EA032369B1 - Method for treating inflammatory diseases of the oral mucosa associated with concomitant chronic pathology - Google Patents

Abstract

The invention relates to medicine, in particular to the treatment of diseases of the oral organs such as catarrhal gingivitis, disease of salivary glands, herpetic stomatitis, ulcerative diseases of the oral mucosa and other inflammatory diseases of the oral soft tissues. The essence of the invention consists in a method for enhancing the local immunity in diseases of the oral organs. The method includes the local therapeutic treatment associated with concomitant chronic pathology, standard hygiene and sanation of the oral cavity with the influence on the oral tissues exerted by stimulated autoleukocytes obtained from the patient's blood. The use of autologous products obtained in the process of patient's leukocytes culturing reduces the terms of wound healing, stimulates granulations. This method can be used for the treatment of a wide range of diseases associated with the immune system disorders.

Description

The invention relates to medicine, namely to the treatment of diseases of the oral organs, such as catarrhal gingivitis, salivary gland disease, herpetic stomatitis, ulcerative diseases of the oral mucosa and other inflammatory diseases of the soft tissues of the oral cavity. The invention consists in a method of increasing local immunity in diseases of the organs of the oral cavity. The method provides local therapeutic treatment against a background of concomitant chronic pathology, standard hygiene and sanitation of the oral cavity, followed by exposure of the oral tissue to a solution of stimulated autoleukocytes obtained from the patient’s blood. The use of autologous preparations obtained during the cultivation of the patient’s white blood cells significantly reduces the healing time of wounds, stimulates the appearance of granulations. This method can be used in the treatment of a wide range of diseases in which there are disorders in the immune system.

The invention relates to medicine, namely to the treatment of diseases of the oral organs, such as catarrhal gingivitis, salivary gland disease, herpetic stomatitis, ulcerative diseases of the oral mucosa and other inflammatory diseases of the soft tissues of the oral cavity.

Known methods for treating diseases such as catarrhal gingivitis, salivary gland disease, herpetic stomatitis and other ulcers of the oral mucosa. In the treatment of these diseases are used, as a rule, for each specific type of disease, a set of generally accepted standard tools and techniques. Most of the drugs used weaken the local immune system, which affects the duration of treatment and frequent relapses. In addition, it is known that diseases of the organs of the oral cavity are, as a rule, a reflection of the internal problems of the whole organism, which may be the result of problems with the gastrointestinal tract, various kinds of anemia and vitamin deficiency, endocrine problems, allergic reactions, and sometimes even more serious and dangerous diseases that weaken the immune system of the whole organism, and drugs used in the treatment of diseases of the oral organs: antibiotics, antiseptics upset the balance of microflora in the oral cavity and interfere with the formation of the body's immune responses to pathogenic bacteria. One of the effective directions in this regard is the immunological way of solving the problem with the use of treatment methods that stimulate immunological reactivity and increase the nonspecific resistance of the body. From literary sources it is known that the use of antiviral drugs or immunocorrective therapy or vaccine therapy is not always effective in treating oral diseases, especially in patients with chronic diseases in this area, as well as in patients with concomitant pathology of other internal organs. For the appointment of immunocorrective therapy, complex immunological studies are necessary. In addition, many immunostimulants have a large number of side effects. Therefore, methods are currently being developed that use a new approach in the treatment of wounds of various origins, including diseases of the organs of the oral cavity - using autologous drugs - self-activated patients own blood cells. The production method and methods for their use are quite diverse. It was found that the use of autologous drugs obtained from the patient’s white blood cells improves the patient’s immune condition, which significantly reduces the healing time of wounds, prolongs remission and reduces relapses.

Known (1) is a method for the prevention and treatment of diseases of the oral cavity, which provides for the stabilization of the immune system with an aqueous or physiological solution containing an ozone-oxygen mixture, the solution being obtained by sparging through water or a physiological solution of an ozone-oxygen mixture. The method is intended for the treatment of certain diseases of the oral cavity, including catarrhal gingivitis. This method allows you to get a good therapeutic effect, but only for chronic patients and not complicated by other pathologies.

Also known (2) is a method for treating oral diseases using autostimulated leukocytes obtained by magnetizing autologous blood for the treatment of chronic herpetic stomatitis. However, this method is aimed at treating the disease without concomitant pathologies.

The disadvantage of all known solutions is that they are aimed at treating a specific organ of the disease using a set of standard drugs without any connection with diseases of internal organs and body systems that are prone to chronic diseases.

Closest to the claimed method is the well-known (3) method for treating pathological foci of diseases of the oral cavity of various origins, including periodontitis of moderate severity using autostimulated leukocytes, which were obtained by isolating leukocytes from peripheral blood and stimulating them with mitogen. The method provides the prevention of secondary immunodeficiency, reduces inflammation, cleanses the wound from microorganisms, stimulates repair, and reduces the dose and timing of taking antibiotics. The disadvantage of this method is that the method is intended for the treatment of diseases of moderate severity, not burdened by chronic or concomitant diseases. To obtain autostimulated leukocytes, at least 15 ml of blood is taken from the patient each time, the selection of leukocytes and the method of stimulation are quite a long and complicated process, and the use of the obtained autostimulated leukocytes occurs, as a rule, by traumatic invasive methods.

The objective of the invention is to develop a method for the treatment of diseases of the organs of the oral cavity, which are interrelated with other diseases of the body, allowing by increasing local immunity to achieve a stable therapeutic effect.

The essence of the invention lies in a method for treating inflammatory diseases of the oral mucosa, against a background of concomitant chronic pathology, including standard hygienic sanitation of the oral mucosa and exposure of the oral mucosa to a solution containing autoleukocytes isolated by centrifugation of blood obtained from the patient’s ulnar vein, and stimulated by adding an immunophen solution in an amount of 0.1 to 5 ml of the obtained white blood cell sample after centrifugation and subsequent her incubation in an thermostat for an hour at a temperature of 37 ° C, to which saline is added in the ratio of autolocytes:

- 1 032369 saline as 1: 3, respectively.

With herpetic stomatitis, a solution of stimulated autoleukocytes is used in the form of triple oral baths with an interval of 10 minutes and an exposure of 4-6 minutes. Each, with a course of treatment of at least 10 procedures every other day.

In catarrhal gingivitis, a solution of stimulated autoleukocytes is applied to a sterile cotton swab and applied to the papillary-marginal-alveolar part of the gums with a half-hour exposure and a course of treatment of at least three procedures.

In case of disease of the salivary gland, at the end of the acute period (excreted saliva does not contain pus), irrigation of the ducts of the gland with a solution of stimulated autoleukocytes with a course of treatment of at least three procedures in 1-2 days is prescribed.

A comparative analysis of the claimed invention with known methods in this field showed that the claimed invention differs from the known ones in that the method provides a comprehensive treatment of diseases of the oral cavity, taking into account concomitant and recurrent diseases of the body. Autoimmune leukocytes taken from the patient’s blood and autostimulated by the immunomodulator immunofan are used to improve local immunity. Treatment with stimulated autoleukocytes is carried out according to the method developed by the inventors for each type of disease.

In the claimed invention, autostimulated leukocytes are prepared according to the well-known (4), but slightly modified technique.

In patients in the morning, 5 ml of blood is taken from the cubital vein in the morning and placed in a test tube with heparin. After centrifugation at 1,500 rpm, the upper two fractions, the blood plasma and the white blood cell layer, are taken from the test tubes with a microdoser. Immunofan solution is added to the obtained samples in the amount of: 0.1 ml per 5 ml of blood and incubated in a test tube for an hour in an incubator at 37 ° C. After incubation, physiological saline is added in the ratio of autoleukocytes: saline - 1: 3. The resulting autostimulated white blood cells are divided into three aliquots and stored until used in the refrigerator. Depending on the type of disease, a technique has been developed for the use of autostimulated white blood cells.

The implementation of the proposed method and the results of its application is illustrated by the following examples.

Example 1

Patient V.M., 64 years old, has been on hemodialysis for 5 years with a diagnosis of end-stage chronic kidney disease and type 2 diabetes. During a dental examination revealed: hyperemia, swelling of the oral mucosa, the presence of supragingival and subgingival dental deposits, seizures at the corners of the mouth, tongue coating, plaque inflammation. The patient was diagnosed with mild catarrhal gingivitis. General therapeutic treatment was prescribed in accordance with the concomitant disease, standard high-quality oral hygiene was carried out, and the treatment procedure for the patient with stimulated autoleukocytes was determined.

Following the rules of antiseptics, a solution of stimulated autoleukocytes was applied to a sterile cotton swab and applied to the papillary-marginal-alveolar part of the gums. Medical tampons were prepared immediately before application. Oral applications were kept for about half an hour. After complex treatment, the dental PMA index was 25.0%, and the pH was 1.5. After treatment, the hygienic condition of the oral cavity improved, swelling and hyperemia of the oral mucosa decreased.

Patient performance before treatment:

KPU - 9; RMA - 403%, PHP - 2; Immunological parameters in saliva: IL -1β - 64.3 pg / ml; IL-2 26.7 pg / ml; IFN-γ - 13.2 pg / ml.

Patient performance after occupational hygiene and antiseptic therapy:

KPU - 9; RMA - 27.5%, PHP - 1.5; Immunological parameters in saliva: IL -1β - 44.3 pg / ml; IL-2 -19.4 pg / ml; IFN-γ -17.1 pg / ml.

Example 2

Patient A.E., 75 years old, has been on program hemodialysis for 8 years with a diagnosis of end-stage chronic kidney disease and type 2 diabetes. During a dental examination revealed: hyperemia, swelling of the oral mucosa, the presence of supragingival and subgingival dental deposits, seizures at the corners of the mouth, tongue coating, plaque inflammation. The patient was diagnosed with a catarrhal gingivitis of moderate severity. General therapeutic treatment was prescribed and standard quality oral hygiene was carried out.

The patient underwent 3 application procedures with stimulated autoleukocytes. Following the rules of antiseptics, a solution of autoleukocyte suspension was applied to a sterile cotton swab and applied to the papillary-marginal-alveolar part of the gums. Medical tampons were prepared immediately before application. Oral applications were kept for about half an hour. After

- 2 032369 of the complex treatment, the dental index of PMA was 25.0%, and the pH was 1.5. After treatment, the hygienic condition of the oral cavity improved, swelling and hyperemia of the oral mucosa decreased.

Patient performance before treatment:

KPU - 10; RMA - 30.5%, pH -1.8; Immunological parameters in saliva: ΗΠ-1β-53.2 pg / ml; IL-2 25.6 pg / ml; IFN-γ - 9.1 pg / ml.

Patient performance after occupational hygiene and antiseptic therapy:

KPU - 12; RMA - 28.9%, pH - 1.6: Immunological parameters in saliva: IL -1β - 51.2 pg / ml; IL2 -213 pg / ml; IFN-γ - 13.5 pg / ml.

Example 3

Patient A.M. 1970, suffers from ulcerative chronic stomatitis. Was admitted to the hospital with a diagnosis of acute (ulcerative) herpetic stomatitis. General therapeutic treatment was prescribed and standard quality oral hygiene was carried out. 3-5 minutes after oral hygiene, the mouth was irrigated with normal running water, and then 3-5 minutes later, stimulated autoleukocytes in the form of oral baths with pauses were used. The treatment included 10 treatments every other day. After 20 days of treatment, the oral mucosa was completely cleared of the lesion. After 20 days, a second rash began, but with less intensity than initially. Re-applied stimulated autolekocytes by the same technique, but only 7 procedures every other day. Repeated relapse occurred after 2 months. The treatment was repeated according to the same scheme, but already 5 procedures every other day. The patient is in remission to the present (more than 6 months).

Patient performance before treatment:

Immunological parameters in saliva: IL - 1β -63.1 pg / ml; IL-2 - 20.9 pg / ml; IFN-γ - 4.8 pg / ml.

Patient indicators after professional hygiene and antiseptic therapy: Immunological parameters in saliva: IL - 1β -45.5 pg / ml; IL-2 - 23.8 pg / ml; IFN-γ - 15.3 pg / ml. Example 4

Patient T.Z., 1961 was admitted to the hospital with a diagnosis of herpetic (ulcerative) stomatitis with somatic pathology of chronic cholecystitis, multiple ulcers of the duodenum, acute hyperocidal gastritis. General therapeutic treatment of pathologies and treatment of ulcerative stomatitis with stimulated autoleukocytes were prescribed. Previously, a qualitative rehabilitation of the oral cavity was carried out. 3-5 minutes after the sanitation of the oral cavity, the mouth was irrigated with ordinary running water, and then 3-5 minutes later, stimulated autoleukocytes in the form of oral baths with an exposure of 5 minutes were used. This procedure was repeated three times with 10-minute pauses. The treatment included 10 treatments every other day. After these procedures, the patient's health was restored. Remission lasted for 1 month. The new firing was less intense. Re-applied stimulated autoleukocytes by the same method, but only 7 procedures every other day and repeated therapeutic treatment of these pathologies. Remission lasted 2 months. Repeated exacerbation was negligible. The treatment was repeated according to the same scheme, but already 5 procedures every other day. The patient is in remission to the present (more than 7 months).

Patient performance before treatment:

Immunological parameters in saliva: IL -1β - 65.2 pg / ml; IL-2 - 22.8 pg / ml; IFN-γ - 4.5 pg / ml. Patient performance after occupational hygiene and antiseptic therapy:

Immunological parameters in saliva: IL - 1β - 43.2 pg / ml; IL-2 - 22.1 pg / ml; IFN-γ - 14.3 pg / ml. Example 5

Patient B.S. Born in 1980, she was admitted to the hospital with a diagnosis of chronic sialodenitis of the right parotid gland. Visually: swelling, suppuration from the duct of the gland and pain. General treatment and washing of the ducts with antiseptic and antibacterial agents has been prescribed. At the end of the acute period after the cessation of purulent secretions, a solution of 1 ml of stimulated autoleukocytes in 1 ml of saline was injected into the ducts of the salivary gland. Long remission.

Patient performance before treatment:

Immunological parameters in saliva: ΠΠ-1β - 60.2 pg / ml; IL-2 - 26.0 pg / ml; IFN-γ - 9.1 pg / ml.

Patient indicators after professional hygiene and antiseptic therapy: Immunological parameters in saliva: ΠΠ-1β -50.4 pg / ml; IL-2 - 23.4 pg / ml; IFN-γ - 10.2 pg / ml. Example 6

Patient M.Z. Born in 1957, she was admitted to the hospital with a diagnosis of chronic sialodenitis of the left parotid gland. Visually: swelling of the parotid gland, redness, suppuration from the duct of the gland, and pain. At the end of the acute period after the cessation of purulent secretions, a solution of 1 ml of stimulated autoleukocytes in 1 ml of saline was injected into the ducts of the salivary gland. Long remission.

Patient performance before treatment:

Immunological parameters in saliva: ΠΠ-1β-61.3 pg / ml; IL-2 - 27.4 pg / ml; IFN-γ - 8.8 pg / ml.

Patient performance after occupational hygiene and antiseptic therapy:

Immunological parameters in saliva: IL - 1β - 48.2 pg / ml; IL-2 - 25.0 pg / ml; IFN-γ - 10.9 pg / ml.

- 3 032369

The technical effect of the claimed invention consists in solving the problem, namely, a methodology has been developed for the complex treatment of inflammatory diseases of the oral cavity, complicated by chronic and concomitant pathologies using autostimulated leukocytes. The application of the proposed method significantly reduces the healing time of wounds, stimulates the appearance of granulations. This method can be used in the treatment of a wide range of diseases in which there are disorders in the immune system.

Literature

1) KI No. 2146917 from 01/16/1998, A method for the prevention and treatment of diseases of the oral cavity.

2) KI No. 9401733 dated 05/10/1994, A method for the treatment of chronic herpetic stomatitis.

3) KI No. 2414228 of 11/19/2009, Method of personified immunotherapy, (prototype)

4) KI No. 2403071 dated 03.09.2009, A method for the treatment of chronic recurrent inflammatory diseases of the nasal mucosa and paranasal sinuses using endonasal autolymphocytotherapy.

Claims (4)

CLAIM 1. A method of treating inflammatory diseases of the oral mucosa against a background of concomitant chronic pathology, including standard hygienic sanitation of the oral mucosa and exposure of the oral mucosa to a solution containing autoleukocytes isolated by centrifugation of blood obtained from the patient’s cubital vein and stimulated by adding Immunofen solution in an amount of 0.1 ml per 5 ml of the obtained leukocyte sample after centrifugation and subsequent incubation in an incubator for 1 h at 37 ° C, to which was added physiological saline at a ratio autoleykotsity: saline 1: 3, respectively. 2. The method according to claim 1, characterized in that with herpetic stomatitis, a solution of stimulated autoleukocytes acts on the inflammatory focus in the form of triple oral baths with an interval of 10 minutes and an exposure of 4-6 minutes each, with a course of treatment of at least 10 procedures in one day. 3. The method according to claim 1, characterized in that with catarrhal gingivitis, a solution of stimulated autoleukocytes is applied by applying it to a sterile cotton swab, which is applied to the papillary-marginal-alveolar part of the gums with a half-hour exposure, while the course of treatment is at least three procedures . 4. The method according to claim 1, characterized in that in case of salivary gland disease, a solution of stimulated autoleukocytes is applied at the end of the acute period in the form of irrigation of the ducts of the salivary gland, and the course of treatment is at least three procedures in 1-2 days.