EA029209B1 - Кластер генов биосинтеза гризелимицина и метилгризелимицина - Google Patents

Кластер генов биосинтеза гризелимицина и метилгризелимицина Download PDF

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Publication number
EA029209B1
EA029209B1 EA201490767A EA201490767A EA029209B1 EA 029209 B1 EA029209 B1 EA 029209B1 EA 201490767 A EA201490767 A EA 201490767A EA 201490767 A EA201490767 A EA 201490767A EA 029209 B1 EA029209 B1 EA 029209B1
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Eurasian Patent Office
Prior art keywords
protein
nucleic acid
proteins
methyl
variant
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EA201490767A
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English (en)
Russian (ru)
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EA201490767A1 (ru
Inventor
Марк БРЕНСТРУП
Каудиа Кениг
Луиджи Тоти
Йоахим ВИНК
Вульф Лойшнер
Йоханн ГАССЕНХУБЕР
Рольф Мюллер
Зильке Венцель
Тина Бинц
Карстен Фольц
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Санофи
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Priority claimed from EP11306400.0A external-priority patent/EP2586791A1/en
Application filed by Санофи filed Critical Санофи
Publication of EA201490767A1 publication Critical patent/EA201490767A1/ru
Publication of EA029209B1 publication Critical patent/EA029209B1/ru

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/36—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Actinomyces; from Streptomyces (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against enzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/64—Cyclic peptides containing only normal peptide links
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/24—Proline; Hydroxyproline; Histidine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/18—Testing for antimicrobial activity of a material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/25—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving enzymes not classifiable in groups C12Q1/26 - C12Q1/66
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/465—Streptomyces

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Toxicology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Pyrrole Compounds (AREA)
EA201490767A 2011-10-12 2012-10-11 Кластер генов биосинтеза гризелимицина и метилгризелимицина EA029209B1 (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP11306321 2011-10-12
EP11306400.0A EP2586791A1 (en) 2011-10-28 2011-10-28 Gene cluster for biosynthesis of griselimycin and methylgriselimycin
PCT/EP2012/070213 WO2013053857A2 (en) 2011-10-12 2012-10-11 Gene cluster for biosynthesis of griselimycin and methylgriselimycin

Publications (2)

Publication Number Publication Date
EA201490767A1 EA201490767A1 (ru) 2014-09-30
EA029209B1 true EA029209B1 (ru) 2018-02-28

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
EA201490767A EA029209B1 (ru) 2011-10-12 2012-10-11 Кластер генов биосинтеза гризелимицина и метилгризелимицина

Country Status (7)

Country Link
US (1) US9422566B2 (enExample)
EP (1) EP2766389B1 (enExample)
JP (1) JP6430250B2 (enExample)
CN (1) CN104024272B (enExample)
EA (1) EA029209B1 (enExample)
IN (1) IN2014CN03414A (enExample)
WO (1) WO2013053857A2 (enExample)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EA029209B1 (ru) 2011-10-12 2018-02-28 Санофи Кластер генов биосинтеза гризелимицина и метилгризелимицина
WO2016196319A1 (en) * 2015-05-29 2016-12-08 Radiant Genomics, Inc. Compositions and methods for activating silent gene clusters
JP6821598B2 (ja) 2015-12-07 2021-01-27 ザイマージェン インコーポレイテッド Ｃｏｒｙｎｅｂａｃｔｅｒｉｕｍ ｇｌｕｔａｍｉｃｕｍ由来のプロモーター
US9988624B2 (en) 2015-12-07 2018-06-05 Zymergen Inc. Microbial strain improvement by a HTP genomic engineering platform
WO2017205387A1 (en) * 2016-05-23 2017-11-30 Northwestern University Systems and methods for untargeted metabolomic screening
US10544411B2 (en) 2016-06-30 2020-01-28 Zymergen Inc. Methods for generating a glucose permease library and uses thereof
EP3478833A4 (en) 2016-06-30 2019-10-02 Zymergen, Inc. METHOD FOR PRODUCING A BACTERIAL HEMOGLOBIN LIBRARY AND ITS USE

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3857937A (en) * 1971-02-25 1974-12-31 Rhone Poulenc Sa Antibiotics 23,671 rp and 23,672 rp
WO2000024775A1 (en) * 1998-10-23 2000-05-04 Galilaeus Oy Gene cluster involved in nogalamycin biosynthesis, and its use in production of hybrid antibiotics

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7078185B2 (en) * 2000-10-13 2006-07-18 Ecopia Biosciences Inc. Gene encoding a nonribosomal peptide synthetase for the production of ramoplanin
US20050027113A1 (en) * 2001-08-06 2005-02-03 Miao Vivian Pak Woon Compositions and methods relating to the daptomycin biosynthetic gene cluster
CN1388252A (zh) * 2002-08-06 2003-01-01 中国医学科学院医药生物技术研究所 吸水链霉菌17997生物合成格尔德霉素的基因簇
CN102021187A (zh) * 2003-10-23 2011-04-20 上海交通大学 Fr-008聚酮抗生素生物合成相关的基因簇
CN101016533B (zh) * 2005-11-09 2010-05-19 上海医药工业研究院 一种蒽环类抗生素产生工程菌及其应用
WO2009019524A1 (en) * 2007-08-03 2009-02-12 Naicons S.C.A.R.L. Genes and proteins for the biosynthesis of the lantibiotic 107891
CN101586112B (zh) * 2009-06-19 2011-06-22 中国科学院上海有机化学研究所 诺丝七肽的生物合成基因簇
EA029209B1 (ru) 2011-10-12 2018-02-28 Санофи Кластер генов биосинтеза гризелимицина и метилгризелимицина

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3857937A (en) * 1971-02-25 1974-12-31 Rhone Poulenc Sa Antibiotics 23,671 rp and 23,672 rp
WO2000024775A1 (en) * 1998-10-23 2000-05-04 Galilaeus Oy Gene cluster involved in nogalamycin biosynthesis, and its use in production of hybrid antibiotics

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BIGNELL DAWN R. D. ET AL.: "Streptomyces scabies 87-22 Contains a Coronafacic Acid-Like Biosynthetic Cluster That Contributes to Plant-Microbe Interactions", MOLECULAR PLANT-MICROBE INTERACTIONS, AMERICAN PHYTOPATHOLOGICAL SOCIETY, UNITED STATES, vol. 23, no. 2, 1 February 2010 (2010-02-01), pages 161-175, XP002674193, ISSN: 0894-0282, DOI: DOI:10.1094/MPMI-23-2-0161, page 163, right-hand column, paragraph 2 *
KATZ E. ET AL.: "Novel actinomycins formed by biosynthetic incorporation of cis- and trans-4-methylproline", ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, AMERICAN SOCIETY FOR MICROBIOLOGY, WASHINGTON, DC, US, vol. 11, no. 6, 1 June 1977 (1977-06-01), ages 1056-1063, XP002674194, ISSN: 0066-4804, page 1056, right-hand column, lines 1-4 *
MEIFENG TAO ET AL.: "The tallysomycin biosynthetic gene cluster from Streptoalloteichus hindustanus E465-94, ATCC 31158 unveiling new insights into the biosynthesis of the bleomycin family of antitumor antibiotics", MOLECULAR BIOSYSTEMS, ROYAL SOCIETY OF CHEMISTRY, GB, vol. 3, no. 1, 1 January 2007 (2007-01-01), pages 60-74, XP002674192, ISSN: 1742-206X, DOI: 10.1039/B615284H [retrieved on 2006-11-28], the whole document *
TERLAIN B. ET AL.: "[Structure of griselimycin, polypeptide antibiotic extracted from streptomyces cultures. 3. Products related to griselimycin]", BULLETIN DE LA SOCIETE CHIMIQUE DE FRANCE, SOCIETE FRANCAISE DE CHIMIE. PARIS, FRANCE, vol. 6, June 1971 (1971-06), pages 2363-2365, XP009158537, ISSN: 0037-8968, the whole document *
TOYOHARA ET AL.: "Aspects of the antituberculous activity of 27753-RP, a new semisynthetic derivative of griselimycine", ANNALES DE L'INSTITUT PASTEUR/MICROBIOLOGIE, PARIS, FR, vol. 138, no. 6, 1 November 1987 (1987-11-01), pages 737-744, XP023937378, ISSN: 0769-2609, DOI: 10.1016/0769-2609(87)90151-7 [retrieved on 1987-11-01], page 738, paragraph 2-3, page 743, paragraph 1-3 *

Also Published As

Publication number Publication date
IN2014CN03414A (enExample) 2015-10-09
EP2766389B1 (en) 2017-03-29
JP6430250B2 (ja) 2018-11-28
CN104024272B (zh) 2018-04-20
EA201490767A1 (ru) 2014-09-30
US9422566B2 (en) 2016-08-23
US20140295457A1 (en) 2014-10-02
WO2013053857A2 (en) 2013-04-18
EP2766389A2 (en) 2014-08-20
CN104024272A (zh) 2014-09-03
JP2014530609A (ja) 2014-11-20
WO2013053857A3 (en) 2013-08-01

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