EA201071017A1 - Способ получения белка человека в растении, в частности рекомбинантного лизосомного фермента человека, в эндосперме зерновых - Google Patents

Способ получения белка человека в растении, в частности рекомбинантного лизосомного фермента человека, в эндосперме зерновых

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Publication number
EA201071017A1
EA201071017A1 EA201071017A EA201071017A EA201071017A1 EA 201071017 A1 EA201071017 A1 EA 201071017A1 EA 201071017 A EA201071017 A EA 201071017A EA 201071017 A EA201071017 A EA 201071017A EA 201071017 A1 EA201071017 A1 EA 201071017A1
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EA
Eurasian Patent Office
Prior art keywords
endosperm
protein
plant
recombinant
human
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EA201071017A
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English (en)
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Стефано Марчетти
Бруно Бемби
Тамара Патти
Пьеро Кристин
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Трансактива Срл
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Publication of EA201071017A1 publication Critical patent/EA201071017A1/ru

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01045—Glucosylceramidase (3.2.1.45), i.e. beta-glucocerebrosidase
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Diabetes (AREA)
  • Cell Biology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Immunology (AREA)
  • Endocrinology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Obesity (AREA)
  • Hematology (AREA)
  • Emergency Medicine (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Epidemiology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Способ получения белка человека в растении, в частности рекомбинантного лизосомального фермента человека, в эндосперме растения, включающий первую стадию трансформации растения, посредством которой получают и заключают в эндосперме белок, который в конечном счете не поглощается зародышем, и присутствие больших количеств белка в эндосперме не оказывает отрицательного влияния на жизнеспособность и скорость прорастания семени; использование на первой стадии трансформации растения эндоспермспецифического промотора выше гена, кодирующего этот белок, и сигнального пептида для котрансляционного переноса вновь синтезированного белка в полость эндоплазматического ретикулума клеток эндосперма для его тканеспецифического накопления; вторую стадию накопления белка внутри эндосперма семени растения.
EA201071017A 2008-03-13 2009-03-11 Способ получения белка человека в растении, в частности рекомбинантного лизосомного фермента человека, в эндосперме зерновых EA201071017A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IT000055A ITUD20080055A1 (it) 2008-03-13 2008-03-13 Procedimento per la produzione di una proteina umana in pianta, in particolare un enzima lisosomiale umano ricombinante in endosperma di cereali
PCT/EP2009/052832 WO2009112508A1 (en) 2008-03-13 2009-03-11 A method for the production of a human protein in a plant, in particular a human recombinant lysosomal enzyme in a cereal endosperm

Publications (1)

Publication Number Publication Date
EA201071017A1 true EA201071017A1 (ru) 2011-06-30

Family

ID=40293330

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201071017A EA201071017A1 (ru) 2008-03-13 2009-03-11 Способ получения белка человека в растении, в частности рекомбинантного лизосомного фермента человека, в эндосперме зерновых

Country Status (19)

Country Link
US (1) US20110038971A1 (ru)
EP (1) EP2274432A1 (ru)
JP (1) JP2011516036A (ru)
KR (1) KR20100132516A (ru)
CN (1) CN102027122A (ru)
BR (1) BRPI0909336A2 (ru)
CA (1) CA2717543A1 (ru)
CO (1) CO6311018A2 (ru)
CR (1) CR11725A (ru)
EA (1) EA201071017A1 (ru)
EC (1) ECSP10010543A (ru)
GE (1) GEP20135914B (ru)
IL (1) IL208010A0 (ru)
IT (1) ITUD20080055A1 (ru)
MA (1) MA32207B1 (ru)
MX (1) MX2010010081A (ru)
NI (1) NI201000152A (ru)
NZ (1) NZ588516A (ru)
WO (1) WO2009112508A1 (ru)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ITUD20130002A1 (it) * 2013-01-16 2014-07-17 Transactiva S R L Sequenza artificiale di dna avente funzione di leader in 5' (5'-utr) ottimizzata per la sovraespressione di proteine ricombinanti in pianta e metodo per la produzione di proteine ricombinanti in pianta
KR101519402B1 (ko) * 2013-12-17 2015-05-13 강원대학교산학협력단 쌀눈 발아를 이용한 p53 항암 단백질 생산방법
CN104073492B (zh) * 2014-07-09 2016-06-22 安徽省农业科学院水稻研究所 一种作物糊粉层特异表达启动子PosAL1及其应用
KR101953950B1 (ko) * 2016-07-06 2019-05-17 전북대학교산학협력단 신호전달 펩타이드에 의한 액포수송벡터 개발
IT201700042052A1 (it) * 2017-04-14 2018-10-14 Transactiva S R L Vettore di espressione e metodo per la produzione di una proteina umana in pianta, in particolare un anticorpo ricombinante in endosperma di cereale
IT202100022157A1 (it) 2021-08-20 2023-02-20 Transactiva S R L Promotore sintetico per l’espressione di proteine eterologhe in pianta
WO2023028567A2 (en) * 2021-08-25 2023-03-02 Canbridge Pharmaceuticals, Inc. Aav particles comprising a liver-tropic capsid protein and acid alpha-glucosidase (gaa) and their use to treat pompe disease

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4251736A (en) * 1979-07-23 1981-02-17 United Technologies Corporation Method for controlling power flow between an electrochemical cell and a power grid
JPS6277833A (ja) * 1985-09-30 1987-04-10 株式会社東芝 自励式変換装置の制御方法
ZA94916B (en) * 1993-02-10 1995-08-10 Unilever Plc Immobilized proteins with specific binding capacities and their use in processes and products
US5760492A (en) * 1995-01-17 1998-06-02 Hitachi, Ltd. Control system for power transmission and distribution system
ES2326705T3 (es) 1995-09-14 2009-10-16 Virginia Tech Intellectual Properties, Inc. Produccion de enzimas lisosomicas en sistemas de expresion basados en plantas.
IL155588A0 (en) * 2003-04-27 2003-11-23 Metabogal Ltd Methods for expression of enzymatically active recombinant lysosomal enzymes in transgenic plant root cells and vectors used thereby
IT1299565B1 (it) 1998-07-17 2000-03-16 Plantechno S R L Polinucleotide sintetico codificante per la lattoferrina umana, vettori, cellule e piante transgeniche che lo contengono.
US6900619B2 (en) * 1999-11-24 2005-05-31 American Superconductor Corporation Reactive power compensation to minimize step voltage changes and transients
JP3352662B2 (ja) * 2000-02-03 2002-12-03 関西電力株式会社 二次電池システムを用いた電力系統安定化装置および電力系統安定化方法
US7417178B2 (en) * 2000-05-02 2008-08-26 Ventria Bioscience Expression of human milk proteins in transgenic plants
US20030138769A1 (en) * 2000-08-16 2003-07-24 Birkett Ashley J. Immunogenic HBc chimer particles having enhanced stability
AU2002244071A1 (en) * 2001-02-14 2002-08-28 Ventria Bioscience Expression system for seed proteins
ITRM20020115A1 (it) * 2002-03-01 2003-09-01 Plantechno S R L Espressione di enzimi lisosomiali in seme.
AU2004269200B8 (en) * 2003-08-27 2011-11-24 Orf Liftaekni Hf. Enhancing accumulation of heterologous polypeptides in plant seeds through targeted suppression of endogenous storage proteins
JP4019147B2 (ja) * 2003-10-31 2007-12-12 独立行政法人農業生物資源研究所 種子特異的プロモーターおよびその利用
US7050312B2 (en) * 2004-03-09 2006-05-23 Eaton Power Quality Corporation Multi-mode uninterruptible power supplies and methods of operation thereof
ITUD20060280A1 (it) * 2006-12-29 2008-06-30 Univ Degli Studi Udine Sequenza artificiale di dna evente funzione di leader in 5' (5'-utr) ottimizzata per la sovraespressione di proteine eterologhe in pianta

Also Published As

Publication number Publication date
BRPI0909336A2 (pt) 2018-05-22
EP2274432A1 (en) 2011-01-19
NI201000152A (es) 2011-03-24
CN102027122A (zh) 2011-04-20
GEP20135914B (en) 2013-08-26
MA32207B1 (fr) 2011-04-01
US20110038971A1 (en) 2011-02-17
IL208010A0 (en) 2010-12-30
ITUD20080055A1 (it) 2009-09-14
CO6311018A2 (es) 2011-08-22
WO2009112508A1 (en) 2009-09-17
MX2010010081A (es) 2011-03-04
JP2011516036A (ja) 2011-05-26
CR11725A (es) 2011-03-09
CA2717543A1 (en) 2009-09-17
ECSP10010543A (es) 2011-02-28
KR20100132516A (ko) 2010-12-17
NZ588516A (en) 2012-06-29

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