EP0642587A4 - Systeme de mesure de l'activite proteolytique des bacteries parodontopathogenes. - Google Patents

Systeme de mesure de l'activite proteolytique des bacteries parodontopathogenes.

Info

Publication number
EP0642587A4
EP0642587A4 EP92913226A EP92913226A EP0642587A4 EP 0642587 A4 EP0642587 A4 EP 0642587A4 EP 92913226 A EP92913226 A EP 92913226A EP 92913226 A EP92913226 A EP 92913226A EP 0642587 A4 EP0642587 A4 EP 0642587A4
Authority
EP
European Patent Office
Prior art keywords
chromophore
test substance
specimen
microorganisms
activity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP92913226A
Other languages
German (de)
English (en)
Other versions
EP0642587A1 (fr
Inventor
Walter J Loesche
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Michigan
Original Assignee
University of Michigan
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US07/634,924 external-priority patent/US5116735A/en
Application filed by University of Michigan filed Critical University of Michigan
Priority claimed from BR9207133A external-priority patent/BR9207133A/pt
Publication of EP0642587A1 publication Critical patent/EP0642587A1/fr
Publication of EP0642587A4 publication Critical patent/EP0642587A4/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/06—Quantitative determination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2337/00—N-linked chromogens for determinations of peptidases and proteinases
    • C12Q2337/10—Anilides
    • C12Q2337/12—Para-Nitroanilides p-NA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2337/00—N-linked chromogens for determinations of peptidases and proteinases
    • C12Q2337/30—Naphthyl amides, e.g. beta-NA, 2-NA, 4-methoxy-beta-naphthylamine, i.e. 4MNA

Definitions

  • This invention relates generally to systems for determining the presence of oral diseases in mammals, and more particularly, to a colori ⁇ metric test which is useful in determining periodontal disease activity in a human being, or in an animal.
  • periodontal disease has traditionally been defined as an inflammation of the gums, which means that host tissue is responding to bacteria and/or the products of bacteria, periodontal disease has not been treated like a bacterial infection in the medical sense.
  • periodontal disease has not been treated in the art with antimicrobial drugs because the growth of plaque on the teeth appears to be inevitable and since it is external to the body, would not seem to be treatable by systemically administered drugs.
  • periodontal disease was specific to one, or several, particularly damaging bacteria. In fact, about 200 species of microbes have been isolated from various plaque samples. Thus, mechanical treatment which requires instrumenting of the tooth to remove accumulated bacterial deposits non- specifically was deemed to be the appropriate means of treating periodontal disease.
  • Spirochetes and black pigmented bacteroides are particularly prominent when pockets bleed upon probing and when there is clinical evidence of disease progression. Thus, the possibility of drug treatment directed towards these anaerobic organisms is raised. In fact, beneficial results have been observed with the use of metronidazole, an antimicrobial effective against anaerobes.
  • metronidazole is available underthe trademark FLAGYL from G. D. Searle & Co., Chicago, IL 60680 as well as in generic form from Zenith Laboratories, Inc., Ramsey, NJ.
  • the specimen is additionally assayed for a nonspecific enzymatic marker for indicating whether the specimen contains a predetermined minimum number of microorganisms sufficient to yield a reliable test result.
  • a nonspecific enzymatic marker for indicating whether the specimen contains a predetermined minimum number of microorganisms sufficient to yield a reliable test result.
  • Analysis of the results of extensive testing has revealed that a population of approximately between 1 and 10 million bacteria in the specimen is sufficient to yield reliable results with the proteolytic enzyme test.
  • the test procedure may yield a negative result solely because there were too few bacteria in the specimen.
  • the first chromogenic test substance is specific to trypsin-like enzymatic activity, and is selected from the group consisting of BANA, BAPNA, and 2-arginine-7-amino-4-trifluoromethyl coumarin.
  • the second chromogenic test substance is specific to the enzyme phosphatase, and is selected from the group consisting of p-nitrophenol phosphate, o-nitro- phenol phosphate, and o-carboxyl phosphate.
  • the peptide substrate is benzoyl-DL-arginine-p-nitroanilide (BAPNA) which forms color as it hydrolyses, thereby obviating the need for an additional color developer to demonstrate the enzymatic activity of the specimen.
  • BAPNA benzoyl-DL-arginine-p-nitroanilide
EP92913226A 1989-07-26 1992-05-22 Systeme de mesure de l'activite proteolytique des bacteries parodontopathogenes. Withdrawn EP0642587A4 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US38597789A 1989-07-26 1989-07-26
US07/634,924 US5116735A (en) 1989-07-26 1990-12-24 Diagnosing periodontal disease by measuring proteolytic activity of periodontopathogenic bacteria
PCT/US1992/004333 WO1993024651A1 (fr) 1989-07-26 1992-05-22 Systeme de mesure de l'activite proteolytique des bacteries parodontopathogenes
BR9207133A BR9207133A (pt) 1992-05-22 1992-05-22 Sistema para medir atividade proteolitica de bactéria periodontopatogênica

Publications (2)

Publication Number Publication Date
EP0642587A1 EP0642587A1 (fr) 1995-03-15
EP0642587A4 true EP0642587A4 (fr) 1997-03-19

Family

ID=27160062

Family Applications (1)

Application Number Title Priority Date Filing Date
EP92913226A Withdrawn EP0642587A4 (fr) 1989-07-26 1992-05-22 Systeme de mesure de l'activite proteolytique des bacteries parodontopathogenes.

Country Status (2)

Country Link
EP (1) EP0642587A4 (fr)
WO (1) WO1993024651A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT1401376B1 (it) 2010-08-09 2013-07-18 Skf Ab Unita' di guida e tenuta, in particolare per uno stelo di un ammortizzatore monotubo e complesso anulare di tenuta per la stessa

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991014000A1 (fr) * 1990-03-15 1991-09-19 The University Of Michigan Systeme de diagnostic de parodontolyse

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0321160B2 (fr) * 1979-05-02 1991-03-22 Nat Res Dev
JPS5817598B2 (ja) * 1979-10-31 1983-04-08 味の素株式会社 微生物の迅速検出方法
JPS5696700A (en) * 1979-12-31 1981-08-04 Sankin Kogyo Kk Composition for diagnosing tooth decay activity
JPS58225029A (ja) * 1982-06-21 1983-12-27 Showa Yakuhin Kako Kk う蝕簡易判別用具
US4568535A (en) * 1982-08-06 1986-02-04 Loesche Walter J Composition for periodontal administration
CH683616A5 (de) * 1984-06-18 1994-04-15 Univ Michigan Diagnostisches Testverfahren zum Nachweis von oralen pathogenen Bakteriengemischen.

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991014000A1 (fr) * 1990-03-15 1991-09-19 The University Of Michigan Systeme de diagnostic de parodontolyse

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
B.E. LAUGHON ET AL.: "API ZYM system for identification of Bacterioides spp., Capnocytophaga spp., and spirochetes of oral origin.", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 15, no. 1, 1982, CHICAGO IL USA, pages 97 - 102, XP000614320 *
See also references of WO9324651A1 *
W.J. LOESCHE ET AL.: "Multi-center clinical evaluation of a chairside method for detecting certain periodontal disease.", JOURNAL OF PERIONDOTOLOGY, vol. 61, no. 3, 1990, ANN ARBOR MI USA, pages 189 - 196, XP002023003 *

Also Published As

Publication number Publication date
WO1993024651A1 (fr) 1993-12-09
EP0642587A1 (fr) 1995-03-15

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