EP0352609B1 - Verwendung von Adenosinderivaten zur Herstellung von Arzneimitteln mit immunstimulierender Wirkung - Google Patents

Verwendung von Adenosinderivaten zur Herstellung von Arzneimitteln mit immunstimulierender Wirkung Download PDF

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Publication number
EP0352609B1
EP0352609B1 EP89113194A EP89113194A EP0352609B1 EP 0352609 B1 EP0352609 B1 EP 0352609B1 EP 89113194 A EP89113194 A EP 89113194A EP 89113194 A EP89113194 A EP 89113194A EP 0352609 B1 EP0352609 B1 EP 0352609B1
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European Patent Office
Prior art keywords
adenosine derivatives
compositions
formula
fact
acyl radical
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Expired - Lifetime
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EP89113194A
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English (en)
French (fr)
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EP0352609A1 (de
Inventor
Federico Gennari
Luigi Bassi
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Bioresearch SRL
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Bioresearch SRL
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Priority to AT89113194T priority Critical patent/ATE84720T1/de
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals

Definitions

  • This invention relates to the use of adenosine derivatives for the preparation of pharmaceutical composition for the immunotheraphy of congenital and acquired deficiency and of tumours.
  • the immunostimulants form a heterogeneous category of medicaments used for potentiating inadequate or depressed immune responses.
  • Immunostimulants are classified according to their origin, namely endogenous such as thymic hormones, monokines, lymphokines, interferons, or natural exogenous (generally obtained from microorganisms) such as BCG, Corynebacterium Parvum, Muramyl dipeptide, Bestatin and Lentinan, or also synthetis exogenous such as Levamisole, Isoprenosine and Tuftsin.
  • endogenous such as thymic hormones, monokines, lymphokines, interferons, or natural exogenous (generally obtained from microorganisms) such as BCG, Corynebacterium Parvum, Muramyl dipeptide, Bestatin and Lentinan, or also synthetis exogenous such as Levamisole, Isoprenosine and Tuftsin.
  • immunostimulants The main fields of application of immunostimulants are the immunotherapy of congenital and acquired immunodeficiencies and tumour immunotherapy.
  • tumour therapy since the immunogenicity of certain experimental tumours and the ability to induce their remission by acting on the immune functions of the host were demonstrated several years ago, numerous attempts have been directed towards establishing an immunotherapy for human tumours. These results have been discouraging, but in reality these were premature attempts the failure of which was caused by empiricism, poor knowledge of tumour biology and the interactions between the tumour and host, and the indiscriminate and improper use of immunostimulants.
  • R are: methyl, ethyl, propyl, isopropyl, butyl, isobutyl, sec-butyl, pentyl, hexyl, heptyl, octyl, decyl, hexadecyl, octadecyl, benzyl.
  • R1 are: hydrogen, acetyl, propionyl, butyryl, succinyl, glutaryl, benzoyl, tosyl.
  • R2 are: hydrogen, acetyl, propionyl, butyryl, succinyl, glutaryl, benzoyl, tosyl.
  • the preferred acid addition salts of the compounds of formula (I) are: chloride, sulphate, hydrogensulphate, phosphate, formate, acetate, citrate, tartrate, lactate, methanesulphonate, p-toluenesulphonate.
  • the immunostimulant activity was initially demonstrated for certain of the terms of general formula (I) by using four experimental tests, the results of which are given in Tables A, B, C and D.
  • the treatment was carried out during the inductive stage of the response.
  • Tuftsin The effect of a known immunostimulant, Tuftsin, is shown at the foot of Table A.
  • MTA is again seen to be the most active compound of the series under examination. with maximum efficiency for a dose of 10 mg/kg.
  • MDP Muramyl dipeptide
  • immunostimulant capacity was also evaluated on a model of macrophage cytotoxic activity against target tumour induced by Corynebacterium Parvum.
  • Table C shows that all the tested compounds are able to potentiate the inducing effect of Corynebacterium Parvum.
  • the obtained microphage activation is equal to or very often greater than that obtained by an optimum dose of Corynebacterium.
  • Table D shows that when administered alone to the animal, the product does not modify the spontaneous NK activity. However, when associated with Poly I:C it potentiates its effect by reducing the polynucleotide dose required to obtain maximum activation.
  • the tested products influence specific immunity by amplifying both humoral and cell-mediated immune responses. They do not appear to directly influence natural immunity, but when associated with conventional macrophage and NK function inducers they considerably potentiate their effect, so greatly reducing the activator dose required for optimum response.
  • the compounds of the present invention are practically free of acute toxicity when administered orally. They are also practically free of toxicity at therapeutic doses for any method of administration.
  • adenosine derivatives of formula (I) can be administered orally, parenterally, intravenously or rectally in any therapeutically useful form, either alone or diluted with suitable pharmacologically acceptable excipients. They can also be used in products for external topical application, and in other forms normally used in the pharmaceutical field.
  • the active principle content of the pharmaceutical compositions of the present invention corresponds to a therapeutic administration of between 5 and 20 mg/kg of body weight.

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Epidemiology (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Saccharide Compounds (AREA)

Claims (11)

  1. Verfahren zur Herstellung von pharmazeutischen Zubereitungen für die Immuntherapie von angeborener und erworbener Immundefizienz und von Tumoren, welche als Wirkstoff ein Adenosinderivat der allgemeinen Formel (I)
    Figure imgb0024
     worin:
    R = ein geradekettiger oder verzweigter C₁-C₁₈-Alkylrest oder ein Phenylalkylenrest, dessen Alkylenkette 1-6 C-Atome aufweist;
    R₁ = H, ein C₁-C₆-aliphatischer Acylrest oder ein C₇-aromatischer Acylrest;
    R₂ = H, ein C₁-C₆-aliphatischer Acylrest oder ein C₇-aromatischer Acylrest oder die zwei Reste R₂ bilden gemeinsam eine Isopropylidenkette;
    in Kombination mit passenden pharmazeutisch geeigneten Exzipienten enthalten, welches das Vermischen dieses Adenosinderivats mit den Exzipienten umfaßt.
  2. Verfahren gemäß Anspruch 1, dadurch gekennzeichnet, daß R eine unter Methyl, Ethyl, Propyl, Isopropyl, Butyl, Isobutyl, sec-Butyl, Pentyl, Hexyl, Heptyl, Octyl, Decyl, Hexadecyl, Octadecyl, Benzyl ausgewählte Gruppe, R₁ eine unter Wasserstoff, Acetyl, Propionyl, Butyryl, Succinyl, Glutaryl, Benzoyl, Tosyl ausgewählte Gruppe und R₂ eine unter Wasserstoff, Acetyl, Propionyl, Butyryl, Succinyl, Glutaryl, Benzoyl, Tosyl ausgewählte Gruppe bedeuten.
  3. Verfahren gemäß Anspruch 1, dadurch gekennzeichnet, daß R₁ = R₂ = H, R = CH₃.
  4. Verfahren gemäß Anspruch 1, dadurch gekennzeichnet, daß es die Verwendung eines pharmakologisch geeigneten Säure-Additionssalzes umfaßt, wenn R₁ = H.
  5. Verfahren gemäß Anspruch 4, dadurch gekennzeichnet, daß das Säure-Additionssalz unter Chlorid, Sulfat, Hydrogensulfat, Phosphat, Formiat, Acetat, Citrat, Tartrat, Lactat, Methansulfonat und p-Toluolsulfonat ausgewählt ist.
  6. Verfahren gemäß Anspruch 1, dadurch gekennzeichnet, daß die Zubereitungen geeignet sind für die Verabreichung auf oralem, parenteralem, intravenösem, rektalem oder äußerlich topischem Weg.
  7. Verfahren gemäß Anspruch 1, dadurch gekennzeichnet, daß die Zubereitungen in Form von Kapseln ausgebildet sind, die 50 bis 100 mg dees Derivats der Formel (I) enthalten, worin R₁ = R₂ = H, R = CH₃.
  8. Verfahren gemäß Anspruch 1, dadurch gekennzeichnet, daß die Zubereïtungen in Form von Tabletten ausgebildet sind, die 50 bis 100 mg des Derivats der Formel (I) enthalten. worin R₁ = R₂ = H, R = CH₃.
  9. Verfahren gemäß Anspruch 1, dadurch gekennzeichnet, daß die Zubereitungen in Form von Suppositorien ausgebildet sind, die 50 bis 100 mg des Derivats der Formel (I) enthalten, worin R₁ = R₂ = H, R = CH₃.
  10. Verfahren gemäß Anspruch 1, dadurch gekennzeichnet, daß die Zubereitungen in Form von Fläschchen mit Injektionsflüssigkeit ausgebildet sind, die 25 bis 50 mg des Derivats der Formel (I) enthalten, worin R₁ = R₂ = H, R = CH₃.
  11. Verfahren gemäß Anspruch 1, dadurch gekennzeichnet, daß die Zubereitungen in Form von Fläschchen mit der Zusammensetzung für orale Verabreichung ausgebildet sind, die 50 bis 100 mg des Derivats der Formel (I) enthalten, worin R₁ = R₂ = H, R = CH₃.
EP89113194A 1988-07-29 1989-07-19 Verwendung von Adenosinderivaten zur Herstellung von Arzneimitteln mit immunstimulierender Wirkung Expired - Lifetime EP0352609B1 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AT89113194T ATE84720T1 (de) 1988-07-29 1989-07-19 Verwendung von adenosinderivaten zur herstellung von arzneimitteln mit immunstimulierender wirkung.

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IT2157088 1988-07-29
IT8821570A IT1227049B (it) 1988-07-29 1988-07-29 Impiego di derivati adenosinici per la preparazione di composizioni farmaceutiche aventi attivita' immunostimolante.

Publications (2)

Publication Number Publication Date
EP0352609A1 EP0352609A1 (de) 1990-01-31
EP0352609B1 true EP0352609B1 (de) 1993-01-20

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Country Status (7)

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EP (1) EP0352609B1 (de)
JP (1) JP2611003B2 (de)
AT (1) ATE84720T1 (de)
DE (1) DE68904491T2 (de)
ES (1) ES2053880T3 (de)
GR (1) GR3006831T3 (de)
IT (1) IT1227049B (de)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5780595A (en) * 1989-06-16 1998-07-14 Cor Therapeutics, Inc. Platelet aggregation inhibitors
IT1243859B (it) * 1990-10-23 1994-06-28 Bioresearch Spa Composizioni farmaceutiche comprendenti associazioni fra sali di s-adenosil-l-metionina e acido 5-metil (o 5-formil)-tetraidrofolico per la terapia di complicanze neurologiche negli ammalati di aids.
DK1891961T3 (da) 2005-03-17 2009-12-21 Proyecto Biomedicina Cima Sl Anvendelse af 5'-methylthioadenosin (MTA) i forebyggelsen og/eller behandling af autoimmune sygdomme og/eller transplantatafstödning
ES2259552B1 (es) * 2005-03-17 2007-06-16 Proyecto De Biomedicina Cima, S.L. Empleo de 5'-metiltioadenosina (mta) en la prevencion y/o tratamiento de enfermedades autoinmunes y/o rechazo de transplantes.
ES2329327B1 (es) 2008-03-19 2010-09-17 Proyecto De Biomedicina Cima, S.L. Combinaciones sinergicas de 5'-metiltioadenosina.

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2313937A1 (fr) * 1975-06-09 1977-01-07 Anvar Medicament a base de 5' thioethers de l'adenosine
FR2354100A2 (fr) * 1976-06-08 1978-01-06 Anvar Medicament a base de 5' thioethers de l'adenosine
IT1193529B (it) * 1980-04-22 1988-07-08 Bioresearch Srl Derivati adenosinici ad attivita' antinfiammatoria ed analgesica e composizioni terapeutiche che li contengono come principio attivo

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Publication number Publication date
JP2611003B2 (ja) 1997-05-21
DE68904491D1 (de) 1993-03-04
DE68904491T2 (de) 1993-05-13
JPH0278621A (ja) 1990-03-19
GR3006831T3 (de) 1993-06-30
ES2053880T3 (es) 1994-08-01
ATE84720T1 (de) 1993-02-15
IT1227049B (it) 1991-03-14
EP0352609A1 (de) 1990-01-31
IT8821570A0 (it) 1988-07-29

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