EP0642587A4 - System zur messung der proteolytischen aktivität von periodontopathogenen bakterien. - Google Patents
System zur messung der proteolytischen aktivität von periodontopathogenen bakterien.Info
- Publication number
- EP0642587A4 EP0642587A4 EP92913226A EP92913226A EP0642587A4 EP 0642587 A4 EP0642587 A4 EP 0642587A4 EP 92913226 A EP92913226 A EP 92913226A EP 92913226 A EP92913226 A EP 92913226A EP 0642587 A4 EP0642587 A4 EP 0642587A4
- Authority
- EP
- European Patent Office
- Prior art keywords
- chromophore
- test substance
- specimen
- microorganisms
- activity
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/06—Quantitative determination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2337/00—N-linked chromogens for determinations of peptidases and proteinases
- C12Q2337/10—Anilides
- C12Q2337/12—Para-Nitroanilides p-NA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2337/00—N-linked chromogens for determinations of peptidases and proteinases
- C12Q2337/30—Naphthyl amides, e.g. beta-NA, 2-NA, 4-methoxy-beta-naphthylamine, i.e. 4MNA
Definitions
- This invention relates generally to systems for determining the presence of oral diseases in mammals, and more particularly, to a colori ⁇ metric test which is useful in determining periodontal disease activity in a human being, or in an animal.
- periodontal disease has traditionally been defined as an inflammation of the gums, which means that host tissue is responding to bacteria and/or the products of bacteria, periodontal disease has not been treated like a bacterial infection in the medical sense.
- periodontal disease has not been treated in the art with antimicrobial drugs because the growth of plaque on the teeth appears to be inevitable and since it is external to the body, would not seem to be treatable by systemically administered drugs.
- periodontal disease was specific to one, or several, particularly damaging bacteria. In fact, about 200 species of microbes have been isolated from various plaque samples. Thus, mechanical treatment which requires instrumenting of the tooth to remove accumulated bacterial deposits non- specifically was deemed to be the appropriate means of treating periodontal disease.
- Spirochetes and black pigmented bacteroides are particularly prominent when pockets bleed upon probing and when there is clinical evidence of disease progression. Thus, the possibility of drug treatment directed towards these anaerobic organisms is raised. In fact, beneficial results have been observed with the use of metronidazole, an antimicrobial effective against anaerobes.
- metronidazole is available underthe trademark FLAGYL from G. D. Searle & Co., Chicago, IL 60680 as well as in generic form from Zenith Laboratories, Inc., Ramsey, NJ.
- the specimen is additionally assayed for a nonspecific enzymatic marker for indicating whether the specimen contains a predetermined minimum number of microorganisms sufficient to yield a reliable test result.
- a nonspecific enzymatic marker for indicating whether the specimen contains a predetermined minimum number of microorganisms sufficient to yield a reliable test result.
- Analysis of the results of extensive testing has revealed that a population of approximately between 1 and 10 million bacteria in the specimen is sufficient to yield reliable results with the proteolytic enzyme test.
- the test procedure may yield a negative result solely because there were too few bacteria in the specimen.
- the first chromogenic test substance is specific to trypsin-like enzymatic activity, and is selected from the group consisting of BANA, BAPNA, and 2-arginine-7-amino-4-trifluoromethyl coumarin.
- the second chromogenic test substance is specific to the enzyme phosphatase, and is selected from the group consisting of p-nitrophenol phosphate, o-nitro- phenol phosphate, and o-carboxyl phosphate.
- the peptide substrate is benzoyl-DL-arginine-p-nitroanilide (BAPNA) which forms color as it hydrolyses, thereby obviating the need for an additional color developer to demonstrate the enzymatic activity of the specimen.
- BAPNA benzoyl-DL-arginine-p-nitroanilide
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Toxicology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US38597789A | 1989-07-26 | 1989-07-26 | |
US07/634,924 US5116735A (en) | 1989-07-26 | 1990-12-24 | Diagnosing periodontal disease by measuring proteolytic activity of periodontopathogenic bacteria |
PCT/US1992/004333 WO1993024651A1 (en) | 1989-07-26 | 1992-05-22 | System for measuring proteolytic activity of periodontopathogenic bacteria |
BR9207133A BR9207133A (pt) | 1992-05-22 | 1992-05-22 | Sistema para medir atividade proteolitica de bactéria periodontopatogênica |
Publications (2)
Publication Number | Publication Date |
---|---|
EP0642587A1 EP0642587A1 (de) | 1995-03-15 |
EP0642587A4 true EP0642587A4 (de) | 1997-03-19 |
Family
ID=27160062
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP92913226A Withdrawn EP0642587A4 (de) | 1989-07-26 | 1992-05-22 | System zur messung der proteolytischen aktivität von periodontopathogenen bakterien. |
Country Status (2)
Country | Link |
---|---|
EP (1) | EP0642587A4 (de) |
WO (1) | WO1993024651A1 (de) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IT1401376B1 (it) | 2010-08-09 | 2013-07-18 | Skf Ab | Unita' di guida e tenuta, in particolare per uno stelo di un ammortizzatore monotubo e complesso anulare di tenuta per la stessa |
WO2020218052A1 (ja) * | 2019-04-25 | 2020-10-29 | アドテック株式会社 | 歯周病原因菌の検出方法 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1991014000A1 (en) * | 1990-03-15 | 1991-09-19 | The University Of Michigan | System for diagnosing periodontal disease |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0321160B2 (de) * | 1979-05-02 | 1991-03-22 | Nat Res Dev | |
JPS5817598B2 (ja) * | 1979-10-31 | 1983-04-08 | 味の素株式会社 | 微生物の迅速検出方法 |
JPS5696700A (en) * | 1979-12-31 | 1981-08-04 | Sankin Kogyo Kk | Composition for diagnosing tooth decay activity |
JPS58225029A (ja) * | 1982-06-21 | 1983-12-27 | Showa Yakuhin Kako Kk | う蝕簡易判別用具 |
US4568535A (en) * | 1982-08-06 | 1986-02-04 | Loesche Walter J | Composition for periodontal administration |
CH683616A5 (de) * | 1984-06-18 | 1994-04-15 | Univ Michigan | Diagnostisches Testverfahren zum Nachweis von oralen pathogenen Bakteriengemischen. |
-
1992
- 1992-05-22 WO PCT/US1992/004333 patent/WO1993024651A1/en not_active Application Discontinuation
- 1992-05-22 EP EP92913226A patent/EP0642587A4/de not_active Withdrawn
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1991014000A1 (en) * | 1990-03-15 | 1991-09-19 | The University Of Michigan | System for diagnosing periodontal disease |
Non-Patent Citations (3)
Title |
---|
B.E. LAUGHON ET AL.: "API ZYM system for identification of Bacterioides spp., Capnocytophaga spp., and spirochetes of oral origin.", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 15, no. 1, 1982, CHICAGO IL USA, pages 97 - 102, XP000614320 * |
See also references of WO9324651A1 * |
W.J. LOESCHE ET AL.: "Multi-center clinical evaluation of a chairside method for detecting certain periodontal disease.", JOURNAL OF PERIONDOTOLOGY, vol. 61, no. 3, 1990, ANN ARBOR MI USA, pages 189 - 196, XP002023003 * |
Also Published As
Publication number | Publication date |
---|---|
EP0642587A1 (de) | 1995-03-15 |
WO1993024651A1 (en) | 1993-12-09 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US5223403A (en) | Device for diagnosing periodontal disease | |
JP2763635B2 (ja) | 生体液中のジアミンの検出 | |
Loesche | DNA probe and enzyme analysis in periodontal diagnostics | |
Bretz et al. | Characteristics of trypsin-like activity in subgingival plaque samples | |
EP0255341B1 (de) | Reagens zur Prüfung von periodentalen Erkrankungen | |
US4976951A (en) | Dental caries diagnostic and localization technique | |
US5116735A (en) | Diagnosing periodontal disease by measuring proteolytic activity of periodontopathogenic bacteria | |
AU674829B2 (en) | Diagnosing periodontal disease by measuring proteolytic activity of periodontopathogenic bacteria | |
US3691018A (en) | Diagnostic method for periodontal disease | |
Beighton et al. | Trypsin-like, chymotrypsin-like and glycylprolyl dipeptidase activities in gingival crevicular fluid from human periodontal sites with gingivitis | |
EP0880602A1 (de) | Schneller test für mikrobielle protease | |
WO1993024651A1 (en) | System for measuring proteolytic activity of periodontopathogenic bacteria | |
JPH01201161A (ja) | 体液のアッセイ方法及びキット | |
US4994376A (en) | Detection of bacteroides gingivalis | |
AU626523B2 (en) | Diagnostic methods, product and kits for detecting peridontal diseases | |
JP3413199B2 (ja) | 歯周病病原性細菌のタンパク質加水分解活性の測定系 | |
JP2662227B2 (ja) | 歯周病原性菌検査薬 | |
CA2136416A1 (en) | System for measuring proteolytic activity of periodontopathogenic bacteria | |
JP2004507252A (ja) | サンプル中の標的微生物検出のための組成物および方法 | |
Malagi | Chairside diagnostic test kits in periodontics–a review | |
Beighton et al. | Protease activity in gingival crevicular fluid from discrete periodontal sites in humans with periodontitis or gingivitis | |
JP2662228B2 (ja) | 歯周病原性菌検査薬 | |
EP0380932B1 (de) | Festephasentest zum Nachweis der periodentalen Krankheit in einem subgingivalem Fleck | |
JPH0611240B2 (ja) | 歯周疾患検査薬 | |
Rosaiah et al. | The Detection Of Bana Hydrolysis Activity In Chronic Periodontitis. |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
|
17P | Request for examination filed |
Effective date: 19941222 |
|
AK | Designated contracting states |
Kind code of ref document: A1 Designated state(s): AT BE CH DE DK ES FR GB GR IT LI LU MC NL SE |
|
A4 | Supplementary search report drawn up and despatched | ||
AK | Designated contracting states |
Kind code of ref document: A4 Designated state(s): AT BE CH DE DK ES FR GB GR IT LI LU MC NL SE |
|
17Q | First examination report despatched |
Effective date: 19970929 |
|
GRAG | Despatch of communication of intention to grant |
Free format text: ORIGINAL CODE: EPIDOS AGRA |
|
GRAH | Despatch of communication of intention to grant a patent |
Free format text: ORIGINAL CODE: EPIDOS IGRA |
|
STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN |
|
18D | Application deemed to be withdrawn |
Effective date: 20000406 |