EA201792350A2 - Способ идентификации частично нокаутированного мутантного аллеля ind гена в биологическом образце и набор для осуществления этого способа - Google Patents

Способ идентификации частично нокаутированного мутантного аллеля ind гена в биологическом образце и набор для осуществления этого способа

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EA201792350A2
EA201792350A2 EA201792350A EA201792350A EA201792350A2 EA 201792350 A2 EA201792350 A2 EA 201792350A2 EA 201792350 A EA201792350 A EA 201792350A EA 201792350 A EA201792350 A EA 201792350A EA 201792350 A2 EA201792350 A2 EA 201792350A2
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implementing
biological sample
allee
identification
ind gene
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EA201792350A
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EA201792350A3 (ru
EA036845B1 (ru
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Бенжамен Лага
Барт Ден Бур
Барт Ламберт
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Байер Кропсайенс Н.В.
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Publication of EA201792350A2 publication Critical patent/EA201792350A2/ru
Publication of EA201792350A3 publication Critical patent/EA201792350A3/ru
Publication of EA036845B1 publication Critical patent/EA036845B1/ru

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8262Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
    • C12N15/8266Abscission; Dehiscence; Senescence
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
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    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809Methods for determination or identification of nucleic acids involving differential detection

Abstract

Это изобретение относится к сельскохозяйственным растениям, у которых изменены свойства растрескивания плода. Более конкретно, оно относится к способу идентификации частично нокаутированного мутантного аллеля IND гена в биологическом образце и к набору для осуществления этого способа. Указанный мутантный аллель обеспечивает уменьшение осыпания семян или замедляет осыпание семян до окончания сбора урожая при сохранении в то же самое время агрономически релевантной обмолачиваемости стручков, а также способствует повышению урожайности.
EA201792350A 2008-07-17 2009-07-09 Способ идентификации частично нокаутированного мутантного аллеля ind гена в биологическом образце и набор для осуществления этого способа EA036845B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13523008P 2008-07-17 2008-07-17
EP08075648 2008-07-18

Publications (3)

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EA201792350A2 true EA201792350A2 (ru) 2018-03-30
EA201792350A3 EA201792350A3 (ru) 2018-07-31
EA036845B1 EA036845B1 (ru) 2020-12-28

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EA201792350A EA036845B1 (ru) 2008-07-17 2009-07-09 Способ идентификации частично нокаутированного мутантного аллеля ind гена в биологическом образце и набор для осуществления этого способа
EA201170208A EA029333B1 (ru) 2008-07-17 2009-07-09 Растение brassica, содержащее мутантный аллель indehiscent

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EA201170208A EA029333B1 (ru) 2008-07-17 2009-07-09 Растение brassica, содержащее мутантный аллель indehiscent

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US (3) US8809635B2 (ru)
EP (2) EP3156488B1 (ru)
JP (1) JP5770086B2 (ru)
CN (2) CN102099479B (ru)
AR (1) AR072570A1 (ru)
AU (1) AU2009270539B2 (ru)
CA (2) CA2730859C (ru)
DK (1) DK3156488T3 (ru)
EA (2) EA036845B1 (ru)
ES (1) ES2604317T3 (ru)
LT (1) LT2304038T (ru)
PL (2) PL2304038T3 (ru)
PT (1) PT2304038T (ru)
SI (2) SI3156488T1 (ru)
WO (1) WO2010006732A2 (ru)

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US20200239900A1 (en) 2020-07-30
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CA2730859A1 (en) 2010-01-21
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US20140351967A1 (en) 2014-11-27
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AU2009270539B2 (en) 2015-04-23
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EP2304038A2 (en) 2011-04-06
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