CO6311018A2 - Un metodo para la produccion de una proteina humana en una planta, en particular una enzima lisosomal recombinante humama en un endospermo de cereal - Google Patents

Un metodo para la produccion de una proteina humana en una planta, en particular una enzima lisosomal recombinante humama en un endospermo de cereal

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Publication number
CO6311018A2
CO6311018A2 CO10125899A CO10125899A CO6311018A2 CO 6311018 A2 CO6311018 A2 CO 6311018A2 CO 10125899 A CO10125899 A CO 10125899A CO 10125899 A CO10125899 A CO 10125899A CO 6311018 A2 CO6311018 A2 CO 6311018A2
Authority
CO
Colombia
Prior art keywords
endosperm
protein
enzyme
human
plant
Prior art date
Application number
CO10125899A
Other languages
English (en)
Inventor
Stefano Marchetti
Bruno Bembi
Tamara Patti
Piero Cristin
Original Assignee
Transactiva Srl
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Transactiva Srl filed Critical Transactiva Srl
Publication of CO6311018A2 publication Critical patent/CO6311018A2/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01045—Glucosylceramidase (3.2.1.45), i.e. beta-glucocerebrosidase
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Diabetes (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Immunology (AREA)
  • Emergency Medicine (AREA)
  • Hematology (AREA)
  • Obesity (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Endocrinology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Epidemiology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Un método para la producción de una proteína humana en una planta, en particular de una enzima lisosomal humana recombinante en un endospermo de una planta de cereal, que comprende:- un primer paso de transformación de la planta de cereal en donde la enzima lisosomal de proteína es obtenida y confinada en un endospermo, que eventualmente no es absorbido por el embrión, y la presencia de grandes cantidades de la enzima lisosomal de proteína en el endospermo no afecta de forma negativa la viabilidad de las semillas y la velocidad de germinación;- el uso, en el primer paso de la transformación de la planta de cereal, de un promotor especifico de endospermo corriente arriba del gen que codifica dicha enzima isosomal de proteína, y de un péptido de señal para una transferencia cotraslacional de la enzima lisosomal de proteína recientemente sintetizada dentro del lumen del retículo endoplasmático de las células del endospermo para su acumulación especifica a tejidos;- un segundo paso de acumulación de la enzima lisososmal dentro del endospermo de la semilla de una planta de cereal.
CO10125899A 2008-03-13 2010-10-11 Un metodo para la produccion de una proteina humana en una planta, en particular una enzima lisosomal recombinante humama en un endospermo de cereal CO6311018A2 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IT000055A ITUD20080055A1 (it) 2008-03-13 2008-03-13 Procedimento per la produzione di una proteina umana in pianta, in particolare un enzima lisosomiale umano ricombinante in endosperma di cereali

Publications (1)

Publication Number Publication Date
CO6311018A2 true CO6311018A2 (es) 2011-08-22

Family

ID=40293330

Family Applications (1)

Application Number Title Priority Date Filing Date
CO10125899A CO6311018A2 (es) 2008-03-13 2010-10-11 Un metodo para la produccion de una proteina humana en una planta, en particular una enzima lisosomal recombinante humama en un endospermo de cereal

Country Status (19)

Country Link
US (1) US20110038971A1 (es)
EP (1) EP2274432A1 (es)
JP (1) JP2011516036A (es)
KR (1) KR20100132516A (es)
CN (1) CN102027122A (es)
BR (1) BRPI0909336A2 (es)
CA (1) CA2717543A1 (es)
CO (1) CO6311018A2 (es)
CR (1) CR11725A (es)
EA (1) EA201071017A1 (es)
EC (1) ECSP10010543A (es)
GE (1) GEP20135914B (es)
IL (1) IL208010A0 (es)
IT (1) ITUD20080055A1 (es)
MA (1) MA32207B1 (es)
MX (1) MX2010010081A (es)
NI (1) NI201000152A (es)
NZ (1) NZ588516A (es)
WO (1) WO2009112508A1 (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ITUD20130002A1 (it) * 2013-01-16 2014-07-17 Transactiva S R L Sequenza artificiale di dna avente funzione di leader in 5' (5'-utr) ottimizzata per la sovraespressione di proteine ricombinanti in pianta e metodo per la produzione di proteine ricombinanti in pianta
KR101519402B1 (ko) * 2013-12-17 2015-05-13 강원대학교산학협력단 쌀눈 발아를 이용한 p53 항암 단백질 생산방법
CN104073492B (zh) * 2014-07-09 2016-06-22 安徽省农业科学院水稻研究所 一种作物糊粉层特异表达启动子PosAL1及其应用
KR101953950B1 (ko) * 2016-07-06 2019-05-17 전북대학교산학협력단 신호전달 펩타이드에 의한 액포수송벡터 개발
IT201700042052A1 (it) * 2017-04-14 2018-10-14 Transactiva S R L Vettore di espressione e metodo per la produzione di una proteina umana in pianta, in particolare un anticorpo ricombinante in endosperma di cereale
IT202100022157A1 (it) 2021-08-20 2023-02-20 Transactiva S R L Promotore sintetico per l’espressione di proteine eterologhe in pianta
EP4392570A2 (en) * 2021-08-25 2024-07-03 CANbridge Pharmaceuticals, Inc. Aav particles comprising liver-tropic capsid protein and acid alpha-glucosidase and use to treat pompe disease

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IL155588A0 (en) * 2003-04-27 2003-11-23 Metabogal Ltd Methods for expression of enzymatically active recombinant lysosomal enzymes in transgenic plant root cells and vectors used thereby
IT1299565B1 (it) 1998-07-17 2000-03-16 Plantechno S R L Polinucleotide sintetico codificante per la lattoferrina umana, vettori, cellule e piante transgeniche che lo contengono.
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ITRM20020115A1 (it) * 2002-03-01 2003-09-01 Plantechno S R L Espressione di enzimi lisosomiali in seme.
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JP4019147B2 (ja) * 2003-10-31 2007-12-12 独立行政法人農業生物資源研究所 種子特異的プロモーターおよびその利用
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Also Published As

Publication number Publication date
ECSP10010543A (es) 2011-02-28
NI201000152A (es) 2011-03-24
EA201071017A1 (ru) 2011-06-30
MX2010010081A (es) 2011-03-04
CA2717543A1 (en) 2009-09-17
MA32207B1 (fr) 2011-04-01
JP2011516036A (ja) 2011-05-26
BRPI0909336A2 (pt) 2018-05-22
EP2274432A1 (en) 2011-01-19
WO2009112508A1 (en) 2009-09-17
US20110038971A1 (en) 2011-02-17
NZ588516A (en) 2012-06-29
IL208010A0 (en) 2010-12-30
ITUD20080055A1 (it) 2009-09-14
KR20100132516A (ko) 2010-12-17
CN102027122A (zh) 2011-04-20
GEP20135914B (en) 2013-08-26
CR11725A (es) 2011-03-09

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