Classifications

• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
  • B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
  • B65D51/00—Closures not otherwise provided for
  • B65D51/16—Closures not otherwise provided for with means for venting air or gas
  • B65D51/1605—Closures not otherwise provided for with means for venting air or gas whereby the interior of the container is maintained in permanent gaseous communication with the exterior
  • B65D51/1622—Closures not otherwise provided for with means for venting air or gas whereby the interior of the container is maintained in permanent gaseous communication with the exterior by means of a passage for the escape of gas between the closure and the lip of the container mouth
  • B65D51/1627—Closures not otherwise provided for with means for venting air or gas whereby the interior of the container is maintained in permanent gaseous communication with the exterior by means of a passage for the escape of gas between the closure and the lip of the container mouth the closure being for a box-like container
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
  • C12M23/00—Constructional details, e.g. recesses, hinges
  • C12M23/02—Form or structure of the vessel
  • C12M23/10—Petri dish
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
  • C12M23/00—Constructional details, e.g. recesses, hinges
  • C12M23/20—Material Coatings
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
  • C12M23/00—Constructional details, e.g. recesses, hinges
  • C12M23/24—Gas permeable parts
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
  • C12M23/00—Constructional details, e.g. recesses, hinges
  • C12M23/38—Caps; Covers; Plugs; Pouring means

Definitions

• This invention concerns apparatus of the Petri dish type and a contamination-proof, prolonged-incubation sealing gasket therefor.
• One of the shortcomings of the Petri dish is that there is no control over the biological or physical characteristics of the air or gas that gets into or out of the Petri dish. Ventilation in a Petri dish is often a desirable feature and is presently provided by the "beading" of the rim of the container section if the dish is made of glass and by the presence of "ridges"
• U.S. Patents No. 3,158,353, 3,184,395, 1,513,360, and 3,326,401 The first of these describes a Petri dish fitted with a pressure-sensitive adhesive which releasably attaches the cover to the dish and hermetically seals the juncture between the dish and its cover, being removable with the cover from the dish.
• U.S. Patent No. 3,184,395 provides an envelope for aerobic culturing made of two layers of flexible, thermoplastic material having a sealing connection along the edges thereof and a plurality of heat seal partitions extending inwardly from opposite sides of the envelope, both of the layers being impervious to the culture media and at least one of the layers being pervious to air.
• Another object of this invention is to provide the plates with suitable filters to allow biological and physical control over the gases and other matter living or dead getting into ' or out of the plates, hence a greater control over the contents of the apparatus commonly referred to as the Petri dish.
• FIG. 1 is a top plan view of the gasket of the invention with parts broken away.
• Figure 2 is a cross-sectional view of the same taken along line 2-2 of Figure 1.
• Figure 3 is a longitudinal sectional view of the same taken along line 3-3 of Figure 1.
• Figure 4 is a sectional view through a Petri dish assembly incorporating the gasket of the invention.
• gasket 10 is formed of an open-celled core 12 made (e.g.) of plastic or paper having secured on at least one side a skin or membrane 14 which is permeable to gas but impermeable to bacteria and other particulate matter.
• skin 14 will cover core 12 on all -sides as shown in Figures 2, 3, and 4.
• gasket 10 will be rectangular or rounded in cross section and about 3 to 5 mm thick and wide.
• Core 12 suitably is made from soft foam sheets such as polyurethane foam a few millimeters in thickness (3 to 5 mm) with an open cell structure.
• soft foam sheets such as polyurethane foam a few millimeters in thickness (3 to 5 mm) with an open cell structure.
• suitable plastics for the core materials are available
• Skin 14 can be made from a variety of substances in the form of sheets, films, or membranes which are porous enough to allow the passage of air and other gases sufficiently to sustain the growth of microorganisms and cells inside the plate, but hinder bacteria and other microscopic particles, such as dust, pollen, etc., from gaining access to the inside of the plate.
• Such membranes cut down on the rate of evaporation from containers protected by them, since the * permeation of water vapor through them is restricted in some degree.
• Skin 14 may suitably have submicronic pores, 0.2 micron and smaller being known as absolute-sterility pores, even 0.45 micron pores being adequate to prevent contamination and allow gaseous permeation while at the same time cutting down on the rate of evaporation of water.
• These membranes hinder bacteria, spores, or other particulate matter from gaining access into the containers once the container is sealed with a suitable sealant or lubricant as the case may be.
• Skin 14 can be manufactured from a very wide variety of natural or synthetic materials, organic and inorganic (or a blend thereof) , either with a submicronic porosity or from intimately woven fibers thereof.
• Such substances may include cotton (virgin or chemically treated) , wool, artificial silk, nylon. Teflon, polyester, etc.
• Polyethylene can be used in the form of sheets of intimately woven fibers, e.g., as available from the DuPont Company under the trade name "Tyvek,” or in the form of polyethylene-coated paper having the desired porosity. Nitrocellulose is also a suitable material for such submicronic porous material sheets.
• FIG. 4 shows a Petri dish provided with a gasket 10.
• the dish can be of any shape and made of any suitable material. It includes a base 16 with a wall 18 extending upwardly therefrom. Wall 18 is formed with an upper peripheral edge 20.
• a cover 22 fits over edge 20 of the dish and extends downward loosely parallel to wall 18.
• cover 22 will have a top 24 whose ' inner peripheral surfaces 23 are adapted to seal with edge 20.
• Gasket 10 is an annulus fixed to cover 22 on the inside and to the wall 26 of cover 22 by heat sealing or by means of an adhesive such as a silicone cement or an acrylic glue. The adhesive used need not be gas permeable.
• cover 22 equipped with a circumferential "bed” on the "inside” of about 3 to 5 mm in width to hold mechanically “snugly fitting" the gasket which may be in the form of a ribbon instead of a die-cut gasket and without the need for any glue or adhesive- and without wasting of material in the die-cutting process.
• the depth of the bed may vary to match the thickness of the gasket.
• a sealant or lubricant 30 is placed on the upper edge 20 of base 16 to form a sealing engagement with gasket 10.
• the sealant can be petroleum jelly or a silicone jelly.
• Dish 10 can be manufactured in glass (with a flat brim surface, bead-free) or from plastic or polycarbonate in different heights 10, 15, 20, 25, 30 millimeters or higher and in different diameters, e.g., 60, 90, 120, 150 millimeters, etc., to suit different purposes. It is also possible to manufacture the dishes with the base divided into 2, 3, 4 sections, or more, as selected for differential diagnostic work or with a grid marking to help in differential counts.
• the dishes may be circular or square-shaped, or rectangular.
• Gasket 10 will be die cut from a sheet of selected material in the same shape as the dish.
• a particularly suitable gasket is made from a sheet of 3 to 5 mm thick filter paper (made from cellulose or fiberglass) coated with a 1 mil low-density polyethylene film having a low air permeability and low moisture transmission.
• gasket 10 has proved to cut down on the evaporation rate from culture media by approximately 50% within the growth cycle of a slowly growing microorganism, thus protecting the culture against desiccation, against drop in its pH, and against contamination from the ambient atmosphere, while at the same time protecting the ambient atmosphere and the laboratory worker from biologically hazardous materials, e.g., spores of pathogenic microrganisms.
• OMPI nutrient media and incubated under the same conditions of atmosphere, temperature duration, etc., but not equipped with the filters, the latter control plate cultures abounded with bacterial and fungal contaminant colonies.
• the nutrient medium in the dish of Figure 4 did not appreciably dry within an observation period of three weeks, while the medium in the control dish without gasket 10 shrank and dried to less than half its original size.

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• Health & Medical Sciences (AREA)
• Engineering & Computer Science (AREA)
• Organic Chemistry (AREA)
• Chemical & Material Sciences (AREA)
• Zoology (AREA)
• Life Sciences & Earth Sciences (AREA)
• Bioinformatics & Cheminformatics (AREA)
• Wood Science & Technology (AREA)
• Sustainable Development (AREA)
• Microbiology (AREA)
• Biotechnology (AREA)
• Biomedical Technology (AREA)
• Clinical Laboratory Science (AREA)
• Biochemistry (AREA)
• General Engineering & Computer Science (AREA)
• General Health & Medical Sciences (AREA)
• Genetics & Genomics (AREA)
• Immunology (AREA)
• Mechanical Engineering (AREA)
• Apparatus Associated With Microorganisms And Enzymes (AREA)

Applications Claiming Priority (1)

Publications (2)

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ID=22161500

Family Applications (1)

Country Status (4)

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• 1981
  • 1981-11-04 WO PCT/US1981/001490 patent/WO1983001581A1/en not_active Application Discontinuation
  • 1981-11-04 JP JP56503669A patent/JPS59500448A/ja active Pending
  • 1981-11-04 AU AU7891582A patent/AU7891582A/xx active Pending
  • 1981-11-04 AU AU78915/81A patent/AU7891581A/en not_active Abandoned
  • 1981-11-04 EP EP19810903170 patent/EP0092547A4/de not_active Ceased

Patent Citations (1)

Non-Patent Citations (1)

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