EA200800091A1 - Белок, выделенный из растений рода lupinus или полученный в рекомбинантной форме, кодирующая его последовательность нуклеотидов и его применение в питании животных, в качестве стимулятора роста растений и при борьбе с патогенными грибами - Google Patents

Белок, выделенный из растений рода lupinus или полученный в рекомбинантной форме, кодирующая его последовательность нуклеотидов и его применение в питании животных, в качестве стимулятора роста растений и при борьбе с патогенными грибами

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Publication number
EA200800091A1
EA200800091A1 EA200800091A EA200800091A EA200800091A1 EA 200800091 A1 EA200800091 A1 EA 200800091A1 EA 200800091 A EA200800091 A EA 200800091A EA 200800091 A EA200800091 A EA 200800091A EA 200800091 A1 EA200800091 A1 EA 200800091A1
Authority
EA
Eurasian Patent Office
Prior art keywords
protein
plants
plant growth
recombinant form
stimulant
Prior art date
Application number
EA200800091A
Other languages
English (en)
Other versions
EA012336B1 (ru
Inventor
Рикарду Мануэл Ди Сейшаш Буавида Феррейра
Сара Алекшандра Валадаш Да Силва Монтейру
Артур Рикарду Нашсименту Тейшейра
Виржилиу Боржиш Лорейру
Original Assignee
Инштитуто Супериор Ди Агрономия
Рикарду Мануэл Ди Сейшаш Буавида Феррейра
Валадаш Да Силва Монтейру, Сара Александра
Артур Рикарду Нашсименту Тейшейра
Виржилиу Боржиш Лорейру
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PT10332205A external-priority patent/PT103322A/pt
Priority claimed from PT10351106A external-priority patent/PT103511A/pt
Application filed by Инштитуто Супериор Ди Агрономия, Рикарду Мануэл Ди Сейшаш Буавида Феррейра, Валадаш Да Силва Монтейру, Сара Александра, Артур Рикарду Нашсименту Тейшейра, Виржилиу Боржиш Лорейру filed Critical Инштитуто Супериор Ди Агрономия
Publication of EA200800091A1 publication Critical patent/EA200800091A1/ru
Publication of EA012336B1 publication Critical patent/EA012336B1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A01N65/20—Fabaceae or Leguminosae [Pea or Legume family], e.g. pea, lentil, soybean, clover, acacia, honey locust, derris or millettia
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A23K20/147—Polymeric derivatives, e.g. peptides or proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A23L33/18—Peptides; Protein hydrolysates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/02—Nutrients, e.g. vitamins, minerals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8282—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Polymers & Plastics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Mycology (AREA)
  • Nutrition Science (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Agronomy & Crop Science (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Environmental Sciences (AREA)
  • Dentistry (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Animal Husbandry (AREA)
  • Hematology (AREA)
  • Veterinary Medicine (AREA)
  • Obesity (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)

Abstract

Данное изобретение относится к экстракции белка из семян, семядолей или проростков рода Lupinus, а также к способу его получения в рекомбинантной форме и его экспрессии в генетически модифицированных растениях. Благодаря превосходным характеристикам, проявляемым этим белком, в отношении: 1) его сильной противогрибковой и противооомицетной активности, которая наделяет этот белок высоким потенциалом в качестве фунгицида, 2) его высокой активности, стимулирующей рост растений, в частности, известной в отношении нездоровых или естественно ослабленных растений, 3) его исключительной устойчивости к денатурации, которая позволяет применять этот белок в полевых условиях, 4) его высокой чувствительности к протеолизу, что делает его безвредным для окружающей среды и нетоксичным для человека и 5) его хорошо сбалансированного аминокислотного состава. Заявлено его применение или применение любой модификации этого белка, которая сохраняет его биологические свойства, в качестве добавки к пище человека или животных и в качестве фунгицида, инсектицида, стимулятора роста, удобрения или при получении генетически модифицированных организмов.
EA200800091A 2005-07-21 2006-07-13 Белок, выделенный из растений рода lupinus или полученный в рекомбинантной форме, кодирующая его последовательность нуклеотидов и его применение в питании животных, в качестве стимулятора роста растений и при борьбе с патогенными грибами EA012336B1 (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
PT10332205A PT103322A (pt) 2005-07-21 2005-07-21 Proteína extraída de plantas do género lupinus, sequência nucleotídica que a codifica e sua utilização no combate a fungos patogénicos por aplicação directa, na forma recombinante ou por expressão em plantas transgénicas
PT10351106A PT103511A (pt) 2006-06-28 2006-06-28 Utilização de uma proteína de plantas do género lupinus como bioestimulante do crescimento e desenvolvimento de plantas e na formulação de preparados proteicos para alimentação humana e/ou animal
PCT/IB2006/052403 WO2007010459A2 (en) 2005-07-21 2006-07-13 Protein extracted from plants of the genus lupinus or produced in recombinant form, nucleotide sequence encoding it and its use in animal nutrition, as a plant growth promoter and in the fight against pathogenic fungi

Publications (2)

Publication Number Publication Date
EA200800091A1 true EA200800091A1 (ru) 2008-08-29
EA012336B1 EA012336B1 (ru) 2009-08-28

Family

ID=37669208

Family Applications (1)

Application Number Title Priority Date Filing Date
EA200800091A EA012336B1 (ru) 2005-07-21 2006-07-13 Белок, выделенный из растений рода lupinus или полученный в рекомбинантной форме, кодирующая его последовательность нуклеотидов и его применение в питании животных, в качестве стимулятора роста растений и при борьбе с патогенными грибами

Country Status (18)

Country Link
US (3) US8106252B2 (ru)
EP (3) EP2333086B1 (ru)
JP (1) JP5080467B2 (ru)
CN (2) CN101365793B (ru)
AU (1) AU2006271282B2 (ru)
BR (1) BRPI0613746B1 (ru)
CA (1) CA2617052C (ru)
CY (3) CY1113751T1 (ru)
DK (3) DK2333086T3 (ru)
EA (1) EA012336B1 (ru)
ES (3) ES2534352T3 (ru)
HU (1) HUE025372T2 (ru)
IL (1) IL188907A (ru)
NZ (1) NZ565242A (ru)
PL (3) PL2221383T3 (ru)
PT (3) PT1907550E (ru)
SI (3) SI2333086T1 (ru)
WO (1) WO2007010459A2 (ru)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2333086B1 (en) * 2005-07-21 2015-01-07 Instituto Superior de Agronomia Use of a lupinus protein
GB2484509A (en) * 2010-10-13 2012-04-18 Cev Biotecnologia Das Plantas S A Lupin beta-conglutin derived protein fragment Blad as an antimicrobial agent
PT105331A (pt) * 2010-10-12 2012-04-12 Cev Biotecnologia Das Plantas S A Conservante alimentar
PT105332A (pt) * 2010-10-12 2012-04-12 Cev Biotecnologia Das Plantas S A Agentes para utilização com antimicrobianos
WO2012049215A1 (en) * 2010-10-12 2012-04-19 Consumo Em Verde - Biotecnologia Das Plantas, S.A. Antimicrobial protein
EP2630475B1 (en) 2010-10-21 2020-06-10 Instituto Superior de Agronomia Diagnostic method and treatment
US9150812B2 (en) 2012-03-22 2015-10-06 Exxonmobil Research And Engineering Company Antioxidant combination and synthetic base oils containing the same
FR3023452B1 (fr) 2014-07-11 2016-07-08 Prp Holding Utilisation d'une composition organo-minerale pour augmenter l'assimilation des elements nutritifs du sol par la plante
MX2017008728A (es) 2014-12-29 2017-10-31 Fmc Corp Composiciones microbianas y metodos de uso para favorecer el crecimiento de las plantas y tratar las enfermedades de las plantas.
US10888090B2 (en) 2015-06-30 2021-01-12 King Abdullah University Of Science And Technology Plant growth promoters and methods of using them
BR112019006174A2 (pt) * 2016-09-30 2019-06-18 Inst Superior Agronomia proteina terapêutica
CN108477185A (zh) * 2018-04-19 2018-09-04 张月婵 含有β-羽扇豆球蛋白多肽和二氯噁菌唑的杀菌组合物
CN108633921A (zh) * 2018-04-19 2018-10-12 张友兰 含有β-羽扇豆球蛋白多肽和啶菌噁唑的杀菌组合物
CN108378069A (zh) * 2018-05-09 2018-08-10 惠州市梦芭迪工业产品设计中心(普通合伙) 一种杀菌组合物
PT115569A (pt) * 2019-06-06 2021-04-06 Inst Superior Agronomia Uso de um adaptógeno para tratamento de plantas e método respetivo

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8724419D0 (en) 1987-10-19 1987-11-25 Favor Parker Ltd Animal feed products
DK0392225T3 (da) 1989-03-24 2003-09-22 Syngenta Participations Ag Sygdomsresistente transgene planter
GB9807256D0 (en) * 1998-04-03 1998-06-03 Du Pont Uk Functional protein compositions,emulsions based thereon and processes for their preparation
EP2333086B1 (en) * 2005-07-21 2015-01-07 Instituto Superior de Agronomia Use of a lupinus protein

Also Published As

Publication number Publication date
AU2006271282B2 (en) 2011-03-24
DK2333086T3 (en) 2015-04-07
EP1907550A2 (en) 2008-04-09
ES2540109T3 (es) 2015-07-08
PL1907550T3 (pl) 2013-03-29
PT2221383E (pt) 2015-07-24
US8541654B2 (en) 2013-09-24
US20140007303A1 (en) 2014-01-02
ES2396722T3 (es) 2013-02-25
EP2221383A1 (en) 2010-08-25
NZ565242A (en) 2014-06-27
CA2617052A1 (en) 2007-01-25
US20110088128A1 (en) 2011-04-14
IL188907A (en) 2014-12-31
PL2221383T3 (pl) 2015-09-30
US20080300137A1 (en) 2008-12-04
CY1116269T1 (el) 2017-02-08
CN103314984A (zh) 2013-09-25
SI2333086T1 (sl) 2015-07-31
PT1907550E (pt) 2013-01-23
CN101365793A (zh) 2009-02-11
AU2006271282A1 (en) 2007-01-25
CN103314984B (zh) 2015-06-03
US8106252B2 (en) 2012-01-31
DK1907550T3 (da) 2012-12-03
WO2007010459A2 (en) 2007-01-25
BRPI0613746A2 (pt) 2011-02-08
HUE025372T2 (en) 2016-02-29
CY1116468T1 (el) 2017-03-15
JP2009505637A (ja) 2009-02-12
PT2333086E (pt) 2015-04-28
IL188907A0 (en) 2008-04-13
EP2333086A1 (en) 2011-06-15
JP5080467B2 (ja) 2012-11-21
BRPI0613746B1 (pt) 2017-11-07
WO2007010459A3 (en) 2007-10-11
ES2534352T3 (es) 2015-04-21
SI2221383T1 (sl) 2015-10-30
EP1907550B1 (en) 2012-10-17
EA012336B1 (ru) 2009-08-28
CN101365793B (zh) 2013-05-22
EP2333086B1 (en) 2015-01-07
US9382300B2 (en) 2016-07-05
PL2333086T3 (pl) 2015-08-31
CY1113751T1 (el) 2016-07-27
WO2007010459B1 (en) 2008-08-21
CA2617052C (en) 2016-10-04
DK2221383T3 (en) 2015-06-29
SI1907550T1 (sl) 2013-02-28
EP2221383B1 (en) 2015-03-25

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