DNA insert
• DNA insert or partial DNA inserts which may arise due to clevage at internal restriction sites in the DNA library or DNA insert
• Such clones containing multiple DNA inserts, or derivatives thereof, have the capacity to code for longer than expected peptides, due to the presence of the multiple DNA inserts, provided the DNA inserts are in-frame with respect to the gene IH or gene VIII reading frame and/or provided the clones contain internal DNA sequences which are prone to or suseptible to the process of ribosomal frameshifting during translation in vivo, which in turn can restore the reading frame of the DNA insert with respect to the translational reading frame of gene HI or gene VIII, and/or provided the mRNA coded by the DNA insert is in-frame with gene ID or gene VIII and does not contain internal translational stop or translational termination codons, and/or provided any internal translational stop or termination codon(s) can be read as a reading codon(s) by a translational suppressor molecule in vivo, such as the TAG codon which is decoded by the SupE suppressor in E.coli as a GLN codon.
• the peptides coded by triple (or multiple) DNA inserts have the capacity to code for longer and/or more diverse peptides. Such longer peptides have a greater capacity to adopt secondary and tertiary structures as opposed to shorter peptides, such as a 15-mer peptide. This capacity of peptides to adopt defined secondary and/or tertiary structures coded by those phages containing multiple or triple DNA inserts may in-turn account for the selection of these types of phages from random phage display libraries during selection or panning procedures.
• transport mechanisms operate in epithelial cells. Some transport mechanisms are carrier mediated, whereby a carrier or receptor will bind to a ligand and transport the bound ligand into or through the epithelial cell. Other transport systems operate by transcytosis, whereby a carrier or receptor site will bind a ligand, the carrier: ligand complex is internalized by endocytosis and thus delivers a ligand (or drug) into or through the cell.
• This invention allows for the discovery of certain peptide sequences that bind to such active carrier or transcytotic transport systems to facilitate drug delivery.
• the invention discloses the use of a blind or random or preselected screening approach in order to identify peptide sequences that interact with undefined or unknown receptor/carrier sites in tissues, such as epithelial cells, and facilitates the delivery of bacteriophage from the apical to basolateral side of polarized cell cultures or model tissue systems. Because these peptide sequences can facilitate the delivery of a bacteriophage, they are likely to be useful in the transport of drugs and particulate systems, especially the transport of drug loaded or encapsulated nano- and microparticulate systems when coated onto the surface of the same or fusion proteins whereby the peptide is fused to a therapeutic peptide or protein.
• the screening approach in the in vitro context includes contacting a predetermined amount of phage from a random phage library or a preselected phage library with a first side of a human or animal tissue sample or polarized tissue cell culture, harvesting phage which is transported to the opposite side of the tissue sample or culture to select transported phage, amplifying the transported phage in a host and identifying at least one random peptide coded by a transported phage to identify a peptide which permits or facilitates the transport of an active agent through a human or animal tissue.
• the contacting, harvesting and amplifying steps can be repeated a predetermined number of times using the transported phage obtained in the previous cycle. For instance, using polarized tissue cell culture samples such as Caco-2 cells or T-84 cells or tissue extracts such as isolated rat colon segments, phage can be plated to the apical side of the cultured cells or tissue segments. Subsequently, at any desired timepoint but usually from 1 hour to 24 hours, the basolateral medium is harvested aseptically and used to reinfect a host, such as male E. coli coding for the F 1 Factor, to produce progeny.
• a host such as male E. coli coding for the F 1 Factor
• the selected phage from cycle one can be applied to the apical side of the cultured cells or tissue segment and again the phage in the basolateral medium is collected, titered and amplified. Repetition of this cycle allows for enrichment of phage capable of being transported from the apical to basolateral side and thus, the % yield of phage appearing in the basolateral medium increases as the number of cycles increase.
• the DNA sequence coding for the NH 2 -terrninal region of the pill or pVIII protein of the purified, selected, amplified phage(s) is determined to permit deduction of the amino acid sequence of the modified phage(s) which confers the advantage of transport from the apical to basolateral side of the cultured or tissue system.
• the screening approach in the in vivo context includes contacting a predetermined amount of phage from a random phage library or a preselected phage library with a first side of a tissue barrier in vivo, harvesting phage which is transported to the opposite side of the tissue barrier to select transported phage, amplifying the transported phage in a host and identifying at least one random peptide coded by a transported phage to identify a peptide which permits or facilitates the transport of an active agent through a human or animal tissue. If desired, the contacting, harvesting and amplifying steps can be repeated a predetermined number of times using the transported phage obtained in the previous cycle.
• the phage display library can be purified such as by either polyethylene glycol precipitations or sucrose density or CsCl density centrifugations.
• the purified library can then be resuspended, such as in TBS or PBS buffer, and introduced onto one side of a tissue barrier, such as injected into the duodenum, jejunum, ileum, colon or other in vivo animal site using, for instance, a closed loop model or open loop model.
• samples of bodily fluids located across the tissue barrier such as samples of the portal circulation and/or systemic circulation, are withdrawn at predetermined time points, such as 0 to 90 minutes and/or 2 to 6 hours or more.
• the amplified phage present in the culture can be sequenced individually to determine the identity of peptides coded by the phage or, if further enrichment is desired, can be PEG precipitated, resuspended in PBS, and can be either further PEG-precipitated or used directly for administration to another animal closed or open GIT loop model system followed by collection of portal or systemic blood sample and subsequent amplification of the phage transported into such circulation systems.
• administration of the phage display library with, if desired, repeat administration of the amplified phage to the GIT of the animal permits the selection of phage which are transported from the GIT to the portal and/or systemic circulation of the animal.
• the corresponding region of the tissue barrier can be recovered at the end of the procedures given above.
• This recovered tissue can be washed repeatedly in suitable buffers, such as PBS containing protease inhibitors and homogenized, such as in PBS containing protease inhibitors.
• the homogenate can be used to infect a host, such as E. coli, thus permitting amplification of phages which bind tightly to the tissue barrier (e.g., intestinal tissue).
• the recovered tissue can be homogenized in suitable PBS buffers, washed repeatedly and the phage present in the final tissue homogenate can be amplified in E. coli.
• This approach permits amplification (and subsequent identification of the associated peptides) of phages which either bind tightly to the tissue barrier (e.g., intestinal tissue) or which are internalized by the cells of the tissue barrier (e.g., epithelial cells of the intestinal tissue).
• tissue barrier e.g., intestinal tissue
• cells of the tissue barrier e.g., epithelial cells of the intestinal tissue.
• This selection approach of phage which bind to tissues or which are internalized by tissues can be repeated.
• the corresponding peptide sequences coded by the selected phages obtained by the procedures above and identified following DNA sequencing of the appropriate gene HI or gene VIII genes of the phage, are synthesized.
• the binding and transport of the synthetic peptide itself across the model cell culture or isolated tissue system (such as colonic) permits direct assessment of the transport characteristics of each individual peptide.
• fusion of the selected peptide(s) sequences with other peptides or proteins permits direct assessment of the transport of such chimeric proteins or peptides across the model systems.
• Such chimeric proteins or peptides can be synthesized either in vitro or by conventional recombinant technology techniques whereby the cDNA coding for the transporting peptide and the cDNA coding for the drug peptide or protein are ligated together in- frame and are cloned into an expression vector which in turn will permit expression in the desired host, be it prokaryotic cells or eukaryotic cells or transgenic animals or transgenic plants.
• the cDNAs coding for the modified NH 2 -terminal region of the pill proteins can be subcloned into the genes or cDNAs coding for selected protein molecules (e.g., calcitonin, insulin, interferons, interleukines, cytokines, EPO, colony stimulating factors etc.) and these modified genes or cDNAs can be expressed in E. coli or suitable mammalian cells or transgenic animals or transgenic plants.
• the expressed recombinant proteins can be purified and their transcellular, carrier-mediated, transcytotic and or paracellular transport across human or animal tissue can be verified.
• the transporting peptides can be used to coat the surface of nanoparticulate or microparticulate drug delivery vehicles.
• Such coatings can be performed by either direct adsorption of the peptide to the surface of the particulate system or alternatively by covalent coupling of the peptide to the surface of the particulate system, either directly or via a linking moiety or by covalent coupling of the peptide to the polymers used in the production of nanoparticulate or microparticulate drug delivery vehicles, followed by the utilization of such peptide:polymer conjugates in the production of nanoparticulate or microparticulate drug delivery vehicles.
• phage display libraries identified herein as L3.6, L3.15 and L8.15, were obtained from Prof. George P. Smith at the University of Missouri-Columbia. Each library is in the vector fUSE5, which was derived from the parent vector "fd-tet".
• fUSE5 was derived from the parent vector "fd-tet”.
• random 6-mer libraries are expressed by the gene IH of the fd bacteriophage and are displayed on all 5 copies of the resulting protein pill proteins.
• the number of transductant clones amplified is 3.7 x 10" and the size of phage DNA is 9225 bases.
• random 15-mer libraries are expressed by the gene HI of the fd bacteriophage and are displayed on all 5 copies of the resulting protein pin proteins.
• the number of transductant clones amplified is 3.2 x 10"; (secondary amplification) is 12.1 x 10 12 and the size of phage DNA is 9252 bases.
• the vector has two genes VHI in the same genome, one of which is wild type and the other of which displays the foreign residues.
• the random 15-mer libraries are expressed by one of the two genes VH3 of the fd bacteriophage and are displayed on up to approximately 300 copies of the resulting recombinant protein pVIII proteins. This vector is called f88-4, in which the foreign 15-mer is displayed on up to approximately 300 copies of protein pVIII.
• the number of transductant clones amplified is 2.2 x 10 12 and the size of phage DNA is 9273 bases.
• a 30-mer phage display library, X30 was obtained from Dr. Jamie S. Scott of Simon Fraser University.
• the X30 phage display library codes for random peptide sequences 30 residues in size.
• This library was constructed in the f88.4 vector, which carries a tetracycline resistance gene and has two pVIII genes: the wild type gene and a synthetic gene.
• the f88.4 library has variable inserts cloned into the synthetic pVIII gene of the f88.4 vector.
• D38 and DC43 are random phage display libraries in which gene III codes for random peptides of 38 and 43 residues in size, respectively. These libraries are described in McConnell et al, Molecular Diversity 1: 154-176 (1995), US Serial No. 310,192 filed September 21, 1994, US Serial No. 488,161 filed June 7, 1995, and WO 96/09411 , which references are hereby incorporated by reference.
• a large scale preparation of each of the bacteriophage libraries was made in the E. coli host strain K91Kan.
• a single K91Kan colony was innoculated into a sterile 50 ml tube containing 20 ml LB broth (Yeast extract (Gibco) - 1 g; Tryptone (Gibco) - 2 g; NaCl - 1 g; and distilled water - 200 ml) together with kanomycin (final concentration lOO ⁇ g/ml) and grown to mid log phase with 200 rpm agitation at 37° C
• the cells were allowed to incubate with gentle shaking (100 rpm, 37° C) for 5 min to regenerate sheared F pili.
• the cells were pelleted by centrifugation at 2200 rpm for 10 min at room temperature, the supernatant removed and the cells gently resuspended in 20 ml 80 mM NaCl and shaken gently (100 rpm,
• the primary libraries were amplified by inoculating two 1 1 flasks containing
• Phage band - upper band approximately 5 mm, faint, blue, non-flocculent
• PEG - lower band narrow, stringy, flocculent, opaque, white
• the fluid was aspirated off to 2 mm above the phage band and the phage band was withdrawn using a sterile wide aperture transfer pipette and placed in a 26 ml polycarbonate centrifuge tube.
• the tube was filled to the shoulder with TBS, mixed and centrifuged at 50,000 rpm for 4 h at 4°C in the 60Ti rotor (repeated).
• the pellet was dissolved in 10 ml TBS by gentle vortexing and allowed to soften overnight in the cold and revortexed (repeated).
• the pellet was then dissolved in TBS (2 ml per litre of original culture) by vortexing, allowed to soften overnight at 4°C and revortexed.
• the tube was centrifuged briefly to drive solution to the bottom of the tube and transferred to 1.5 ml microtubes.
• Sodium azide (0.02%) can be added and the solution can be heated to 70°C for 20 min to kill residual microorganisms. Following microfuging for 1 min to clear the solution, the supernatant was transferred to sterile microtubes and stored at 4°C.
• 200 ⁇ l of a 1 : 100 dilution was scanned from 240-320 nm to determine the concentration of physical particles and titre 10 ⁇ l of a 10 '8 dilution on 10 ⁇ l of starved K91Kan cells. 200 ⁇ l of the infections was spread on
• the Caco-2 (ATCC designation: CCL 248; derived from a lung metastasis of a colon carcinoma in a 72-year old male) and T-84 cells (ATCC designation: HTB 37; isolated from a primary colonic tumor in a 72 year old Caucasian male) were cultured initially in 25cm 2 flasks, until they reached confluency.
• T84 cells were grown in 1: 1 DMEM:Ham's F12 medium containing 2mM glutamine, 15mM HEPES, 10% fetal calf serum (FCS), 1 % MEM non essential amino acids and SOU ml "1 penicillin and
• Caco-2 cells were grown in DMEM + glutamax-1 containing 10% FCS, 1% MEM non essential amino acids, 50U ml '1 penicillin and 50 ⁇ g ml "1 streptomycin. All cells were incubated at 37°C in 95% O 2 / 5% CO 2 . At confluence the cells were used to seed snapwells.
• the seeding of snapwells was essentially as follows for T-84 cells (a concentration of 1 x 10 6 cells/ l.Oml of medium is required for each 12mm snapwell; a
• the subsequent maintenance and feeding of the cells on the snapwells was as follows: when feeding the wells, the medium was removed from the basolateral side of the snapwell first. The medium was removed from the monolayer with a pipette being careful not to touch the filter and then 1ml of growth medium was place onto the apical side and 2ml of growth medium into the basolateral side. Spillages of medium on the sides of the plate outside the well were checked for and swabbed with a cotton bud moistened with alcohol if necessary. Following seeding on the snap wells, the cells were fed on a daily basis and were cultured on the snapwells for between 21-30 days, during which time the cells spontaneously differentiated and become polarized.
• HBSS Gibco BRL, Cat # 14065-031
• the tubular segment was cut along the mesenteric border to give a flat square piece of tissue.
• the smooth muscle layer was then removed by blunt dissection to leave an approximate 2.5cm 2 patch of epithelium.
• the isolated rat colonic mucosae were mounted in Side-by-side Sweetana- Grass (SG) diffusion chambers.
• the mounted rat colonic mucosae in the S-G chambers were used in the analysis of phage transport from the apical to basolateral side of the colonic tissue.
• the water bath was allowed to equilibrate to 37°C.
• the chambers were filled with HBSS Buffer (see below) and the electrodes are switched on.
• the input-offset control knob was adjusted to zero.
• the system was allowed to equilibrate for approximately 20 minutes, making sure the readings remain at zero throughout.
• the electrodes were switched off and HBSS solution removed. Filters containing sheets of the rat colonic epithelium were mounted on the apparatus and 10 mis of HBSS
• Buffer was added to each side simultaneously.
• Enzyme linked immuno-sorbent assay for fd-derived phage on Caco-2 cells Caco-2 cells (lOO ⁇ l) were grown to confluence in 96 well tissue culture plates
• HRP horse- radish peroxidase
• TMB substrate solution (3,3',5',5-tetramethylbenzidine; Microwell Peroxidase Substrate
• the phage display library is purified such as by either PEG precipitation or by sucrose or CsCl density centrifugation.
• the phage display library is resuspended in PBS (or TBS) buffer and injected into the in vivo animal site, such as duodenum, jejunum, ileum, colon, ascending colon, transverse colon, descending colon, pelvic colon in the closed (or open) animal (rat, rabbit or other species) loop model.
• PBS or TBS
• injected into the in vivo animal site such as duodenum, jejunum, ileum, colon, ascending colon, transverse colon, descending colon, pelvic colon in the closed (or open) animal (rat, rabbit or other species) loop model.
• the corresponding region of the GIT track exposed to or incubated with the phage display library can be recovered at the end of the experiments.
• suitable buffers such as PBS containing protease inhibitors
• the washed tissue is homogenised in PBS containing protease inhibitors and the homogenate is used to infect E.coli, thus permitting amplification of phages which can bind tightly to the intestinal tissue.
• the recovered intestinal tissue can be homogenised in suitable PBS buffers, washed repeatedly and the phage present in the final tissue homogenate can be amplified in E.coli.
• This latter approach also permits amplification of phages which either bind tightly to the intestinal tissue or which are internalized by the epithelial cells of the intestinal tissue
• phage solution was mixed with 900 ⁇ l of growth medium without antibiotic (the complete recommended medium for each cell line but with no antibiotics added) in a microfuge tube.
• the experiment was carried out in duplicate and included a control treatment containing no phage.
• the TER was measured for each snapwell, noting the age of the cells and the passage number. Only intact monolayers of recommended age were used which had expected TER.
• the basolateral medium was replaced in the snapwells with medium without antibiotic and the apical medium was removed.
• the phage solutions and control solutions were added to the apical side of the cells and the snapwell cultures were incubated as normal.
• the medium was removed from the basolateral side and stored in a sterile 2 ml screwcap tube at 4°C. At each time that the basolateral medium is removed, the medium was replaced with fresh medium without antibiotic. When the experiments are finished, the TER was measured and the monolayers were treated with Vircon disinfectant as per normal.
• the phage were titrated by preparing starved cells of E. coli K91 Kan and carrying out serial dilutions of phage in the (growth medium above) in TBS/gelatin.
• K91Kan cell mixture was spread on LB agar plates containing 40 ⁇ g ml '1 tetracycline and lOO ⁇ g/ml kanomycin and grown overnight at 37°C. For a 10 2 dilution (10 ⁇ l into 990 ⁇ l), 200 colonies on a plate represents 1 x 10 7 TU ml "1 .
• the solution is mixed very well by continuously inverting for 2-3 min and stored at 4°C for at least 4 h.
• the precipitated phage is centrifuged for 15 min at 10,000 g (8,500 rpm using Beckman JA17 rotor) in a Beckman J2-MC preparative ultracentrifuge. The supernatant was removed and recentrifuged as before for 5 min.
• the pellet was resuspended in 100 ⁇ l of TBS by leaving for 5 min at room temperature and vortexing (repeat by leaving for 15 min and vortexing again).
• the suspended phage solution was placed in an Oak Ridge tube.
• the supernatant was placed in a clean (preferably sterile) Oak Ridge tube containing 3 ml of PEG/NaCl and mixed by continuous inversion for 2-3 min. Following storage at 4°C for at least 4 h, the tube was centrifuged for 15 min at 13800 g (10,000 rpm using Beckman JA17 rotor) in a Beckman J2-MC preparative ultracentrifuge. The supernatant was removed and recentrifuged as above for 5 min at 10,000 rpm. As much supernatant as possible was removed with a micropipette and the pellet was resuspended in 1 ml of TBS by leaving for 5 min at room temperature and vortexing.
• a clean (preferably sterile) Oak Ridge tube containing 3 ml of PEG/NaCl and mixed by continuous inversion for 2-3 min. Following storage at 4°C for at least 4 h, the tube was centrifuged for 15 min at 13800 g (10,000 rpm using Beckman JA17
• the resuspension was left for 15 min and vortexed again.
• the phage solution was transferred to a 1.5 ml microfuge tube and vortexed again.
• the solution was centrifuged at 13,000 rpm for 30 s in a microfuge and the supernatant was transferred to a fresh 1.5 ml microfuge tube containing 150 ⁇ l PEG/NaCl.
• the tube was mixed by inverting for 2-3 min and stored at 4°C for at least 1 h. Subsequently, the tube was centrifuged at 13,000 rpm for 10 min in a microfuge and the supernatant was removed and recentrifuged for 5 min.
• the pellet was resuspended in 100 ⁇ l of TBS by leaving for 5 min at room temperature and vortexing. The resuspension was left for 15 min and vortexed again.
• This resuspension represents the phage selected in cycle 1.
• One ⁇ l should be withdrawn and used for titration to confirm that approximately 10 9 TU are present.
• the phage solution is now ready for a further round of selection in the cultured T84 and Caco-2 cells, by repeating the steps above using the phage transported into the basolateral medium.
• phage selected from cycle one is now reapplied to the apical side of the Caco-2 or T-84 cells growing on Snapwells.
• the same titre of phage is applied to the apical side of the cells growing on snapwells.
• the titre of phage present in the basolateral medium at each time point is determined and these transported phage are reamplified and recycled back through the cells.
• the % yield of phage which appear in the basolateral medium increases as the number of cycles increase.
• phage have been selected which are preferentially transported from the apical to basolateral side of the cultured cells, due to the random peptide sequences displayed by the bacteriophage gene DI or gene VIII protein products.
• the phage post transfer across rat colon was titrat and amplified as follows
• phage samples titred prior to and after amplification Serial dilutions of phage (2 ⁇ l phage + 18 ⁇ l TBS/gelatin) were performed in microtitre plates and 10 ⁇ l volumes of the required dilutions were transferred to 1.5 ml microtubes. 10 ⁇ l of starved K91Kan cells were added to each microtube, mixed gently and incubated at room temperature for 10 min. 990 ⁇ l of LB + 0.2 ⁇ g ml "1 tetracycline were added and the microtubes were incubated at 37°C for 30 min.
• 200 ⁇ l of the culture were spread on LB (40 ⁇ g ml "1 tetracycline + 100 ⁇ g ml "1 kanamycin) agar plates, incubated at 37°C overnight and the number of colonies were counted.
• the phage was amplified by adding 150 ⁇ l of PEG/NaCl to 1 ml of phage solution (i.e., apical or basolateral HBSS buffer from chambers) in an Oak Ridge tube, mixing by inversion (x 100) and incubating at 4°C for 4 h.
• the tube was centrifuged at 10,000 g for 15 min (JA17 rotor; 8,500 rpm) and the supernatant was decanted and recentrifuged for 5 min. The supernatant was removed and the pellet was resuspended in 100 ⁇ l of TBS (leave at room temperature for 5 min, vortex, leave at room temperature for 15 min and revortex).
• a 5 ⁇ l sample was retained for titration.
• 100 ⁇ l of starved K91Kan cells were added to 95 ⁇ l of phage solution, mixed gently and incubated at room temperature for 30 min.
• 20 ml of pre-warmed LB + 0.2 ⁇ g ml "1 tetracycline were added and the tube was incubated at 37"C and 200 rpm for 30 min.
• 10 ⁇ l of tetracycline (40 mg ml '1 stock) and kanomycin (final concentration of lOO ⁇ g/ml) were added and the tube was incubated overnight at 37°C and 200 rpm.
• the tube was then centrifuged for 15 min at 3440 g (JA17 rotor; 5,000 rpm), the supernatant was added to a new Oak Ridge tube and recentrifuged at 13,800 g (JA17 rotor; 10,000 rpm). The supernatant was transferred to a new Oak Ridge tube containing 3 ml of PEG/NaCl, mixed by inversion ( x 100) and incubated at 4 * 'C for 4 h. The tube was then centrifuged at 13,800 g, the supernatant decanted and recentrifuged at 13,800 g for 5 min. The pellet was resuspended in 100 ⁇ l of TBS
• the phage solution was transferred to a microtube containing 150 ⁇ l of PEG/NaCl, mixed by inversion (x 100) and incubated at 4°C for 1 h.
• the tube was microfuged for 1 min, the supernatant removed and remicrofuged.
• the supernatant was removed and the pellet resuspended in 100 ⁇ l of TBS (leave at room temperature for 5 min, vortex, leave at room temperature for 15 min and revortex). 2 ⁇ l of phage for was removed for titration while the rest was stored at 4°C.
• the phage solution is now ready for a further round of selection in the S-G mounted rat colonic tissue, by repeating the steps above using the phage transported into the basolateral medium.
• phage selected from cycle one is now reapplied to the apical or gut side of the S-G mounted rat colonic tissue.
• the same titre of phage is applied to the gut side of the tissue.
• the titre of phage present in the basolateral medium (blood side) at each time point is determined and these transported phage are reamplified and recycled back through the colonic tissue.
• the % yield of phage which appear in the basolateral medium increases as the number of cycles increase.
• the purified phage display library (random or preselected) is diluted to 500 ⁇ l in PBS buffer and injected into the closed (or open) intestinal loop model (e.g., rat, rabbit or other species).
• the closed (or open) intestinal loop model e.g., rat, rabbit or other species.
• An aliquot of the withdrawn blood can be incubated with E. coli, followed by plating for phage plaques or for transduction units or for colonies where the phage codes for resistance to antibiotics such as tetracycline.
• the remainder of d e withdrawn blood sample (up to 150 ⁇ l) is incubated with 250 ⁇ l of E. coli and 5 ml of LB medium or other suitable growth medium.
• coli cultures are incubated overnight by incubation at 37°C on a shaking platform. Blood samples taken at other time points (such as 15 min, 30 min, 45 min, 60 min up to 6 hours) are processed in a similar manner, permitting amplification of phages present in the portal or systemic circulation in E. coli. at these times.
• the amplified phage is recovered by PEG precipitation and resuspended in PBS buffer or TBS buffer.
• the titer of the amplified phage, before and after PEG precipitation is determined.
• the amplified, PEG precipitated phage is diluted to a known phage titer (generally between 10 8 and 10 10 phage or plaque forming units per ml) and is injected into the GIT of the animal closed (or open) loop model.
• a known phage titer generally between 10 8 and 10 10 phage or plaque forming units per ml
• Blood samples are collected from portal and/or systemic ciruclation at various time points and the phage transported into the blood samples are amplified in E. coli as given above for the first cycle.
• the phage are PEG precipitated, resuspended, titered, diluted and injected into the GIT of the animal closed (or open) loop model.
• This procedure of phage injection followed by collection of portal and/or systemic blood samples and amplification of phage transported into these blood samples can be repeated, for example, up to 10 times, to permit the selection of phages which are preferentially transported from the GIT into the portal and/or systemic circulation.
• Example 1 % Yield of ⁇ in Caco-2 Cells
• a phage mixture comprising libraries L3.6, L3.15 and L8.15 was screened using isolated rat colon according to the procedures given above.
• the phage yield on the basolateral side of the tissue sample is reported as a percentage of the phage applied to the apical side.
• Six successive screening cycles were performed and four 1 h samples of the basolateral buffer were harvested.
• Table 3 reports the % yield of ⁇ in isolated colon segments.
• SEQ ID NO: 2 (a Class of 9 clones - 25% presence)
• SEQ ID NO: 3 (a Class of 5 clones - 13.9% presence)
• SEQ ID NO: 4 (a Class of 3 - 8.3% presence) were determined from this 36 clone sample from cycle 6. All of these Classes consist of clones with triple DNA inserts. Individual isolates are given by SEQ. ID. NO: 5 to SEQ ID NO: 9 (triple DNA inserts) and SEQ ID NO: 10
• oligonucleotides ELN93 and ELN94 correspond to a partial coding region in those phage clones for SEQ ID NO: 2 and SEQ ID NO: 3, respectively.
• the incidence of reactivity per screening cycle is summarized in Table 4 below.
• the incidence of reactivity per screening cycle in Caco-2 and T-84 cells is compared to reactivity in colon tissue in Table 5 (ELN93) and Table 6 (ELN94).
• probe reactivity is expressed as a percentage of the total number of colonies screened per phage population. Some reactivity was detected in Caco-2 selected clones using ELN93.
• FIG. 1 summarizes phage yield (% phage transported from the apical to basolateral medium) at cycles 1, 2, 3 and 4 in the basolateral medium of polarized Caco-2 cells grown on snapwells.
• the basolateral medium was sampled both 1 hour and 24 hour post addition of phage to the apical medium.
• the basolateral medium was removed after one hour and replaced with fresh basolateral medium. Subsequently, the basolateral medium was removed 24 hours post addition of the initial phage library.
• the phage present was quantitated by titering a sample of each basolateral medium in Escherichia coli K91Kan strain. The remaining basolateral medium from the one hour and twenty four hour sampling time point was combined, the phage present were PEG-precipitated, the precipitated phage was resuspended in lOO ⁇ l of TBS and was used to infect Escherichia coli K91Kan, thus permitting amplification of the phage present in the basolateral medium as outlined previously. Following amplification, the amplified phage was titered, PEG- precipitated, resuspended in TBS and titered.
• the phage suspension was now ready for the next round of further selection in the cultured Caco-2 cells, by repeating the steps above using the phage transported into the basolateral medium, as oudined previously. Upon going from cycle 1 to 4, there was a 19.2 fold enrichment of phage which are transported from the apical to basolateral medium of the Caco-2 cells grown on snapwells.
• Figure 2 summarizes the relative binding of 100 different phage isolates to fixed Caco-2 cells.
• the 100 individual phages from the X30 library were obtained from the cycle 4 selection (transport from apical to the basolateral medium) of cultured Caco-2 cells grown on snapwells.
• Caco-2 cells were grown to confluence in 96 well tissue culture plates as described above, followed by fixing in 10% formaldehyde as described above.
• the ELISA analysis was performed using the HRP -anti-M13 conjugate.
• the binding of each phage isolate is arranged or presented so that the "weakest" to "strongest” binding phage are presented from left to right (and not the numerical number of the phage isolate).
• the binding of the negative control phage (M13mpl8) and the absorbance readings obtained with untreated fixed Caco-2 cells is shown on the extreme right of Fig. 3, respectively.
• Figure 3 summarizes the binding of the top ten binders, clones 32, 34, 39, 40, 53, 80, 84, 97, 98, and 100, to fixed Caco-2 cells, along with the binding of the negative control phage M13mpl8 to the fixed Caco-2 cells, with phage binding monitored by ELISA analysis as described above.
• the binding studies were performed in duplicate, using neat phage (-10 10 pfu/ml) or diluted phage samples (diluted 1:25 and 1: 100 in each case).
• the absorbance readings obtained using the fixed Caco-2 cells in which no phage was added is shown on the right hand side of Figure 3.
• Figure 4 is essentially the same as Figure 3, except that the background absorbance readings obtained using the fixed Caco-2 cells only, to which no phage was added, has been subtracted from the absorbance readings obtained using fixed Caco-2 cells which were incubated with the indicated phage clone samples and the negative control phage M13mpl8.
• the precise titers of neat phage used for each clone are given in Table 7.
• Figure 5 is a graphical representation of the binding of the phage clones 39, 97 and 100, and the negative control phage M13mpl8, to fixed Caco-2 cells using either neat phage samples (at - 10'° pfu/ml) or the same phage diluted 1:25 and 1: 100.
• the phage binding experiments and subsequent ELISA analysis was performed as previously outlined.
• This data shows that the phage clones 39, 97 and 100 bind in a dose response manner, with reduction in the ELISA absorbance readings obtained following dilution of the phage either 1:25 or 1: 100.
• the negative control phage M13mpl8 does not bind in a dose response manner, with linear absorbance readings obtained using either neat, 1 : 25 or 1 : 100 diluted phage.
• the top ten binders, clones 32, 34, 39, 40, 53, 80, 84, 97, 98 and 100 were sequenced using procedures outlined above. Eight of these sequences were identical to the sequence of clone 97 giving DNA sequence SEQ. NO. ID: 11 and peptide sequence SEQ. NO. ID: 12. The two remaining clones (53 and 100) produced individual isolates DNA SEQ. NOS. ID: 13 and 15 with the corresponding peptide sequences SEQ. NOS. ID: 14 and 16, respectively.
• One skilled in the art could determine without undue experimentation which fragments of these peptides permit or facilitate the transport of an active agnet through a human or animal tissue. On the basis of the results of Example 4, it is expected that these fragments consist of at least 6 amino acid residues.
• phage from random phage display libraries as well as control phage were injected into the lumen of the rat gastro-intestinal tract (in situ rat closed loop model). Blood was collected over time from either the systemic circulation or portal circulation and the number of phage which were transported to the circulation was determined by titering blood samples in E. coli.
• the phage display libraries used in this study were D38 and DC43 in which gene HI codes for random 38-mer and 43-mer peptides, respectively.
• As a negative control the identical phage M13mpl8, in which gene III does not code for a "random" peptide sequence, was used.
• Both the library phages D38 and DC43 were prepared from E.coli, mixed together, dialyzed against PBS, precipitated using PEG/NaCl and were resuspended in PBS buffer.
• the M13mpl8 control was processed in a similar manner. The titer of each phage sample was determined and the phage samples were diluted in PBS to approximately the same titers prior to injection into the rat closed loop model.
• phage solution was injected into the closed loop and blood was sampled from the portal vein catheter at various times. As the portal sampling is delicate, sampling times were restricted to 15, 30, 45 and 60 minutes, where possible.
• the volume of phage injected into each animal was as follows:
• mice Rl, R2 and R3 received the control phage M13mpl8 and animals R4, R5, R6 and R7 received the test phage D38 / DC43 mix.
• animals R8, R9 and RIO received the control phage M13mpl8 and animals Rl 1, R12,
• R13 and R14 received the test phage D38 / DC43 mix.
• Animal R15* received the combined phage samples from animals R4-R7 (see Table 8) which were sampled from the systemic circulation on day one, followed by amplifiction in E. coli, PEG precipitation and resuspension in PBS. On subsequent analysis, the titer of this phage was found to 100 times greater than the other phage samples used for animals R8 -
• phage was removed from the "amplified" supernatants obtained from test animals #R4-R7 (samples from each time point were used), combined and was PEG-precipitated for two hours.
• the precipitated phage was resuspended in PBS buffer and was injected into closed loop model of animal #R15, followed by portal sampling.
• the number of phage transported from the closed loop model into the systemic circulation is presented in Table 8 .
• the number of phage transported from the closed loop model into me portal circulation is presented in Table 9. These numbers are corrected for phage input difference and for volume input differences.
• more phage are present in the portal samples than in me systemic samples, indicative of either hepatic or RES clearance and/or phage instability in the systemic circulation.
• the uptake of phage from the GIT into die portal circulation is quite rapid, with substantial number of phages detected within 15 minutes.
• the results from the portal sampling experiments would also indicate that the kinetics of uptake of phage from the D38 / DC43 libraries is quicker than that of the control phage.
• % of the phage transported into the titered blood sample within the limited time frame (30, 45 and 15 mins, respectively) is estimated as 0.13%, 1.1% and 0.013%, respectively.
• Animal R15* received the combined phage samples from animals R4-R7 (see Table 8) which were sampled from the systemic circulation on day one, followed by PEG precipitation and resuspension in PBS. On subsequent analysis, the titer of this phage was found to be 100 times greater than the other phage samples used for animals R8 - R14. Thus, the date presented for animal R15* in Table 9 is adjusted down.
• Phage from the D38/DC43 libraries which appeared in the systemic circulation of different animals (R4-R7) were pooled, amplified in E.coli, precipitated, and re-applied to the lumen of the GIT, followed by collection in the portal circulation and titering in E.coli. These selected phage were also transported from die lumen of the GIT into the portal circulation.
• This in situ loop model may represent an attractive screening model in which to identify peptide sequences which facilitate transport of phage and particles from the GIT into the circulation.
• phage libraries Four preselected phage libraries, GI-D, GI-S, GI-H and GI-P, are constructed by pooling phage previously selected by screening random phage display libraries
• these preselected phage libraries together with the negative control phage M13mpl8 are injected into the rat closed loop model (6 animals per preselected phage library), blood is collected over time from the portal circulation via the portal vein and, at the termination of the experiment, a systemic blood sample is collected from the tail vein and the intestinal tissue region from the closed loop is collected.
• phages selected in vitro to each receptor or binding site located in the GIT were amplified in E. coli, PEG-precipitated, resuspended in TBS and the titer of each phage sample was determined by plaquing in E.coli as described above. Subsequently, an equal number of each phage (8 x 10 8 phage) for each receptor site was pooled into a preselected phage library together with the negative control phage M13mpl8 and each preselected phage library was administered to 6 Wistar rats per library (rats 1-6; GI-D, rats 7-12; GI-S, rats 13-18; GI-P, and rats 19-24; GI-H).
• the E.coli was removed by centrifugation and the amplified phage supernatant samples were eidier titered directly or were PEG- precipitated, resuspended in TBS and titered. Following titration of the amplified phage, samples containing phage from each set of animals were combined, adjusting the titer of each sample to the same titer, and were plated for plaques on LB agar plates (22cm 2 square plates). Either 12,000 or 24,000 phage were plated for plaques.
• the intestinal tissue portion used in each closed loop was excised.
• the tissue was cut into small segments, followed by 3 washings in sterile PBS containing protease inhibitors, and homogenized in an Ultra thorex homogeniser (Int-D samples).
• the tissue in PBS supplemented with protease inhibitors was homogenized in an Ultra Thorex homogeniser, washed 3 times in PBS containing protease inhibitors and resuspended in PBS containing protease inhibitors (Int-G samples).
• serial dilutions (neat and 10 , 10 "4 , 10 * dilutions) of the tissue homogenate was titered in E.coli.
• tissue homogenate was added to lOO ⁇ l of E.coli K91Kan, incubated at 37°C for 10 min, followed by addition of 5ml of LB medium and incubation overnight at 37°C in a rotating microbial incubator.
• the phage amplified from the portal blood, systemic blood and intestinal tissue was plated for plaques.
• the plaques were transferred to Hybond-N Nylon filters, followed by denaturation (1.5M NaCl, 0.5M NaOH), neutralization (0.5M
• oligonucleotides (22-mers), complimentary to regions coding for the receptor or binding sites used to create the preselected phage library, were synthesized.
• the oligonucleotides (5pmol) were 5'end labelled with 32 P-ATP and T4 polynucleotide kinase and approximately 2.5pmol of labelled oligonucleotide was used in hybridization studies.
• Hybridization's were performed at 40-45°C overnight in buffer containing 6X SSC, 5X Denhardt's solution, 0.1% SDS, 20 ⁇ g/ml yeast tRNA and die radiolabeled syntiietic oligonucleotide, followed by washings (20-30 min at 40-45°C) in the following buffers: (i) 2X SSC / 0.1% SDS, (ii) IX SSC / 0.1% SDS, (iii) 0. IX SSC / 0.1 % SDS. The filters were air-dried and exposed for autoradiography for 15hours, 24hours or 72hours.
• Table 10 summarises the results from the hybridization studies outlined above. Apart from the synthetic oligonucleotide to HAX9, all oligonucleotides were initially confirmed to be radiolabeled, as determined by hybridisation to the corresponding phage target (eg., phage S 15 hybridised to the oligonucleotide S15). In addition, under the experimental conditions used the oligonucleotides essentially did not hybridise to the negative control phage template M13mpl8.
• oligonucleotides were synthesised to die phage M13mpl8 - (1) a positive oligonucleotide which hybridises to a conserved sequence in botii M13mpl8 and each of die GIT receptor or GIT binding site selected phages [designated M13 (positive)] in Table 10 and (2) a negative oligonucleotide which only hybridises to a sequence unique to die multiple cloning site of phage M13mpl8 and which does not hybridise to any of the GIT receptor or GIT binding site selected phages.
• phages S21, S22, SNI-28, SNI-45, SNIAX-2, SNIAX-6 and SNIAX-8 are not transported from die GIT into the portal circulation.
• phages SNI-10 and to a lesser extent phages S 15 and S22 were found in the intestine samples or fractions, whereas the otiier phages were not.
• phages In the case of the GI-D pool of phages, there is a rank order by which phages are transported from the GIT closed loop model into the portal circulation, with phages DCXl 1 and D. ⁇ B 10 preferably transported, followed by phages DCX8, DAB30, DAB3 and DAB7. A number of phages from this pool are not transported into the portal circulation, including phages DAB 18, DAB 24, DAX15, DAX24, DAX27, DCX26, DCX36, DCX39, DCX42, DCX45. There is a very low level of transport of phage DAX23 from the GIT into the portal circulation.
• Some phages are not found in the intestinal samples, including phages DAB 18, DAB24, DAX 15, DAX24, DCX26, and DCX36.
• phages can be further selected from pre-selected libraries, permitting the identification of phages which are transported from the GIT closed loop into the portal circulation or phages which bind to or are internalised by intestinal tissue.
• phages In the case of the GI-H pool of phages, there is a rank order by which phages are transported from the GIT closed loop model into the portal or systemic circulation, with phages PAX2 (which was used at a 4 x concentration relative to the other phages in this pool) followed by phage HAX42 found in die portal and systemic circulation and phage H40 found in the systemic circulation only. None of the phages in this pool were found in the intestine samples or fractions. Phage M13mpl8 was not found in the intestine fractions or systemic circulation, with very low incidence ( ⁇ 0.001%) in the portal circulation. These results show that phages can be further selected from pre-selected libraries, permitting the identification of phages which are transported from the GIT closed loop into the portal and/or systemic circulation or phages which bind to or are internalised by intestinal tissue.
• phages PAX2 and H40 were also included in this pool.
• a number of phages from this pool were found in the portal circulation, including phages P31, PAX46, PAX9, H40, PAX17, PAX40, PAX2,
• a number of phages were not found in the portal blood including the negative control phage M13mpl8, PAX15, PAX16, PAX18, PAX35, PAX38, PAX43, PAX45, P90, 5PAX5 and 5PAX7.
• the only phage found in the systemic circulation were phages 5PAX5 and P31.
• phages can be further selected from pre-selected libraries, permitting the identification of phages which are transported from the GIT closed loop into the portal and/or systemic circulation or phages which bind to or are internalised by intestinal tissue.
• ACGCCCACAA ACACGCAGAA GACGCCGGAG AAAAAGTGCA AAGCTTTGCC ATTTTGCTGC 120
• MOLECULE TYPE other nucleic acid
• DESCRIPTION: /desc "individual isolate”
• CTCAGCCTTA TTAGGTGCCT TGGATCCTCT GGTCGCCTAT GCCTGCAGCA AGCAGTAGTA 60
• MOLECULE TYPE DNA (genomic)
• FEATURE FEATURE
• MOLECULE TYPE DNA (genomic)
• MOLECULE TYPE DNA (genomic)
• MOLECULE TYPE other nucleic acid

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• 1996
  • 1996-11-11 PT PT96938439T patent/PT859959E/pt unknown
  • 1996-11-11 NZ NZ322174A patent/NZ322174A/xx unknown
  • 1996-11-11 DK DK96938439T patent/DK0859959T3/da active
  • 1996-11-11 ES ES96938439T patent/ES2203722T3/es not_active Expired - Lifetime
  • 1996-11-11 EP EP96938440A patent/EP0876615A1/de not_active Withdrawn
  • 1996-11-11 EP EP96938439A patent/EP0859959B1/de not_active Expired - Lifetime
  • 1996-11-11 WO PCT/IE1996/000073 patent/WO1997017614A1/en not_active Application Discontinuation
  • 1996-11-11 AU AU75852/96A patent/AU705816B2/en not_active Ceased
  • 1996-11-11 JP JP51802597A patent/JP4135978B2/ja not_active Expired - Fee Related
  • 1996-11-11 CA CA002234685A patent/CA2234685A1/en not_active Abandoned
  • 1996-11-11 NZ NZ322175A patent/NZ322175A/xx not_active IP Right Cessation
  • 1996-11-11 WO PCT/IE1996/000072 patent/WO1997017613A1/en active IP Right Grant
  • 1996-11-11 AT AT96938439T patent/ATE246808T1/de active
  • 1996-11-11 DE DE69629385T patent/DE69629385T2/de not_active Expired - Lifetime
  • 1996-11-11 JP JP51802697A patent/JP3830525B2/ja not_active Expired - Fee Related
  • 1996-11-11 EP EP02021532A patent/EP1281718A3/de not_active Withdrawn
  • 1996-11-11 AU AU75853/96A patent/AU705688B2/en not_active Ceased
  • 1996-11-11 CA CA002235226A patent/CA2235226A1/en not_active Abandoned
• 2005
  • 2005-11-30 JP JP2005345975A patent/JP4126055B2/ja not_active Expired - Fee Related

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