EP0479283A2 - Verfahren zum Absaugen und Trocknen der Kavitäten von beschichteten Mikrotiterplatten - Google Patents

Verfahren zum Absaugen und Trocknen der Kavitäten von beschichteten Mikrotiterplatten Download PDF

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Publication number
EP0479283A2
EP0479283A2 EP91116856A EP91116856A EP0479283A2 EP 0479283 A2 EP0479283 A2 EP 0479283A2 EP 91116856 A EP91116856 A EP 91116856A EP 91116856 A EP91116856 A EP 91116856A EP 0479283 A2 EP0479283 A2 EP 0479283A2
Authority
EP
European Patent Office
Prior art keywords
cannula
drying
aspirating
cups
cavity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP91116856A
Other languages
English (en)
French (fr)
Other versions
EP0479283A3 (en
EP0479283B1 (de
Inventor
Heinz-Dieter Klein
Bernhard Dr. Giesendorf
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Siemens Healthcare Diagnostics GmbH Germany
Original Assignee
Hoechst AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoechst AG filed Critical Hoechst AG
Publication of EP0479283A2 publication Critical patent/EP0479283A2/de
Publication of EP0479283A3 publication Critical patent/EP0479283A3/de
Application granted granted Critical
Publication of EP0479283B1 publication Critical patent/EP0479283B1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L13/00—Cleaning or rinsing apparatus
    • B01L13/02—Cleaning or rinsing apparatus for receptacle or instruments
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0678—Facilitating or initiating evaporation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates

Definitions

  • reagents which are not bound or are bound nonspecifically are removed with buffer solutions containing detergent in some cases.
  • the washing liquids are drawn off via suction heads and the titer plates are dried in a second step using a turbo dryer.
  • Whirled compressed air flows into the cavity via a nozzle.
  • the swirled droplets are aspirated with a cannula.
  • a disadvantage of this method in addition to the discontinuous mode of operation, is that the environment is exposed to aerosols and that microdroplets remain on the wall of the cavity, which cannot be removed from the wall even by increasing the air volume and speed.
  • This residual moisture must be mixed with desiccants e.g. Silica gel in the sealed microtiter plate can be reduced to a relative humidity of ⁇ 10% in order to ensure the shelf life of the coating.
  • the invention seeks to remedy this.
  • the invention solves the problem by a method, which is characterized in that a cannula with an inner diameter of 0.5 to 3 mm is lowered into the cavity at a lowering speed of 0.5 to 20 mm / s, the cavity being moved over the Cannula with a suction power of 50 to 2500 1 / h is emptied.
  • the advantages of the invention can be seen essentially in the fact that the washing liquid can be removed in one step and no residual liquid in the cavities remain so that the relative humidity of ⁇ 10% is reached after the microplate has been sealed in after a short time.
  • the figure shows the decrease in the relative humidity in the packaging of a microtiter plate as a function of time, namely Curve 1 for an untreated, uncoated microtiter plate as an empty test; Curve 2 for a coated microtiter plate, which was processed according to the invention, ie a cannula with an inner diameter of 1.2 mm was lowered at a lowering speed of 4.2 mm / s into the cavity filled with washing liquid, the cavity with the cannula a suction power of 400 l / h was emptied and Curve 3 shows a coated microtiter plate which was processed in accordance with the prior art, ie after washing the plate was dried in a second step with a turbo dryer from Sandy Spring Inc. for 5 seconds.

Abstract

Bei dem Verfahren zum Absaugen und Trocknen der Kavitäten von beschichteten Mikrotiterplatten wird eine Kanüle mit einem Innendurchmesser von 0,5 bis 3 mm mit einer Absenkgeschwindigkeit von 0,5 bis 20 mm/s in die Kavität abgesenkt, und die Kavität über die Kanüle mit einer Saugleistung von 50 bis 2500 l/h entleert. Dabei wird die Kanüle 0,05 bis 3 mm oberhalb des Kavitätenbodens angehalten.
Die Figur zeigt die Abnahme der relativen Luftfeuchtigkeit in der Verpackung einer Mikrotiterplatte in Abhängigkeit von der Zeit, un zwar
Kurve 1 für einer unbehandelte, unbeschichtete Mikrotiterplatte als Leerversuch;
Kurve 2 für eine beschichtete Mikrotiterplatte, die gemäβ Erfindung bearbeitet wurde;
Kurve 3 eine beschichtete Mikrotiterplatte, die gemäβ Stand der Technik bearbeitet wurde.

Description

  • Bei beschichteten Mikrotiterplatten werden nicht oder unspezifisch gebundene Reagenzien gewaschen mit zum Teil detergenzhaltigen Pufferlösungen entfernt. Die Waschflüssigkeiten werden über Absaugköpfe abgezogen und die Titerplatten in einem zweiten Schritt mit einem Turbotrockner getrocknet. Dabei strömt über eine Düse verwirbelte Pressluft in die Kavität. Die hochgewirbelten Tröpfchen werden mit einer Kanüle abgesaugt. Nachteilig bei diesem Verfahren ist neben der diskontinuierlichen Arbeitsweise eine Aerosolbelastung der Umgebung und das Zurückbleiben von Mikrotropfen an der Wand der Kavität, die selbst durch vergrößern der Luftmenge und - Geschwindigkeit nicht mehr von der Wand entfernt werden können. Diese Restfeuchte muß mit Trockenmitteln z.B. Silicagel in der eingesiegelten Mikrotiterplatte auf eine relative Luftfeuchtigkeit von < 10 % abgesenkt werden, um die Lagerfähigkeit der Beschichtung zu gewährleisten.
  • Hier will die Erfindung Abhilfe schaffen. Die Erfindung löst die Aufgabe durch ein Verfahren, das dadurch gekennzeichnet ist, daß eine Kanüle mit einem Innendurchmesser von 0,5 bis 3 mm mit einer Absenkgeschwindigkeit von 0,5 bis 20 mm/s in die Kavität abgesenkt wird, wobei die Kavität über die Kanüle mit einer Saugleistung von 50 bis 2500 1/h entleert wird.
  • Es kann zweckmäßig sein, die Kanüle 0,05 bis 3 mm über dem Kavitätenboden anzuhalten.
  • Die Vorteile der Erfindung sind im wesentlichen darin zu sehen, daß die Waschflüssigkeit in einem Schritt entfernt werden kann und keine Restflüssigkeit in den Kavitäten zurückbleiben, so daß die relative Luftfeuchtigkeit von < 10 % nach dem Einsiegeln der Mikrotiterplatte nach kurzer Zeit erreicht wird.
  • Die Figur zeigt die Abnahme der relativen Luftfeuchtigkeit in der Verpackung einer Mikrotiterplatte in Abhängigkeit von der Zeit, und zwar
    Kurve 1 für eine unbehandelte, unbeschichtete Mikrotiterplatte als Leerversuch;
    Kurve 2 für eine beschichtete Mikrotiterplatte, die gemäß Erfindung bearbeitet wurde, d.h. eine Kanüle mit einem Innendurchmesser von 1,2 mm wurde mit einer Absenkgeschwindigkeit von 4,2 mm/s in die mit Waschflüssigkeit gefüllte Kavität abgesenkt, wobei die Kavität über die Kanüle mit einer Saugleistung von 400 l/h entleert wurde und
    Kurve 3 eine beschichtete Mikrotiterplatte, die gemäß Stand der Technik bearbeitet wurde, d.h. nach dem Waschen wurde die Platte in einem 2. Schritt mit einem Turbotrockner der Firma Sandy Spring Inc. 5 Sekunden getrocknet.

Claims (2)

  1. Verfahren zum Absaugen und Trocknen der Kavitäten von beschichteten Mikrotiterplatten, dadurch gekennzeichnet, daß eine Kanüle mit einem Innendurchmesser von 0,5 bis 3 mm mit einer Absenkgeschwindigkeit von 0,5 bis 20 mm/s in die Kavität abgesenkt wird, wobei die Kavität über die Kanüle mit einer Saugleistung von 50 bis 2500 l/h entleert wird.
  2. Verfahren nach Anspruch 1, dadurch gekennzeichnet, daß die Kanüle 0,05 bis 3 mm oberhalb des Kavitätenbodens angehalten wird.
EP91116856A 1990-10-05 1991-10-02 Verfahren zum Absaugen und Trocknen der Kavitäten von beschichteten Mikrotiterplatten Expired - Lifetime EP0479283B1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE4031500A DE4031500A1 (de) 1990-10-05 1990-10-05 Verfahren zum absaugen und trocknen der kavitaeten von beschichteten mikrotiterplatten
DE4031500 1990-10-05

Publications (3)

Publication Number Publication Date
EP0479283A2 true EP0479283A2 (de) 1992-04-08
EP0479283A3 EP0479283A3 (en) 1992-11-25
EP0479283B1 EP0479283B1 (de) 1994-09-07

Family

ID=6415621

Family Applications (1)

Application Number Title Priority Date Filing Date
EP91116856A Expired - Lifetime EP0479283B1 (de) 1990-10-05 1991-10-02 Verfahren zum Absaugen und Trocknen der Kavitäten von beschichteten Mikrotiterplatten

Country Status (10)

Country Link
US (1) US5236513A (de)
EP (1) EP0479283B1 (de)
JP (1) JP3053473B2 (de)
AT (1) ATE110984T1 (de)
AU (1) AU645351B2 (de)
CA (1) CA2052807A1 (de)
DE (2) DE4031500A1 (de)
DK (1) DK0479283T3 (de)
ES (1) ES2063427T3 (de)
ZA (1) ZA917950B (de)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10144225C1 (de) * 2001-09-08 2003-02-06 Eppendorf Ag Verfahren zum Trocknen von Filtern, die in Kavitäten einer Mikrotiterfilterplatte angeordnet sind
DE102005016755B3 (de) 2005-04-11 2006-11-30 Eppendorf Ag Vorrichtung, System mit einer solchen Vorrichtung und Verfahren zum Trocknen von Kavitäten in Mikrotiterfilterplatten und von darin angeordneten Filtern

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4496657A (en) * 1982-10-14 1985-01-29 Scripps Clinic And Research Foundation Microplate washer
AT391097B (de) * 1987-11-17 1990-08-10 Prolic Sa Reinigungsvorrichtung fuer kuevetten

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3330160A1 (de) * 1983-08-20 1985-03-07 Boehringer Ingelheim KG, 6507 Ingelheim Monoklonaler antikoerper mit hoher affinitaet zum digoxin

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4496657A (en) * 1982-10-14 1985-01-29 Scripps Clinic And Research Foundation Microplate washer
AT391097B (de) * 1987-11-17 1990-08-10 Prolic Sa Reinigungsvorrichtung fuer kuevetten

Also Published As

Publication number Publication date
DK0479283T3 (da) 1995-02-13
JPH04248437A (ja) 1992-09-03
EP0479283A3 (en) 1992-11-25
AU645351B2 (en) 1994-01-13
ZA917950B (en) 1992-06-24
JP3053473B2 (ja) 2000-06-19
DE4031500A1 (de) 1992-04-09
US5236513A (en) 1993-08-17
DE59102826D1 (de) 1994-10-13
ES2063427T3 (es) 1995-01-01
ATE110984T1 (de) 1994-09-15
CA2052807A1 (en) 1992-04-06
AU8563991A (en) 1992-04-09
EP0479283B1 (de) 1994-09-07

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