EA202092781A1 - Оптимизация с-5-десатурации стеролов - Google Patents

Оптимизация с-5-десатурации стеролов

Info

Publication number
EA202092781A1
EA202092781A1 EA202092781A EA202092781A EA202092781A1 EA 202092781 A1 EA202092781 A1 EA 202092781A1 EA 202092781 A EA202092781 A EA 202092781A EA 202092781 A EA202092781 A EA 202092781A EA 202092781 A1 EA202092781 A1 EA 202092781A1
Authority
EA
Eurasian Patent Office
Prior art keywords
desaturation
sterols
optimization
metabolites
derivatives
Prior art date
Application number
EA202092781A
Other languages
English (en)
Inventor
Кристофер Марк Фаррелл
Лиза Энн Лапраде
Отто Мартин Леманн
Джошуа Трухарт
Бастьен Жан Вольфганг Шеврё
Original Assignee
ДСМ АйПи АССЕТС Б.В.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ДСМ АйПи АССЕТС Б.В. filed Critical ДСМ АйПи АССЕТС Б.В.
Publication of EA202092781A1 publication Critical patent/EA202092781A1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C12P33/02—Dehydrogenating; Dehydroxylating
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/165—Yeast isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Настоящее изобретение касается усовершенствованного способа получения 7-дегидрохолестерина (7-DHC), важного промежуточного продукта при биотехнологическом получении витамина D3 или его производных/метаболитов. В изобретении представлены модифицированные штаммы клеток хозяина, экспрессирующих ферменты с улучшенной активностью C-5-стеролдесатуразы, и их применение в способе получения витамина D3 или его производных и/или метаболитов.
EA202092781A 2018-05-22 2019-05-21 Оптимизация с-5-десатурации стеролов EA202092781A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CH6292018 2018-05-22
PCT/EP2019/063079 WO2019224189A1 (en) 2018-05-22 2019-05-21 Optimization of c-5 sterol desaturation

Publications (1)

Publication Number Publication Date
EA202092781A1 true EA202092781A1 (ru) 2021-02-12

Family

ID=66770422

Family Applications (1)

Application Number Title Priority Date Filing Date
EA202092781A EA202092781A1 (ru) 2018-05-22 2019-05-21 Оптимизация с-5-десатурации стеролов

Country Status (7)

Country Link
US (1) US20210222222A1 (ru)
EP (1) EP3797168A1 (ru)
JP (1) JP7443657B2 (ru)
CN (1) CN112154213A (ru)
BR (1) BR112020023316A2 (ru)
EA (1) EA202092781A1 (ru)
WO (1) WO2019224189A1 (ru)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112813129B (zh) * 2021-02-05 2023-09-08 江南大学 利用区室化提高酵母菌中7-脱氢胆固醇产量的方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10203352A1 (de) * 2002-01-29 2003-07-31 Basf Ag Verfahren zur Herstellung von 7-Dehydrocholesterol und/oder dessen biosynthetischen Zwischen- und/oder Folgeprodukten in transgenen Organismen
WO2008130372A2 (en) * 2006-09-28 2008-10-30 Microbia, Inc. Production of sterols in oleaginous yeast and fungi
WO2011067144A1 (en) 2009-12-03 2011-06-09 Dsm Ip Assets B.V. Production of non-yeast sterols by yeast
US10082496B2 (en) * 2014-09-10 2018-09-25 Board Of Regents Of The University Of Texas System Targeting emopamil binding protein (EBP) with small molecules that induce an abnormal feedback response by lowering endogenous cholesterol biosynthesis
JP6633535B2 (ja) * 2014-10-08 2020-01-22 協和発酵バイオ株式会社 ７デヒドロコレステロール及びビタミンｄ３の製造法
WO2017051437A2 (en) * 2015-09-24 2017-03-30 Fermenta Biotech Limited A nucleotide sequence encoding 7-dehydrocholesterol reductase, recombinant constructs and recombinant organisms comprising said sequence
EP3394282B1 (en) 2015-12-23 2023-06-07 DSM IP Assets B.V. Production of sterols in modified yeast

Also Published As

Publication number Publication date
CN112154213A (zh) 2020-12-29
JP7443657B2 (ja) 2024-03-06
WO2019224189A1 (en) 2019-11-28
EP3797168A1 (en) 2021-03-31
BR112020023316A2 (pt) 2021-02-02
US20210222222A1 (en) 2021-07-22
JP2021523691A (ja) 2021-09-09

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