EA013337B1 - Способ быстрой идентификации микотоксинов - Google Patents
Способ быстрой идентификации микотоксинов Download PDFInfo
- Publication number
- EA013337B1 EA013337B1 EA200801588A EA200801588A EA013337B1 EA 013337 B1 EA013337 B1 EA 013337B1 EA 200801588 A EA200801588 A EA 200801588A EA 200801588 A EA200801588 A EA 200801588A EA 013337 B1 EA013337 B1 EA 013337B1
- Authority
- EA
- Eurasian Patent Office
- Prior art keywords
- mycotoxins
- layer
- mycotoxin
- waveguide
- thin
- Prior art date
Links
- 239000002636 mycotoxin Substances 0.000 title claims abstract description 175
- 231100000678 Mycotoxin Toxicity 0.000 title claims abstract description 172
- 238000000034 method Methods 0.000 title claims abstract description 64
- 239000000126 substance Substances 0.000 claims abstract description 11
- 230000003993 interaction Effects 0.000 claims abstract description 6
- 235000013339 cereals Nutrition 0.000 claims description 30
- 239000002253 acid Substances 0.000 claims description 28
- 150000007513 acids Chemical class 0.000 claims description 26
- 239000000284 extract Substances 0.000 claims description 20
- MBMQEIFVQACCCH-UHFFFAOYSA-N trans-Zearalenon Natural products O=C1OC(C)CCCC(=O)CCCC=CC2=CC(O)=CC(O)=C21 MBMQEIFVQACCCH-UHFFFAOYSA-N 0.000 claims description 18
- MBMQEIFVQACCCH-QBODLPLBSA-N zearalenone Chemical compound O=C1O[C@@H](C)CCCC(=O)CCC\C=C\C2=CC(O)=CC(O)=C21 MBMQEIFVQACCCH-QBODLPLBSA-N 0.000 claims description 18
- LINOMUASTDIRTM-QGRHZQQGSA-N deoxynivalenol Chemical compound C([C@@]12[C@@]3(C[C@@H](O)[C@H]1O[C@@H]1C=C(C([C@@H](O)[C@@]13CO)=O)C)C)O2 LINOMUASTDIRTM-QGRHZQQGSA-N 0.000 claims description 15
- 229930002954 deoxynivalenol Natural products 0.000 claims description 15
- LINOMUASTDIRTM-UHFFFAOYSA-N vomitoxin hydrate Natural products OCC12C(O)C(=O)C(C)=CC1OC1C(O)CC2(C)C11CO1 LINOMUASTDIRTM-UHFFFAOYSA-N 0.000 claims description 15
- 235000013305 food Nutrition 0.000 claims description 14
- 239000000203 mixture Substances 0.000 claims description 14
- 238000002967 competitive immunoassay Methods 0.000 claims description 11
- ZRWPUFFVAOMMNM-UHFFFAOYSA-N Patulin Chemical compound OC1OCC=C2OC(=O)C=C12 ZRWPUFFVAOMMNM-UHFFFAOYSA-N 0.000 claims description 10
- -1 ergot alkaloids Natural products 0.000 claims description 10
- 239000003053 toxin Substances 0.000 claims description 10
- BXFOFFBJRFZBQZ-QYWOHJEZSA-N T-2 toxin Chemical compound C([C@@]12[C@]3(C)[C@H](OC(C)=O)[C@@H](O)[C@H]1O[C@H]1[C@]3(COC(C)=O)C[C@@H](C(=C1)C)OC(=O)CC(C)C)O2 BXFOFFBJRFZBQZ-QYWOHJEZSA-N 0.000 claims description 9
- 102000004169 proteins and genes Human genes 0.000 claims description 9
- 108090000623 proteins and genes Proteins 0.000 claims description 9
- 231100000765 toxin Toxicity 0.000 claims description 9
- 108700012359 toxins Proteins 0.000 claims description 9
- ITCSWEBPTQLQKN-UHFFFAOYSA-N Nivalenol Natural products CC1=CC2OC3C(O)C(O)C(C2(CO)CC1=O)C34CO4 ITCSWEBPTQLQKN-UHFFFAOYSA-N 0.000 claims description 8
- UKOTXHQERFPCBU-YQPARWETSA-N Nivalenol Chemical compound C([C@]12[C@@]3([C@H](O)[C@@H](O)[C@H]1O[C@@H]1C=C(C([C@@H](O)[C@@]13CO)=O)C)C)O2 UKOTXHQERFPCBU-YQPARWETSA-N 0.000 claims description 8
- VYLQGYLYRQKMFU-UHFFFAOYSA-N Ochratoxin A Natural products CC1Cc2c(Cl)cc(CNC(Cc3ccccc3)C(=O)O)cc2C(=O)O1 VYLQGYLYRQKMFU-UHFFFAOYSA-N 0.000 claims description 8
- RWQKHEORZBHNRI-BMIGLBTASA-N ochratoxin A Chemical compound C([C@H](NC(=O)C1=CC(Cl)=C2C[C@H](OC(=O)C2=C1O)C)C(O)=O)C1=CC=CC=C1 RWQKHEORZBHNRI-BMIGLBTASA-N 0.000 claims description 8
- DAEYIVCTQUFNTM-UHFFFAOYSA-N ochratoxin B Natural products OC1=C2C(=O)OC(C)CC2=CC=C1C(=O)NC(C(O)=O)CC1=CC=CC=C1 DAEYIVCTQUFNTM-UHFFFAOYSA-N 0.000 claims description 8
- 239000002904 solvent Substances 0.000 claims description 8
- FTMKAMVLFVRZQX-UHFFFAOYSA-N octadecylphosphonic acid Chemical compound CCCCCCCCCCCCCCCCCCP(O)(O)=O FTMKAMVLFVRZQX-UHFFFAOYSA-N 0.000 claims description 7
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 claims description 6
- 241000223218 Fusarium Species 0.000 claims description 6
- TVACALAUIQMRDF-UHFFFAOYSA-N dodecyl dihydrogen phosphate Chemical compound CCCCCCCCCCCCOP(O)(O)=O TVACALAUIQMRDF-UHFFFAOYSA-N 0.000 claims description 6
- 235000013399 edible fruits Nutrition 0.000 claims description 6
- 150000003839 salts Chemical class 0.000 claims description 6
- 235000014101 wine Nutrition 0.000 claims description 6
- 229930195730 Aflatoxin Natural products 0.000 claims description 5
- 239000005409 aflatoxin Substances 0.000 claims description 5
- 229960003133 ergot alkaloid Drugs 0.000 claims description 5
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 5
- 239000003008 fumonisin Substances 0.000 claims description 5
- 238000003018 immunoassay Methods 0.000 claims description 5
- PNKLMTPXERFKEN-ZIOSACBISA-N mycotoxin ht 2 Chemical compound C([C@]12[C@]3(C)[C@H](O)[C@@H](O)[C@H]1O[C@H]1[C@]3(COC(C)=O)C[C@@H](C(=C1)C)OC(=O)CC(C)C)O2 PNKLMTPXERFKEN-ZIOSACBISA-N 0.000 claims description 5
- 229930183344 ochratoxin Natural products 0.000 claims description 5
- 238000002360 preparation method Methods 0.000 claims description 5
- PXFBZOLANLWPMH-UHFFFAOYSA-N 16-Epiaffinine Natural products C1C(C2=CC=CC=C2N2)=C2C(=O)CC2C(=CC)CN(C)C1C2CO PXFBZOLANLWPMH-UHFFFAOYSA-N 0.000 claims description 4
- UXDPXZQHTDAXOZ-UHFFFAOYSA-N Fumonisin B2 Natural products OC(=O)CC(C(O)=O)CC(=O)OC(C(C)CCCC)C(OC(=O)CC(CC(O)=O)C(O)=O)CC(C)CCCCCCC(O)CC(O)C(C)N UXDPXZQHTDAXOZ-UHFFFAOYSA-N 0.000 claims description 4
- CPCRJSQNWHCGOP-KUHXKYQKSA-N Fumonisin B3 Chemical compound OC(=O)CC(C(O)=O)CC(=O)O[C@H]([C@H](C)CCCC)[C@@H](OC(=O)CC(CC(O)=O)C(O)=O)C[C@@H](C)C[C@H](O)CCCCCC[C@@H](O)[C@H](C)N CPCRJSQNWHCGOP-KUHXKYQKSA-N 0.000 claims description 4
- 241001465754 Metazoa Species 0.000 claims description 4
- 125000000217 alkyl group Chemical group 0.000 claims description 4
- UVBUBMSSQKOIBE-DSLOAKGESA-N fumonisin B1 Chemical compound OC(=O)C[C@@H](C(O)=O)CC(=O)O[C@H]([C@H](C)CCCC)[C@@H](OC(=O)C[C@@H](CC(O)=O)C(O)=O)C[C@@H](C)C[C@H](O)CCCC[C@@H](O)C[C@H](O)[C@H](C)N UVBUBMSSQKOIBE-DSLOAKGESA-N 0.000 claims description 4
- QZIADBYRQILELJ-UHFFFAOYSA-N fumonisin B1 Natural products CCCCC(C)C(OC(=O)CC(CC(=O)O)C(=O)O)C(C)(CC(C)CC(O)CCCCC(O)CC(O)C(C)N)OC(=O)CC(CC(=O)O)C(=O)O QZIADBYRQILELJ-UHFFFAOYSA-N 0.000 claims description 4
- UXDPXZQHTDAXOZ-STOIETHLSA-N fumonisin B2 Chemical compound OC(=O)C[C@@H](C(O)=O)CC(=O)O[C@H]([C@H](C)CCCC)[C@@H](OC(=O)C[C@@H](CC(O)=O)C(O)=O)C[C@@H](C)CCCCCC[C@@H](O)C[C@H](O)[C@H](C)N UXDPXZQHTDAXOZ-STOIETHLSA-N 0.000 claims description 4
- CPCRJSQNWHCGOP-UHFFFAOYSA-N fumonisin B3 Natural products OC(=O)CC(C(O)=O)CC(=O)OC(C(C)CCCC)C(OC(=O)CC(CC(O)=O)C(O)=O)CC(C)CC(O)CCCCCCC(O)C(C)N CPCRJSQNWHCGOP-UHFFFAOYSA-N 0.000 claims description 4
- GBHRVZIGDIUCJB-UHFFFAOYSA-N hydrogenphosphite Chemical class OP([O-])[O-] GBHRVZIGDIUCJB-UHFFFAOYSA-N 0.000 claims description 4
- CSYVMZMEBKUDRQ-BNWDXEGFSA-N nt-2 toxin Chemical compound C([C@@]12[C@@]3(C)[C@@]4(CO)C[C@H](O)C(C)=C[C@H]4O[C@@H]1[C@H](O)[C@H]3OC(=O)C)O2 CSYVMZMEBKUDRQ-BNWDXEGFSA-N 0.000 claims description 4
- 108091003079 Bovine Serum Albumin Proteins 0.000 claims description 3
- 229940098773 bovine serum albumin Drugs 0.000 claims description 3
- 108091023037 Aptamer Proteins 0.000 claims description 2
- 239000012634 fragment Substances 0.000 claims description 2
- 229920000578 graft copolymer Polymers 0.000 claims description 2
- 239000003550 marker Substances 0.000 claims description 2
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 2
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 2
- 230000005855 radiation Effects 0.000 claims 1
- 238000001514 detection method Methods 0.000 abstract description 8
- 239000010409 thin film Substances 0.000 abstract 3
- 239000010410 layer Substances 0.000 description 104
- 238000000018 DNA microarray Methods 0.000 description 23
- 239000000243 solution Substances 0.000 description 22
- 230000003287 optical effect Effects 0.000 description 17
- 230000008901 benefit Effects 0.000 description 13
- 238000004458 analytical method Methods 0.000 description 12
- 238000005259 measurement Methods 0.000 description 12
- 238000011534 incubation Methods 0.000 description 9
- 238000005406 washing Methods 0.000 description 9
- 235000018102 proteins Nutrition 0.000 description 8
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- IMNFDUFMRHMDMM-UHFFFAOYSA-N N-Heptane Chemical compound CCCCCCC IMNFDUFMRHMDMM-UHFFFAOYSA-N 0.000 description 6
- 238000012545 processing Methods 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 230000008859 change Effects 0.000 description 5
- 238000000605 extraction Methods 0.000 description 5
- 239000011521 glass Substances 0.000 description 5
- 238000004128 high performance liquid chromatography Methods 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 4
- 241000209140 Triticum Species 0.000 description 4
- 235000021307 Triticum Nutrition 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 125000004122 cyclic group Chemical group 0.000 description 4
- 230000005284 excitation Effects 0.000 description 4
- 229940127121 immunoconjugate Drugs 0.000 description 4
- 238000002372 labelling Methods 0.000 description 4
- BPUBBGLMJRNUCC-UHFFFAOYSA-N oxygen(2-);tantalum(5+) Chemical compound [O-2].[O-2].[O-2].[O-2].[O-2].[Ta+5].[Ta+5] BPUBBGLMJRNUCC-UHFFFAOYSA-N 0.000 description 4
- 229920000098 polyolefin Polymers 0.000 description 4
- 239000002356 single layer Substances 0.000 description 4
- PBCFLUZVCVVTBY-UHFFFAOYSA-N tantalum pentoxide Inorganic materials O=[Ta](=O)O[Ta](=O)=O PBCFLUZVCVVTBY-UHFFFAOYSA-N 0.000 description 4
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
- 239000007864 aqueous solution Substances 0.000 description 3
- 238000003556 assay Methods 0.000 description 3
- 239000007853 buffer solution Substances 0.000 description 3
- 230000002860 competitive effect Effects 0.000 description 3
- 238000004590 computer program Methods 0.000 description 3
- 238000007598 dipping method Methods 0.000 description 3
- 235000013312 flour Nutrition 0.000 description 3
- 238000004020 luminiscence type Methods 0.000 description 3
- 229920003023 plastic Polymers 0.000 description 3
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 2
- GDOHDNVLNKFRDG-UHFFFAOYSA-N CCCCCCCCCCCCCCCCCCOP(O)=O Chemical compound CCCCCCCCCCCCCCCCCCOP(O)=O GDOHDNVLNKFRDG-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 101100063504 Mus musculus Dlx2 gene Proteins 0.000 description 2
- 239000000020 Nitrocellulose Substances 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- 239000002202 Polyethylene glycol Substances 0.000 description 2
- 239000004642 Polyimide Substances 0.000 description 2
- 239000004793 Polystyrene Substances 0.000 description 2
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 2
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 2
- 239000011324 bead Substances 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000012875 competitive assay Methods 0.000 description 2
- 229920001577 copolymer Polymers 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 239000007850 fluorescent dye Substances 0.000 description 2
- 102000034287 fluorescent proteins Human genes 0.000 description 2
- 108091006047 fluorescent proteins Proteins 0.000 description 2
- 239000002105 nanoparticle Substances 0.000 description 2
- 229920001220 nitrocellulos Polymers 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 239000004417 polycarbonate Substances 0.000 description 2
- 229920000515 polycarbonate Polymers 0.000 description 2
- 229920001223 polyethylene glycol Polymers 0.000 description 2
- 229920001721 polyimide Polymers 0.000 description 2
- 229920002223 polystyrene Polymers 0.000 description 2
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 1
- HBAQYPYDRFILMT-UHFFFAOYSA-N 8-[3-(1-cyclopropylpyrazol-4-yl)-1H-pyrazolo[4,3-d]pyrimidin-5-yl]-3-methyl-3,8-diazabicyclo[3.2.1]octan-2-one Chemical class C1(CC1)N1N=CC(=C1)C1=NNC2=C1N=C(N=C2)N1C2C(N(CC1CC2)C)=O HBAQYPYDRFILMT-UHFFFAOYSA-N 0.000 description 1
- NIXOWILDQLNWCW-UHFFFAOYSA-M Acrylate Chemical compound [O-]C(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-M 0.000 description 1
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- 235000004570 Agaricus campestris Nutrition 0.000 description 1
- 240000007440 Agaricus campestris Species 0.000 description 1
- 108010032595 Antibody Binding Sites Proteins 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 102000014914 Carrier Proteins Human genes 0.000 description 1
- 108010078791 Carrier Proteins Proteins 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 229910018828 PO3H2 Inorganic materials 0.000 description 1
- 229920002319 Poly(methyl acrylate) Polymers 0.000 description 1
- 108010071390 Serum Albumin Proteins 0.000 description 1
- 102000007562 Serum Albumin Human genes 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 231100000570 acute poisoning Toxicity 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 239000006172 buffering agent Substances 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 231100000739 chronic poisoning Toxicity 0.000 description 1
- 239000007979 citrate buffer Substances 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000005206 flow analysis Methods 0.000 description 1
- 238000001917 fluorescence detection Methods 0.000 description 1
- 125000003827 glycol group Chemical group 0.000 description 1
- 235000011868 grain product Nutrition 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 229910052740 iodine Inorganic materials 0.000 description 1
- 239000011630 iodine Substances 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 238000004890 malting Methods 0.000 description 1
- DBOAVDSSZWDGTH-UHFFFAOYSA-N n-(4-butylphenyl)-1-(4-ethoxyphenyl)methanimine Chemical compound C1=CC(CCCC)=CC=C1N=CC1=CC=C(OCC)C=C1 DBOAVDSSZWDGTH-UHFFFAOYSA-N 0.000 description 1
- 231100000763 ototoxin Toxicity 0.000 description 1
- 230000000737 periodic effect Effects 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 229920000193 polymethacrylate Polymers 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000011533 pre-incubation Methods 0.000 description 1
- 230000006916 protein interaction Effects 0.000 description 1
- 239000012460 protein solution Substances 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000004451 qualitative analysis Methods 0.000 description 1
- 239000010453 quartz Substances 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 150000004760 silicates Chemical class 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 235000020183 skimmed milk Nutrition 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 229920006352 transparent thermoplastic Polymers 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/7703—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator using reagent-clad optical fibres or optical waveguides
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/648—Specially adapted constructive features of fluorimeters using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N2021/7769—Measurement method of reaction-produced change in sensor
- G01N2021/7786—Fluorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/37—Assays involving biological materials from specific organisms or of a specific nature from fungi
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Engineering & Computer Science (AREA)
- General Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Pathology (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Plasma & Fusion (AREA)
- Optics & Photonics (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Cephalosporin Compounds (AREA)
- Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE102005062377A DE102005062377A1 (de) | 2005-12-23 | 2005-12-23 | Vorrichtung und Verfahren zum Nachweis von Mykotoxinen |
| PCT/EP2006/012000 WO2007079893A1 (de) | 2005-12-23 | 2006-12-13 | Vorrichtung und verfahren zum nachweis von mykotoxinen |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| EA200801588A1 EA200801588A1 (ru) | 2008-12-30 |
| EA013337B1 true EA013337B1 (ru) | 2010-04-30 |
Family
ID=37909504
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EA200801588A EA013337B1 (ru) | 2005-12-23 | 2006-12-13 | Способ быстрой идентификации микотоксинов |
Country Status (19)
| Country | Link |
|---|---|
| US (1) | US20090081808A1 (enExample) |
| EP (1) | EP1966594B1 (enExample) |
| JP (1) | JP2009520956A (enExample) |
| KR (1) | KR101232499B1 (enExample) |
| CN (1) | CN101384896A (enExample) |
| AR (1) | AR058616A1 (enExample) |
| AU (1) | AU2006334802B2 (enExample) |
| BR (1) | BRPI0620376A2 (enExample) |
| CA (1) | CA2634559A1 (enExample) |
| CR (1) | CR10087A (enExample) |
| DE (1) | DE102005062377A1 (enExample) |
| DK (1) | DK1966594T3 (enExample) |
| EA (1) | EA013337B1 (enExample) |
| ES (1) | ES2400919T3 (enExample) |
| PL (1) | PL1966594T3 (enExample) |
| PT (1) | PT1966594E (enExample) |
| UA (1) | UA97792C2 (enExample) |
| WO (1) | WO2007079893A1 (enExample) |
| ZA (1) | ZA200805457B (enExample) |
Families Citing this family (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102009016712A1 (de) | 2009-04-09 | 2010-10-14 | Bayer Technology Services Gmbh | Einweg-Mikrofluidik-Testkassette zur Bioassay von Analyten |
| AP2011005905A0 (en) | 2009-04-09 | 2011-10-31 | Bayer Cropscience Ag | Device and method for the verification and quantitative analysis of analytes, particularly mycotoxins. |
| WO2011023230A1 (en) * | 2009-08-27 | 2011-03-03 | Foss Analytical Ab | Method of extracting mycotoxins from cereal grains |
| CN101666752B (zh) * | 2009-09-29 | 2011-07-20 | 济南大学 | 一种糖基功能化细菌毒素分子印迹柱的制备方法及应用 |
| JP5840845B2 (ja) * | 2011-02-25 | 2016-01-06 | 国立研究開発法人農業・食品産業技術総合研究機構 | 危害要因定量方法、危害要因定量装置、および、プログラム |
| ITMO20110109A1 (it) * | 2011-05-11 | 2012-11-12 | Generon S R L | Metodo per l'analisi di aflatossine nel latte e nei derivati del latte |
| CN102279268A (zh) * | 2011-07-29 | 2011-12-14 | 上海交通大学 | 同时检测玉米赤霉烯酮与伏马毒素的方法 |
| CN102443585A (zh) * | 2011-11-25 | 2012-05-09 | 国家纳米技术与工程研究院 | 玉米赤霉烯酮核酸适体及其应用 |
| CN102517290A (zh) * | 2011-11-25 | 2012-06-27 | 国家纳米技术与工程研究院 | 伏马毒素b2核酸适体及其应用 |
| EP2810070B1 (en) * | 2012-02-03 | 2020-04-01 | Charm Sciences Inc. | Extraction of mycotoxins |
| JP5731016B2 (ja) * | 2012-04-27 | 2015-06-10 | キリン株式会社 | リガンドを高感度に検出する核酸分子並びに該核酸分子のスクリーニング方法および該核酸分子の感度の最適化方法 |
| CN103743913B (zh) * | 2014-01-23 | 2015-07-08 | 福建农林大学 | 快速鉴定与黄曲霉毒素b1互作的宿主蛋白的方法 |
| CN104293793B (zh) * | 2014-07-24 | 2018-05-18 | 江南大学 | 一种特异识别t-2毒素的寡核苷酸适配体 |
| FR3028318B1 (fr) * | 2014-11-12 | 2025-10-03 | Phuong Lan Tran | Procede et dispositif de tri selectif, specifique et simultane de cellules rares cibles dans un echantillon biologique |
| CN104593374B (zh) * | 2015-03-02 | 2017-05-17 | 江南大学 | 一种特异识别棒曲霉素的寡核苷酸适配体 |
| EP3294750B1 (en) | 2015-05-08 | 2023-10-25 | Waters Technologies Corporation | Composition and methods for extracting mycotoxins |
| KR102042661B1 (ko) * | 2015-08-06 | 2019-11-08 | 광주과학기술원 | 타겟 물질 검출용 복합체 및 이를 이용한 타겟 물질 검출방법 |
| DE202016106024U1 (de) | 2016-10-26 | 2016-11-08 | Matthias Godejohann | Vorrichtung zur Halterung zu einer Halbleiterplatte |
| CN108614054A (zh) * | 2016-12-12 | 2018-10-02 | 北京农学院 | 基于聚苯乙烯-二乙烯苯树脂为载体的真菌毒素免疫亲和柱的制备方法 |
| CN108490169B (zh) * | 2018-03-27 | 2021-09-03 | 广东一家人食品有限公司 | 一种米粉中真菌毒素的双水相萃取联合酶联免疫检测的方法 |
| BR112021025933A2 (pt) | 2019-07-03 | 2022-02-08 | Intervet Int Bv | Desoxinivalenol conjugado para proteger contra micotoxicose |
| JP7387524B2 (ja) * | 2020-04-06 | 2023-11-28 | Ｔｉａｎｍａ Ｊａｐａｎ株式会社 | 蛍光を用いた免疫分析法で測定するための試料溶液の調製方法、測定用セル、測定キットおよび試料溶液の調製装置 |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000043552A2 (en) * | 1999-01-25 | 2000-07-27 | Ut-Battelle, Llc | Multifunctional and multispectral biosensor devices and methods of use |
| WO2002020873A2 (de) * | 2000-09-05 | 2002-03-14 | Zeptosens Ag | Verfahren zur abscheidung von mono- und mehrfachschichten von organophosphor- und -phosphonsäuren und deren salzen sowie deren verwendung |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4208479A (en) * | 1977-07-14 | 1980-06-17 | Syva Company | Label modified immunoassays |
| US5178832A (en) * | 1987-09-28 | 1993-01-12 | The Texas A&M University System | Selective immobilization and detection of mycotoxins in solution |
| US5429952A (en) * | 1988-02-02 | 1995-07-04 | Biocode, Inc. | Marking of products to establish identity and source |
| US5814565A (en) * | 1995-02-23 | 1998-09-29 | University Of Utah Research Foundation | Integrated optic waveguide immunosensor |
| EP1057008B1 (de) * | 1998-02-05 | 2009-09-09 | Novartis AG | Verfahren und vorrichtung zur lumineszenzmessung |
| US7396675B2 (en) * | 2000-06-02 | 2008-07-08 | Bayer Technology Services Gmbh | Kit and method for determining a plurality of analytes |
| US6812380B2 (en) * | 2001-03-27 | 2004-11-02 | Pioneer Hi-Bred International, Inc. | Compositions and methods of zearalenone detoxification |
| JP4203826B2 (ja) * | 2003-09-29 | 2009-01-07 | 独立行政法人産業技術総合研究所 | 自動分析方法及び装置 |
| US20060210425A1 (en) * | 2005-03-21 | 2006-09-21 | Laura Mirkarimi | Inorganic coatings for optical and other applications |
-
2005
- 2005-12-23 DE DE102005062377A patent/DE102005062377A1/de not_active Withdrawn
-
2006
- 2006-12-13 CN CNA2006800532011A patent/CN101384896A/zh active Pending
- 2006-12-13 PT PT68295740T patent/PT1966594E/pt unknown
- 2006-12-13 WO PCT/EP2006/012000 patent/WO2007079893A1/de not_active Ceased
- 2006-12-13 AU AU2006334802A patent/AU2006334802B2/en not_active Ceased
- 2006-12-13 BR BRPI0620376-0A patent/BRPI0620376A2/pt not_active IP Right Cessation
- 2006-12-13 DK DK06829574.0T patent/DK1966594T3/da active
- 2006-12-13 KR KR1020087018026A patent/KR101232499B1/ko not_active Expired - Fee Related
- 2006-12-13 UA UAA200809665A patent/UA97792C2/ru unknown
- 2006-12-13 US US12/158,788 patent/US20090081808A1/en not_active Abandoned
- 2006-12-13 ES ES06829574T patent/ES2400919T3/es active Active
- 2006-12-13 CA CA002634559A patent/CA2634559A1/en not_active Abandoned
- 2006-12-13 PL PL06829574T patent/PL1966594T3/pl unknown
- 2006-12-13 EA EA200801588A patent/EA013337B1/ru not_active IP Right Cessation
- 2006-12-13 JP JP2008546202A patent/JP2009520956A/ja active Pending
- 2006-12-13 EP EP06829574A patent/EP1966594B1/de not_active Not-in-force
- 2006-12-21 AR ARP060105723A patent/AR058616A1/es not_active Application Discontinuation
-
2008
- 2008-06-19 CR CR10087A patent/CR10087A/es unknown
- 2008-06-23 ZA ZA200805457A patent/ZA200805457B/xx unknown
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000043552A2 (en) * | 1999-01-25 | 2000-07-27 | Ut-Battelle, Llc | Multifunctional and multispectral biosensor devices and methods of use |
| WO2002020873A2 (de) * | 2000-09-05 | 2002-03-14 | Zeptosens Ag | Verfahren zur abscheidung von mono- und mehrfachschichten von organophosphor- und -phosphonsäuren und deren salzen sowie deren verwendung |
Non-Patent Citations (3)
| Title |
|---|
| LIGLER FRANCES S. ET AL.: "Array biosensor for detection of toxins." ANALYTICAL AND BIOANALYTICAL CHEMISTRY OCT 2003, vol. 377, no. 3, October 2003 (2003-10), pages 469-477, XP002430514 ISSN: 1618-2642 the whole document * |
| MARK J FELDSTEIN ET AL.: "Array Biosensor: Optical and Fluidics Systems" BIOMEDICAL MICRODEVICES, KLUWER ACADEMIC PUBLISHERS, BO, vol. 1, no. 2, 1 December 1998 (1998-12-01), pages 139-153, XP019205048 ISSN: 1572-8781 page 139, column 2, paragraph 3 - page 141, column 2, paragraph 1 * |
| NGUNDI MIRIAM M. ET AL.: "Array biosensor for detection of ochratoxin A in cereals and beverages." ANALYTICAL CHEMISTRY 1 JAN 2005, vol. 77, no. 1, 1 January 2005 (2005-01-01), pages 148-154, XP002430515 ISSN: 0003-2700 cited in the application the whole document * |
Also Published As
| Publication number | Publication date |
|---|---|
| PT1966594E (pt) | 2013-03-13 |
| AR058616A1 (es) | 2008-02-13 |
| EP1966594B1 (de) | 2013-01-23 |
| UA97792C2 (ru) | 2012-03-26 |
| AU2006334802A1 (en) | 2007-07-19 |
| KR20080083682A (ko) | 2008-09-18 |
| ZA200805457B (en) | 2010-02-24 |
| AU2006334802B2 (en) | 2013-03-14 |
| DE102005062377A1 (de) | 2007-06-28 |
| CR10087A (es) | 2009-01-27 |
| KR101232499B1 (ko) | 2013-02-12 |
| JP2009520956A (ja) | 2009-05-28 |
| EA200801588A1 (ru) | 2008-12-30 |
| BRPI0620376A2 (pt) | 2011-11-08 |
| EP1966594A1 (de) | 2008-09-10 |
| DK1966594T3 (da) | 2013-04-15 |
| CN101384896A (zh) | 2009-03-11 |
| CA2634559A1 (en) | 2007-07-19 |
| ES2400919T3 (es) | 2013-04-15 |
| PL1966594T3 (pl) | 2013-06-28 |
| WO2007079893A1 (de) | 2007-07-19 |
| US20090081808A1 (en) | 2009-03-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EA013337B1 (ru) | Способ быстрой идентификации микотоксинов | |
| US5219763A (en) | Agglutination method for the determination of multiple ligands | |
| Zherdev et al. | Ways to reach lower detection limits of lateral flow immunoassays | |
| US20210072237A1 (en) | Method and device for determining biological analytes | |
| US20120316077A1 (en) | System And Method For Detection And Analysis Of A Molecule In A Sample | |
| JP7017310B2 (ja) | スポットに分析方法を実行するための対照マーカー | |
| KR101451733B1 (ko) | 아플라톡신 ｂ1 검출용 표지물질 및 이를 포함한 아플라톡신 ｂ1 검출용 키트 | |
| RU2379691C1 (ru) | Способ многоаналитного иммуноанализа с использованием микрочастиц | |
| WO2011032278A1 (en) | A method and apparatus for lateral flow determination of analyte concentration | |
| CN106198966A (zh) | 一种检测黄曲霉毒素的近红外荧光免疫层析试剂盒及其应用 | |
| US9146233B2 (en) | Detecting molecular interactions by fluorescence resonance energy transfer on a solid-phase support | |
| KR100908641B1 (ko) | 칩 기술을 기반으로 하는 경쟁적 상호작용을 이용한 비표지혈액 단백질의 분석방법 | |
| WO2009040364A1 (en) | Multiparameter assay | |
| Çimen et al. | Proteomic applications of plasmonic sensors | |
| WO2024137691A2 (en) | Fluorescent immunoassays enhanced using plasmonic nanostructures | |
| WO2006062427A1 (en) | Method for quantitatively detecting biological toxins | |
| RU2320994C1 (ru) | Способ количественного обнаружения биологических токсинов | |
| MX2008008004A (en) | Device and method for identifying mycotoxins | |
| RU2789545C1 (ru) | Способ высокочувствительного конкурентного иммунохроматографического анализа | |
| CN117881963A (zh) | 用于同时检测多种分析物的系统 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PC4A | Registration of transfer of a eurasian patent by assignment | ||
| MM4A | Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s) |
Designated state(s): AM AZ BY KG MD TJ TM |
|
| PC4A | Registration of transfer of a eurasian patent by assignment | ||
| MM4A | Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s) |
Designated state(s): KZ RU |